Asian Pacific journal of cancer prevention : APJCP, 2016
E-cadherin (CDH1) genetic variations alter gene transcriptional activity of epithelial cells in v... more E-cadherin (CDH1) genetic variations alter gene transcriptional activity of epithelial cells in vitro and may cause susceptibility to various cancers. Associations of CDH1 -160C>A polymorphism with various cancers have been widely reported. However, the results are controversial and inconsistent. To derive a more accurate estimation of the relationship, a meta-analysis was performed with regard to gastrointestinal (GI) cancer risk. Eligible studies were identified through a search of PubMed database until December 2015. Associations between the CDH1 -160C>A polymorphism and GI cancer risk was considered by odds ratios (ORs) together with their 95% confidence intervals (CIs). A total of 31 studies including 11,606 cases and 12,655 controls were involved in this meta-analysis. Overall, this meta-analysis showed no association between CDH1 -160C>A polymorphism and GI cancer risk (A vs. C: OR = 1.08, 95%CI = 0.98-1.18, P = 0.086;CA vs. CC: OR = 1.09, 95%CI = 0.97- 1.22, P = 0.1...
The Journal of Maternal-Fetal & Neonatal Medicine, 2016
Process of angiogenesis is essential for successful gestation. Disruption in this pathway leads t... more Process of angiogenesis is essential for successful gestation. Disruption in this pathway leads to various undesirable consequences in pregnancy such as recurrent spontaneous abortion (RSA). One of the most important genes involved in angiogenesis is kinase-insert domain-containing receptor (KDR). This study aimed to investigate the associations between two single-nucleotide polymorphisms (SNPs) of KDR gene, 1719A > T and 1192G > A, with idiopathic RSA in south-east Iran. A total of 230 women, including 110 women with a history of at least two consecutive spontaneous miscarriages and 120 healthy women were recruited in this study. Genomic DNA was extracted from peripheral blood samples of participants using the Salting out method. The KDR 1719A > T and 1192G > A polymorphisms were genotyped by the standard amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) technique. For the case group, frequencies of 2.73%, 30% and 67.27% were observed for AA, AT and TT genotypes in1719A > T SNP, respectively, and the genotype frequencies for controls were equal to AA = 3.33%, AT = 32.5% and TT = 64.17%. Distribution of genotypes in 1192G > A SNP in the case group was 79.1%, 19.1% and 1.8% for GG, AG and AA, respectively, whereas the corresponding values for the controls were GG = 80%, AG = 20% and AA = 0. No significant difference was found between the case and control groups based on the frequency of KDR gene polymorphisms with the susceptibility to RSA. There is no association between these two SNPs of KDR gene and the susceptibility to RSA in women from south-east Iran.
Graefe's Archive for Clinical and Experimental Ophthalmology, 2015
This study aims at investigating possible associations of P-selectin polymorphisms with prolifera... more This study aims at investigating possible associations of P-selectin polymorphisms with proliferative diabetic retinopathy (PDR) in Yazd, Iran. The subjects of the study included of 55 PDR and 55 diabetic no retinopathy (DNR) cases attending Yazd Diabetes Research Center (YDRC). P-selectin genotyping was done by an ARMS-PCR method. The P-selectin variants rs6128, rs6133, and rs3917779 were not in Hardy-Weinberg equilibrium. The frequency of the rs3917779 C allele (P < 0.0001), but not the rs6133 G allele (P = 0.19) or rs6128 allele (P = 0.20), was higher in PDR cases than in control DNR cases. Significant differences in the distribution of rs3917779 (P < 0.001), but not rs6128 (P = 0.52) or rs6133 (P = 0.18), genotypes were observed between cases and controls, and only rs3917779 showed a significant association with PDR, with increments of 49.2 (14.72-125.07) in disease risk seen for CC genotypes. Among the eight three-locus P-selectin haplotypes constructed (rs6128 / rs6133 / rs3917779), there was no significant difference between frequencies of haplotypes in the DNR and PDR groups. P-selectin gene polymorphisms and haplotypes can contribute to PDR development.
Sulfatase 1 (SULF1) function is to remove the 6-O-sulphate group from heparan sulfate. This actio... more Sulfatase 1 (SULF1) function is to remove the 6-O-sulphate group from heparan sulfate. This action changes the binding sites of extracellular growth factors. SULF1 expression has been reported to be changed in angiogenesis. We hypothesized that single nucleotide polymorphisms (SNPs) of SULF1 would impact clinicopathologic characteristics. Study of SULF1 gene polymorphism with fetus failure in in vitro fertilization (IVF) technique. We studied one common (minor allele frequency >0.05) regulatory SNP, rs6990375, with polymerase chain reaction and restriction fragment length polymorphism method, in 53 infertile women with fetus failure in IVF technique and 53 women with at least one healthy child as controls. We found that rs6990375 is significantly associated with an early failure in IVF and frequency of G allele is high in women with fetus failure in IVF technique (p<0.001). These findings suggest that SULF1genetic variations may play a role in IVF technique fetus failure. Furt...
Resistance to chemotherapy in lung adenocarcinoma remains a major obstacle. We examined the poten... more Resistance to chemotherapy in lung adenocarcinoma remains a major obstacle. We examined the potential role of Octamer-binding transcription factor-4B (OCT4B) in enhancing sensitivity of lung adenocarcinoma cells to cisplatin. RNAi interference was used to examine the role of OCT4B in cisplatin-treated A549 cells. Cells were transfected with OCT4B siRNA prior to a 48-h cisplatin treatment. Propidium iodide (PI) and caspase-3 staining were used to determine cell viability and apoptosis. Cell-cycle analysis was performed to evaluate alterations in phase distribution. OCT4B suppression in cells increased the number of non-viable, PI(+), and apoptotic, caspase-3(+) cells in the presence and absence of cisplatin treatment. Importantly, cisplatin treatment of OCT4B-suppressed cells resulted in a marked transition of cells from G0/G1 to G2/M phase. Silencing of OCT4B confers sensitivity to cisplatin treatment in A549 cells via cell-cycle regulation, increased proliferation and enhancement o...
Objective: OCT4 is a master regulator of self-renewal and involved in regulating pluripotency in ... more Objective: OCT4 is a master regulator of self-renewal and involved in regulating pluripotency in embryonic stem cells (ESCs). In addition to the previously described spliced variants of the gene (eg OCT4A and OCT4B), we have recently identified a novel variant of the gene, ...
Asian Pacific journal of cancer prevention : APJCP, 2016
E-cadherin (CDH1) genetic variations alter gene transcriptional activity of epithelial cells in v... more E-cadherin (CDH1) genetic variations alter gene transcriptional activity of epithelial cells in vitro and may cause susceptibility to various cancers. Associations of CDH1 -160C>A polymorphism with various cancers have been widely reported. However, the results are controversial and inconsistent. To derive a more accurate estimation of the relationship, a meta-analysis was performed with regard to gastrointestinal (GI) cancer risk. Eligible studies were identified through a search of PubMed database until December 2015. Associations between the CDH1 -160C>A polymorphism and GI cancer risk was considered by odds ratios (ORs) together with their 95% confidence intervals (CIs). A total of 31 studies including 11,606 cases and 12,655 controls were involved in this meta-analysis. Overall, this meta-analysis showed no association between CDH1 -160C>A polymorphism and GI cancer risk (A vs. C: OR = 1.08, 95%CI = 0.98-1.18, P = 0.086;CA vs. CC: OR = 1.09, 95%CI = 0.97- 1.22, P = 0.1...
The Journal of Maternal-Fetal & Neonatal Medicine, 2016
Process of angiogenesis is essential for successful gestation. Disruption in this pathway leads t... more Process of angiogenesis is essential for successful gestation. Disruption in this pathway leads to various undesirable consequences in pregnancy such as recurrent spontaneous abortion (RSA). One of the most important genes involved in angiogenesis is kinase-insert domain-containing receptor (KDR). This study aimed to investigate the associations between two single-nucleotide polymorphisms (SNPs) of KDR gene, 1719A &amp;amp;amp;amp;gt; T and 1192G &amp;amp;amp;amp;gt; A, with idiopathic RSA in south-east Iran. A total of 230 women, including 110 women with a history of at least two consecutive spontaneous miscarriages and 120 healthy women were recruited in this study. Genomic DNA was extracted from peripheral blood samples of participants using the Salting out method. The KDR 1719A &amp;amp;amp;amp;gt; T and 1192G &amp;amp;amp;amp;gt; A polymorphisms were genotyped by the standard amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) technique. For the case group, frequencies of 2.73%, 30% and 67.27% were observed for AA, AT and TT genotypes in1719A &amp;amp;amp;amp;gt; T SNP, respectively, and the genotype frequencies for controls were equal to AA = 3.33%, AT = 32.5% and TT = 64.17%. Distribution of genotypes in 1192G &amp;amp;amp;amp;gt; A SNP in the case group was 79.1%, 19.1% and 1.8% for GG, AG and AA, respectively, whereas the corresponding values for the controls were GG = 80%, AG = 20% and AA = 0. No significant difference was found between the case and control groups based on the frequency of KDR gene polymorphisms with the susceptibility to RSA. There is no association between these two SNPs of KDR gene and the susceptibility to RSA in women from south-east Iran.
Graefe's Archive for Clinical and Experimental Ophthalmology, 2015
This study aims at investigating possible associations of P-selectin polymorphisms with prolifera... more This study aims at investigating possible associations of P-selectin polymorphisms with proliferative diabetic retinopathy (PDR) in Yazd, Iran. The subjects of the study included of 55 PDR and 55 diabetic no retinopathy (DNR) cases attending Yazd Diabetes Research Center (YDRC). P-selectin genotyping was done by an ARMS-PCR method. The P-selectin variants rs6128, rs6133, and rs3917779 were not in Hardy-Weinberg equilibrium. The frequency of the rs3917779 C allele (P &amp;amp;lt; 0.0001), but not the rs6133 G allele (P = 0.19) or rs6128 allele (P = 0.20), was higher in PDR cases than in control DNR cases. Significant differences in the distribution of rs3917779 (P &amp;amp;lt; 0.001), but not rs6128 (P = 0.52) or rs6133 (P = 0.18), genotypes were observed between cases and controls, and only rs3917779 showed a significant association with PDR, with increments of 49.2 (14.72-125.07) in disease risk seen for CC genotypes. Among the eight three-locus P-selectin haplotypes constructed (rs6128 / rs6133 / rs3917779), there was no significant difference between frequencies of haplotypes in the DNR and PDR groups. P-selectin gene polymorphisms and haplotypes can contribute to PDR development.
Sulfatase 1 (SULF1) function is to remove the 6-O-sulphate group from heparan sulfate. This actio... more Sulfatase 1 (SULF1) function is to remove the 6-O-sulphate group from heparan sulfate. This action changes the binding sites of extracellular growth factors. SULF1 expression has been reported to be changed in angiogenesis. We hypothesized that single nucleotide polymorphisms (SNPs) of SULF1 would impact clinicopathologic characteristics. Study of SULF1 gene polymorphism with fetus failure in in vitro fertilization (IVF) technique. We studied one common (minor allele frequency >0.05) regulatory SNP, rs6990375, with polymerase chain reaction and restriction fragment length polymorphism method, in 53 infertile women with fetus failure in IVF technique and 53 women with at least one healthy child as controls. We found that rs6990375 is significantly associated with an early failure in IVF and frequency of G allele is high in women with fetus failure in IVF technique (p<0.001). These findings suggest that SULF1genetic variations may play a role in IVF technique fetus failure. Furt...
Resistance to chemotherapy in lung adenocarcinoma remains a major obstacle. We examined the poten... more Resistance to chemotherapy in lung adenocarcinoma remains a major obstacle. We examined the potential role of Octamer-binding transcription factor-4B (OCT4B) in enhancing sensitivity of lung adenocarcinoma cells to cisplatin. RNAi interference was used to examine the role of OCT4B in cisplatin-treated A549 cells. Cells were transfected with OCT4B siRNA prior to a 48-h cisplatin treatment. Propidium iodide (PI) and caspase-3 staining were used to determine cell viability and apoptosis. Cell-cycle analysis was performed to evaluate alterations in phase distribution. OCT4B suppression in cells increased the number of non-viable, PI(+), and apoptotic, caspase-3(+) cells in the presence and absence of cisplatin treatment. Importantly, cisplatin treatment of OCT4B-suppressed cells resulted in a marked transition of cells from G0/G1 to G2/M phase. Silencing of OCT4B confers sensitivity to cisplatin treatment in A549 cells via cell-cycle regulation, increased proliferation and enhancement o...
Objective: OCT4 is a master regulator of self-renewal and involved in regulating pluripotency in ... more Objective: OCT4 is a master regulator of self-renewal and involved in regulating pluripotency in embryonic stem cells (ESCs). In addition to the previously described spliced variants of the gene (eg OCT4A and OCT4B), we have recently identified a novel variant of the gene, ...
Uploads
Papers