A novel UPLC method for determination of chlorhexidine gluconate and lidocaine hydrochloride in t... more A novel UPLC method for determination of chlorhexidine gluconate and lidocaine hydrochloride in throat sprays has been presented as a tool suitable for quality monitoring of these compounds. The developed and validated UPLC method was compared with HPLC method. Experimental parameters like analysis time, sensitivity, mobile phase consumption, validation data, and demands on instrument equipment and operation were compared for two methods. UPLC and HPLC are important methods for routine monitoring of these drugs. In UPLC, Major degradation was found in photolytic condition that product was degraded up to 30 %. The major impurity peaks was found at 3.73 min. The concentration range was varied between 0.040-0.160 mg/mL, 0.016-0.064 mg/mL for chlorhexidine gluconate and lidocaine hydrochloride respectively. The response of the drug was found to be linear in the investigation concentration range and the linear regression equation for both the technique HPLC and UPLC fitted well. The RSD ...
ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was e... more ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
International Letters of Chemistry, Physics and Astronomy, 2014
Simple and rapid reverse phase high-performance liquid chromatography (RP-HPLC) method was develo... more Simple and rapid reverse phase high-performance liquid chromatography (RP-HPLC) method was developed and validated using Phenomenex Gemini c18 (4.6 x 250 mm, 5 µ) reverse phase column for the determination of LAF in human plasma, Solid Phase Extraction (SPE) technique was used for the extraction of analyte, detection was carried out by Photo Diode Array detector at 216 nm. Chromatographic resolution of the LAF was achieved within 4.6 min by using mobile phase Methanol and 5 mM Di-Potassium Hydrogen Phosphate Buffer (pH 9.5) (80:20, v/v), flow rate was 1.0 mL/min. Calibration curve was linear with correlation coefficient of 0.9996 in the range of 50-1000 ng/mL, Limit of Detection (LOD) and Limit of Quantitation (LOQ) were 10 ng/mL and 30 ng/mL respectively, intra and inter-day deviations were lower than 3.92% and 3.98% respectively. The overall mean recovery of LAF was 94.57%. No any endogenous constituents were found to interfere at retention time of the analyte. This new RP-HPLC me...
A new generation of chroman bearing heterocyclic five membered ring such as 1,2,4-triazoles and t... more A new generation of chroman bearing heterocyclic five membered ring such as 1,2,4-triazoles and thiazolidinones was designed and synthesized. New chroman based nucleus 5-(6-fluorochroman-2-yl)-4-aryl-4H-1,2,4-triazole-3-thiol and 6-fluorochroman-N-(4-oxo-2-arylthiazolidinin-3-yl) chroman-2-carboxamides were synthesized. Aryl triazole compounds4a–4jwere synthesized from 6-fluorochroman-2-carbohydrazide2on reaction with base in methanol and CS2followed by reaction with substituted aniline. Thiazolidinone compounds5a–5jwere synthesized from 6-fluorochroman-2-carbohydrazide2on reaction with substituted aryl aldehyde and thioglycolic acid.
Journal of Liquid Chromatography & Related Technologies, 2011
... R. Patel a , Hitendra S. Joshi b & Atul H. Bapodra c pages 1699-1711. ... Rane , ... more ... R. Patel a , Hitendra S. Joshi b & Atul H. Bapodra c pages 1699-1711. ... Rane , VP ; Shinde , DB Development and Validation of Chiral LC Method for the Enantiomeric Separation of Duloxetine on Amylose Based Stationary Phase . J. Chromatogr. Sci. 2008 , 46 , 772 776 . ...
The objective of the current study was to develop a simple, precise and accurate High Perfomance ... more The objective of the current study was to develop a simple, precise and accurate High Perfomance Thin Layer Chromatographic [HPTLC] assay method and validated for determination of furosemide and spironolactone in solid pharmaceutical dosage forms. The mobile phase comprising of ethyl acetate: haxane in the volume ratio of [80: 20, v/v] was employed for the elution. Standard solution was prepared in methanol. The standard concentration was 40 µg ml -1 of furosemide and 100 µg ml -1 of spironolactone. Chromatographic analysis was performed on a HPTLC plates precoated with 0.25 mm layer of chromatographic silica gel mixture [Silica GF254] on aluminum sheets. After development of the chromatographic plate, the detection was carried out using an Ultraviolet scanning densitometer set at a wavelength of 254 nm. The method was validated for specificity, linearity, precision, accuracy, robustness and solution stability. The method was linear in the drug the concentration range from 0.016-0.0...
The development and validation of a column high-performance liquid chromatographic assay method f... more The development and validation of a column high-performance liquid chromatographic assay method for the determination of aspirin and clopidogrel in tablet formulation are described. The combination formulation was subjected to International Conference on Harmonization-recommended stress conditions. Separation of the drugs from the degradation products formed under stress conditions was achieved on an octasilyl (C8) column using 0.3 orthophosphoric acidacetonitrile (65 + 35, v/v) mobile phase. The method was validated for specificity, linearity, limits of detection and quantification, precision, accuracy, and robustness. The method was found to be specific against placebo interference and during the forced degradation. The response was linear in the concentration range of 30.0120.0 g/mL for aspirin and 15.060.0 g/mL for clopidogrel, with a correlation coefficient of 0.9999 for both. The relative standard deviation values for intra- and interday precision were <2.0. The accuracy wa...
A novel UPLC method for determination of chlorhexidine gluconate and lidocaine hydrochloride in t... more A novel UPLC method for determination of chlorhexidine gluconate and lidocaine hydrochloride in throat sprays has been presented as a tool suitable for quality monitoring of these compounds. The developed and validated UPLC method was compared with HPLC method. Experimental parameters like analysis time, sensitivity, mobile phase consumption, validation data, and demands on instrument equipment and operation were compared for two methods. UPLC and HPLC are important methods for routine monitoring of these drugs. In UPLC, Major degradation was found in photolytic condition that product was degraded up to 30 %. The major impurity peaks was found at 3.73 min. The concentration range was varied between 0.040-0.160 mg/mL, 0.016-0.064 mg/mL for chlorhexidine gluconate and lidocaine hydrochloride respectively. The response of the drug was found to be linear in the investigation concentration range and the linear regression equation for both the technique HPLC and UPLC fitted well. The RSD ...
ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was e... more ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
International Letters of Chemistry, Physics and Astronomy, 2014
Simple and rapid reverse phase high-performance liquid chromatography (RP-HPLC) method was develo... more Simple and rapid reverse phase high-performance liquid chromatography (RP-HPLC) method was developed and validated using Phenomenex Gemini c18 (4.6 x 250 mm, 5 µ) reverse phase column for the determination of LAF in human plasma, Solid Phase Extraction (SPE) technique was used for the extraction of analyte, detection was carried out by Photo Diode Array detector at 216 nm. Chromatographic resolution of the LAF was achieved within 4.6 min by using mobile phase Methanol and 5 mM Di-Potassium Hydrogen Phosphate Buffer (pH 9.5) (80:20, v/v), flow rate was 1.0 mL/min. Calibration curve was linear with correlation coefficient of 0.9996 in the range of 50-1000 ng/mL, Limit of Detection (LOD) and Limit of Quantitation (LOQ) were 10 ng/mL and 30 ng/mL respectively, intra and inter-day deviations were lower than 3.92% and 3.98% respectively. The overall mean recovery of LAF was 94.57%. No any endogenous constituents were found to interfere at retention time of the analyte. This new RP-HPLC me...
A new generation of chroman bearing heterocyclic five membered ring such as 1,2,4-triazoles and t... more A new generation of chroman bearing heterocyclic five membered ring such as 1,2,4-triazoles and thiazolidinones was designed and synthesized. New chroman based nucleus 5-(6-fluorochroman-2-yl)-4-aryl-4H-1,2,4-triazole-3-thiol and 6-fluorochroman-N-(4-oxo-2-arylthiazolidinin-3-yl) chroman-2-carboxamides were synthesized. Aryl triazole compounds4a–4jwere synthesized from 6-fluorochroman-2-carbohydrazide2on reaction with base in methanol and CS2followed by reaction with substituted aniline. Thiazolidinone compounds5a–5jwere synthesized from 6-fluorochroman-2-carbohydrazide2on reaction with substituted aryl aldehyde and thioglycolic acid.
Journal of Liquid Chromatography & Related Technologies, 2011
... R. Patel a , Hitendra S. Joshi b &amp;amp; Atul H. Bapodra c pages 1699-1711. ... Rane , ... more ... R. Patel a , Hitendra S. Joshi b &amp;amp; Atul H. Bapodra c pages 1699-1711. ... Rane , VP ; Shinde , DB Development and Validation of Chiral LC Method for the Enantiomeric Separation of Duloxetine on Amylose Based Stationary Phase . J. Chromatogr. Sci. 2008 , 46 , 772 776 . ...
The objective of the current study was to develop a simple, precise and accurate High Perfomance ... more The objective of the current study was to develop a simple, precise and accurate High Perfomance Thin Layer Chromatographic [HPTLC] assay method and validated for determination of furosemide and spironolactone in solid pharmaceutical dosage forms. The mobile phase comprising of ethyl acetate: haxane in the volume ratio of [80: 20, v/v] was employed for the elution. Standard solution was prepared in methanol. The standard concentration was 40 µg ml -1 of furosemide and 100 µg ml -1 of spironolactone. Chromatographic analysis was performed on a HPTLC plates precoated with 0.25 mm layer of chromatographic silica gel mixture [Silica GF254] on aluminum sheets. After development of the chromatographic plate, the detection was carried out using an Ultraviolet scanning densitometer set at a wavelength of 254 nm. The method was validated for specificity, linearity, precision, accuracy, robustness and solution stability. The method was linear in the drug the concentration range from 0.016-0.0...
The development and validation of a column high-performance liquid chromatographic assay method f... more The development and validation of a column high-performance liquid chromatographic assay method for the determination of aspirin and clopidogrel in tablet formulation are described. The combination formulation was subjected to International Conference on Harmonization-recommended stress conditions. Separation of the drugs from the degradation products formed under stress conditions was achieved on an octasilyl (C8) column using 0.3 orthophosphoric acidacetonitrile (65 + 35, v/v) mobile phase. The method was validated for specificity, linearity, limits of detection and quantification, precision, accuracy, and robustness. The method was found to be specific against placebo interference and during the forced degradation. The response was linear in the concentration range of 30.0120.0 g/mL for aspirin and 15.060.0 g/mL for clopidogrel, with a correlation coefficient of 0.9999 for both. The relative standard deviation values for intra- and interday precision were <2.0. The accuracy wa...
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