[go: up one dir, main page]

Sussman et al., 2004 - Google Patents

Proteomics and embryonic stem cells

Sussman et al., 2004

Document ID
874813181437793275
Author
Sussman M
Hegeman A
Harms A
Nelson C
Publication year
Publication venue
Human Embryonic Stem Cells

External Links

Snippet

This chapter focuses on such techniques using high performance liquid chromatography (HPLC) to separate complex peptide mixtures, followed by tandem mass spectrometry for sequence analysis. It discusses the vantage point of the human embryonic stem cells and …
Continue reading at www.taylorfrancis.com (other versions)

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848Methods of protein analysis involving mass spectrometry
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02Column chromatography
    • G01N30/62Detectors specially adapted therefor
    • G01N30/72Mass spectrometers
    • HELECTRICITY
    • H01BASIC ELECTRIC ELEMENTS
    • H01JELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00Particle spectrometer or separator tubes
    • H01J49/004Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn
    • H01J49/0045Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn characterised by the fragmentation or other specific reaction
    • HELECTRICITY
    • H01BASIC ELECTRIC ELEMENTS
    • H01JELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00Particle spectrometer or separator tubes
    • H01J49/02Details
    • H01J49/04Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components

Similar Documents

Publication Publication Date Title
Breuker et al. Top-down identification and characterization of biomolecules by mass spectrometry
Guerrera et al. Application of mass spectrometry in proteomics
McLachlin et al. Analysis of phosphorylated proteins and peptides by mass spectrometry
CA2278556C (en) Characterising polypeptides
EP1425586B1 (en) Mass labels
Girolamo et al. The role of mass spectrometry in the “omics” era
Lane Mass spectrometry-based proteomics in the life sciences
Reid et al. ‘Top down’protein characterization via tandem mass spectrometry
Cristoni et al. Development of new methodologies for the mass spectrometry study of bioorganic macromolecules
EP1926997B1 (en) Mass labels
Tichy et al. Phosphoproteomics: Searching for a needle in a haystack
Ashcroft Protein and peptide identification: the role of mass spectrometry in proteomics
Reid et al. Selective identification and quantitative analysis of methionine containing peptides by charge derivatization and tandem mass spectrometry
US20100311098A1 (en) Method for determining the amino acid sequence of peptides
EP1397686B1 (en) Method for characterizing polypeptides
US20030139885A1 (en) Methods and devices for proteomics data complexity reduction
Salzano et al. Mass spectrometry for protein identification and the study of post translational modifications
JP2006300758A (en) Method for quantifying target protein contained in biological sample using internal standard peptide
EP1617223A2 (en) Serial derivatization of peptides for "de Novo" sequencing using tandem mass spectrometry
Sussman et al. Proteomics and embryonic stem cells
Wei A Step-Up LC-MS/MS for Proteomics
Leijten Diving into cellular signalling: Functional proteome analysis by mass spectrometry based approaches
Shah et al. Emerging mass spectrometry-based technologies for analyses of chromatin changes: analysis of histones and histone modifications
Podtelejnikov et al. Identification of yeast proteins by mass spectrometry
Loo et al. Electrospray lonization Mass Spectrometry of Peptides and Proteins