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Snustad et al., 1988 - Google Patents

Maize glutamine synthetase cDNAs: isolation by direct genetic selection in Escherichia coli.

Snustad et al., 1988

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Document ID
6500283592292744874
Author
Snustad D
Hunsperger J
Chereskin B
Messing J
Publication year
Publication venue
Genetics

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Maize glutamine synthetase cDNA clones were isolated by genetic selection for functional rescue of an Escherichia coli delta glnA mutant growing on medium lacking glutamine. The Black Mexican Sweet cDNA library used in this study was constructed in pUC13 such that …
Continue reading at pmc.ncbi.nlm.nih.gov (PDF) (other versions)

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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