[go: up one dir, main page]

Guo et al., 2014 - Google Patents

Development of chromatofocusing techniques employing mixed-mode column packings for protein separations

Guo et al., 2014

View PDF
Document ID
17560462693097815676
Author
Guo H
Li X
Frey D
Publication year
Publication venue
Journal of Chromatography A

External Links

Snippet

Recent studies reported in the literature using mixed-mode chromatography (MMC) column packings have shown that multiple modes of interactions between the column packing and proteins can be usefully exploited to yield excellent resolution as well as salt-tolerant …
Continue reading at hpcf-files.umbc.edu (PDF) (other versions)

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/28—Control of physical parameters of the fluid carrier
    • G01N30/34—Control of physical parameters of the fluid carrier of fluid composition, e.g. gradient
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/26—Conditioning of the fluid carrier; Flow patterns
    • G01N30/38—Flow patterns
    • G01N30/46—Flow patterns using more than one column
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3847—Multimodal interactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/18—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to flow patterns
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis

Similar Documents

Publication Publication Date Title
Eriksson Hydrophobic interaction chromatography
Maria et al. Purification process of recombinant monoclonal antibodies with mixed mode chromatography
Fekete et al. Method development for the separation of monoclonal antibody charge variants in cation exchange chromatography, Part II: pH gradient approach
Ghose et al. Evaluation and comparison of alternatives to Protein A chromatography: Mimetic and hydrophobic charge induction chromatographic stationary phases
Talebi et al. Charge heterogeneity profiling of monoclonal antibodies using low ionic strength ion-exchange chromatography and well-controlled pH gradients on monolithic columns
Krättli et al. Online control of the twin-column countercurrent solvent gradient process for biochromatography
Shan et al. Gradient chromatofocusing. Versatile pH gradient separation of proteins in ion-exchange HPLC: Characterization studies
Holstein et al. Improving selectivity in multimodal chromatography using controlled pH gradient elution
Guo et al. Development of chromatofocusing techniques employing mixed-mode column packings for protein separations
Müller-Späth et al. Model simulation and experimental verification of a cation-exchange IgG capture step in batch and continuous chromatography
Fekete et al. Characterization of cation exchanger stationary phases applied for the separations of therapeutic monoclonal antibodies
Karlsson et al. 4 Ion Exchange Chromatography
Ahamed et al. Selection of pH-related parameters in ion-exchange chromatography using pH-gradient operations
Gajdosik et al. Sample displacement chromatography as a method for purification of proteins and peptides from complex mixtures
Shan et al. Effect of buffer concentration on gradient chromatofocusing performance separating proteins on a high-performance DEAE column
McCue et al. Modeling of protein monomer/aggregate purification and separation using hydrophobic interaction chromatography
Voitl et al. Application of mixed mode resins for the purification of antibodies
Brochier et al. Fast purification process optimization using mixed-mode chromatography sorbents in pre-packed mini-columns
Muthukumar et al. High throughput process development (HTPD) platform for membrane chromatography
Lee et al. Modeling of dual gradient elution in ion exchange and mixed-mode chromatography
CA3005484A1 (en) Opposite ph-salt gradients for improved protein separations
Grönberg Ion exchange chromatography
Kumar et al. Two-stage chromatographic separation of aggregates for monoclonal antibody therapeutics
Helling et al. Optimal integration of directly combined hydrophobic interaction and ion exchange chromatography purification processes
Marek et al. Isolation of monoclonal antibody from a Chinese hamster ovary supernatant. I: Assessment of different separation concepts