Woo et al., 2022 - Google Patents
High-throughput high-dynamic range imaging by spatiotemporally structured illuminationWoo et al., 2022
View HTML- Document ID
- 5498109335606417217
- Author
- Woo T
- Kim H
- Kim S
- Hwang B
- Ahn C
- Kwon S
- Kim J
- Park J
- Publication year
- Publication venue
- APL Photonics
External Links
Snippet
Recent advances in biochemistry and optics have enabled observation of the faintest signals from even single molecules. However, although biological samples can have varying degrees of fluorescence expression ranging from a single to thousands of …
- 238000003384 imaging method 0 title abstract description 15
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
- G02B21/367—Control or image processing arrangements for digital or video microscopes providing an output produced by processing a plurality of individual source images, e.g. image tiling, montage, composite images, depth sectioning, image comparison
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultra-violet illumination; Fluorescence microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/10—Condensers affording dark-field illumination
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING; COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/30—Subject of image; Context of image processing
- G06T2207/30004—Biomedical image processing
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING; COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/10—Image acquisition modality
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
-
- G—PHYSICS
- G06—COMPUTING; CALCULATING; COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Mandracchia et al. | Fast and accurate sCMOS noise correction for fluorescence microscopy | |
Wu et al. | Three-dimensional virtual refocusing of fluorescence microscopy images using deep learning | |
Mahecic et al. | Homogeneous multifocal excitation for high-throughput super-resolution imaging | |
US10352860B2 (en) | Super resolution microscopy | |
Ingaramo et al. | Two-photon excitation improves multifocal structured illumination microscopy in thick scattering tissue | |
US9435993B2 (en) | Three dimensional microscopy imaging | |
Zhao et al. | Enhanced detection of fluorescence fluctuations for high-throughput super-resolution imaging | |
US12216055B2 (en) | Microscope image capturing methods | |
JP7649648B2 (en) | Method for imaging a sample using a fluorescence microscope with stimulated emission depletion - Patents.com | |
US8217992B2 (en) | Microscopic imaging techniques | |
Chang et al. | Real-time multi-angle projection imaging of biological dynamics | |
US20160086027A1 (en) | Method and apparatus for single-particle localization using wavelet analysis | |
Zhang et al. | Rapid detection of neurons in widefield calcium imaging datasets after training with synthetic data | |
JP5259207B2 (en) | Cell image analysis apparatus and method and software thereof | |
US10416427B2 (en) | Scan-based imaging with variable scan speed using predictions of region-of-interest positions | |
JP7277051B2 (en) | Transillumination imaging with the use of interference fringes to enhance contrast and find focus | |
JP2020535478A (en) | Double-pass macro image | |
Nöbauer et al. | Mesoscale volumetric light-field (MesoLF) imaging of neuroactivity across cortical areas at 18 Hz | |
Lu et al. | A practical guide to scanning light-field microscopy with digital adaptive optics | |
JP6928757B2 (en) | Systems and methods for image processing in light microscopy | |
Yoon et al. | Simultaneous multicolor multifocal scanning microscopy | |
US10776955B2 (en) | Method for the analysis of spatial and temporal information of samples by means of optical microscopy | |
Delattre | Igniting new confocal imaging potential–Nikon AX R series with NSPARC | |
Woo et al. | High-throughput high-dynamic range imaging by spatiotemporally structured illumination | |
Cha et al. | Reassignment of scattered emission photons in multifocal multiphoton microscopy |