Hsu, 1983 - Google Patents
Purification and partial characterization of ATP pyrophosphohydrolase from fetal bovine epiphyseal cartilage.Hsu, 1983
View PDF- Document ID
- 3960087585129067550
- Author
- Hsu H
- Publication year
- Publication venue
- Journal of Biological Chemistry
External Links
Snippet
ATP pyrophosphohydrolase was partially purified from fetal bovine epiphyseal cartilage. The purification was about 10-and 100-fold over the enzyme activities of matrix vesicle fraction and cell homogenate, respectively. The pyrophosphohydrolase and alkaline phosphatase …
- 108010006548 ATP pyrophosphohydrolase 0 title abstract description 35
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/42—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving transferase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/814—Enzyme separation or purification
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Hsu | Purification and partial characterization of ATP pyrophosphohydrolase from fetal bovine epiphyseal cartilage. | |
Bustamante et al. | Energy metabolism of tumor cells. Requirement for a form of hexokinase with a propensity for mitochondrial binding. | |
Johnson et al. | Cellular localization and characterization of bovine liver branched-chain α-keto acid dehydrogenases | |
DiPietro et al. | Separation and properties of three acid phosphatases from human placenta | |
Mayes et al. | Differential assay for lysosomal alpha-galactosidases in human tissues and its application to Fabry's disease | |
Horvat et al. | On the lysosomal occurrence and the properties of the neuraminidase of rat liver and of Ehrlich ascites tumor cells | |
Hayman et al. | Purification and characterization of a tartrate-resistant acid phosphatase from human osteoclastomas | |
Albert et al. | Biosynthesis of 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine (platelet-activating factor) from 1-alkyl-2-acyl-sn-glycero-3-phosphocholine by rat alveolar macrophages. Phospholipase A2 and acetyltransferase activities during phagocytosis and ionophore stimulation. | |
DeMartino et al. | Identification and partial purification of an ATP-stimulated alkaline protease in rat liver. | |
Wiederschain et al. | Characterization of 6-hexadecanoylamino-4-methylumbelliferyl-β-D-galactopyranoside as fluorogenic substrate of galactocerebrosidase for the diagnosis of Krabbe disease | |
Anchors et al. | Purification of cerebral glucose-6-phosphatase. An enzyme involved in sleep | |
Brightwell et al. | Subcellular distributions and properties of rat liver phosphodiesterases | |
Levade et al. | Sphingomyelinases and Niemann-Pick disease | |
Wolpert et al. | The role of phosphohydrolases in the mechanism of resistance of neoplastic cells to 6-thiopurines | |
Baldwin et al. | Rat kidney glycerylphosphorylcholine diesterase | |
Hashitani et al. | The partial purification of thiamine triphosphatase from rat brain | |
Segal et al. | Liver galactose-l-phosphate uridyl transferase: activity in normal and galactosemic subjects | |
Samuels et al. | Hydrolytic cleavage of methotrexate γ-polyglutamates by folylpolyglutamyl hydrolase derived from various tumors and normal tissues of the mouse | |
Mueller et al. | beta-Glucoside hydrolase activity of normal and glucosylceramidotic cultured human skin fibroblasts. | |
Smith et al. | Phosphorylation of rat liver nuclear envelopes. I. Characterization of in vitro protein phosphorylation. | |
Bach et al. | Pseudodeficiency of α‐galactosidase A | |
Fabre et al. | Hydroxylation of 4-amino-antifolates by partially purified aldehyde oxidase from rabbit liver | |
De Jong | Partial purification and properties of rat-heart adenosine kinase | |
Lee et al. | Triphosphoinositide phosphomonoesterase in rat kidney cortex: I. General properties and subcellular localization | |
Nosaka et al. | Cisplatin-induced alterations in renal structure, ammoniagenesis and gluconeogenesis of rats |