O'Brien, 2021 - Google Patents
Improved Computer Vision Algorithms for High-Throughput Targeting of Single Cells in Intact Tissue for Automated MicroinjectionsO'Brien, 2021
View PDF- Document ID
- 9971307452446248804
- Author
- O'Brien J
- Publication year
External Links
Snippet
Microinjection is a technique for organism-level and cellular-level manipulation of biological systems. The precise nature of microinjection permits the ability to target single cells in intact tissue which has enabled the study of cell-type related phenomena in development and …
- 238000000520 microinjection 0 title abstract description 179
Classifications
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING; COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/30—Subject of image; Context of image processing
- G06T2207/30004—Biomedical image processing
- G06T2207/30024—Cell structures in vitro; Tissue sections in vitro
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING; COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/10—Image acquisition modality
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING; COUNTING
- G06K—RECOGNITION OF DATA; PRESENTATION OF DATA; RECORD CARRIERS; HANDLING RECORD CARRIERS
- G06K9/00—Methods or arrangements for reading or recognising printed or written characters or for recognising patterns, e.g. fingerprints
- G06K9/00127—Acquiring and recognising microscopic objects, e.g. biological cells and cellular parts
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING; COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/0002—Inspection of images, e.g. flaw detection
- G06T7/0012—Biomedical image inspection
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING; COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/20—Special algorithmic details
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN112105914B (en) | Method for automated non-invasive measurement of sperm motility and morphology and automated selection of sperm with high DNA integrity | |
Liu et al. | Robotic adherent cell injection for characterizing cell–cell communication | |
Pachitariu et al. | Suite2p: beyond 10,000 neurons with standard two-photon microscopy | |
Giurumescu et al. | Quantitative semi-automated analysis of morphogenesis with single-cell resolution in complex embryos | |
Icha et al. | Using light sheet fluorescence microscopy to image zebrafish eye development | |
Dai et al. | Automated non-invasive measurement of single sperm’s motility and morphology | |
Wu et al. | Integration of autopatching with automated pipette and cell detection in vitro | |
Wang et al. | Autofocusing and polar body detection in automated cell manipulation | |
JP6453298B2 (en) | System and method for observing and analyzing cytological specimens | |
More et al. | A semi-automated method for identifying and measuring myelinated nerve fibers in scanning electron microscope images | |
US10324080B2 (en) | Systems and methods for automated image-guided patch-clamp electrophysiology in vitro | |
Becattini et al. | A fully automated system for adherent cells microinjection | |
US11866687B2 (en) | Robotic platform for high throughput injections into intact tissue | |
Pan et al. | Automated high-productivity microinjection system for adherent cells | |
US20230101853A1 (en) | Robotic barcode tagging of distinct cell populations in intact tissue | |
Long et al. | 3D image-guided automatic pipette positioning for single cell experiments in vivo | |
Roudot et al. | u-track3D: Measuring, navigating, and validating dense particle trajectories in three dimensions | |
Duncan et al. | Isotropic light-sheet microscopy and automated cell lineage analyses to catalogue Caenorhabditis elegans embryogenesis with subcellular resolution | |
US20180128734A1 (en) | Image processing apparatus, image processing method, and image processing program | |
US20070149984A1 (en) | Injection apparatus and method | |
Jeong et al. | Measurement of ciliary beating and fluid flow in the zebrafish adult telencephalon | |
O'Brien | Improved Computer Vision Algorithms for High-Throughput Targeting of Single Cells in Intact Tissue for Automated Microinjections | |
Jaensch et al. | Automated tracking and analysis of centrosomes in early Caenorhabditis elegans embryos | |
Caldas et al. | iSBatch: a batch-processing platform for data analysis and exploration of live-cell single-molecule microscopy images and other hierarchical datasets | |
Dutta et al. | Tracking and quantifying developmental processes in C. elegans using open-source tools |