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Black, 1981 - Google Patents

In vitro packaging of bacteriophage T4 DNA

Black, 1981

Document ID
3705659831445523601
Author
Black L
Publication year
Publication venue
Virology

External Links

Snippet

Bacteriophage T4 can be formed in an in vitro DNA packaging system which requires addition of exogenous T4 DNA. Extracts prepared from bacteria infected with mutants 20cs, 17cs, 16am, 17am, or 16am–17am contain processed proheads. These appear to be …
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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