Xu et al., 1996 - Google Patents
Separation of basic proteins by capillary zone electrophoresis with coatings of a copolymer of vinylpyrrolidone and vinylimidazoleXu et al., 1996
- Document ID
- 10028401139101255391
- Author
- Xu R
- Vidal-Madjar C
- Sébille B
- Diez-Masa J
- Publication year
- Publication venue
- Journal of Chromatography A
External Links
Snippet
Capillary zone electrophoretic (CZE) separation of basic proteins has been achieved with capillary columns modified with copolymers of vinylpyrrolidone (VP) and vinylimidazole (VI). The copolymerization reaction is performed inside the capillary column and involves …
- 102000004169 proteins and genes 0 title abstract description 49
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44752—Controlling the zeta potential, e.g. by wall coatings
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44743—Introducing samples
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44708—Cooling
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electro-chemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/42—Measuring disposition or liberation of materials from an electrolyte; Coulometry, i.e. measuring coulomb-equivalent of material in an electrolyte
- G01N27/423—Coulometry
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Albarghouthi et al. | Poly‐N‐hydroxyethylacrylamide as a novel, adsorbed coating for protein separation by capillary electrophoresis | |
| McCormick | Capillary zone electrophoretic separation of peptides and proteins using low pH buffers in modified silica capillaries | |
| Bruin et al. | Capillary zone electrophoretic separations of proteins in polyethylene glycol-modified capillaries | |
| US4931328A (en) | Capillary tube with reduced protein interactions and controllable electroosmotic flow | |
| Xu et al. | Separation of basic proteins by capillary zone electrophoresis with coatings of a copolymer of vinylpyrrolidone and vinylimidazole | |
| US5085756A (en) | Column separation system for electrophoresis with sample pretreatment | |
| Liu et al. | Poly (diallyldimethylammonium chloride) as a Cationic Coating for Capillary Electrophoresis | |
| US5262031A (en) | Electroosmotic flow control apparatus for capillary electrophoresis | |
| Kohr et al. | Capillary electrophoresis with surface coated capillaries | |
| Huang et al. | Hydrolytically stable cellulose‐derivative coatings for capillary electrophoresis of peptides, proteins and glycoconjugates | |
| Yao et al. | Manipulation of electroosmotic flow in capillary electophoresis | |
| Guo et al. | Hydrolytically stable amino-silica glass coating material for manipulation of the electroosmotic flow in capillary electrophoresis | |
| EP0494544A2 (en) | Deactivated capillary columns for use in electrophoresis and micellar electrokinetic chromatography | |
| US5089103A (en) | Electrophoresis capillary with agarose | |
| Cifuentes et al. | Separation of basic proteins by capillary electrophoresis using cross-linked polyacrylamide-coated capillaries and cationic buffer additives | |
| US5114768A (en) | Surfaces with reduced protein interactions | |
| US5006313A (en) | Halogenated surface with reduced protein interaction | |
| Ren et al. | Poly (ethylene-propylene glycol)-modified fused-silica columns for capillary electrophoresis using epoxy resin as intermediate coating | |
| GB2276244A (en) | Capillary electrophoresis tube with reversible protein interaction coating | |
| Huang et al. | Charged surface coatings for capillary electrophoresis | |
| JP3399593B2 (en) | Method for inactivating the inner surface of a capillary | |
| Zhang et al. | Enantiomeric separation by capillary electrochromatography I. Chiral separation of dansyl amino acids and organochlorine pesticides on a diol‐silica dynamically coated with hydroxypropyl‐β‐cyclodextrin | |
| Nashabeh et al. | Fundamental and practical aspects of coupled capillaries for the control of electroosmotic flow in capillary zone electrophoresis of proteins | |
| Capelli et al. | ‘Tunable’positive and negative surface charges on a capillary wall: exploiting the Immobiline chemistry | |
| KR20010072246A (en) | Surfaces with reduced electroosmotic flow |