Wannlund et al., 1983 - Google Patents
[33] Bioluminescent immunoassaysWannlund et al., 1983
- Document ID
- 14099984151297384083
- Author
- Wannlund J
- DeLuca M
- Publication year
- Publication venue
- Methods in enzymology
External Links
Snippet
The procedures described here have been developed for only a few antigens at present. It seems very likely that they can be extended to any other antigen of choice. For sensitivities in the picomole range, the luciferase-antigen procedure is satisfactory. For increased …
- 239000000427 antigen 0 abstract description 33
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/535—Production of labelled immunochemicals with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay
- G01N33/543—Immunoassay; Biospecific binding assay with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/972—Modified antibody, e.g. hybrid, bifunctional
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/978—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/975—Kit
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/815—Test for named compound or class of compounds
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Kato et al. | Enzyme‐Linked Immunoassay: Conjugation of Rabbit Anti‐(Human Immunoglobulin G) Antibody with β‐d‐Galactosidase from Escherichia coli and Its Use for Human Immunoglobulin G Assay | |
Kato et al. | Enzyme-linked immunoassay: Conjugation of the Fab′ fragment of rabbit IgG with β-d-galactosidase from E. coli and its use for immunoassay | |
Nakane et al. | Peroxidase-labeled antibody a new method of conjugation | |
EP0365685B1 (en) | Freeze-dried composition comprising a horseradish peroxidase-labelled Fab' fragment of an anti-human interferon-beta monclonal antibody and trehalose; EIA kit containing the composition | |
EP0175560A2 (en) | Immunoassays utilizing covalent conjugates of polymerized enzyme and antibody | |
Vlasenko et al. | An investigation on the catalytic mechanism of enhanced chemiluminescence: immunochemical applications of this reaction | |
US5643731A (en) | Use of pairs of leucine zipper peptides in immunoassay methods | |
US4375972A (en) | Heterogeneous chemiluminescent immunoassays utilizing metallo porphyrin tag | |
EP0532187A1 (en) | Homogeneous immunoassays using enzyme inhibitors | |
Freytag et al. | A highly sensitive affinity-column-mediated immunometric assay, as exemplified by digoxin. | |
Wannlund et al. | A sensitive bioluminescent immunoassay for dinitrophenol and trinitrotoluene | |
EP0062632A1 (en) | Steric hindrance enzyme immunoassay | |
Cox et al. | Sensitive amplified immunoenzymometric assays (IEMA) for human insulin and intact proinsulin | |
CA1194415A (en) | Immunoglobulin half-molecules and process for producing hybrid antibodies | |
CA1135620A (en) | Immunological compound attached to metallo-porphyrin tag | |
Seto et al. | Development of ultra‐high sensitivity bioluminescent enzyme immunoassay for prostate‐specific antigen (PSA) using firefly luciferase | |
Wannlund et al. | [33] Bioluminescent immunoassays | |
US5302533A (en) | Antibody-enhanced chemiluminescence reactions | |
Jablonski | The preparation of bacterial luciferase conjugates for immunoassay and application to rubella antibody detection | |
Ikariyama et al. | Solid-phase luminescent catalyst immunoassay for human serum albumin with hemin as labeling catalyst | |
Bachas et al. | Cooperative interaction of immobilized folate binding protein with enzyme-folate conjugates: an enzyme-linked assay for folate | |
EP0353895A1 (en) | Immunoassay method | |
Brontman et al. | Homogeneous enzyme-linked assays mediated by enzyme antibodies; a new approach to electrode-based immunoassays | |
US4812395A (en) | Method for detecting enzymatic activity using particle agglutination | |
IMAGAWA et al. | Preparation of a monomeric 2, 4-dinitrophenyl Fab′-β-D-galactosidase conjugate for immunoenzymometric assay |