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Sgaramella et al., 1978 - Google Patents

Use of the T4 polynucleotide ligase in the joining of flush‐ended DNA segments generated by restriction endonucleases

Sgaramella et al., 1978

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Document ID
4062440636393030081
Author
Sgaramella V
Ehrlich S
Publication year
Publication venue
European Journal of Biochemistry

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Snippet

Double‐stranded DNA segments with completely base‐paired ends were obtained by the action of various restriction endonucleases on phage and plasmid DNAs. These segments were joined covalently by the T4 polynucleotide ligase. The joining was monitored by the …
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    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
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