- Shenzhen, China
- +8615012645916
Tesfaye Wolde
Tsinghua University, Precision Medicine and Healthcare, Graduate Student
This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
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This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY
Research Interests:
Antibiotic prescription is the first line of treating bacterial diseases. But it affects the composition and function of non-targeted intestinal microbiota. Antimicrobial resistance will be the cause for the death of millions of people... more
Antibiotic prescription is the first line of treating bacterial diseases. But it affects the composition and function of non-targeted intestinal microbiota. Antimicrobial resistance will be the cause for the death of millions of people worldwide. By considering collateral damage of antibiotics, searching an alternative antimicrobial agent will be a solution to minimize the problems. Probiotics, prebiotics and synbiotics are an alternative to antibiotics. Thanks to GIT microbes as they are responsible for metabolism, energy production, protection from pathogens and immunomodulation. So, it is appreciable if the gut microbiota modulated through consumption of probiotics, prebiotics and synbiotics. Probiotic effects are genus and species specific.
To get good health benefit of probiotics, safety of probiotics should be assessed before using them. This is mandatory for prebiotics and synbiotic selection. So this review paper gives information about side effects of antibiotics on gut microbiota, description of probiotics, prebiotics and synbiotics as alternative to antibiotics in
modulating GIT microbiota, techniques of assessing safety of probiotics and health benefits of probiotics, prebiotics and synbiotics with scientific evidence in clinical trial and some disagreement and challenges of using probiotics, prebiotics and synbiotics.
To get good health benefit of probiotics, safety of probiotics should be assessed before using them. This is mandatory for prebiotics and synbiotic selection. So this review paper gives information about side effects of antibiotics on gut microbiota, description of probiotics, prebiotics and synbiotics as alternative to antibiotics in
modulating GIT microbiota, techniques of assessing safety of probiotics and health benefits of probiotics, prebiotics and synbiotics with scientific evidence in clinical trial and some disagreement and challenges of using probiotics, prebiotics and synbiotics.
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Transfusion-transmissible infectious agents such as human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV), and Syphilis are among the greatest threats to blood safety for recipients. They are also the... more
Transfusion-transmissible infectious agents such as human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV), and Syphilis are among the greatest threats to blood safety for recipients. They are also the leading causes of death and chronic and life-threatening abnormalities. A retrospective analysis of consecutive blood donors' records covering the period between January 2010 and December 2014 was conducted to analyze for seroprevalence of HIV, HBV, HCV and syphilis among blood donors aged 17-65 years. The association of these infections with age group, blood group, their co-infection rate and year trends were analyzed. Linear regression analysis was used to determine trends of HIV, HBV, HCV and syphilis infections. Sterile venous anti-coagulated blood was collected from the donors and analyzed using highly sensitive and specific kits. From the total of 6827 consecutive blood donors, 963(14.1%) had serological evidence of infection with at least one p...
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Research Interests: Bioinformatics, Psychiatry, Immunology, Research Methodology, Life Sciences, and 15 moreDeveloping Countries, Health, Medicine, Screening, India, Prospective studies, Pregnancy, Humans, Biomedical Research, Measurement, Prevalence, Blood Donors, Seroprevalence, Medical and Health Sciences, and HIV infections
Staphylococcal poisoning is caused by ingestion of foods containing enterotoxins produced by some species of staphylococci. It is characterized by sudden onset of symptoms, including nausea, vomiting, abdominal cramps, and diarrhoea... more
Staphylococcal poisoning is caused by ingestion of foods containing enterotoxins produced by some species of staphylococci. It is characterized by sudden onset of symptoms, including nausea, vomiting, abdominal cramps, and diarrhoea within 1to 6 hr after ingestion of toxin-contaminated foods. The aim of this study was to determine prevalence and antibiotics resistance patterns of S. aureus isolates among food handlers. The study was conducted from March to May 2014. All food handlers in Jigjiga University students’ cafeteria regardless of their age, religion were the study population; 300 volunteer individuals were selected randomly. Mannitol Salt Agar, and Nutrient Broth supplemented with 7.5% (w/v) sodium chloride were used for culturing. Among these food handlers none of them had got food safety training. Educational status of the study participants was mainly 160 (53.3%) illiterate and primary school level. From a total of 300 individuals examined 160 (53.3%) food handler’s hand...
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A cross-sectional study was carried out to investigate the prevalence and antibiotic resistance patterns of Staphylococcus aureus isolated from kitchen sponges used in food establishments of Jimma town, between October, 2010 and June,... more
A cross-sectional study was carried out to investigate the prevalence and antibiotic resistance patterns of Staphylococcus aureus isolated from kitchen sponges used in food establishments of Jimma town, between October, 2010 and June, 2011. A total of 201 kitchen sponge samples from 20 restaurants, 101 hotels, 47 cafeterias and 33 pastry shops were enrolled in this study. Antibiotics susceptibility patterns of S. aureus isolates were done using 12 selected antibiotics. Out of 201 samples examined 69(34.3%) kitchen sponges were found to have S. aureus. Isolation rates of S. aureus differed among the food establishment types and it ranged from 30% (restaurants) to 36.4% (hotels). Significant variation in prevalence of S. aureus among kitchen sponges of restaurants, hotels, pastry shops and cafeterias were revealed (p=0.034). Ampicillin and Streptomycin were the most resisted drugs. Norfloxacin, Amikacin and Ciprofloxacin showed maximum sensitivity. Nine (9) drug resistance patterns we...
A cross-sectional study was carried out in Somali Regional State of Ethiopia to investigate the hygienic milk handling practices, bacterial loads across different sampling points in the market chain from March 2013 to July 2014. The study... more
A cross-sectional study was carried out in Somali Regional State of Ethiopia to investigate the hygienic milk handling practices, bacterial loads across different sampling points in the market chain from March 2013 to July 2014. The study employed questionnaire survey and bacteriological load analysis. One hundred and two (102) randomly selected milk producing households were interviewed for the survey-based study. A total of 120 [each 30 from udder, milking bucket, storage containers and market points] raw milk samples were also aseptically collected and tested for bacteriological load analysis. The overall mean plate count of aerobic mesophilic bacteria were 5.401log10cfu/ ml from udder, 8.132log10cfu/ ml from milking bucket, 9.976log10cfu/ml from storage containers and 12.699log10cfu/ml from market. There was an obviously steady increasing trend of plate counts throughout the value chain (P<0.05). The results of the current study indicated that the cow milk produced and distri...
Research Interests: Pharmacology and Pharmacy
Kitchen sponges are among the possible sources of contaminants in food establishments. The main purpose of the current study was, therefore, to assess the microbiological safety of sponges as it has been used in selected food... more
Kitchen sponges are among the possible sources of contaminants in food establishments. The main purpose of the current study was, therefore, to assess the microbiological safety of sponges as it has been used in selected food establishments of Jimma town. Accordingly, the microbiological safety of a total of 201 kitchen sponges randomly collected from food establishments was evaluated against the total counts of aerobic mesophilic bacteria (AMB), Enterobacteriaceae, coliforms, and yeast and molds. The mean counts of aerobic mesophilic bacteria ranged from 7.43 to 12.44 log CFU/mm(3). The isolated genera were dominated by Pseudomonas (16.9%), Bacillus (11.1%), Micrococcus (10.6%), Streptococcus (7.8%), and Lactobacillus (6%) excluding the unidentified Gram positive rods (4.9%) and Gram negative rods (9.9%). The high microbial counts (aerobic mesophilic bacteria, coliforms, Enterobacteriaceae, and yeast and molds) reveal the existence of poor kitchen sponge sanitization practice. Awar...
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A miniature refinery, the liver plays a major role in metabolic processes, performing a number of physiological functions. Nevertheless, the liver's susceptibility to damage is far greater than that of any other internal organ (Owais... more
A miniature refinery, the liver plays a major role in metabolic processes, performing a number of physiological functions. Nevertheless, the liver's susceptibility to damage is far greater than that of any other internal organ (Owais et al., 2006). Perpetual exposure of the liver to xenobiotics and ther-apeutic agents leads to toxic manifestations of a complex and diverse nature. Liver diseases are one of the major causes of morbidity and mortality, affecting humans of all ages. Published reports indicate that globally, millions of people are either acutely or chronically infected with hepatitis A, B, and C viruses. And out of these, an estimated 600,000 persons die each year due to acute or chronic consequences of hepatitis B and 25% of adults who become chronic-ally infected during childhood later die from liver cancer or cirrhosis (Liaw and Chu, 2009). Thus, the impact of liver disorders on the over-all population around the globe is considerable. Not a single curative therap...
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A cross-sectional study was carried out to investigate the prevalence and antibiotic resistance patterns of Staphylococcus aureus isolated from kitchen sponges used in food establishments of Jimma town, between October, 2010 and June,... more
A cross-sectional study was carried out to investigate the prevalence and antibiotic resistance patterns of Staphylococcus aureus isolated from kitchen sponges used in food establishments of Jimma town, between October, 2010 and June, 2011. A total of 201 kitchen sponge samples from 20 restaurants, 101 hotels, 47 cafeterias and 33 pastry shops were enrolled in this study. Antibiotics susceptibility patterns of S. aureus isolates were done using 12 selected antibiotics. Out of 201 samples examined 69(34.3%) kitchen sponges were found to have S. aureus. Isolation rates of S. aureus differed among the food establishment types and it ranged from 30% (restaurants) to 36.4% (hotels). Significant variation in prevalence of S. aureus among kitchen sponges of restaurants, hotels, pastry shops and cafeterias were revealed (p=0.034). Ampicillin and Streptomycin were the most resisted drugs. Norfloxacin, Amikacin and Ciprofloxacin showed maximum sensitivity. Nine (9) drug resistance patterns were detected among S. aureus isolates. There was significant variation in the prevalence of Staphylococcus aureus. Kitchen sponges used in food service establishments of Jimma town recognized as potential agents in the spread of microorganisms, and the isolates showed high resistant patterns to Ampicillin and Streptomycin.