[go: up one dir, main page]

Hefley et al., 1983 - Google Patents

Enzymatic isolation of cells from neonatal calvaria using two purified enzymes from Clostridium histolyticum

Hefley et al., 1983

Document ID
9052767806826080557
Author
Hefley T
Stern P
Brand J
Publication year
Publication venue
Experimental cell research

External Links

Snippet

The enzymatic isolation of cells with bacterial collagenase has proved to be a powerful technique for the study of a wide variety of tissues. Unfortunately, for some applications such as the isolation of cells from membranous bone, the cellular damage that results from the …
Continue reading at www.sciencedirect.com (other versions)

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases Endopeptidases (3.4.21-3.4.25) derived from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)

Similar Documents

Publication Publication Date Title
Hefley et al. Enzymatic isolation of cells from neonatal calvaria using two purified enzymes from Clostridium histolyticum
Wolters et al. An analysis of the role of collagenase and protease in the enzymatic dissociation of the rat pancreas for islet isolation
O'CONNOR et al. Purification of and kinetic studies on a narrow specificity synaptosomal membrane pyroglutamate aminopeptidase from guinea‐pig brain
EP0907723B1 (en) Enzyme composition for tissue dissociation
Oza et al. Rat aortic smooth muscle cells in culture express kallikrein, kininogen, and bradykininase activity.
Esmon The subunit structure of thrombin-activated factor V. Isolation of activated factor V, separation of subunits, and reconstitution of biological activity.
Sterchi et al. N-benzoyl-L-tyrosyl-p-aminobenzoic acid hydrolase: a metalloendopeptidase of the human intestinal microvillus membrane which degrades biologically active peptides
Wolters et al. Different roles of class I and class II Clostridium histolyticum collagenase in rat pancreatic islet isolation
Kam et al. Substrate and inhibitor studies on proteinase 3
Gnosspelius Purification and properties of an extracellular protease from Myxococcus virescens
Weidman et al. Assembly of the sea urchin fertilization membrane: isolation of proteoliaisin, a calcium-dependent ovoperoxidase binding protein.
Dorbani et al. Subfractionation of the outer membrane of rat brain mitochondria: Evidence for the existence of a domain containing the porin-hexokinase complex
Erdös et al. Carboxypeptidase in blood and other fluids—III: The esterase activity of the enzyme
Kitamura Re-examination of Zymolyase purification
Kempen et al. Rat pancreas adenylate cyclase V its presence in isolated rat pancreatic acinar cells
Jany et al. Preparation of a highly purified bovine trypsin for use in protein sequence analysis
Takesuè et al. Enzymatic solubilization of microsomal NADH-cytochrome b5 reductase by lysosomes
WAYMOUTH Methods for obtaining cells in suspension from animal tissues
CA2213723A1 (en) New defined enzyme mixtures for obtaining cells and treating wounds
Bacon et al. Purification and characterisation of an aminotripeptidase from cytoplasm of Lactococcus lactis subsp. cremoris AM2
Albro et al. Purification and partial characterization of nonspecific lipase from rat pancreas
Benuck et al. A distinct peptidyl dipeptidase that degrades enkephalin: Exceptionally high activity in rabbit kidney
Emöd et al. Five sepharose‐bound ligands for the chromatographic purification of Clostridium collagenase and clostripain
Pontremoli et al. Transformation of neutral to alkaline fructose 1, 6-bisphosphatase: Converting enzyme activity in the large-particle fraction from rabbit liver
D'Aniello et al. Peptidyl-D-amino acid hydrolase from Loligo vulgaris Lam. Purification and characterization.