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Cheng et al., 2016 - Google Patents

Enhancement of a high efficient autoinducible expression system in Bacillus subtilis by promoter engineering

Cheng et al., 2016

Document ID
3317898343521113675
Author
Cheng J
Guan C
Cui W
Zhou L
Liu Z
Li W
Zhou Z
Publication year
Publication venue
Protein Expression and Purification

External Links

Snippet

Quorum-sensing related promoter srfA (P srfA) was used to construct autoinducible expression system for production of recombinant proteins in Bacillus subtilis. P srfA was prominent in the unique property of inducer-free activity that is closely correlated with cell …
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
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    • C12N15/70—Vectors or expression systems specially adapted for E. coli
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6897—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
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