[go: up one dir, main page]

Brocklehurst et al., 1973 - Google Patents

Covalent chromatography. Preparation of fully active papain from dried papaya latex

Brocklehurst et al., 1973

View PDF
Document ID
15486688099610879342
Author
Brocklehurst K
Carlsson J
Kierstan M
Crook E
Publication year
Publication venue
Biochemical Journal

External Links

Snippet

1. A Sepharose–(glutathione–2-pyridyl disulphide) conjugate has been prepared. 2. Its use in a new type of chromatography, covalent chromatography by thiol–disulphide interchange, is described. 3. With this technique, papain containing 1 intact catalytic site [thiol with high …
Continue reading at pmc.ncbi.nlm.nih.gov (PDF) (other versions)

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells being immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells being immobilised on or in an organic carrier
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y10S435/815—Enzyme separation or purification by sorption

Similar Documents

Publication Publication Date Title
Brocklehurst et al. Covalent chromatography. Preparation of fully active papain from dried papaya latex
Brocklehurst et al. [66] Covalent chromatography by thiol-disulfide interchange
Cuatrecasas et al. Selective enzyme purification by affinity chromatography.
Kim et al. A rapid method for the purification of S-adenosylmethionine: protein-carboxyl O-methyltransferase by affinity chromatography
Nilsson et al. p‐Toluenesulfonyl chloride as an activating agent of agarose for the preparation of immobilized affinity ligands and proteins
Tanaka et al. Purification and properties of chlorophyllase from greened rye seedlings
Wilcox et al. Some aspects of the mechanism of complexation of red kidney bean. alpha.-amylase inhibitor and. alpha.-amylase
Soliday et al. Isolation and characterization of a cutinase from Fusarium roseum culmorum and its immunological comparison with cutinases from F. solani pisi
Funahashi et al. Preparation of three types of heparin-Sepharose and their binding activities to thrombin and antithrombin III
Eshdat et al. Chemical conversion of aspartic acid 52, a catalytic residue in hen egg-white lysozyme, to homoserine
Bodwell et al. Purification and characterization of carboxypeptidase A from rat skeletal muscle
EP0153763B1 (en) Affinity chromatography matrix with built-in reaction indicator
Ashley et al. Purification and properties of cytidine deaminase from escherichia coli.
US3794563A (en) Preparation of immobilized enzymes
Gurne et al. Dissociation and reassociation of immobilized porphobilinogen synthase: use of immobilized subunits for enzyme isolation.
Ramseyer et al. Purification of the cAMP receptor protein by affinity chromatography
Hurst et al. HYDROLYSIS OF CALF THYMUS DEOXYRIBONUCLEATE BY PANCREATIC DEOXYRIBONUCLEASE: I. A STUDY OF THE OLIGONUCLEOTIDES LIBERATED IN THE PRESENCE OF MAGNESIUM OR MANGANESE IONS
Ramseyer et al. The use of affinity chromatography in purification of cyclic nucleotide receptor proteins
Stults et al. Immobilization of proteins on partially hydrolyzed agarose beads
Dixon et al. Hydrophobic esters of cellulose: properties and applications in biochemical technology
Eun et al. Reaction of phenylglyoxal and {p-hydroxyphenyl} glyoxal with arginines and cysteines in the. alpha. subunit of tryptophan synthase
Harper et al. Studies on the mechanism of action of collagenase: Inhibition by cysteine and other chelating agents
Ikeda et al. Preparation and characterization of several new immobilized derivatives of pyridoxal 5′-phosphate
Pacaud Purification of protease II from Escherichia coli by affinity chromatography and separation of two enzyme species from cells harvested at late log phase
EP0184361B1 (en) Method of activating polymeric carrier