Papers by Pedro D'Azevedo
SUMMARY - Traditionally the Laboratory of Clinical Microbiology uses the susceptibility test to t... more SUMMARY - Traditionally the Laboratory of Clinical Microbiology uses the susceptibility test to the novobiocin to distinguish the cli- nically significant species from SCoN, between them the Staphylococcus saprophyticus. Due to the increase of these microorganisms in the infections related to the health assistance, this study had as objective to relate two cases of bacteremia for SCoN novobiocin re- sistant occurred in May and September of 2006, in a general hospital, in the city of São Paulo. First the fenotipic test pointed resistan- ce to the novobiocin but with differents patterns of identification the S. saprophyticus. In the conventional identification, the isolates had leavend trealose, manitol and manose, being positive in the tests of urease and fosfatase alkaline. In the half-automatized system, the confirmation of the species pointed the Staphylococcus cohnii subsp urealyticus with 99.99% of probability. In the disk-diffusion test, the isolates had showed resistance to th...
Bookmarks Related papers MentionsView impact
The urinary tract infections (UTIs) represent the most common form of bacterial infections in pre... more The urinary tract infections (UTIs) represent the most common form of bacterial infections in pregnant women. Pregnancy is a situation that predisposes to the development of UTIs due to physiological changes (mechanical and hormonal) that occur in this period of women’s life, facilitating the transformation of asymptomatic bacteriuria women (AB) in symptomatic ones. AB affects 2-10% of pregnant women, and if not properly treated, it can develop pyelonephritis in 40% of cases. UTIs are clinically manifested by dysuria, urinary frequency, urinary urgency and lower abdominal pain in cystitis, chills and low back pain in pyelonephritis, or complete absence of symptoms in asymptomatic bacteriuria. The diagnosis in most cases is clinical, except for asymptomatic bacteriuria. One should take into account factors such as the patient’s condition, tolerability, and maternal and fetal toxicity when choosing the best therapeutic approach. The aim of this study was to review the work that addres...
Bookmarks Related papers MentionsView impact
The Brazilian Journal of Infectious Diseases, 2015
Bookmarks Related papers MentionsView impact
The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases, 2004
Rubella serum assays performed in the laboratory of the Materno-Infantil Presidente Vargas Hospit... more Rubella serum assays performed in the laboratory of the Materno-Infantil Presidente Vargas Hospital (HMIPV) from 1998 to 2002 were reviewed to determine if IgG avidity assays should be implemented. IgG was determined using the Enzyme Linked Fluorescent Assay, ELFA, VIDAS system, bioMerieux or the Microparticle Enzyme Immunoassay, MEIA, Axsym system, Abbott, and IgM was determined using the ELFA, VIDAS system, bioMerieux, a capture format assay. Specific IgG was assayed in 2,863 samples, with positive results for 84% of the patients, for the most part with high levels of antibodies. IgM was assayed in 2,851 samples, being positive in 14 (0.49%) and inconclusive in 25 (0.88%). Serology for toxoplasmosis was also positive or inconclusive in 5 patients. After a cost-effectiveness analysis, it was decided not to implement avidity assays, considering that the HMIPV is a public institution, with limited funding. Difficulties concerning the integration of the Clinical Pathology Service with...
Bookmarks Related papers MentionsView impact
Infection control and hospital epidemiology, 2004
Detection of colonized patients is important for implementing control measures for methicillin-re... more Detection of colonized patients is important for implementing control measures for methicillin-resistant Staphylococcus aureus (MRSA). Laboratory detection of MRSA carriers is increased by the use of selective screening media, helping control dissemination of such organisms. To evaluate three different media, including selective and nonselective media, in the detection of MRSA from clinical specimens of patients of an intensive care unit (ICU). Adult patients in the ICU of the Hospital Mãe de Deus, Porto Alegre, Brazil. A total of 224 specimens were obtained from the nares of patients and plated on blood agar, mannitol salt agar containing 2 microg/mL of oxacillin (MSAO), and oxacillin resistance screening agar base (ORSAB). The presence of MRSA was investigated in typical colonies growing on the three types of media. Discrepant results were resolved by detection of the mecA gene by polymerase chain reaction and the modified penicillin binding protein known as PBP2'. MRSA was de...
Bookmarks Related papers MentionsView impact
Virulence, 2014
Bookmarks Related papers MentionsView impact
Journal of Basic Microbiology, 2008
Bookmarks Related papers MentionsView impact
Jornal Vascular Brasileiro, 2008
Background: Surgical wound infection is a severe complication of peripheral vascular surgery. The... more Background: Surgical wound infection is a severe complication of peripheral vascular surgery. The recent appearance of resistant and aggressive pathogens brings new concerns related to the management of these infections.
Bookmarks Related papers MentionsView impact
Revista Da Sociedade Brasileira De Medicina Tropical, 2008
Coagulase-negative Staphylococcus has emerged as an important agent in nosocomial infections. In ... more Coagulase-negative Staphylococcus has emerged as an important agent in nosocomial infections. In this study, we report a case of bacteremia associated with a central venous catheter, caused by Staphylococcus cohnii spp urealyticus that was isolated in blood cultures from a 53-year-old male patient who was admitted to a general hospital in the city of São Paulo. We discuss in this report the difficulty in routinely identifying this microorganism in the clinical microbiology laboratory. Staphylococcus cohnii spp urealyticus is a microorganism found in human skin as part of the normal microbiota, and it can cause serious infections in humans, in some situations.
Bookmarks Related papers MentionsView impact
Revista da Sociedade Brasileira de Medicina Tropical, 2014
The genera Enterococcus, Staphylococcus and Streptococcus are recognized as important Gram-positi... more The genera Enterococcus, Staphylococcus and Streptococcus are recognized as important Gram-positive human pathogens. The aim of this study was to evaluate the performance of Vitek 2 in identifying Gram-positive cocci and their antimicrobial susceptibilities. One hundred four isolates were analyzed to determine the accuracy of the automated system for identifying the bacteria and their susceptibility to oxacillin and vancomycin. The system correctly identified 77.9% and 97.1% of the isolates at the species and genus levels, respectively. Additionally, 81.8% of the Vitek 2 results agreed with the known antimicrobial susceptibility profiles. Vitek 2 correctly identified the commonly isolated strains; however, the limitations of the method may lead to ambiguous findings.
Bookmarks Related papers MentionsView impact
Brazilian Journal of Infectious Diseases, 2007
Bookmarks Related papers MentionsView impact
In peripheral vascular surgery, cephalosporins are nowadays regarded as the first choice for oper... more In peripheral vascular surgery, cephalosporins are nowadays regarded as the first choice for operative antibiotic prophylaxis. We have recently observed changes in colonizing patterns, pathogen prevalence and antibiotic susceptibility to antimicrobials. Multiresistant pathogens are becoming more frequent in vascular surgical wound infections, showing regional and local variations as to prophylactic antibiotic susceptibility. Data from the available literature so far have
Bookmarks Related papers MentionsView impact
PLoS ONE, 2014
Bookmarks Related papers MentionsView impact
Jornal Brasileiro De Patologia E Medicina Laboratorial, 2004
Bookmarks Related papers MentionsView impact
Revista do Instituto de Medicina Tropical de São Paulo, 2014
Bookmarks Related papers MentionsView impact
Revista do Instituto de Medicina Tropical de São Paulo, 2006
In the present study, a total of 455 enterococcal isolates, recovered from patients living in the... more In the present study, a total of 455 enterococcal isolates, recovered from patients living in the city of Porto Alegre, State of Rio Grande do Sul, Brazil, during the period from July 1996 to June 1997, were identified to the species level by conventional biochemical and microbiological tests, and assayed for their susceptibilities to antimicrobial agents. The genetic diversity of antimicrobial resistant strains was evaluated by pulsed-field gel electrophoresis (PFGE) analysis of SmaI restricted chromosomal DNA. The most frequent species was Enterococcus faecalis (92.8%). Other species identified were: E. faecium (2.9%), E. gallinarum (1.5%), E. avium (1.1%), E. hirae (0.7%), E. casseliflavus (0.4%), E. durans (0.4%) and E. raffinosus (0.2%). The overall prevalence of isolates with high-level resistance (HLR) to aminoglycosides was 37.8%. HLR to gentamicin was found in 24.8%. No strains with acquired resistance to vancomycin were found. PFGE analysis showed the predominance of clonal group A, comprising strains isolated from different clinical specimens obtained from patients in three hospitals. These results suggest intra and inter-hospital dissemination of one predominant clonal group of E. faecalis isolates with HLR to gentamicin in the hospitals included in this study.
Bookmarks Related papers MentionsView impact
Brazilian Journal of Infectious Diseases, 2009
Enterococci are part of the endogenous flora of human beings, are naturally resistant to several ... more Enterococci are part of the endogenous flora of human beings, are naturally resistant to several classes of antimicrobials, and are able to acquire resistance with relative ease. Currently the vancomycin-resistant enterococci are spread all over the world and treatment options for infections caused by it are often extremely limited. We assessed 193 vancomycin-resistant Enterococcus faecalis isolates collected from four different hospitals in Porto Alegre for their susceptibility to fosfomycin using the E-test and agar diffusion. Fosfomycin proved to be active in vitro against the great majority of isolates, indicating that it is a valid option in the treatment of these infections.
Bookmarks Related papers MentionsView impact
Brazilian Journal of Infectious Diseases, 2007
Bookmarks Related papers MentionsView impact
Brazilian Journal of Infectious Diseases, 2006
Tuberculosis is an increasingly important public health problem in developing countries. We studi... more Tuberculosis is an increasingly important public health problem in developing countries. We studied 111 tuberculosis patients confirmed by mycobacteria isolation between 1998 and 2000 in Rio Grande, in south Brazil. One-hundred-thirteen pulmonary and extrapulmonary clinical materials were examined through culture by the Ogawa-Kudoh method and through smear examination by the Ziehl-Nielsen or Kinyoun methods. The identification of Mycobacterium tuberculosis was done through the usual phenotypical methods. The proportion method (indirect technique) was used to determine the resistance of isolates. The man/woman ratio was 2.6:1, 75% were white, and the group had a mean age of 39.7+/-12.7. Risk factors were present in 38.7% (34 men and 9 women, p=0.2), with a mean age of 35 (p=0.002); infection by HIV was the most frequent (28.8%), followed by alcoholism (16.2%) and injected drug use (15.3%). The frequency of M. tuberculosis isolates was 99.1%. Pulmonary disease occurred in 88.3% of the cases, extrapulmonary in 9.9% and 1.8% in both. There was an association between extrapulmonary tuberculosis and the presence of risk factors (p=0.0001). Resistance to isoniazid was found in 4% of the isolates and to isoniazid and rifampin in 2%, all being patients with some risk factors. The profile of tuberculosis in the population of this study followed the pattern described for developing regions of the world.
Bookmarks Related papers MentionsView impact
Revista da Sociedade Brasileira de Medicina Tropical, 2012
Bookmarks Related papers MentionsView impact
Uploads
Papers by Pedro D'Azevedo