KANAOKA et al., 1985 - Google Patents
New fluorogenic substrates for subtilisinKANAOKA et al., 1985
View PDF- Document ID
- 10635098701678836975
- Author
- KANAOKA Y
- TAKAHASHI T
- NAKAYAMA H
- TANIZAWA K
- Publication year
- Publication venue
- Chemical and pharmaceutical bulletin
External Links
Snippet
抄録 Two 7-amino-4-methylcoumarinamide derivatives of tripeptides were synthesized. They were shown to be potentially useful substrates for fluorometric microdetermination of subtilisins BPN'and Carlsberg. The kinetic characteristics of the hydrolyses of these …
- 108090000787 Subtilisin 0 title description 8
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases Endopeptidases (3.4.21-3.4.25) derived from animal tissue
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/914—Hydrolases (3)
- G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
- G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
- G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
- G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
- G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP0046742B1 (en) | Peptide substrates for determination of protease activity | |
| US5871938A (en) | Dioxetane compounds for the chemiluminescent detection of proteases, methods of use and kits therefore | |
| Fujiwara et al. | New chromogenic and fluorogenic substrates for pyrrolidonyl peptidase | |
| US4275153A (en) | Analytical fluorogenic substrates for proteolytic enzymes | |
| CA1161432A (en) | Tripeptide derivatives and their application in assaying enzymes | |
| Castillo et al. | Sensitive substrates for human leukocyte and porcine pancreatic elastase: a study of the merits of various chromophoric and fluorogenic leaving groups in assays for serine proteases | |
| US4640893A (en) | Novel rhodamine derivatives as fluorogenic substrates for proteinases | |
| US5098828A (en) | D-luciferin derivatives, their application and processes for the detection of ligands marked with an enzyme in the determination of biochemical substances | |
| EP0004256A1 (en) | Easily split substrates for the quantification of proteases, a process for their production and a method for the quantification of proteases | |
| DK149333B (en) | Tripeptide AND SALTS OF MINERAL organic acids FOR USE AS SUBSTRATES FOR DETERMINATION OF thrombin and thrombin-like ENZYMES ECARINTHROMBIN, plasmin and plasmin ENZYMES AND proenzymes PROENZYMAKTIVATORER, and enzyme inhibitors PURPOSE In LEGEMSVAESKERFRA PEOPLE AND MAMMALS AND IN animal CELL EXTRACTS | |
| Monsees et al. | Synthesis and Characterization of a Bioluminogenic Substrate for α-Chymotrypsin | |
| Rasnick et al. | Active site directed irreversible inhibition of thermolysin | |
| US4336186A (en) | Analytical fluorogenic substrates for proteolytic enzymes | |
| EP0076042B1 (en) | Novel substrates for measuring thrombin | |
| Pierzchala et al. | A new fluorogenic substrate for plasmin | |
| Johansen et al. | Colorimetric and fluorimetric microplate assays for legumain and a staining reaction for detection of the enzyme after electrophoresis | |
| KANAOKA et al. | New fluorogenic substrates for subtilisin | |
| Brynes et al. | 6-Aminoquinoline as a fluorogenic leaving group in peptide cleavage reactions: a new fluorogenic substrate for chymotrypsin | |
| Clavin et al. | Use of peptidyl-4-methoxy-2-naphthylamides to assay plasmin | |
| Filippova et al. | Fluorogenic peptide substrates for assay of aspartyl proteinases | |
| Bang et al. | Sensitivity and specificity of plasma serine protease chromogenic substrates | |
| Brown et al. | Sensitive fluorescent determination of trypsin-like proteases | |
| TSUNEMATSU et al. | Kinetics of Hydrolysis of Amide and Anilide Substrates of p-Guanidine-L-Phenylalanine by Bovine and Porcine Trypsins | |
| TANIZAWA | New F luorogenic Substrates for Subtilisinl | |
| WO2010090756A2 (en) | Fret-assays, methods for performing the assays and compounds relevant to the assays |