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Lejeune et al., 1986 - Google Patents

Cloning of an endoglucanase gene from Pseudomonas fluorescens var. cellulosa into Escherichia coli and Pseudomonas fluorescens

Lejeune et al., 1986

Document ID
8598350338481657535
Author
Lejeune A
Colson C
Eveleigh D
Publication year
Publication venue
Journal of industrial microbiology and biotechnology

External Links

Snippet

An endoglucanase chromosomal gene from the cellulolytic Pseudomonas fluorescens var. cellulosa (NCIB 10462) was cloned in Escherichia coli. Chromosomal DNA was partially digested with the restriction enzyme Eco RI and ligated into the broad host-range …
Continue reading at academic.oup.com (other versions)

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    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
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    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
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