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Waldburger et al., 1994 - Google Patents

Probing the informational content of Escherichia coli σ70 region 2.3 by combinatorial cassette mutagenesis

Waldburger et al., 1994

Document ID
9605730809105005651
Author
Waldburger C
Susskind M
Publication year
Publication venue
Journal of molecular biology

External Links

Snippet

Combinatorial cassette mutagenesis was used to probe the informational content of region 2.3 of σ 70, the RNA polymerase subunit that confers promoter specificity. Region 2.3 is highly conserved among major sigmas of diverse eubacteria, and has been predicted to …
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
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