Sussman et al., 2004 - Google Patents
Proteomics and embryonic stem cellsSussman et al., 2004
- Document ID
- 874813181437793275
- Author
- Sussman M
- Hegeman A
- Harms A
- Nelson C
- Publication year
- Publication venue
- Human Embryonic Stem Cells
External Links
Snippet
This chapter focuses on such techniques using high performance liquid chromatography (HPLC) to separate complex peptide mixtures, followed by tandem mass spectrometry for sequence analysis. It discusses the vantage point of the human embryonic stem cells and …
- 238000000575 proteomic 0 title abstract description 30
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6848—Methods of protein analysis involving mass spectrometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/72—Mass spectrometers
-
- H—ELECTRICITY
- H01—BASIC ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometer or separator tubes
- H01J49/004—Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn
- H01J49/0045—Combinations of spectrometers, tandem spectrometers, e.g. MS/MS, MSn characterised by the fragmentation or other specific reaction
-
- H—ELECTRICITY
- H01—BASIC ELECTRIC ELEMENTS
- H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
- H01J49/00—Particle spectrometer or separator tubes
- H01J49/02—Details
- H01J49/04—Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Breuker et al. | Top-down identification and characterization of biomolecules by mass spectrometry | |
Guerrera et al. | Application of mass spectrometry in proteomics | |
McLachlin et al. | Analysis of phosphorylated proteins and peptides by mass spectrometry | |
CA2278556C (en) | Characterising polypeptides | |
EP1425586B1 (en) | Mass labels | |
Girolamo et al. | The role of mass spectrometry in the “omics” era | |
Lane | Mass spectrometry-based proteomics in the life sciences | |
Reid et al. | ‘Top down’protein characterization via tandem mass spectrometry | |
Cristoni et al. | Development of new methodologies for the mass spectrometry study of bioorganic macromolecules | |
EP1926997B1 (en) | Mass labels | |
Tichy et al. | Phosphoproteomics: Searching for a needle in a haystack | |
Ashcroft | Protein and peptide identification: the role of mass spectrometry in proteomics | |
Reid et al. | Selective identification and quantitative analysis of methionine containing peptides by charge derivatization and tandem mass spectrometry | |
US20100311098A1 (en) | Method for determining the amino acid sequence of peptides | |
EP1397686B1 (en) | Method for characterizing polypeptides | |
US20030139885A1 (en) | Methods and devices for proteomics data complexity reduction | |
Salzano et al. | Mass spectrometry for protein identification and the study of post translational modifications | |
JP2006300758A (en) | Method for quantifying target protein contained in biological sample using internal standard peptide | |
EP1617223A2 (en) | Serial derivatization of peptides for "de Novo" sequencing using tandem mass spectrometry | |
Sussman et al. | Proteomics and embryonic stem cells | |
Wei | A Step-Up LC-MS/MS for Proteomics | |
Leijten | Diving into cellular signalling: Functional proteome analysis by mass spectrometry based approaches | |
Shah et al. | Emerging mass spectrometry-based technologies for analyses of chromatin changes: analysis of histones and histone modifications | |
Podtelejnikov et al. | Identification of yeast proteins by mass spectrometry | |
Loo et al. | Electrospray lonization Mass Spectrometry of Peptides and Proteins |