Mar Larrosa
Universidad Europea de Madrid, Escuela de Doctorado e Investigación, Department Member
Regular physical exercise provides many health benefits, protecting against the development of chronic diseases, and improving quality of life. Some of the mechanisms by which exercise provides these effects are the promotion of an... more
Regular physical exercise provides many health benefits, protecting against the development of chronic diseases, and improving quality of life. Some of the mechanisms by which exercise provides these effects are the promotion of an anti-inflammatory state, reinforcement of the neuromuscular function, and activation of the hypothalamic-pituitary-adrenal (HPA) axis. Recently, it has been proposed that physical exercise is able to modify gut microbiota, and thus this could be another factor by which exercise promotes well-being, since gut microbiota appears to be closely related to health and disease. The purpose of this paper is to review the recent findings on gut microbiota modification by exercise, proposing several mechanisms by which physical exercise might cause changes in gut microbiota.
Research Interests: Organic Chemistry, Medicinal Chemistry, Cytokines, Diarrhea, Peroxidase, and 15 moreCell line, Prodrugs, Humans, Intestinal Mucosa, Mice, Animals, Colitis, Intestinal absorption, Medicinal, Feces, Stilbenes, Acute Phase Proteins, Colon, Structure activity Relationship, and Gastrointestinal Transit Time
... Mar Larrosa, Rafael Llorach, Juan Carlos Espín * and Francisco A. Tomás-Barberán. ... JA LARRAURI, I. GOÑI, N. MARTIN-CARRON, P. RUPEREZ and F. SAURA-CALIXTO, Measurement of health-promoting properties in fruit dietary fibres:... more
... Mar Larrosa, Rafael Llorach, Juan Carlos Espín * and Francisco A. Tomás-Barberán. ... JA LARRAURI, I. GOÑI, N. MARTIN-CARRON, P. RUPEREZ and F. SAURA-CALIXTO, Measurement of health-promoting properties in fruit dietary fibres: antioxidant capacity, fermentability and ...
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Research Interests: Nutrition and Dietetics, Food and Nutrition, Flavonoids, In Vitro, Models, and 20 morePolyphenols, Reactive Oxygen Species, Mice, Animals, Male, Bacteria, Anti-inflammatory agents, Colitis, Phenols, Rats, Animal models, Feces, Wistar Rats, Food Sciences, Faeces, Anti Inflammatory Activity, Colon, Polyphenol, Water Content, and Interleukin
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Research Interests: Engineering, Physics, Chemistry, Biology, Medicine, and 17 moreMultidisciplinary, Lipids, Animals, Lipid metabolism, Food Industry, PLoS one, Food intake, Plant extracts, Rats, Gastrointestinal Tract, Rosmarinus, Body Weight, Rat Model, Biological activity, Metabolic Disorder, Weight Gain, and Lipase
Research Interests: Obesity, Inflammation, Multidisciplinary, Female, Animals, and 7 morePLoS one, Plant extracts, Rats, Dietary fiber, Feces, Rosmarinus, and Body Weight
Research Interests: Transcriptomics, Cytokines, Inflammation, Transcriptome, Humans, and 18 morePolyphenols, Hypertension, Microarrays, ALP, Male, Dietary Supplements, MIR, Aged, Middle Aged, microRNAs, Plant extracts, Stilbenes, Pharmacological, Vitis, Coronary Artery Disease, Type 2 Diabetes Mellitus, CCL, and Gene Expression Regulation
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... Mar Larrosa, Rafael Llorach, Juan Carlos Espín * and Francisco A. Tomás-Barberán. ... JA LARRAURI, I. GOÑI, N. MARTIN-CARRON, P. RUPEREZ and F. SAURA-CALIXTO, Measurement of health-promoting properties in fruit dietary fibres:... more
... Mar Larrosa, Rafael Llorach, Juan Carlos Espín * and Francisco A. Tomás-Barberán. ... JA LARRAURI, I. GOÑI, N. MARTIN-CARRON, P. RUPEREZ and F. SAURA-CALIXTO, Measurement of health-promoting properties in fruit dietary fibres: antioxidant capacity, fermentability and ...
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... 9 Van het Hoff K H, de Boer B C, Tijburg L B, Lucious B R, Zijp I, West C E, Hautvast J G and Weststrate J A J, Carotenoid bioavailability in humans from tomatoes processed in different ways determined from the carotenoid response in... more
... 9 Van het Hoff K H, de Boer B C, Tijburg L B, Lucious B R, Zijp I, West C E, Hautvast J G and Weststrate J A J, Carotenoid bioavailability in humans from tomatoes processed in different ways determined from the carotenoid response in triglyceride-rich lipoprotein fraction of ...
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Research Interests: Nutrition and Dietetics, Cell Cycle, Gene expression, Inflammatory Bowel Disease, Nitric oxide, and 19 moreAnimals, Male, Tandem Mass Spectrometry, High Performance Liquid Chromatography, Colitis, Nutritional Biochemistry, Coumarins, Phenols, Plant extracts, Rats, Food Sciences, Rat Model, Base Sequence, Colon, Healthy Subjects, Prostaglandin E2, Biochemistry and cell biology, Gene Expression Regulation, and Inflammatory response
Research Interests: Nutrition and Dietetics, Flow Cytometry, Cell Cycle, Flavonoids, Gene expression, and 23 moreColorectal cancer, Humans, Polyphenols, Hydrogen Peroxide, Nutritional Biochemistry, Beverages, Caco-2 cells, Phenols, Tumor Suppressor Gene, Colon cancer, Cell Cycle regulation, Food Sciences, Cell Proliferation, Cell Cycle Arrest, Colon Carcinoma, Carcinoembryonic Antigen, Caco 2 Cell, Cell Survival, Pancreatin, Biochemistry and cell biology, Gene expression profiling, Cell Adhesion Molecules, and Cell Growth
Research Interests: Nutrition and Dietetics, Atherosclerosis, Cardiovascular disease, Gene expression, Molecular Mechanics, and 23 moreTranscriptome, Low Dose, Cholesterol, Female, Animals, Male, Hypercholesterolemia, Disease Prevention, The, Lipid metabolism, FABP, Nutritional Biochemistry, Microarray Analysis, Swine, Stilbenes, Food Sciences, Metabolic Disorder, Fatty Acid Binding Protein, Biochemistry and cell biology, Preventive measures, Down-Regulation, High fat diet, and Dietary fats
Research Interests: Nutrition and Dietetics, Obesity, Aging, Oxidative Stress, DNA damage, and 29 moreOlive Oil, Insulin Resistance, Antioxidants, Blood Pressure, Animals, Male, Superoxide Dismutase, Fatty Liver, Nutritional Biochemistry, Phenols, Rats, Time Factors, Rat, Oxidative Damage, Degeneration, Adipocytes, Fatty Acid, Age effect, Wistar Rats, Biological markers, Food Sciences, Long Term Effect, Long Term, Extra Virgin Olive Oil, Overall Survival, Blood cells, Plant Oils, Biochemistry and cell biology, and Random Allocation
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Research Interests: Organic Chemistry, Medicinal Chemistry, Cytokines, Diarrhea, Peroxidase, and 15 moreCell line, Prodrugs, Humans, Intestinal Mucosa, Mice, Animals, Colitis, Intestinal absorption, Medicinal, Feces, Stilbenes, Acute Phase Proteins, Colon, Structure activity Relationship, and Gastrointestinal Transit Time
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The effect of the naturally occurring polyphenol resveratrol... more
The effect of the naturally occurring polyphenol resveratrol (3,5,4'-trihydroxy-trans-stilbene; RES) on growth, cell cycle, and cyclins A, E, and B1 expression was investigated in the human SK-Mel-28 melanoma cell line. In addition, the structurally related compounds 4-hydroxy-trans-stilbene (4HST), piceatannol (3,5,3',4'-tetrahydroxy-trans-stilbene (PICE), and 4-trans-stilbenemethanol (4STMe) were also assayed in order to investigate the requirements of stilbenes to exert activity against melanoma cells. Both RES and 4HST inhibited cell growth in a dose- and time-dependent manner and upregulated the expression of cyclins A, E, and B1 with subsequent irreversible arrest of melanoma cells in the S-phase, concomitant with a decrease in G0/G1 and G2/M phases. In addition, potent apoptosis-mediated cell death was detected with the annexin V assay whereas no apoptosis was observed by flow cytometry, which encourages the assay of different methodologies to evaluate the effect of polyphenols on cell lines. The effect of PICE was not evaluated because of its instability in the reaction medium. No effect on cell cycle and cyclins expression was observed when 4STMe was assayed, which supported the critical requirement of the 4'-hydroxystyryl moiety to exert the above effects. In addition, this structural requirement also influenced the cellular uptake of stilbenes. The presence of two extra hydroxyl groups in RES increased its cytotoxicity whereas it diminished its efficiency to inhibit cell growth, upregulate cyclins expression, and arrest cell cycle in the S-phase with respect to 4HST. The present study suggests that the antimelanoma properties of dietary stilbenes, such as grape RES, cannot be ruled out, taking into account previous studies concerning the relationship between plasma and tissue concentrations and pharmacological activity of RES in animal models.
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Research Interests: Engineering, Inflammation, Cell line, Humans, Anti-inflammatory agents, and 12 moreCHEMICAL SCIENCES, Intestines, Coumarins, Plant extracts, Tumor necrosis factor-alpha, Biotransformation, Biological markers, Agricultural and Food Chemistry, Colon, Plasminogen Activator Inhibitor, Interleukin, and Ellagic acid
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A number of pharmacokinetic studies have shown marked differences in the plasma metabolic profile of resveratrol (RES) between humans and animals and between individuals of the same species, which complicates the identification of the... more
A number of pharmacokinetic studies have shown marked differences in the plasma metabolic profile of resveratrol (RES) between humans and animals and between individuals of the same species, which complicates the identification of the putative bioactive metabolites responsible for the beneficial effects of RES. On the basis of the physiological similarity between pigs and humans, the aim of this work was to characterize the metabolic profile and pharmacokinetics of RES in the plasma of pigs and to compare this to values reported in humans. RES (5.9 mg/kg of body weight) was orally administered to pigs. The following metabolites were identified in plasma using HPLC-MS/MS: RES-diglucuronide (1), two isomers of RES-sulfoglucuronide (2, 3), two isomers of RES-glucuronide (4, 5), RES-sulfate (6), and RES. The most abundant metabolites were 2, 5 (identified as resveratrol 3-O-glucuronide), and 6. The t(max) ranged from 0.9 h for compounds 2 and 5 to 2 h for compound 3. The highest C(max) value was 2223 ng/mL (5.5 μM) for metabolite 5, which was 2.6-, 3.3-, and 12-fold higher than that for metabolites 6, 2, and 3, respectively. Peak plasma levels of RES (53 ng/mL; 0.23 μM) were detected 0.5 h after RES ingestion. Apart from the low levels of RES aglycone, the RES metabolic profile in pigs differs from that found in humans. The identification of the actual active RES metabolites is a challenge that requires more complex studies which should take into account many possible influencing factors such as age, gender, and methodological approaches.