Guan et al., 1992 - Google Patents
Studies on the isolation of penicillin acylase from Escherichia coli by aqueous two‐phase partitioningGuan et al., 1992
- Document ID
- 1932974163889644122
- Author
- Guan Y
- Wu X
- Treffry T
- Lilley T
- Publication year
- Publication venue
- Biotechnology and bioengineering
External Links
Snippet
A method of enzyme release and aqueous two‐phase extraction is described for the separation of penicillin acylase from Escherichia coli cells. Butyl acetate, 12%(v/v), treatment combined with freeze–thawing gives up to 70% enzyme release. For polyethylene glycol …
- 108010073038 EC 3.5.1.11 0 title abstract description 39
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/30—Extraction; Separation; Purification by precipitation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
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