Haploid technology is a valuable plant breeding tool for obtaining homozygosity particularly in w... more Haploid technology is a valuable plant breeding tool for obtaining homozygosity particularly in woody plants. Hazelnut, the world's sixth ranking nut tree crop is a monoecious, anemophilous species. It is characterizes by a sporophytic incompatibility system that prevents production of plants with conventional methods, involving several self-pollination cycles. In this study, gametic embryogenesis, in particular isolated microspore culture of Olea europea was done with five genotypes. Two culture media were tested and four temperature stress were applied to the isolated microspore that were cultivated at vacuolated developmental stage. To our knowledge, this is the first report of early embryo being recovered from the five hazelnut genotypes via isolated microspore culture. Analysis of the embryo genotypes using SSR markers demonstrated the embryo had a single per each locus whereas the parent was heterozygous, indicating they developed from haploid microspores. the response to the culture treatments, was, however, genotype-dependent, as previously reported for male gamete embryogenesis for other fruit crops.
In order to investigate the seasonal variations of antimicrobial properties and chemical composit... more In order to investigate the seasonal variations of antimicrobial properties and chemical composition of essential oils (EOs), three different cultivars of Citrus limon L. Burm. spp. (Femminello Santa Teresa, Monachello and Femminello Continella) were collected at 6-week intervals, from December 2012 to April 2013, for a total of four harvests. The EOs were extracted from lemon peel by hydro-distillation. The antimicrobial activity, tested by paper disc diffusion method, was evaluated against common food-related pathogenic bacteria (Listeria monocytogenes, Staphylococcus aureus, Salmonella enterica and Enterobacter spp.). EOs were more effective against Gram-positive than Gram-negative bacteria at each collection time, but a strong strain dependence was evidenced. Monachello EOs showed the highest inhibition power. The chemical characterisation of the EOs performed by gas chromatography/mass spectrometry identified from 36 to 42 molecules. The chemical difference registered among samples and seasons may explain the different antimicrobial efficacies recorded.
Haploid technology is a valuable plant breeding tool for obtaining homozygosity particularly in w... more Haploid technology is a valuable plant breeding tool for obtaining homozygosity particularly in woody plants. Hazelnut, the world's sixth ranking nut tree crop is a monoecious, anemophilous species. It is characterizes by a sporophytic incompatibility system that prevents production of plants with conventional methods, involving several self-pollination cycles. In this study, gametic embryogenesis, in particular isolated microspore culture of Olea europea was done with five genotypes. Two culture media were tested and four temperature stress were applied to the isolated microspore that were cultivated at vacuolated developmental stage. To our knowledge, this is the first report of early embryo being recovered from the five hazelnut genotypes via isolated microspore culture. Analysis of the embryo genotypes using SSR markers demonstrated the embryo had a single per each locus whereas the parent was heterozygous, indicating they developed from haploid microspores. the response to the culture treatments, was, however, genotype-dependent, as previously reported for male gamete embryogenesis for other fruit crops.
In order to investigate the seasonal variations of antimicrobial properties and chemical composit... more In order to investigate the seasonal variations of antimicrobial properties and chemical composition of essential oils (EOs), three different cultivars of Citrus limon L. Burm. spp. (Femminello Santa Teresa, Monachello and Femminello Continella) were collected at 6-week intervals, from December 2012 to April 2013, for a total of four harvests. The EOs were extracted from lemon peel by hydro-distillation. The antimicrobial activity, tested by paper disc diffusion method, was evaluated against common food-related pathogenic bacteria (Listeria monocytogenes, Staphylococcus aureus, Salmonella enterica and Enterobacter spp.). EOs were more effective against Gram-positive than Gram-negative bacteria at each collection time, but a strong strain dependence was evidenced. Monachello EOs showed the highest inhibition power. The chemical characterisation of the EOs performed by gas chromatography/mass spectrometry identified from 36 to 42 molecules. The chemical difference registered among samples and seasons may explain the different antimicrobial efficacies recorded.
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The full paper is available at:
http://link.springer.com/article/10.1007%2Fs11240-015-0921-1
The full paper is available at:
http://link.springer.com/article/10.1007%2Fs11240-015-0921-1