Laboratorio de Genética

Type 2 diabetes mellitus (T2D) is one of the two leading causes of mortality in Mexico. However, most studies have focused on Caucasians or Asians, and there are a small number of studies investigating Maya populations. Furthermore, to the best of our knowledge, there is no information on isolated Maya communities with T2D frequencies of 20% that are primarily attributed to ethnicity. Consequently, this study focused on assessing which genetic risk variants could be involved in the high rates of T2D in 92 individuals with Maya ancestry; 47 were diagnosed with T2D, and 45 were classified as healthy individuals. A pilot genome-wide association study was performed using the Affymetrix Axiom Genome-wide LAT1 array. The population structure was determined with the ADMIXTURE software using 1289 Latin American selected polymorphisms, and 39 polymorphisms associated with T2D were included for replication. Association tests were performed using the Statistical Analysis System (SAS) using the...

Introducción: La diabetes mellitus (DM) se ha convertido en una epidemia; en México las cifras ascienden a 12 millones de adultos. La debilidad muscular y pérdida de equilibrio que presentan los pacientes con diabetes mellitus tipo 2 (DT2) afecta el ciclo de la marcha lo que aumenta el riesgo de caídas (RC). Una de las estrategias que ha logrado reducir el RC en las personas que viven con DT2 son las intervenciones fisioterapéuticas. Objetivo: Determinar el efecto de una intervención fisioterapéutica en la marcha, sobre el puntaje de RC (PRC) en adultos con DT2. Material y Métodos: Estudio prospectivo, longitudinal, cuasi experimental. Se realizó una intervención fisioterapéutica en la marcha a adultos con DT2, que incluyó ejercicios de fortalecimiento, estiramientos, de equilibrio y coordinación; se calcularon los rangos de movimiento articular (RDM) fuerza muscular (FM) (escala de Daniels-Worthingham), huella plantar mediante el índice de Hernández-Corvo (IHC) de los miembros infe...

It has been demonstrated that heterozygote and homozygote thiopurine S-methyltransferase (TPMT) mutant allele carriers are at high risk to develop severe and potentially fatal hematopoietic toxicity after treatment with standard doses of 6-mercaptopurine (6-MP) and methotrexate (MX). Those drugs are the backbone of acute lymphoblastic leukemia (ALL) and several autoimmune disease treatments. We undertook this study to determine the frequency of the TPMT deficient alleles in children with ALL and non-ALL subjects from Mexico City and Yucatan, Mexico. We included 849 unrelated subjects, of which 368 ALL children and 342 non-ALL subjects were from Mexico City, and 60 ALL cases and 79 non-ALL individuals were from Yucatan. Genotyping of the rs1800462, rs1800460 and rs1142345 SNPs was performed by 5'exonuclease technique using TaqMan probes (Life Technologies Foster City, CA). The mutant TPMT alleles were present in 4.8% (81/1698 chromosomes) and only 0.2% were homozygote TPMT*3A/TPM...