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Elsea et al., 1995 - Google Patents

A yeast type II topoisomerase selected for resistance to quinolones: mutation of histidine 1012 to tyrosine confers resistance to nonintercalative drugs but …

Elsea et al., 1995

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Document ID
4275465672217552379
Author
Elsea S
Hsiung Y
Nitiss J
Osheroff N
Publication year
Publication venue
Journal of Biological Chemistry

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A mutant yeast type II topoisomerase was generated by in vitro mutagenesis followed by selection in vivo for resistance to the quinolone CP-115,953. The resulting mutant enzyme had a single point mutation which converted His 1012 to Tyr (top2H1012Y). top2H1012Y …
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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