Papers by Zsuzsanna Bosze
Differentiation, 2011
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Cell Biology International Reports, 1990
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Biochemical and Biophysical Research Communications, 1999
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Small Ruminant Res, 2007
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Scientific Reports, 2015
In drug discovery, prediction of selectivity and toxicity require the evaluation of cellular calc... more In drug discovery, prediction of selectivity and toxicity require the evaluation of cellular calcium homeostasis. The rat is a preferred laboratory animal for pharmacology and toxicology studies, while currently no calcium indicator protein expressing rat model is available. We established a transgenic rat strain stably expressing the GCaMP2 fluorescent calcium sensor by a transposon-based methodology. Zygotes were co-injected with mRNA of transposase and a CAG-GCaMP2 expressing construct, and animals with one transgene copy were pre-selected by measuring fluorescence in blood cells. A homozygous rat strain was generated with high sensor protein expression in the heart, kidney, liver, and blood cells. No pathological alterations were found in these animals, and fluorescence measurements in cardiac tissue slices and primary cultures demonstrated the applicability of this system for studying calcium signaling. We show here that the GCaMP2 expressing rat cardiomyocytes allow the prediction of cardiotoxic drug side-effects, and provide evidence for the role of Na(+)/Ca(2+) exchanger and its beneficial pharmacological modulation in cardiac reperfusion. Our data indicate that drug-induced alterations and pathological processes can be followed by using this rat model, suggesting that transgenic rats expressing a calcium-sensitive protein provide a valuable system for pharmacological and toxicological studies.
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Journal of Virology
The transcriptional enhancers of the Moloney murine sarcoma virus (MuSV) and Moloney murine leuke... more The transcriptional enhancers of the Moloney murine sarcoma virus (MuSV) and Moloney murine leukemia virus (MuLV) have different cell type specificities from that of the Friend MuLV. While the three enhancers are approximately equally active in erythroid cells, the Moloney MuSV and Moloney MuLV enhancers are 20- to 40-fold more active than the Friend MuLV enhancer in T-lymphoid cells. Using mutant enhancers, we have shown that specific differences between the nucleotide sequences of the Moloney MuSV and Friend MuLV enhancers are responsible for their different activities in T cells. Our data allow the localization of a DNA element, repeated several times within the enhancer, which modulates the activity of the enhancer in T cells without affecting it in erythroid cells. This element therefore appears to be one of the determinants of the tissue specificity of the enhancer.
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Transgenic research, 2003
Until recently, transgenic rabbits were produced exclusively by pronuclear microinjection which r... more Until recently, transgenic rabbits were produced exclusively by pronuclear microinjection which results in additive random insertional transgenesis; however, progress in somatic cell cloning based on nuclear transfer will soon make it possible to produce rabbits with modifications to specific genes by the combination of homologous recombination and subsequent prescreening of nuclear donor cells. Transgenic rabbits have been found to be excellent animal models for inherited and acquired human diseases including hypertrophic cardiomyopathy, perturbed lipoprotein metabolism and atherosclerosis. Transgenic rabbits have also proved to be suitable bioreactors for the production of recombinant protein both on an experimental and a commercial scale. This review summarizes recent research based on the transgenic rabbit model.
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The EMBO journal, 1986
We investigated the ability of the U3 region of the long terminal repeats (LTR) of the Friend mur... more We investigated the ability of the U3 region of the long terminal repeats (LTR) of the Friend murine leukemia virus (Fr-MuLV) and Moloney murine leukemia virus (Mo-MuLV) to promote transcription in a variety of human cell lines. Our analysis reveals the presence of a transcriptional enhancer with specificity for erythroid cells in the U3 region of the Fr-MuLV. This constitutes the first example of an enhancer with such a property. Analysis of the Mo-MuLV enhancer suggests that it is active at least in erythroid and lymphoid cells and has thus a less restricted specificity than the Fr-MuLV enhancer. The different tissue specificities of the two enhancers correlate with the different tissue selectivities and pathogenic properties of the two viruses.
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Cell Biology International Reports, 1990
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Transgenic Research, 2007
We generated and characterized transgenic mice carrying a 102 kb bovine genomic fragment, encodin... more We generated and characterized transgenic mice carrying a 102 kb bovine genomic fragment, encoding the neonatal Fc receptor alpha-chain (bFcRn). FcRn plays a crucial role in the maternal IgG transport and it also regulates the IgG and albumin homeostasis. Some of its functions and transcriptional regulation show species specific differences. The FcRn heterodimer is composed of the alpha-chain and beta-2-microglobulin (beta2 m). A bacterial artificial chromosome containing the bovine FcRn alpha-chain gene (bFCGRT) with its 44 kb 5' and 50 kb long 3' flanking sequences was microinjected into fertilized mouse oocytes. Two of the transgenic lines generated, showed copy number related and integration site independent bFcRn expression. The bFcRn alpha-chain forms a functional receptor with the mouse beta2-microglobulin and extends the half-life of the mouse IgG in transgenic mice. Our results underline the feasibility of creating BAC transgenic mouse models of economically important bovine genes.
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Small Ruminant Research, 2007
The aim of this study was to perform an initial characterization of milk quality and to determine... more The aim of this study was to perform an initial characterization of milk quality and to determine genetic polymorphism at the CSN1S1 and CSN1S2 locus in two herds of local dairy goats (Hungarian Milking). The fat, protein and lactose level of milk samples in Hungarian Milking Goats were compared to other local goat breeds worldwide and it was concluded that
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Livestock Production Science, 1997
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The Journal of Immunology, 2011
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Small Ruminant …, 2008
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Journal of the American Society of Nephrology, 2015
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Journal of the American Society of Nephrology, 2015
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Cloning and Stem Cells, 2007
The aim of this study was to develop a method to generate identical twins and triplets with predi... more The aim of this study was to develop a method to generate identical twins and triplets with predicted gender. As a first step toward that aim, single blastomeres obtained from EGFP expressing eight-cell stage embryos and either diploid or tetraploid host embryos were used to compose chimera. We could follow the fate of EGFP expressing diploid blastomere derived cells in 3.5- and 4.5-day-old chimera embryos in vitro. We found that the diploid blastomere-derived cells had significantly higher chance to contribute to the inner cell mass if tetraploid host embryos were applied. After that, we developed a quick and reliable multiplex PCR strategy for sex diagnosis from single blastomeres by simultaneous amplification of the homologous ZFX and ZFY genes. By composed chimeras using single blastomeres, derived from sexed eight-cell stage embryos and a tetraploid host embryo, we could get preplanned sex newborns, wholly derived from these blastomeres. Among these mice, identical twins and a triplet were identified by microsatellite analysis. Unlike clones produced by nuclear transfer, these mice are identical at both the nuclear as well as mitochondrial DNA level. Therefore, the tetraploid embryo complementation method to produce monozygotic twins and triplets could be a valuable tool both in biomedical and agricultural applications.
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Transgenic Research, 2006
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Papers by Zsuzsanna Bosze