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Tjong et al., 2013 - Google Patents

Direct fluorescence detection of RNA on microarrays by surface-initiated enzymatic polymerization

Tjong et al., 2013

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Document ID
2176202436658831105
Author
Tjong V
Yu H
Hucknall A
Chilkoti A
Publication year
Publication venue
Analytical Chemistry

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Snippet

We report the first demonstration of surface-initiated enzymatic polymerization (SIEP) for the direct detection of RNA in a fluorescence microarray format. This new method incorporates multiple fluorophores into an RNA strand using the two-step sequential and complementary …
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
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    • C12Q1/6834—Nucleic acid analysis involving immobilisation; Immobilisation characterised by the carrier or coupling agent
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