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Bowers et al., 2004 - Google Patents

Bacterial expression system with tightly regulated gene expression and plasmid copy number

Bowers et al., 2004

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Document ID
5362034023517785354
Author
Bowers L
LaPoint K
Anthony L
Pluciennik A
Filutowicz M
Publication year
Publication venue
Gene

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Snippet

A new Escherichia coli host/vector system has been engineered to allow tight and uniform modulation of gene expression and γ origin (ori) plasmid copy number. Regulation of γ ori plasmid copy number is achieved through arabinose-inducible expression of the necessary …
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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