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Holmes et al., 1997 - Google Patents

Purification and analysis of an extremely halophilic β-galactosidase from Haloferax alicantei

Holmes et al., 1997

Document ID
11436612564482343166
Author
Holmes M
Scopes R
Moritz R
Simpson R
Englert C
Pfeifer F
Dyall-Smith M
Publication year
Publication venue
Biochimica et Biophysica Acta (BBA)-Protein Structure and Molecular Enzymology

External Links

Snippet

As a first step in the development of a reporter system for gene expression in halophilic archaea, a β-galactosidase was purified 140-fold from Haloferax alicantei (previously phenon K, strain Aa2. 2). An overproducing mutant was first isolated by UV mutagenesis and …
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2468—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1) acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81)
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