Ohshita et al., 1993 - Google Patents
Analysis of lysosomal degradation of fluorescein isothiocyanate-labelled proteins by Toyopearl HW-40 affinity chromatographyOhshita et al., 1993
- Document ID
- 16288747690288976223
- Author
- Ohshita T
- Katunuma N
- Publication year
- Publication venue
- Journal of Chromatography A
External Links
Snippet
Fluorescein isothiocyanate (FITC) was found to have a strong affinity to Toyopearl gel, which is used for gel filtration. FITC-labelled amino acids also showed affinity to Toyopearl gel, their elution from a Toyopearl HW-40 column being retarded. On the other hand, FITC …
- 102000004169 proteins and genes 0 title abstract description 15
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Hybridisation probes
- C12Q1/6888—Hybridisation probes for detection or identification of organisms
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Hurley et al. | Purification and characterization of the gamma regulatory subunit of the cyclic GMP phosphodiesterase from retinal rod outer segments. | |
Spanos et al. | Detection of the catalytic activities of DNA polymerases and their associated exonucleases following SDS-polyacrylamide gel electrophoresis | |
Nielsen et al. | Purification of zymogen to plasminogen activator from human glioblastoma cells by affinity chromatography with monoclonal antibody | |
Rinderknecht et al. | Mesotrypsin: A New Inhibitor-Resistant Protease From, a Zymogen in Human Pancreatic Tissue and Fluid | |
Cho et al. | Two soluble forms of glutamate dehydrogenase isoproteins from bovine brain | |
Blocher et al. | Multiple modes of inhibition of spore germination and outgrowth by reduced pH and sorbate | |
Camargo et al. | Brain peptidases: conversion and inactivation of kinin hormones | |
Kincaid et al. | Proteolytic activation of calmodulin-dependent cyclic nucleotide phosphodiesterase. | |
Takahashi-Nakamura et al. | Purification and characterization of an inhibitor of calcium-activated neutral protease from rabbit skeletal muscle | |
Merlin et al. | The interferon-induced enzyme oligo-isoadenylate synthetase: rapid determination of its in vitro products | |
Fujimura et al. | Purification and properties of a bacteriocin-like substance (acnecin) of oral Propionibacterium acnes | |
Nomura et al. | Purification and primary structure of a new guanylic acid specific ribonuclease from Pleurotus ostreatus | |
Mishima et al. | Arginine-specific ADP-ribosyltransferase and its acceptor protein p33 in chicken polymorphonuclear cells: co-localization in the cell granules, partial characterization, and in situ mono (ADP-ribosyl) ation | |
Voytek et al. | Studies of an anionic trypsinogen and its active enzyme from porcine pancreas | |
Kohno et al. | Immunoaffinity purification and characterization of leucine aminopeptidase from human liver. | |
Porumb et al. | Structural and catalytic properties of L-alanine dehydrogenase from Bacillus cereus. | |
McLay | Activities of cathepsins A and D in cod muscle | |
Dizik et al. | Avocado polyphenoloxidase: Purification, and fractionation on Sephadex thin layers | |
CRAFTS‐LIGHTY et al. | The structure and function of the spore outer membrane in dormant and germinating spores of Bacillus megaterium | |
Barrabin et al. | Isolation and characterization of gyroxin from Crotalus durissus terrificus venom | |
Ohshita et al. | Analysis of lysosomal degradation of fluorescein isothiocyanate-labelled proteins by Toyopearl HW-40 affinity chromatography | |
Wagner et al. | Leucostoma peptidase A: isolation and physical properties | |
Uren | Affinity chromatography of proteolytic enzymes | |
Grushoff et al. | Purification and properties of streptococcal nicotinamide adenine dinucleotide glycohydrolase | |
Vanha-Perttula et al. | Cathepsin C activity as related to some histochemical substrates |