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ZA200308254B - Circuit arrangement for injecting nucleic acids and other biologically active molecules into the nucleus of higher educaryontic cells using electrical currents. - Google Patents

Circuit arrangement for injecting nucleic acids and other biologically active molecules into the nucleus of higher educaryontic cells using electrical currents.

Info

Publication number
ZA200308254B
ZA200308254B ZA200308254A ZA200308254A ZA200308254B ZA 200308254 B ZA200308254 B ZA 200308254B ZA 200308254 A ZA200308254 A ZA 200308254A ZA 200308254 A ZA200308254 A ZA 200308254A ZA 200308254 B ZA200308254 B ZA 200308254B
Authority
ZA
South Africa
Prior art keywords
nucleic acids
biologically active
active molecules
educaryontic
nucleus
Prior art date
Application number
ZA200308254A
Other languages
English (en)
Inventor
Gudula Riemen
Juliana Helfrich
Meike Weigel
Heike Wessendorf
Corinna Thiel
Gregor Siebenkotten
Rothmann Kirste Mueller-Hartma
Rainer Christine
Elke Lorbach
Original Assignee
Amaxa Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amaxa Gmbh filed Critical Amaxa Gmbh
Publication of ZA200308254B publication Critical patent/ZA200308254B/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/18—Applying electric currents by contact electrodes
    • A61N1/32—Applying electric currents by contact electrodes alternating or intermittent currents
    • A61N1/327—Applying electric currents by contact electrodes alternating or intermittent currents for enhancing the absorption properties of tissue, e.g. by electroporation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/02—Details
    • A61N1/04—Electrodes
    • A61N1/0404—Electrodes for external use
    • A61N1/0408—Use-related aspects
    • A61N1/0412—Specially adapted for transcutaneous electroporation, e.g. including drug reservoirs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • H—ELECTRICITY
    • H03—ELECTRONIC CIRCUITRY
    • H03K—PULSE TECHNIQUE
    • H03K3/00—Circuits for generating electric pulses; Monostable, bistable or multistable circuits
    • H03K3/02—Generators characterised by the type of circuit or by the means used for producing pulses
    • H03K3/53—Generators characterised by the type of circuit or by the means used for producing pulses by the use of an energy-accumulating element discharged through the load by a switching device controlled by an external signal and not incorporating positive feedback
    • H03K3/57—Generators characterised by the type of circuit or by the means used for producing pulses by the use of an energy-accumulating element discharged through the load by a switching device controlled by an external signal and not incorporating positive feedback the switching device being a semiconductor device

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Radiology & Medical Imaging (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
ZA200308254A 2001-04-23 2003-10-23 Circuit arrangement for injecting nucleic acids and other biologically active molecules into the nucleus of higher educaryontic cells using electrical currents. ZA200308254B (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE10119901A DE10119901A1 (de) 2001-04-23 2001-04-23 Schaltungsanordnung zur Einbringung von Nukleinsäuren und anderen biologisch aktiven Molekülen in den Kern höherer eukaryontischer Zellen mit Hilfe elektrischen Stroms

Publications (1)

Publication Number Publication Date
ZA200308254B true ZA200308254B (en) 2004-05-06

Family

ID=7682433

Family Applications (1)

Application Number Title Priority Date Filing Date
ZA200308254A ZA200308254B (en) 2001-04-23 2003-10-23 Circuit arrangement for injecting nucleic acids and other biologically active molecules into the nucleus of higher educaryontic cells using electrical currents.

Country Status (15)

Country Link
US (2) US8173416B2 (xx)
EP (2) EP1383901B1 (xx)
JP (2) JP4124659B2 (xx)
KR (1) KR100810173B1 (xx)
CN (1) CN1257979C (xx)
AT (2) ATE391185T1 (xx)
AU (1) AU2007202279B2 (xx)
CA (1) CA2445257C (xx)
DE (4) DE10119901A1 (xx)
ES (2) ES2305642T3 (xx)
IL (2) IL158513A0 (xx)
MX (1) MXPA03009666A (xx)
NO (1) NO20034681L (xx)
WO (1) WO2002086129A1 (xx)
ZA (1) ZA200308254B (xx)

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US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
WO2007081631A2 (en) * 2006-01-04 2007-07-19 Healtheuniverse, Inc. Method of producing insulin-secreting cells from adult stem cells via transfection
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US9213999B2 (en) 2007-06-15 2015-12-15 Kyoto University Providing iPSCs to a customer
JP2008307007A (ja) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
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ES2722198T3 (es) 2008-05-02 2019-08-08 Univ Kyoto Método de reprogramación nuclear
EP2208778A1 (de) 2009-01-20 2010-07-21 Lonza Cologne AG Verfahren und Vorrichtung zur elektrischen Behandlung von Reaktionsräumen
US20140099284A1 (en) 2010-10-15 2014-04-10 Eos Neuroscience, Inc Modulation neural pathways
WO2012098260A1 (en) 2011-01-21 2012-07-26 Axiogenesis Ag A non-viral system for the generation of induced pluripotent stem (ips) cells
US9452199B2 (en) 2014-01-17 2016-09-27 General Electric Company Platelet activation and growth factor release using electric pulses
US10421956B2 (en) 2014-01-17 2019-09-24 General Electric Company Electric pulse generation systems using capacitive coupling
US9708597B2 (en) * 2014-01-17 2017-07-18 General Electric Company Electric pulse generation systems using capacitive coupling
US10093706B2 (en) 2017-01-30 2018-10-09 Indiana University Research And Technology Corporation Dominant positive hnRNP-E1 polypeptide compositions and methods
JP6518971B2 (ja) * 2017-05-12 2019-05-29 株式会社ベックス エレクトロポレーター用電源
US10968423B2 (en) 2018-02-26 2021-04-06 General Electric Company System and method for electric pulse based activation of biological samples
IL296677A (en) * 2020-03-26 2022-11-01 St Andrews Pharmaceutical Tech Ltd Apparatus for improved transfection efficiency and/or protein expression and method of use thereof

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Also Published As

Publication number Publication date
EP1522587A3 (de) 2005-06-08
JP2004536584A (ja) 2004-12-09
JP2008237214A (ja) 2008-10-09
IL158513A (en) 2010-04-15
ES2231709T3 (es) 2005-05-16
US20090023131A1 (en) 2009-01-22
EP1522587A2 (de) 2005-04-13
ES2305642T3 (es) 2008-11-01
DE10291733D2 (de) 2004-04-15
DE50212037D1 (de) 2008-05-15
KR100810173B1 (ko) 2008-03-06
DE10119901A1 (de) 2002-10-24
IL158513A0 (en) 2004-05-12
ATE391185T1 (de) 2008-04-15
AU2007202279A1 (en) 2007-06-07
JP4124659B2 (ja) 2008-07-23
EP1383901B1 (de) 2004-11-03
WO2002086129A1 (de) 2002-10-31
DE50201472D1 (de) 2004-12-09
US20040137603A1 (en) 2004-07-15
CA2445257C (en) 2014-10-28
AU2007202279B2 (en) 2010-10-28
NO20034681D0 (no) 2003-10-20
CN1257979C (zh) 2006-05-31
CA2445257A1 (en) 2002-10-31
ATE281529T1 (de) 2004-11-15
KR20040000437A (ko) 2004-01-03
NO20034681L (no) 2003-12-16
JP4694587B2 (ja) 2011-06-08
CN1503845A (zh) 2004-06-09
EP1522587B1 (de) 2008-04-02
EP1383901A1 (de) 2004-01-28
US8173416B2 (en) 2012-05-08
MXPA03009666A (es) 2004-12-06

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