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Bender et al., 1992 - Google Patents

IS10 transposase mutations that specifically alter target site recognition.

Bender et al., 1992

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Document ID
1807931546980647587
Author
Bender J
Kleckner N
Publication year
Publication venue
The EMBO journal

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Snippet

IS10 inserts preferentially into particular hotspots. We describe here mutations of IS10 transposase, called 'ATS'that confer Altered Target Specificity. These mutations yield a general relaxation in target specificity but do not affect other aspects of transposition. Thus …
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
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