Leibler et al., 1996 - Google Patents
Salicylaldehyde–metal–amino acid ternary complex: A new tool for immobilized metal affinity chromatographyLeibler et al., 1996
- Document ID
- 17573378370013135364
- Author
- Leibler D
- Rabinkov A
- Wilchek M
- Publication year
- Publication venue
- Journal of Molecular Recognition
External Links
Snippet
An immobilized salicylaldehyde (sal) was used to build various salicylaldehyde–copper– amino acid (Sal–Cu–AA) complexes which are stable at a range of pH values (2.0–11.0). The complexes were found to bind protein molecules as IMAC resins. Thirteen proteins were …
- 238000001597 immobilized metal affinity chromatography 0 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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