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Guo et al., 2018 - Google Patents

'Cold shock'increases the frequency of homology directed repair gene editing in induced pluripotent stem cells

Guo et al., 2018

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Document ID
1324328195260431606
Author
Guo Q
Mintier G
Ma-Edmonds M
Storton D
Wang X
Xiao X
Kienzle B
Zhao D
Feder J
Publication year
Publication venue
Scientific reports

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Snippet

Using CRISPR/Cas9 delivered as a RNA modality in conjunction with a lipid specifically formulated for large RNA molecules, we demonstrate that homology directed repair (HDR) rates between 20–40% can be achieved in induced pluripotent stem cells (iPSC) …
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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