Satomura et al., 1988 - Google Patents
α-Amylase assay with use of a benzyl derivative of p-nitrophenyl α-maltopentaoside, BG5PSatomura et al., 1988
- Document ID
- 4455817041931546819
- Author
- Satomura S
- Sakata Y
- Omichi K
- Ikenaka T
- Publication year
- Publication venue
- Clinica chimica acta
External Links
Snippet
Abstract P-Nitrophenyl O-(6-O-benzyl)-α-D-glucopyranosyl-(1→ 4)-O-α-D-glucopyranosyl- (1→ 4)-O-α-D-glucopyranosyl-(1→(BG5P) is hydrolyzed by both human salivary α-amylase (HSA) and human pancreatic a-amylase (HPA) to O-(6-O-benzyl)-α-D-glucopyranosyl-(1→ …
- 238000004166 bioassay 0 title abstract description 25
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
- G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/978—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/60—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving cholesterol
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Eivazi et al. | Glucosidases and galactosidases in soils | |
Winn-Deen et al. | Development of a direct assay for alpha-amylase. | |
Gella et al. | Determination of total and pancreatic α-amylase in human serum with 2-chloro-4-nitrophenyl-α-D-maltotrioside as substrate | |
Allain et al. | Enzymatic determination of total serum cholesterol | |
Hägele et al. | Mechanism of action of human pancreatic and salivary alpha-amylase on alpha-4-nitrophenyl maltoheptaoside substrate. | |
JPS6163299A (en) | Detection of alpha amylase | |
Dupuy et al. | Rapid determination of alpha-amylase activity by use of a new chromogenic substrate. | |
IE47953B1 (en) | Process and reagent for the determination of -amylase | |
US4000042A (en) | Diagnostic reagent for the determination of amylase | |
Satomura et al. | α-Amylase assay with use of a benzyl derivative of p-nitrophenyl α-maltopentaoside, BG5P | |
Junge et al. | Development and evaluation of assays for the determination of total and pancreatic amylase at 37 C according to the principle recommended by the IFCC | |
CA1332806C (en) | Chromogenic substrates | |
Kaoru et al. | Differential assay of human pancreatic and salivary α-amylases in serum using a new fluorogenic substrate | |
KR900005623B1 (en) | Method for measuring NAG azease and reagents thereof | |
US5202233A (en) | Process for the detection of substances with hydrolase activity | |
JPS637196B2 (en) | ||
US5350678A (en) | Method of differential assay for α-amylase isozymes and a kit for the same | |
Wallenfels et al. | Action pattern of human pancreatic and salivary α-amylase on 1, 4-α-D-nitrophenylmaltooligosaccharides. 1, 4-α-D-nitrophenylmaltooligosaccharides as substrates of α-amylase, I | |
EP0262707B1 (en) | Test reagent for amylase determination | |
Cook et al. | Benzyl p-guanidinothiobenzoate hydrochloride, a new active-site titrant for trypsin and trypsin-like enzymes | |
JPS63214193A (en) | Production of 6-glucosylmaltooligosaccharide derivative and measurement of alpha-amylase activity using said derivative | |
US5260428A (en) | Agent for the detection of substances with hydrolase activity | |
Gubern et al. | Production and certification of an enzyme reference material for pancreatic α-amylase (CRM 476) | |
Ben-Yoseph et al. | Lysosomal enzyme activities in fresh and frozen chorionic villi and in cultured trophoblasts | |
EP0359138B1 (en) | Stabilization and reduction of background fluorescence of hydroxy coumarin ester enzyme substrates |