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ZA875912B - Immortalisation by dna transfer - Google Patents

Immortalisation by dna transfer

Info

Publication number
ZA875912B
ZA875912B ZA875912A ZA875912A ZA875912B ZA 875912 B ZA875912 B ZA 875912B ZA 875912 A ZA875912 A ZA 875912A ZA 875912 A ZA875912 A ZA 875912A ZA 875912 B ZA875912 B ZA 875912B
Authority
ZA
South Africa
Prior art keywords
immortalisation
cell
dna transfer
human
animal
Prior art date
Application number
ZA875912A
Inventor
Willecke Klaus
Johann Abken Hinrich
Jungfer Heinrich Barchet Herbert
Original Assignee
Boehringer Mannheim Gmbh.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim Gmbh. filed Critical Boehringer Mannheim Gmbh.
Publication of ZA875912B publication Critical patent/ZA875912B/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

To immortalise human or animal cells by fusion with a non-replicable fragment of a cell which can be permanently cultured, and cultivation thereof in a culture medium without selection substances, the cell to be immortalised is used to transfect DNA obtained from the cytoplasm of a human or animal cell which can be permanently cultured.
ZA875912A 1986-08-12 1987-08-11 Immortalisation by dna transfer ZA875912B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19863627326 DE3627326A1 (en) 1986-08-12 1986-08-12 IMMORTALIZATION THROUGH DNS TRANSMISSION

Publications (1)

Publication Number Publication Date
ZA875912B true ZA875912B (en) 1988-02-12

Family

ID=6307220

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA875912A ZA875912B (en) 1986-08-12 1987-08-11 Immortalisation by dna transfer

Country Status (7)

Country Link
EP (1) EP0256512B1 (en)
JP (1) JPH074237B2 (en)
AT (1) ATE87653T1 (en)
AU (1) AU581819B2 (en)
CA (1) CA1313832C (en)
DE (2) DE3627326A1 (en)
ZA (1) ZA875912B (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ226750A (en) * 1987-10-29 1990-09-26 Amrad Corp Ltd Immortalisation of neural precursor cells by introducing a retrovirus vector containing a myc-oncogene
WO1989005862A1 (en) * 1987-12-09 1989-06-29 Invitron Corporation Human cell life extension
DE4218945A1 (en) * 1992-06-10 1993-12-16 Boehringer Mannheim Gmbh DNA for immortalizing human or animal cells
AU6415396A (en) * 1995-06-23 1997-01-22 Micromet Gmbh Immortalized epithelial tumor cell
JP2002201138A (en) * 2000-12-28 2002-07-16 Japan Tissue Engineering:Kk Neovascularization inhibitor

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3245665A1 (en) * 1982-05-04 1983-11-10 Boehringer Mannheim Gmbh, 6800 Mannheim METHOD FOR PRODUCING PERMANENT ANIMAL AND HUMAN CELL LINES AND THE USE THEREOF
US4652522A (en) * 1983-07-05 1987-03-24 The University Of Pennsylvania Continuous lymphocyte cell lines, their production and use
EP0207147A4 (en) * 1984-12-21 1988-07-14 Techniclone Res Partners Method for electrically immortalizing lymphoid cells.

Also Published As

Publication number Publication date
AU7677187A (en) 1988-02-18
AU581819B2 (en) 1989-03-02
ATE87653T1 (en) 1993-04-15
EP0256512A3 (en) 1989-12-06
JPH074237B2 (en) 1995-01-25
EP0256512B1 (en) 1993-03-31
EP0256512A2 (en) 1988-02-24
DE3785109D1 (en) 1993-05-06
JPS6356283A (en) 1988-03-10
CA1313832C (en) 1993-02-23
DE3627326A1 (en) 1988-02-18

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