[go: up one dir, main page]

Girschick et al., 2002 - Google Patents

The kappa gene repertoire of human neonatal B cells

Girschick et al., 2002

Document ID
4784266244935606780
Author
Girschick H
Lipsky P
Publication year
Publication venue
Molecular Immunology

External Links

Snippet

The kappa chain repertoire of individual IgD+ human neonatal B cells was analyzed using a single cell PCR technique. A total of 104 productive and 90 non-productive VκJκ rearrangements from three cord blood B cell samples were sequenced and compared to the …
Continue reading at www.sciencedirect.com (other versions)

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material Not used, see subgroups
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells

Similar Documents

Publication Publication Date Title
Martin et al. Positive selection from newly formed to marginal zone B cells depends on the rate of clonal production, CD19, and btk
Bakkus et al. Evidence that the clonogenic cell in multiple myeloma originates from a pre‐switched but somatically mutated B cell
Yang et al. Distinct mechanisms define murine B cell lineage immunoglobulin heavy chain (IgH) repertoires
Bräuninger et al. Regulation of immunoglobulin light chain gene rearrangements during early B cell development in the human
Brezinschek et al. Analysis of the human VH gene repertoire. Differential effects of selection and somatic hypermutation on human peripheral CD5 (+)/IgM+ and CD5 (-)/IgM+ B cells.
Berkowska et al. Human memory B cells originate from three distinct germinal center-dependent and-independent maturation pathways
Hardy et al. Distinctive developmental origins and specificities of murine CD5+ B cells
Pascual et al. VH restriction among human cold agglutinins. The VH4-21 gene segment is required to encode anti-I and anti-i specificities
Mantovani et al. Human rheumatoid B-1a (CD5+ B) cells make somatically hypermutated high affinity IgM rheumatoid factors.
Ghraichy et al. Maturation of the human immunoglobulin heavy chain repertoire with age
Dammers et al. Most marginal zone B cells in rat express germline encoded Ig VH genes and are ligand selected
Feeney Junctional sequences of fetal T cell receptor beta chains have few N regions.
Mortari et al. The human cord blood antibody repertoire. Frequent usage of the VH7 gene family
Ghraichy et al. B‐cell receptor repertoire sequencing in patients with primary immunodeficiency: a review
US20070160994A1 (en) Repertoire determination of a lymphocyte b population
Fischer et al. Molecular single-cell analysis reveals that CD5-positive peripheral blood B cells in healthy humans are characterized by rearranged Vkappa genes lacking somatic mutation.
Girschick et al. The kappa gene repertoire of human neonatal B cells
Tian et al. Evidence for preferential Ig gene usage and differential TdT and exonuclease activities in human naive and memory B cells
Levy et al. Functional immunoglobulin light chain genes are replaced by ongoing rearrangements of germline V kappa genes to downstream J kappa segment in a murine B cell line.
Tobin et al. VH3-21 Gene Usage in Chronic Lymphocytic Leukemia–Characterization of a New Subgroup with Distinct Molecular Features and Poor Survival
Hardy et al. Developmental origins, specificities and immunoglobulin gene biases of murine Ly-1 B cells
Feeney et al. Human cord blood kappa repertoire.
Collins et al. Immunoglobulin gene rearrangement, repertoire diversity, and the allergic response
Andris et al. Variable region gene segment utilization in rhesus monkey hybridomas producing human red blood cell-specific antibodies: predominance of the VH4 family but not VH4-21 (V4-34)
Schutte et al. VH4. 21-encoded natural autoantibodies with anti-i specificity mirror those associated with cold hemagglutinin disease.