Zeng et al., 1997 - Google Patents
Improved Stability and Electrophoretic Properties of Preformed Fluorescent Cationic Dye–DNA Complexes in a Taps–Tetrapentylammonium Buffer in Agarose Slab …Zeng et al., 1997
- Document ID
- 6067536011826842431
- Author
- Zeng Z
- Clark S
- Mathies R
- Glazer A
- Publication year
- Publication venue
- Analytical biochemistry
External Links
Snippet
High-resolution capillary electrophoresis sizing of preformed complexes of bis-intercalating fluorescent dyes with double-stranded DNA has been demonstrated using hydroxyethylcellulose and 3-[tris (hydroxymethyl) methylamino]-1-propanesulfonic acid …
- 229920003013 deoxyribonucleic acid 0 title abstract description 43
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
- G01N27/44721—Arrangements for investigating the separated zones, e.g. localising zones by optical means
- G01N27/44726—Arrangements for investigating the separated zones, e.g. localising zones by optical means using specific dyes, markers or binding molecules
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44743—Introducing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44708—Cooling
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay
- G01N33/558—Immunoassay; Biospecific binding assay using diffusion or migration of antigen or antibody
- G01N33/561—Immunoelectrophoresis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for mutation or polymorphism detection
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US5312921A (en) | Dyes designed for high sensitivity detection of double-stranded DNA | |
| US5783687A (en) | Dyes designed for high sensitivity detection of double-stranded DNA | |
| Schwartz et al. | Capillary electrophoresis with laser-induced fluorescence detection of PCR fragments using thiazole orange | |
| Wang et al. | Rapid sizing of short tandem repeat alleles using capillary array electrophoresis and energy-transfer fluorescent primers | |
| US5370777A (en) | Capillary column containing removable separation gel composition and method of use | |
| McCord et al. | Capillary electrophoresis of polymerase chain reaction-amplified DNA using fluorescence detection with an intercalating dye | |
| Milofsky et al. | Native fluorescence detection of nucleic acids and DNA restriction fragments in capillary electrophoresis | |
| US5767267A (en) | DNA complexes with dyes designed for energy transfer as fluorescent markers | |
| US5869255A (en) | Probes labeled with energy transfer couples dyes exemplified with DNA fragment analysis | |
| Kim et al. | Separation of nucleic acids by capillary electrophoresis in cellulose solutions with mono-and bisintercalating dyes | |
| Clark et al. | Multiplex dsDNA Fragment Sizing Using Dimeric Intercalation Dyes and Capillary Array Electrophoresis: Ionic Effects on the Stability and Electrophoretic Mobility of DNA− Dye Complexes | |
| US6887668B2 (en) | Nucleic acid separation and detection by electrophoresis with a counter-migrating high-affinity intercalating dye | |
| Zeng et al. | Improved Stability and Electrophoretic Properties of Preformed Fluorescent Cationic Dye–DNA Complexes in a Taps–Tetrapentylammonium Buffer in Agarose Slab Gels | |
| EP0464544B1 (en) | Neutral and positively charged dyes for electrophoresis sample loading solutions | |
| Zeng et al. | Fluorescence energy-transfer cyanine heterodimers with high affinity for double-stranded DNA II. Applications to multiplex restriction fragment sizing | |
| Jin et al. | Miniaturized electrophoresis: an evolving role in laboratory medicine | |
| Boublǐková et al. | Determination of submicrogram quantities of circular duplex DNA in plasmid samples by adsorptive stripping voltammetry | |
| Rodríguez-Plaza et al. | Combining microsatellite markers and capillary gel electrophoresis with laser-induced fluorescence to identify the grape (Vitis vinifera) variety of musts | |
| EP0497448A1 (en) | Capillary gel electrophoresis in the presence of at least one neutral non-urea denaturing agent | |
| Zhang et al. | Fast separation of single‐stranded oligonucleotides by capillary electrophoresis using OliGreen® as fluorescence inducing agent | |
| WO1993015394A1 (en) | Capillary column containing removable separation gel composition and method of use | |
| Shin et al. | Separation and size determination of circular and linear single-stranded DNAs by alkaline agarose gel electrophoresis | |
| Ren | High throughput screening of genetic mutations/polymorphisms by capillary electrophoresis | |
| Ren et al. | Inverse-flow derivatization for capillary electrophoresis of DNA fragments with laser-induced fluorescence detection | |
| US6228655B1 (en) | Method for detection of DNA on polymer gels using a counter-dye composition and counter-dye composition for the same |