[go: up one dir, main page]

WO2025049331A3 - Aptamer detection techniques - Google Patents

Aptamer detection techniques Download PDF

Info

Publication number
WO2025049331A3
WO2025049331A3 PCT/US2024/043704 US2024043704W WO2025049331A3 WO 2025049331 A3 WO2025049331 A3 WO 2025049331A3 US 2024043704 W US2024043704 W US 2024043704W WO 2025049331 A3 WO2025049331 A3 WO 2025049331A3
Authority
WO
WIPO (PCT)
Prior art keywords
detection techniques
aptamer
aptamer detection
sequencing library
library preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
PCT/US2024/043704
Other languages
French (fr)
Other versions
WO2025049331A2 (en
Inventor
Jason Betley
Steven Norberg
Anthony Chau
Colin Brown
Jesper Lindberg ENLUND
Niall Anthony Gormley
Daniel Uriguen BECKETT
Bryan ANSTEAD
Nithya Subramanian
Kuanlin WU
Andrew Slatter
Yang Cao
Carlo RANDISE-HINCHLIFF
M. Maggie O'MEARA
David Jones
Istvan KOCSIS
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Illumina Inc
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of WO2025049331A2 publication Critical patent/WO2025049331A2/en
Publication of WO2025049331A3 publication Critical patent/WO2025049331A3/en
Anticipated expiration legal-status Critical
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Aptamer detection techniques are provided that facilitate sequencing library preparation. In embodiments, reporter probes complementary to aptamers may be used to generate amplification products to form a sequencing library. In embodiments, the aptamer may be used as part of a library preparation.
PCT/US2024/043704 2023-08-31 2024-08-23 Aptamer detection techniques Pending WO2025049331A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202363535976P 2023-08-31 2023-08-31
US63/535,976 2023-08-31

Publications (2)

Publication Number Publication Date
WO2025049331A2 WO2025049331A2 (en) 2025-03-06
WO2025049331A3 true WO2025049331A3 (en) 2025-06-05

Family

ID=92791993

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/043704 Pending WO2025049331A2 (en) 2023-08-31 2024-08-23 Aptamer detection techniques

Country Status (1)

Country Link
WO (1) WO2025049331A2 (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5942609A (en) * 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support
US20160060687A1 (en) * 2014-07-18 2016-03-03 Cdi Laboratories, Inc. Methods and compositions to identify, quantify, and characterize target analytes and binding moieties
EP2076613B1 (en) * 2007-07-17 2016-09-07 Somalogic, Inc. Analyses of test samples
WO2019055985A1 (en) * 2017-09-18 2019-03-21 Eccrine Systems, Inc. Click chemistry aptamer tagging for eab biosensors
WO2022208171A1 (en) * 2021-03-31 2022-10-06 UCL Business Ltd. Methods for analyte detection
US20220348985A1 (en) * 2019-06-21 2022-11-03 Northwestern University Forced intercalation (fit)-aptamers: probes based on forced intercalation

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
EP3034626A1 (en) 1997-04-01 2016-06-22 Illumina Cambridge Limited Method of nucleic acid sequencing
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
EP2607369B1 (en) 2002-08-23 2015-09-23 Illumina Cambridge Limited Modified nucleotides for polynucleotide sequencing
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
JP2007525571A (en) 2004-01-07 2007-09-06 ソレクサ リミテッド Modified molecular array
WO2006064199A1 (en) 2004-12-13 2006-06-22 Solexa Limited Improved method of nucleotide detection
JP4990886B2 (en) 2005-05-10 2012-08-01 ソレックサ リミテッド Improved polymerase
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US7329860B2 (en) 2005-11-23 2008-02-12 Illumina, Inc. Confocal imaging methods and apparatus
EP3285067B1 (en) 2006-12-14 2022-06-22 Life Technologies Corporation Apparatus for measuring analytes using fet arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US7855054B2 (en) 2007-01-16 2010-12-21 Somalogic, Inc. Multiplexed analyses of test samples
US20110136099A1 (en) 2007-01-16 2011-06-09 Somalogic, Inc. Multiplexed Analyses of Test Samples
US8975026B2 (en) 2007-01-16 2015-03-10 Somalogic, Inc. Method for generating aptamers with improved off-rates
US7964356B2 (en) 2007-01-16 2011-06-21 Somalogic, Inc. Method for generating aptamers with improved off-rates
WO2008093098A2 (en) 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US8829171B2 (en) 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
EP2635679B1 (en) 2010-11-05 2017-04-19 Illumina, Inc. Linking sequence reads using paired code tags
WO2019059961A1 (en) 2017-09-25 2019-03-28 California Institute Of Technology Bistable polynucleotide devices for the sensing and quantification of molecular events
CA3134831A1 (en) 2019-04-05 2020-10-08 Claret Bioscience, Llc Methods and compositions for analyzing nucleic acid
JP2024521196A (en) 2021-05-28 2024-05-28 イルミナ インコーポレイテッド Oligonucleotide analogues for the preparation of nucleic acids

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5942609A (en) * 1998-11-12 1999-08-24 The Porkin-Elmer Corporation Ligation assembly and detection of polynucleotides on solid-support
EP2076613B1 (en) * 2007-07-17 2016-09-07 Somalogic, Inc. Analyses of test samples
US20160060687A1 (en) * 2014-07-18 2016-03-03 Cdi Laboratories, Inc. Methods and compositions to identify, quantify, and characterize target analytes and binding moieties
WO2019055985A1 (en) * 2017-09-18 2019-03-21 Eccrine Systems, Inc. Click chemistry aptamer tagging for eab biosensors
US20220348985A1 (en) * 2019-06-21 2022-11-03 Northwestern University Forced intercalation (fit)-aptamers: probes based on forced intercalation
WO2022208171A1 (en) * 2021-03-31 2022-10-06 UCL Business Ltd. Methods for analyte detection

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
LIAN FENGNAN ET AL: "A detection method of Escherichia coli O157:H7 based on immunomagnetic separation and aptamers-gold nanoparticle probe quenching Rhodamine B's fluorescence", FOOD SCIENCE AND BIOTECHNOLOGY, THE KOREA SOC. OF FOOD SCIENCE AND TECHNOLOGY, HEIDELBERG, vol. 30, no. 8, 28 July 2021 (2021-07-28), pages 1129 - 1138, XP037538583, ISSN: 1226-7708, [retrieved on 20210728], DOI: 10.1007/S10068-021-00947-3 *

Also Published As

Publication number Publication date
WO2025049331A2 (en) 2025-03-06

Similar Documents

Publication Publication Date Title
Flores et al. Physiological characterization of Saccharomyces uvarum and Saccharomyces eubayanus from Patagonia and their potential for cidermaking
Acker et al. Yeast RNA polymerase III transcription factors and effectors
MX2024010252A (en) Processes for reducing lactic acid in a biofuel fermentation system
MX2015009656A (en) Improved microalgal flour.
EP4372102A3 (en) Target reporter constructs and uses thereof
WO2016100955A3 (en) Compositions and methods for targeted depletion, enrichment, and partitioning of nucleic acids using crispr/cas system proteins
WO2015083002A3 (en) Multiplex detection of nucleic acids
WO2013013068A3 (en) Embodiments of a probe and method for targeting nucleic acids
MX2015004957A (en) Waxy oxidation products of rice bran.
WO2016168890A8 (en) Generation of muscle-lineage cells from stem cells
MX2022015233A (en) Production of desaturated fatty alcohols and desaturated fatty acyl acetates in yeast.
AU2016328448A8 (en) Fungal production of FDCA
BR112022012109A2 (en) YEAST CELL CAPABLE OF PRODUCING E8,E10-DODECADIENYL COENZYME A AND OPTIONAL E8,E10-DODECADIEN-1-OL, E, METHOD FOR PRODUCING E8,E10-DODECADIENYL COENZYME A AND OPTIONAL E8,E10-DODECADIEN-1-OL IN A YEAST CELL YEAST
MX344371B (en) Signal amplification.
MX2014014811A (en) Edible materials and their manufacture.
WO2016027162A3 (en) Methods and compositions for nucleic acid detection
CA3077390A1 (en) Methods and compositions for nucleic acid detection
WO2025049331A3 (en) Aptamer detection techniques
BR112018068855A2 (en) powders and tablets comprising omega-3 fatty acid derivatives and methods of their production
WO2015120542A8 (en) Hybrid proteins and uses thereof
CY1126064T1 (en) METHOD FOR THE PREPARATION OF 3-HYDROXYCARBONIC ACIDS BASED ON POLYOLS
MX386546B (en) Production of manool
EP4287845A4 (en) FREEZE-DRIED READY-TO-USE FOODS AND METHODS FOR THEIR PRODUCTION
WO2015077443A3 (en) Methods for producing dicarboxylic acids
BR112018000682A2 (en) Process for the preparation of a fermented milk product

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 24772120

Country of ref document: EP

Kind code of ref document: A2