[go: up one dir, main page]

Nelson, 2018 - Google Patents

Analysis of Fragmented and Unfragmented Human and Porcine Immunoglobulin G (IgG) N-Glycoforms by Matrix-Assisted Laser Desorption Ionization Time-of-Flight …

Nelson, 2018

View PDF
Document ID
7728943927037572942
Author
Nelson C
Publication year

External Links

Snippet

A developed workflow for detailed structural characterization of antibodies from different sources, using methods which involve MS is discussed in this thesis. The aim of this thesis is to emphasize the usefulness of detailed characterization of glycoforms of human and pig …
Continue reading at mspace.lib.umanitoba.ca (PDF) (other versions)

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
    • G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay
    • G01N33/558—Immunoassay; Biospecific binding assay using diffusion or migration of antigen or antibody
    • G01N33/561—Immunoelectrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • G01N30/461—Flow patterns using more than one column with serial coupling of separation columns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories

Similar Documents

Publication Publication Date Title
Srebalus Barnes et al. Applications of mass spectrometry for the structural characterization of recombinant protein pharmaceuticals
AU2012315956B2 (en) Intact mass determination of protein conjugated agent compounds
US11598756B2 (en) Platform for native liquid chromatography-mass spectrometry
US11932684B2 (en) Online chromatography and electrospray ionization mass spectrometer
WO2014121031A1 (en) Characterization of antibody mixtures by mass spectrometry
Pabst et al. A microarray-matrix-assisted laser desorption/ionization-mass spectrometry approach for site-specific protein N-glycosylation analysis, as demonstrated for human serum immunoglobulin M (IgM)
EP2909632A1 (en) Improved method of mapping glycans of glycoproteins
AU2021205910A1 (en) Use of amino acids to enhance signal in mass spectral analyses
Nelson Analysis of Fragmented and Unfragmented Human and Porcine Immunoglobulin G (IgG) N-Glycoforms by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry and Ultra Performance Liquid Chromatography Mass Spectrometry
EP4062176B1 (en) Label-free n-glycan quantification methods
JP2022549875A (en) Hydrophobic Interaction Chromatography-Binding Native Mass Spectrometry for Antibody Analysis
US20230018713A1 (en) Characterization of proteins by anion-exchange chromatography mass spectrometry (aex-ms)
Richter et al. Mass spectrometric analysis of protein species of biologics
Birx Analysis of N-and O-Linked Glycopeptides
CN118076890A (en) Protein Characterization by Anion Exchange Chromatography-Mass Spectrometry (AEX-MS)
EA047025B1 (en) USING AMINO ACIDS TO Amplify SIGNALS IN MASS SPECTRAL ANALYSIS
EA045776B1 (en) PLATFORM FOR NATIVE LIQUID CHROMATO-MASS SPECTROMETRY
EA046855B1 (en) ONLINE CHROMATOGRAPHY AND ELECTROSPRAY IONIZATION MASS SPECTROMETER
Selman Glycosylation analysis of immunoglobulin G: method development and clinical applications
Xiang Analysis of Complex Oligosaccharides from Glycopeptides and Glycoproteins Using MSn Spectra and Oligosaccharides Spectral Library
HK1256475B (en) Intact mass determination of protein conjugated agent compounds