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Dioubankova et al., 2004 - Google Patents

Detection of point mutations using pyrene-labeled DNA probes

Dioubankova et al., 2004

Document ID
2168025906227707043
Author
Dioubankova N
Malakhov A
Stetsenko D
Korshun V
Publication year
Publication venue
Russian chemical bulletin

External Links

Snippet

Pyrene-labeled oligodeoxyribonucleotide probes were shown to be suitable for the detection of point mutations. Reagents based on homochiral 2, 4-dihydroxybutyramides were used to introduce pyrene residues at the 3"-and 5"-ends of oligonucleotide pairs. The …
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the means of detection
    • C12Q1/6818—Hybridisation assays characterised by the means of detection involving interaction of at least two labels, e.g. resonant energy transfer
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