WO2025003875A1

WO2025003875A1 - Ternary composition of spiromesifen, hexythiazox and abamectin

Info

Publication number: WO2025003875A1
Application number: PCT/IB2024/056153
Authority: WO
Inventors: Riya Nagpal
Original assignee: Riya Nagpal
Priority date: 2023-06-29
Filing date: 2024-06-25
Publication date: 2025-01-02

Abstract

The present invention relates to synergistic pesticidal composition for the protection of various crops, plants against pests including mites, whitefly and thrips, process of preparation and use thereof.

Description

FIELD OF THE INVENTION The present invention relates to synergistic pesticidal composition for the protection of various crops, plants against pests including mites, whitefly and thrips, process of preparation and use thereof. More particularly, the present invention relates to a synergistic pesticidal composition comprising A) Compound of Formula I, chemically known as 3-Mesityl-2-oxo-1- oxaspiro[4.4]non-3-en-4-yl) 3,3-dimethylbutyrate or its agrochemically acceptable salts, esters and derivatives; B) Hexythiazox or its agrochemically acceptable salts, esters and derivatives; and C) Abamectin or its agrochemically acceptable salts, esters and derivatives for synergistic and effective control of various pests including mites and thrips. The present invention also relates to a process for preparation of such pesticidal composition for synergistic and efficacious control of various pests including mites, whitefly and thrips. BACKGROUND OF THE INVENTION Pests are one of the worst enemies of crops. They cause severe damages to the crops resulting in reduced crop productivity. In heavily infested areas 80 to 90 percent crop damages has been reported. In agrarian countries like India, it badly impacts the economy of the country. Controlling crop pest population has been a major issue and various traditional and advanced means are being used to minimize the damage caused by various pests. In most cases, solo and binary formulations of insecticides and acaricides are used to control pests, particularly insects. Pest resistance, is the most major pest control problem in agriculture. The combination of various actives and auxiliaries is a common method for preventing and controlling resistant pests. Different actives are compounded, and the actual application effect determines whether the composition has synergism, addition, or antagonism. The biological properties of known compounds are not entirely satisfactory in the areas of pest control, phytotoxicity, environmental and worker exposure, for example, it has been observed that the pests become resistant to pesticides which are at times administered in higher dosages to achieve the desired control, thereby leading to soil toxicity and other environmental hazards besides higher costs. Thus, it is always preferable to be able to reduce the amount of chemical agents released into the environment while still ensuring effective pest control. Although various pest control agent combinations have been studied, a high synergistic action has not always been found. There is however a need for improvement of these combinations. There are various prior arts which disclose compositions comprising combination of pesticides including acaricides, insecticides having Abamectin combined with other insecticides, acaricides. Some of such prior arts are listed below: CN103621528 discloses a mite-killing composition, comprising an active component A and an active component B, wherein the active component A is Compound of Formula I, i.e.3-Mesityl- 2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3-dimethylbutyrate Technical, the active component B is any one compound selected from the following components: tebufenpyrad, Hexythiazox, fenazaquin and halfenprox, and the weight ratio of the active component A to the active component B is 1:70 to 70:1. CN101530075A relates to a micro-emulsion composition comprising Abamectin and Hexythiazox and its preparation method. CN1751572A discloses a mite-killing composition in the form of microemulsion, emulsified oil, solution, or suspension contains Abamectin and Hexythiazox in weight ratio of 1: (2-14). However, the biological properties of these mixtures of known compounds are not completely satisfactory in the field of pest control, phytotoxicity. In particular, it has been noted that pests are evolving a resistance to pesticides, which are frequently used at higher dosages to achieve the desired control, causing a risk to the environment and increasing costs. Hence, there is a need to develop a cost-effective synergistic composition which addresses the problem of resistance and soil toxicity and also is used at reduced dosages, controls environmental damage, offers broader crop protection, improved and healthy foliage, rainfastedness, improved crop yield, saves labour and control against various pests including insect pests, improves plant growth and is yet cost effective to the end user. Therefore, present invention aims to overcome the problems of the prior art and provide a synergistic pesticidal composition comprising bioactive amounts of A) compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3-dimethylbutyrate or its agrochemically acceptable salts, esters and derivatives; B) Hexythiazox or its agrochemically acceptable salts, esters and derivatives; and C) Abamectin or its agrochemically acceptable salts, esters and derivatives for synergistic and effective control of various pests including mites, whitefly and thrips.. OBJECTIVES OF THE INVENTION Accordingly, the main objective of the present invention is to provide a synergistic pesticidal composition for pest control of plants, crops including various types of mites, thrips and aphids. It is another objective of the present invention to provide improved, stable and ready to use pesticidal composition having synergistic and superior properties compared to prior art formulations or mixtures or compositions. It is yet another objective of the present invention to provide a pesticidal composition for synergistic and effective control of various types of mites, whitefly and thrips. Yet another objective of the present invention is to provide a process for the preparation of a stable synergistic pesticidal composition comprising A) compound of Formula I, chemically known as 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3-dimethylbutyrate or its agrochemically acceptable salts, esters and derivatives; B) Hexythiazox or its agrochemically acceptable salts, esters and derivatives; and C) Abamectin or its agrochemically acceptable salts, esters and derivatives for synergistic and effective control of various pests including mites and thrips. Yet another objective of the present invention is to provide a stable synergistic pesticidal composition that promotes plant health, growth and increases plant, crop yield in the field. Yet another objective of the present invention is to provide a composition and method for controlling various mites, whitefly and thrips in plants, crops and plant propagation materials. Another objective of the present invention is to provide one shot application of novel and effective synergistic pesticidal composition for killing/ controlling highly resistant mites, whitefly and thrips at lower application rates. It is another objective of the present invention to provide a novel and effective synergistic pesticidal composition which is environmentally safe, possesses broad spectrum bio-efficacy, is non-phytotoxic. It is yet another object of the present invention to provide a novel and effective synergistic pesticidal composition, which has improved stability during the desired shelf life at various temperature and pH conditions. It is another objective of the present invention to provide a novel and effective stable synergistic pesticidal composition, which is economically more beneficial. Advantages of the Present Invention: 1. The synergistic combination of the present invention resulted in significantly lower incidence of mites in various crops including Tea as compared to the other treatments and untreated check. 2. The synergistic combination of the present invention is significantly superior and synergistic from its individual product specifically manufactured by inventor at equal dose or readily available in market in controlling the mite incidence after first and second spray in various crops including Tea. 3. The synergistic combination of the present invention provides higher marketable green leaf yields as compared to the untreated control and binary and solo treatments applied in the various crops including Tea. Also found economical with higher Cost: Benefit Ratio. 4. The synergistic combination shows no phyto-toxicity effect on various crops including Tea. 5. The results of experiments indicate that the synergistic combination is highly effective in controlling insects and increasing production of fruits including for Tea crop at lower doses of the products in the combination, thus the invention will be cost effective and safer to the environment. SUMMARY OF THE INVENTION Accordingly, the present invention relates to a synergistic pesticidal composition comprising: A) compound of Formula I, i.e.3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3-dimethylbutyrate or its agrochemically acceptable salts, esters and derivatives; B) Hexythiazox or its agrochemically acceptable salts, esters and derivatives; and C) Abamectin or its agrochemically acceptable salts, esters and derivatives for synergistic and effective control of various pests including mites and thrips. In one embodiment of the present invention, the synergistic pesticidal composition comprises: a) compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate or its agrochemically acceptable salts, esters and derivatives; b) Hexythiazox or its agrochemically acceptable salts, esters and derivatives; c) Abamectin or its agrochemically acceptable salts, esters and derivatives and In yet another embodiment of the present invention, the synergistic pesticidal composition comprises: a) compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate or its agrochemically acceptable salts, esters and derivatives; b) Hexythiazox or its agrochemically acceptable salts, esters and derivatives; c) Abamectin or its agrochemically acceptable salts, esters and derivatives and d) One or more agrochemically acceptable excipients. In another embodiment of the present invention, the synergistic pesticidal composition comprises: a) compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate or its agrochemically acceptable salts, esters and derivatives, in the range of 0.1-30% by weight of the composition; b) Hexythiazox or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-10% by weight of the composition; and c) Abamectin or its agrochemically acceptable salts, esters and derivatives in the range of 0.1- 10% by weight of the composition In one another embodiment of the present invention, the synergistic pesticidal composition comprises: a) compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate or its agrochemically acceptable salts, esters and derivatives, in the range of 0.1-30% by weight of the composition; b) Hexythiazox or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-10% by weight of the composition; c) Abamectin or its agrochemically acceptable salts, esters and derivatives in the range of 0.1- 10% by weight of the composition; and d) One or more agrochemically acceptable excipients. In one embodiment of the present invention, the present pesticidal composition is stable and has enhanced shelf life. DETAILED DESCRIPTION OF THE INVENTION In describing the embodiments of the invention, specific terminology is resorted for sake of clarity. However, it is not intended that the invention be limited to specific terms so selected and it is to be understood that each specific term includes all technical equivalents that operate in a similar manner to accomplish a similar purpose. It has been observed by the inventors of the present invention that the composition of present invention not only has enhances bio-efficacy and provides synergistic effect but also gives control of pests for a longer duration as compared to solo and binary formulations of insecticides and acaricides. Compound of Formula I, i.e.3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3-dimethylbutyrate is an insecticide belongs to the class of tetronic acid. It is non-systemic and inhibits acetyl CoA carboxylase and is used for controlling whiteflies and mites including the two-spotted spidermite, grass mite, broad mite on fruits, vegetables and ornamental plants. The mechanism of action of the mite-killing agent is to influence the development of whiteflies and mites, interfere the biosynthesis of liposome of the whiteflies and particularly have good activity on larva stages, and simultaneously can generate the closing action of ovarian ducts, reduce the reproductive capacity of adult mites and whiteflies and greatly reduce the number of eggs laid. Indoor and field experiments prove that the it is safe to beneficial organisms, is suitable for comprehensive control of pests, has excellent residual effect, good plant compatibility and is safe to the environment. The structure is as shown below:

Hexythiazox is a thiazolidine insecticide with high activity. It is used for the control of eggs and larvae of many mites on a wide variety of food crops. The contact killing effect is strong, and the plant epidermal penetration is good, but the systemic conduction effect is not generated. The insecticidal action is very selective. It has good effect on young mites, nymphs and eggs of various tetranychids, has poor effect on adult mites, but has the effect of inhibiting hatching of eggs laid by female adult mites contacting with the medicament. It is non- systemic with contact and stomach action. It is a mite growth inhibitor affecting CHS1. The structure of Hexythiazox is as shown below: Abamectin is an insecticide belongs to the class of avermectin. It has a low aqueous solubility and is not volatile. It does not tend to be persistent in the environment. The action mechanism is to stimulate the release of a nerve transmission medium, namely r-aminobutyric acid, and interfere normal neurophysiological activities. This insecticide works by binding to glutamate-gated chloride channels in the nerve cells of pests, leading to an increase in chloride ion influx. Its systemic and translaminar properties allow it to move within plant tissues, providing extended protection against pests that feed on treated vegetation. Abamectin is adsorbed by soil in soil and cannot move and is decomposed by microorganisms, so that the abamectin has no accumulation effect in the environment and can be used as a component of comprehensive control. It mainly controls phytiphagus mites, leafminers, cockroachers, ants, pear psylla, nematodes including Meloidogyne species. It is used in wide variety of crops. It is used to kill insect pests in ornamental plants; fruit including citrus, pears, tomatoes, watermelon; Cotton; vegetable crops including eggplant, curcubits, beans. The structure of Abamectin is as shown below:

In one embodiment of the present invention, the synergistic pesticidal composition comprises: 9 a) compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate or its agrochemically acceptable salts, esters and derivatives; b) Hexythiazox or its agrochemically acceptable salts, esters and derivatives; and c) Abamectin or its agrochemically acceptable salts, esters and derivatives In yet another embodiment of the present invention, the synergistic pesticidal composition comprises: a) compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate or its agrochemically acceptable salts, esters and derivatives; b) Hexythiazox or its agrochemically acceptable salts, esters and derivatives; c) Abamectin or its agrochemically acceptable salts, esters and derivatives; and d) One or more agrochemically acceptable excipients. In another embodiment of the present invention, the synergistic pesticidal composition comprises: a) compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate or its agrochemically acceptable salts, esters and derivatives, in the range of 0.1-30% by weight of the composition; b) Hexythiazox or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-10% by weight of the composition; and c) Abamectin or its agrochemically acceptable salts, esters and derivatives in the range of 0.1- 10% by weight of the composition In one another embodiment of the present invention, the synergistic pesticidal composition comprises: a) compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate or its agrochemically acceptable salts, esters and derivatives, in the range of 0.1-30% by weight of the composition; b) Hexythiazox or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-10% by weight of the composition; c) Abamectin or its agrochemically acceptable salts, esters and derivatives in the range of 0.1- 10% by weight of the composition; and d) One or more agrochemically acceptable excipients. In yet another embodiment of the present invention, the synergistic pesticidal composition comprises: a) compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate or its agrochemically acceptable salts, esters and derivatives as 15% by weight of the composition; b) Hexythiazox or its agrochemically acceptable salts, esters and derivatives as 2.2% by weight of the composition; and c) Abamectin or its agrochemically acceptable salts, esters and derivatives as 1.15% by weight of the composition. In yet another embodiment of the present invention, the synergistic pesticidal composition comprises: a) compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate or its agrochemically acceptable salts, esters and derivatives as 20 % by weight of the composition; b) Hexythiazox or its agrochemically acceptable salts, esters and derivatives as 3.0 % by weight of the composition; and c) Abamectin or its agrochemically acceptable salts, esters and derivatives as 0.50% by weight of the composition In yet another embodiment of the present invention, the synergistic pesticidal composition comprises: a) compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate or its agrochemically acceptable salts, esters and derivatives as 18 % by weight of the composition; b) Hexythiazox or its agrochemically acceptable salts, esters and derivatives as 5.0 % by weight of the composition; and c) Abamectin or its agrochemically acceptable salts, esters and derivatives as 1.25% by weight of the composition. In another embodiment of the present invention, it is provided a process for preparation of the synergistic pesticidal composition comprising: a) compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate or its agrochemically acceptable salts, esters and derivatives, in the range of 0.1-30% by weight of the composition; b) Hexythiazox or its agrochemically acceptable salts, esters and derivatives in the range of 0.1-10% by weight of the composition; and c) Abamectin or its agrochemically acceptable salts, esters and derivatives in the range of 0.1- 10% by weight of the composition In accordance with an embodiment of the present invention, there is provided a synergistic pesticidal composition comprising active ingredients present in the weight % range as given below: Compound of Formula I, i.e. 3-Mesityl-2-oxo-1- Hexythiazox or its Abamectin or its oxaspiro[4.4]non-3-en-4-yl) agrochemically agrochemically acceptable 3,3-dimethylbutyrate or its acceptable salts, esters salts, esters and derivatives agrochemically acceptable and derivatives salts, esters and derivatives 0.1-30% 0.1-10% 0.1-10% In one aspect/ another embodiment, the pesticidal composition of present invention can be applied to a plant/crop/soil by spraying, rubbing, atomizing, irrigating, evaporating, dusting, fogging, broadcasting, pouring, mist blowing, soil mixing, foaming, painting, spreading-on, drenching, dipping or drip irrigation. etc. In yet another embodiment of the present invention, the present pesticidal composition can be applied to various crops and plants including Apple, Chilli, Tea, Cotton, Rose (Ornamental), Grapes, wheat, soyabean, rice, ground nut, pulses including black gram, paddy and vegetables including but not limited to okra, tomato, Brinjal, citrus, sugar beet, pears, egg-plants, lettuce, iceberg lettuce, pepper, cucumber, squash, melon, bean, dry-beans, peas, leek, garlic, onion, cabbage, carrot, cluster bean , tuber such as potato, sugar cane, tobacco, watermelon ,coffee, turf and forage, cruciferous, cucurbits, grapevines, tea, pepper, fodder beet, oil seed rape, pansy, impatiens, petunia, menthe, jute and geranium, etc In yet another embodiment of the present invention, the present pesticidal composition provides synergistic and effective control of various pests including thrips, white flies and mites such as Red spider mites (Tetranychus urticae), Chilli Yellow Mite, Scarlet mite, European Red Mite etc. In yet another embodiment of the present invention, the present pesticidal composition can be formulated as one or more of Capsule suspension (CS), Dispersible concentrate (DC), Dustable powder (DP), Powder for dry seed treatment (DS), Emulsifiable concentrate (EC), Emulsifiable granule (EG), Emulsion water-in-oil (EO), Emulsifiable powder (EP), Emulsion for seed treatment (ES), Emulsion oil-in-water (EW), Flowable concentrate for seed treatment (FS), Granules (GR), Micro-emulsion (ME), Oil-dispersion (OD), Oil miscible flowable concentrate (OF), Oil miscible liquid (OL), Oil dispersible powder (OP), Suspension concentrate (SC), Suspension concentrate for direct application (SD), Suspo-emulsion (SE), Water soluble granule (SG), Soluble concentrate (SL), Spreading oil (SO), Water soluble powder (SP), Water soluble tablet (ST), Ultra-low volume (ULV) suspension, Tablet (TB), Ultra-low volume (ULV) liquid, Water dispersible granules (WG), Wettable powder (WP), Water dispersible powder for slurry seed treatment (WS), Water dispersible tablet (WT), a mixed formulation of CS and SC (ZC) or a mixed formulation of CS and SE (ZE) and a mixed formulation of CS and EW (ZW). In yet another embodiment of the present invention, the synergistic pesticidal composition is formulated either as Suspension concentrate (SC) or Suspo-emulsion (SE) or Micro-emulsion (ME). In yet another embodiment of the present invention, the synergistic pesticidal composition is formulated either as a Suspension Concentrate (SC) or Emulsifiable concentrate (EC) or a mixed formulation of CS and SC (ZC). In yet another preferred embodiment of the present invention, the pesticidal composition is formulated as Suspension Concentrate (SC). In yet another preferred embodiment, the synergistic pesticidal composition is formulated as Suspo-emulsion (SE). In yet another preferred embodiment, the synergistic pesticidal composition is formulated as micro-emulsion (ME). The composition of the present invention are effective in controlling broad spectrum of pests in various plants/crops including but not limited to paddy, sugarcane, cotton, cabbage, cauliflower, tobacco, groundnut , wheat, onion, rose , grapes, tomatoes, chilli, brinjal, okra (Bhindi), cumin, Mango, potatoes, leafy vegetables, oil crops, soybean, flowering ornamentals vine crops and fruit trees. , soybean, barley, oats, rye, triticale, bananas, sunflower, potatoes, pasture, alfalfa, grasses, turf, sorghum, rapeseed, Brassica spp., sugar beet, citrus, pears, watermelon, egg-plants, lettuce, iceberg lettuce, pepper, cucumber, squash, melon, bean, dry-beans, peas, leek, garlic, carrot, tobacco, coffee, Tea ,apple, Grains Millet, Pulses Dry Fruits,Nuts Spices & Oil Seeds ,turf and forage, cruciferous, cucurbits, grapevines, pepper, fodder beet, oil seed rape, pansy. The composition of the present invention are effective in controlling broad spectrum of pests but not limited to bollworms, Whitefly, aphids, Jassids, thrips, Fruit borer ( Helicoverpa armigera ), Thrips ( Scirtothrips dorsalis), Shoot and fruit borer (Leucinodes orbonalis), Thrips (Thrips tabaci), leaf miner (Liriomyza trifolii), Chilli Yellow Mite, Scarlet mite (Breviapalpus phoenicis), Maruca vitrata, Pink Boll Worm, Whiteflies, Jassids (Amrasca devastans), Diamond back moth (Plutella xylostella) & Tobacco caterpillar (Spodoptera litura), Yellow stem borer (Scirpophaga incertulus), mites, Red spider mites (Tetranychus urticae, oligonychus cofeeaea), Boll worms, Diamond back moth, Pod borer, Gundhibug, Stem fly, Pod fly, Rice Hispa, Green leaf hopper, Gall midge, Semilooper, Tea looper, Fall armyworm, Bollworms complex, Shoot fly, Tea mosquito bug, Stem fly, Leafeating caterpillar, Yellow Mites(Polyphagotarsonemus latus), Fruit borer (Spodoptera litura & Helicoverpa armigera), European Red Mite (Panonychus ulmi),Leaf folder, Shoot and CapsuleBorer, Green semi looper, Girdle beetle & Tobacco caterpillar Leaf folder & Hispa, Leaf worm, stem borer, leaf folder, termite, early & top shoot borer, diamond back moth, Green Semiloopers, Stem fly, Girdle beetle, pod borer and fruit & shoot borer and various Lepidoptera & Coleopteran etc. including insects resistant to chemicals, and shows excellent controlling effects (synergistic controlling effects) which could never be expected from a single component alone. In further embodiment of the present invention, the pesticidal composition of the present invention further comprises one or more agrochemically acceptable excipients including but not limited to the group comprising surfactant/dispersing agent(s)[ionic/anioinic/non-ionic], defoamer or anti- freezing agent(s), emulsifier(s), co-emulsifier(s), safener(s), anti-foaming agent/defoamer(s), wetting agent(s), suspension aid(s), antimicrobial/anti-bacterial agent(s), thickening agent/thickener(s)/rheology modifier, quick coating agent(s) or sticking agents/sticker(s), spreader(s), binder(s), anti-caking agent(s), pH adjusting agent(s), adjuvant(s), filler(s), colorant(s), buffering agent(s), solvent(s), co-solvent(s) or a combination thereof. Additional components may also be included, e.g., protective colloids, adhesives, thixotropic agents, penetration agents, stabilisers, sequestering agents. More generally, the active materials can be combined with any solid or liquid carrier/additive, which complies with usual formulation techniques. Examples of dispersing agent used herein include but not limited to polyaryl phenyl ether phosphate, tristyryl phenol ethylated, acrylic copolymer, ethoxylated tristryl phenol Sulphate, naphthalene sulfonic acid, sodium salt condensate with formaldehyde, ethoxylated oleyl cetyl alcohol, polyalkelene glycol ether, ethoxylated fatty alcohol, sodium polymethyl methacrylate, sodium polyacrylate, sodium lignosulphonate, Sodium naphthalene formaldehyde condensate , calcium lignosulphonate, alkylphenol polyoxyethylene ether, polycarboxylate, fatty alcohol polyoxyethylene ether, alcohol polyglycol ether , Polymethyl methacrylate-polyethylene glycol graft copolymer, Sodium alkyl naphthalene sulfonateformaldehyde condensate, Ethanol 2,2,2 - nitrilotris with alpha-(2,4,6-tris(1-phenylethyl)phenyl)- omega-hydroxypoly(oxy-1,2-ethanediyl) phosphate, Alkyl phenol Polyethanoxy Ether, 2-2- oxydiethanol, Dihydrogen mono oxide or mixtures thereof and present in the range of 2.0-8.0 % by weight of the composition. Examples of wetting agents used herein include but not limited to tristyrylphenol ethoxylate non- ionic emulsifier, mixture of non-ionic surfactants , alkoxylated alcohol, block copolymer, alcohol polyglycol ether, alkyl olefin sulphates, dioctyl sulpho succinate, alkyl naphthalene sulphate, alkyl phenol ethoxylates, tri-styrenated phenol ethoxylate, EO/PO block co-polymers, sodium lauryl sulphate, sodium ligno sulphates Ethanol 2,2,2 -nitrilotris with alpha-(2,4,6-tris(1- phenylethyl)phenyl)- omega-hydroxypoly(oxy-1,2-ethanediyl) phosphate, Polyethylene Polypropylene glycol mono butyl ether Alkyl phenol Polyethanoxy Ether, 2-2- oxydiethanol, Dihydrogen mono oxide or mixtures thereof and present in the range of 2.0-8.0 % by weight of the total composition. Examples of defoamer or antifoaming agent used herein include but not limited to silicon emulsion based anti-foam agents, Siloxane polyalkyleneoxide, Polydimethylsiloxane, Polydimethylsiloxane ,trisiloxane ethoxylates, Glycerol ,silicone oil, silicone compound, C10～C20 saturated fat acid compounds or C8～C10 aliphatic alcohols compound, vegetable oil based antifoam, tallow based fatty acids, polyalkyleneoxide modified polydimethylsiloxane or mixtures thereof and present in the range of 0.5-2.0% % by weight of the total composition. Examples of Anti-freezing agent used herein include but not limited to glycol, propylene glycol, mono ethylene glycol, glycerine, diethylene glycol, sorbitol, urea, mannitol, polyethylene glycol or mixtures thereof and present in the range of 2.0-12 % by weight of the total composition. Examples of anti-bacterial or anti-microbial agents used herein include but not limited to 1,2- benzisothiazolin-3-one, formaldehyde, sodium benzoate/Sodium o-phenylphenate, 5-chloro-2- methyl-4-isothiazolin-3-one & 2- methyl-4-isothiazolin-3-one, potassium sorbate or mixtures thereof and present in the range of 0.1-2.0 % by weight of the composition. Rheology modifiers are sometimes referred to as thickeners or thickening agents, but they do much more than just thicken a formulation. A good rheology modifier structures the formulation but when a force is applied it becomes flowable and easily poured. Rheology modifier or thickeners or thickening agents used herein include but not limited to polysaccharides, carboxymethyl cellulose, bentonite clay, aluminium magnesium silicate, hydroxy propyl cellulose, xanthan gum, gum arabic, gaur gum, carbomer, chitosan, precipitated silica or mixtures thereof and present in the range of 0.1-2.5 % by weight of the composition. Emulsifier is selected from the group consisting of a nonionic surfactant of ethoxylated fatty acid type, such as ethoxylated fatty acid mono-, di- or triglycerides or ethoxylated dialkyl sulfosuccinate, Tri-styrenated phenol ethoxylate , Blend of Calcium Alkyl benzene sulphonate and Tri-styrenated phenol ethxylate an alkylaryl sulfonate, a polyoxyethylene fatty acid ester, a polyoxyethylene sorbitol fatty acid ester, a polyoxyethylene castor oil and a polyoxyethylene hydrogenated castor oil., ethoxylated fatty alcohol type or a block copolymer comprising one or more alkylene oxide block type or mixtures thereof and present in the range of 0.1-25 % by weight of the composition. Solvent is selected from the group comprising heavy aromatic hydrocarbon, N methyl pyrollidone(NMP), Di methyl sulfoxide (DMSO) , N- alcohol, alkyl amide, vegetable oil, mineral oil, aromatic solvents, water or mixtures thereof and present in the range of 0.1-20 % by weight of the composition. An adjuvant used in the present invention is any material that is added to an agrochemical formulation to enhance or modify the performance of the formulation. An adjuvants used in the present invention to make it a safer to ecological environmental, having low toxicity and having no phytotoxicity effects on any part of the plant. In an embodiment, the kit-of-parts comprises an instructions manual, said instructions manual comprising instructions directing a user to admix the components before being used. The process for preparing the present novel synergistic composition can be modified accordingly by any person skilled in the art based on the knowledge of the manufacturing the formulation. However, all such variation and modification is still covered by the scope of present invention. Thus, before describing the present invention in detail, it is to be understood that this invention is not limited to particularly exemplified systems or process parameters that may of course, vary. It is also to be understood that the terminology used herein is for the purpose of describing particular embodiments of the invention only and is not intended to limit the scope of the invention in any manner. The use of examples anywhere in this specification including examples of any terms discussed herein is illustrative only, and in no way limits the scope and meaning of the invention or of any exemplified term. Likewise, the invention is not limited to various embodiments given in this specification. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. In the case of conflict, the present document, including definitions will control. The composition of the present invention can also comprise or may be applied together and/or sequentially with further active compounds. These further compounds can be selected from fertilizers or micronutrient donors or microorganisms or other preparations that influence plant growth, such as inoculants (e.g. a strain of nitrogen-fixing bacteria), and plant inducers. In one embodiment, the composition according to the present invention acts synergistically to control pests in various crops. Through the pesticidal composition of present invention, the number of applications to control wide range of pests appearing at the same time is minimised. The composition of present invention is highly safe to the user and to the environment. The composition offers the user a single homogenous application eliminating the need for tank mix. The composition also is cost-effective as it provides much greater simultaneous control and can be used in a variety of crops with a broader spectrum of protection. Representative example of the present composition of this invention is provided below and is not to be construed as limiting in any manner: Chemical Composition: Compound of Formula I, i.e.3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate (15%) + Hexythiazox 2.2% + Abamectin 1.15% SC No. Ingredients %(w/w) Compound of Formula I, i.e.3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3- 1 0.1-30.0 en-4-yl) 3,3-dimethylbutyrate Technical 2 Hexythiazox Technical 0.1-10.0 3 Abamectin Technical 0.1-10.0 (Dispersing cum wetting agent) Ethanol 2,2,2 -nitrilotris with alpha- (2,4,6-tris(1-phenylethyl)phenyl)- omega-hydroxypoly(oxy-1,2- 4 ethanediyl) phosphate, Alkyl phenol Polyethanoxy Ether, 2.0-8.0 2-2- oxydiethanol, Dihydrogen mono oxide 5 (Anti freezing agent) Propylene Glycol 2.0-12 6 (Defoamer) Polydimethylsiloxane emulsion 0.5-2.0 7 (Anti-bacterial agent) 1,2-Benzisothiazolin-3-one 0.1-2.0 8 (Rheology modifier) Xanthan Gum 0.1-2.5 9 (Filler) DM water QS Total 100 Example 1. Compound of Formula I, i.e.3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate (15% ) + Hexythiazox (2.2%) + Abamectin (1.15%) SC No. Ingredients %(w/w) Compound of Formula I, i.e.3-Mesityl-2- 1 oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- 15.00 dimethylbutyrate Technical 2 Hexythiazox Technical 2.20 3 Abamectin Technical 1.15 Unitop Sc 725 (Dispersing cum wetting 4 3.00 agent) Propylene glycol (PG) [Anti freezing 5 10.00 agent] 6 SAG 1572 (Defoamer) 2.00 7 Proxel Gxl (Anti-bacterial agent) 0.30 8 Agropol 23 W (Rheology modifier) 0.20 9 DM water QS Total 100 Chemical Composition: Compound of Formula I, i.e.3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate (15%) + Hexythiazox (2.2%) + Abamectin (1.15%) SC No. Ingredients %(w/w) Compound of Formula I, i.e.3-Mesityl-2-

1 oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- 15.00 dimethylbutyrate Technical 2 Hexythiazox Technical 2.20 3 Abamectin Technical 1.15 Ethanol 2,2,2 -nitrilotris with alpha- (2,4,6-tris(1-phenylethyl)phenyl)- omega- hydroxypoly(oxy-1,2-ethanediyl) 4 phosphate, Alkyl phenol Polyethanoxy 3.00 Ether, 2-2- oxydiethanol, Dihydrogen mono oxide 5 Propylene Glycol 10.00 6 Polydimethylsiloxane emulsion 2.00 7 1,2-Benzisothiazolin-3-one 0.30 8 Xanthan Gum 0.20 9 DM water QS Total 100 Manufacturing Process: a) The mixture of surfactants (Ethanol 2,2,2 -nitrilotris with alpha-(2,4,6-tris(1- phenylethyl)phenyl)-omega-hydroxypoly(oxy-1,2-ethanediyl) phosphate, Alkyl phenol Polyethanoxy Ether, 2-2- oxydiethanol, Dihydrogen mono oxide) & anti freezing agent (PG) were first diluted in required D.M.Water, and solubilized by high shear mixing then added compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical, Hexythiazox Technical, Abamectin Technical, Polydimethylsiloxane emulsion and mixed to make homogeneous mass. b) The above mixed mass was grinded in Bead Mill. Grinding was carried out until a mean particle size of below 5 microns was obtained. c) Prepared 2% gum solution –100 g DM water was added to 0.3 g of 1,2-Benzisothiazolin- 3-one. To this, it was then added 2 g xanthan gum, under slow stirring. d) After the grinding, 2% water solution of xanthan gum was added under low stirring. e) The mixture was mixed until homogeneous and checked for quality parameter. CHEMICAL COMPOSITION: Sr. No. Ingredients %w/w 1 Compound of Formula I, i.e.3-Mesityl-2- oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical (98% Purity) 15.50 2 Hexythiazox Tech (97% Purity) 2.30 3 Abamectin Tech (82% Purity) 1.23 4 Acrylic Graft Copolymer 3.00 5 Polyalkoxy ethers 2.00 6 Propylene Glycol 10.00 7 Polydimethyl siloxane 2.00 8 1,2-Benzisothiazolin-3-one 0.30 9 Xanthum Gum 0.22 10 D M water Q.S. TOTAL 100.0 FORMULATION PROCESS A. Pre-Mixing 1. Into a clean reactor, D M Water was charged and started stirring. 2. Added Propylene glycol, Acrylic Graft Copolymer, Polyalkoxy ethers, Polydimethyl siloxane and 1,2-Benzisothiazolin-3-one. Mixed well to make a homogeneous solution. 3. To this added Compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en- 4-yl) 3,3-dimethylbutyrate Technical, Hexythiazox technical and Abamectin technical and mixed well to form a homogeneous slurry. B. Wet-Milling process 1. Passed the premix through the bead mill at 400 to 500 Ltr/ hr feed rate so as get a desired particle size. 2. The grinded material was collected in a post blender. C. Post Mixing 1. The milled slurry was collected into the post blender and to this pre hydrated xanthum gum was added. 2. Mixed well till the slurry attained right viscosity. 3. Packed the formulation into HDPE bottles. Example 2. Compound of Formula I, i.e.3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate (20%) + Hexythiazox 3% + Abamectin 0.50% SE Chemical Composition: Sr. Ingredients % (w/w) No. Compound of Formula I, i.e. 3-Mesityl-2-oxo-1- 1 oxaspiro[4.4]non-3-en-4-yl) 3,3-dimethylbutyrate 20.00 Technical 2 _{Hexythiazox Tech.} ^3.00 ³ _{Abamectin Tech.} ^0.50 ⁴ _{Precipitate Silica} ^1.00 ₅ Polymethyl methacrylate-polyethylene glycol graft 3.00 copolymer 6 _{Polyethylene Polypropylene glycol mono butyl ether} ^2.00 7 Sodium alkyl naphthalene sulfonate 0.50 formaldehyde condensate 8 _NMP ^10.00 ⁹ _{Emulsifier –Tri-styrenated phenol ethoxylate} ^10.00 ¹⁰ _{Polydimethylsiloxane} ^0.50 ¹¹ _{1,2-Benzisothiazolin-3-one} ^0.20 ¹² _{Propylene Glycol} ^5.00 ¹³ _{Xanthan Gum} ^0.30 ¹⁴ _{DM water} ^{QS to Make 100} Total 100.00 Manufacturing Process: Preparation of aqueous slurry-The mixture of surfactants (Polymethyl methacrylate-polyethylene glycol graft copolymer, Polyethylene Polypropylene glycol mono butyl ether, sodium salt of naphthalene sulfonate condensate) & anti freezing agent (Propylene Glycol) were first diluted in required D.M.Water, and solubilized by high shear mixing then add Compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3-dimethylbutyrate technical and Hexythiazox technical , deformer, silica and mix to make homogeneous mass. Preparation of organic slurry –NMP was charged into the vessel, then added Abamectin technical into vessel and dissolved it properly. Then added Tri-styrenated phenol ethoxylate. Stirred it for another 30 minutes after the addition is finished. Ensured that solution is clear and homogenized. Addition of organic slurry to aqueous slurry- Slowly started adding organic slurry to aqueous slurry. Made it properly homogenized. The above mixed mass was grinded in Bead Mill. Grinding was carried out until a mean particle size of below 5 microns was obtained. Preparations of 2% gum solution – Took 100 g DM water, then added 0.2 g of Benzisothiazolin- 3-one then slowly-slowly added 3 g xanthan gum while stirring. After the grinding, added 3% water solution of xanthan gum under low stirring. After mixing till homogeneous solution was achieved, checked for quality parameter. Example 3. Compound of Formula I, i.e.3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate (18%) + Hexythiazox 5% + Abamectin 1.25% ME Chemical Composition: Sr. Ingredients % (w/w) No. Compound of Formula I, i.e. 3-Mesityl-2-oxo-1- 1 oxaspiro[4.4]non-3-en-4-yl) 3,3-dimethylbutyrate 18.00 Technical 2 _{Hexythiazox Technical} ^05.00 ³ _{Abamectin Technical} ^1.25 ⁴ _NMP ^20.00 ⁵ _DMSO ^20.00 6 Emulsifier (Blend of Calcium Alkyl benzene 25.00 sulphonate and Tri-styrenated phenol ethxylate) Q.S. to make 7 _{C IX solvent} 100% Total 100.00 Manufacturing Process: As per recipe the required amount of solvent /co-solvent is weighed and transferred to a mixing vessel. The required amount of technicals are weighed and transferred to mixing vessel. After complete homogenization a sample was drawn for selection of emulsifier. Weighed and transferred the emulsifier to mixing vessel. After complete homogenization a sample was drawn for quality check if sample pass the quality check then transferred the materials in storage tank for packaging. In particular, it has now been found, surprisingly that, for example, the pesticidal activity of the composition of present invention, compared with the pesticidal activity of individual components, is synergistic. In fact, various advantageous properties associated with the compositions according to the invention, include but are not limited to: a broadening of the spectrum of pesticidal activity to other pests, for example to resistant strains; a reduction in the rate of application of the active ingredients; adequate control of the pests at a rate of application at which the individual compounds are not very effective, advantageous behaviour during formulating and/or upon application, improved stability, improved crop characteristics including crop yields, more developed root system, increase in plant health including height, improved plant vigor, less plant verse (lodging), and other advantages familiar to a person skilled in the art. Stability Data: Compound of Formula I, i.e.3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical 15%+ Hexythiazox 2.2%+ Abamectin 1.15% SC Sr. Test Parameter Specification Observation Cold test at HST at at 0° ^^ 7days 54° ^^ 14 days No 1 Description The material Compiles Compiles Compiles is in the form of white to off-white colour homogeneous suspension 2 Compound of 15% (±5%) 15.52 15.49 15.38 Formula I, i.e. 3- Mesityl-2-oxo-1- oxaspiro[4.4]non- 3-en-4-yl) 3,3- dimethylbutyrate Technical Content (%w/w) Hexythiazox 2.2% 2.43 2.43 2.46 Content (%w/w) (+10%, -5%) Abamectin 1.15% 1.17 1.17 1.18 Content (%w/w) (+10%, -5%) Suspensibility of Min 80% 99.1 99.2 99.1 Compound of Formula I, i.e. 3- Mesityl-2-oxo-1- oxaspiro[4.4]non- 3-en-4-yl) 3,3- dimethylbutyrate Technical (%w/w) Suspensibility of Min 80% 99.98 99..1 99.5 Hexythiazox (%w/w) Suspensibility of Min 80% 96.73 99.18 99.2 Abamectin (%w/w) Density @27°C 0.99 to 1.10 1.02 1.02 1.02 (g/ml) 9 pH 1.0% 5.0 to 9.0 7.16 7.15 6.53 Aqueous Solution 10 Viscosity @25°C 300 to 700 434 cp 386 cp 408 cp Spindle 63 60 rpm rpm (CPS) 11 Pourability Residue 5.0 3.21 3.34 3.39 (Residue %w/w) max 12 Wet Sieve test 2.0 Max 0.25 0.28 0.55 (Retained on 45 micron)(%w/w) 13 Presistent foam 60 Max 5 5 5 after 1min (ml) Examples on Efficacy: Evaluation of synergistic effect of pesticidal composition of the present invention A synergistic effect exists wherever the action of a combination of active ingredient is greater than the sum of the action of each of the components alone. Therefore, a synergistically effective amount or an effective amount of a synergistic composition or combination is an amount that exhibits greater pesticidal activity than the sum of the pesticidal activities of the individual components. Synergism was calculated by using Colby’s method (Weeds, vol.15 No.1 (Jan 1967), pp.20-2. The synergistic action expected for a given combination of two active components can be calculated as follows: XY E = (X + Y) – --------- 100 The synergistic action expected for a given combination of three active components can be calculated as follows: (XY+YZ+XZ) (XYZ) E = (X + Y + Z) – ----------------- + ------------ 100 10000 Where: E represents expected percentage of pesticidal control for the combination of the two or three active ingredients at defined doses (for example equal to x, y and z, respectively). X is the percentage of pesticidal control observed by the compound (Compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3-dimethylbutyrate Technical) at a defined dose (equal to x). Y is the percentage of pesticidal control observed by the compound (Hexythiazox) at a defined dose (equal to y). Z is the percentage of pesticidal control observed by the compound (Abamectin) at a defined dose (equal to z). When the percentage of pesticidal control observed for the combination is greater than the expected percentage, there is a synergism effect. Observed control (%) Ratio = ----------------------------- Expected control (%) Ratio of O/E > 1, synergism observed The present invention is illustrated by way of examples, the examples are meant for illustrative purposes and should not be construed as limiting. Experiment for Synergistic activity of combination product of present invention: Example 1- Details of experiment on Tea crop: Experiment for synergistic activity of Compound of Formula I, i.e. 3-Mesityl-2-oxo-1- oxaspiro[4.4]non-3-en-4-yl) 3,3-dimethylbutyrate Technical, Hexythiazox and Abamectin for the control of Mites in Chilli crop was conducted under field at Dhuseri Tea Estate, Assam. Experimental layout The field experiment was carried out in randomized block design (RBD) with twenty-nine (29) treatments (28 insecticides and 1 water spray) each replicated thrice. All the plots demarcated by 10 cm high ridges on all sides. Adequate numbers of irrigation channels were also constructed to provide irrigation independently to each plot. Details of the layout Number of treatments : 29 Number of replication : 3 Plot size (m²) : 7.5 m x 6 m Spacing : 75 cm x 75 cm Nozzle used : Hollow cone nozzle and spray shield Sprayer volume used : 1000 L of water /ha The amount of insecticides required to spray was calculated treatment wise and calibrated. Required quantity of all the insecticides from T1 to T28 were mixed in water as per recommendations. Each insecticide solution was added to a hand-held sprayer and sprayed on the plants. Plants treated with water were included as a control. Application of insecticide spray was performed as single directed application. Ten days after each of the insecticide applications, the numbers of live adult and nymph/larva were counted in the treated plants and untreated control plants. For observation three leaves per plant (one from bottom, one from middle and one from top) were marked on 5 tagged plants per replication. Sr. No. Treatment Ratio T1 Compound of Formula I, i.e. 3-Mesityl-2-oxo- 0.1% + 10% + 10% 1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical + Hexythiazox + Abamectin T2 Compound of Formula I, i.e. 3-Mesityl-2-oxo- 15% + 2.2% + 1.15% 1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical + Hexythiazox + Abamectin T3 Compound of Formula I, i.e. 3-Mesityl-2-oxo- 20% + 7% + 0.1% 1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical + Hexythiazox + Abamectin T4 Compound of Formula I, i.e. 3-Mesityl-2-oxo- 30% + 0.1% + 6% 1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical + Hexythiazox + Abamectin T5 Compound of Formula I, i.e. 3-Mesityl-2-oxo- 0.1% + 10% 1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical + Hexythiazox T6 Compound of Formula I, i.e. 3-Mesityl-2-oxo- 15% + 2.2% 1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical + Hexythiazox T7 Compound of Formula I, i.e. 3-Mesityl-2-oxo- 20% + 7% 1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical + Hexythiazox T8 Compound of Formula I, i.e. 3-Mesityl-2-oxo- 30% + 0.1% 1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical + Hexythiazox T9 Compound of Formula I, i.e. 3-Mesityl-2-oxo- 0.1% + 10% 1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical + Abamectin T10 Compound of Formula I, i.e. 3-Mesityl-2-oxo- 15% + 1.15% 1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical + Abamectin T11 Compound of Formula I, i.e. 3-Mesityl-2-oxo- 20% + 0.1% 1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical + Abamectin T12 Compound of Formula I, i.e. 3-Mesityl-2-oxo- 30% + 6% 1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical + Abamectin T13 Hexythiazox + Abamectin 10% + 10% T14 Hexythiazox + Abamectin 2.2% + 1.15% T15 Hexythiazox + Abamectin 7% + 0.1% T16 Hexythiazox + Abamectin 0.1% + 6% T17 Compound of Formula I, i.e. 3-Mesityl-2-oxo- 0.1% 1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical T18 Compound of Formula I, i.e. 3-Mesityl-2-oxo- 15% 1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical T19 Compound of Formula I, i.e. 3-Mesityl-2-oxo- 20% 1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical T20 Compound of Formula I, i.e. 3-Mesityl-2-oxo- 30% 1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical T21 Hexythiazox 10% T22 Hexythiazox 2.2% T23 Hexythiazox 7% T24 Hexythiazox 0.1% T25 Abamectin 10% T26 Abamectin 1.15% T27 Abamectin 0.1% T28 Abamectin 6% T29 Untreated Control (Water Spray) - Transformations of insect data were made where necessary. The data thus obtained were analyzed from Five plants per replication. Post-treatment mite population were recorded at suitable intervals (10 days) after pray from five plants each replication with three leaves each plant (one from bottom, one from middle and one from top) and mean % population reduction is calculated. Corrected % reduction (Observed Control) over control was worked out using below formula. Corrected % reduction = (No. of mites in Treatment) 1 - ________________________________________________x 100 (No. of mites in Control) Based on % reduction against control in solo treatments the Expected control was calculated and compared with Observed control. The results of the trial have been presented here under in Table 1. Table 1: Synergistic effect of combination product of the present invention (Compound of Formula I, i.e.3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3-dimethylbutyrate Technical + Hexythiazox + Abamectin) against Mites population in Tea crop Observed Expected S. Colby Treatment Ratio per cent per cent No. Ratio control control T1 Compound of Formula I, i.e. 3- 0.1% + 10% + Mesityl-2-oxo-1- 10% oxaspiro[4.4]non-3-en-4-yl) 3,3- 71.13 65.05 1.0934 dimethylbutyrate Technical + Hexythiazox + Abamectin T2 Compound of Formula I, i.e. 3- 15% + 2.2% + Mesityl-2-oxo-1- 1.15% oxaspiro[4.4]non-3-en-4-yl) 3,3- 86.35 73.25 1.1788 dimethylbutyrate Technical + Hexythiazox + Abamectin Compound of Formula I, i.e. 3- 20% + 7% + Mesityl-2-oxo-1- 0.1% oxaspiro[4.4]non-3-en-4-yl) 3,3- 76.12 66.07 1.1521 dimethylbutyrate Technical + Hexythiazox + Abamectin Compound of Formula I, i.e. 3- 30% + 0.1% + Mesityl-2-oxo-1- 6% oxaspiro[4.4]non-3-en-4-yl) 3,3- 88.45 78.50 1.1268 dimethylbutyrate Technical + Hexythiazox + Abamectin Compound of Formula I, i.e. 3- 0.1% + 10% Mesityl-2-oxo-1- oxaspiro[4.4]non-3-en-4-yl) 3,3- 24.67 36.29 0.6799 dimethylbutyrate Technical + Hexythiazox Compound of Formula I, i.e. 3- 15% + 2.2% Mesityl-2-oxo-1- oxaspiro[4.4]non-3-en-4-yl) 3,3- 57.22 57.72 0.9914 dimethylbutyrate Technical + Hexythiazox Compound of Formula I, i.e. 3- 20% + 7% Mesityl-2-oxo-1- oxaspiro[4.4]non-3-en-4-yl) 3,3- 50.66 65.53 0.7730 dimethylbutyrate Technical + Hexythiazox Compound of Formula I, i.e. 3- 30% + 0.1% Mesityl-2-oxo-1- oxaspiro[4.4]non-3-en-4-yl) 3,3- 52.76 52.85 0.9982 dimethylbutyrate Technical + Hexythiazox T9 Compound of Formula I, i.e. 3- 0.1% + 10% Mesityl-2-oxo-1- oxaspiro[4.4]non-3-en-4-yl) 3,3- 48.82 49.75 0.9813 dimethylbutyrate Technical + Abamectin T10 Compound of Formula I, i.e. 3- 15% + 1.15% Mesityl-2-oxo-1- oxaspiro[4.4]non-3-en-4-yl) 3,3- 53.81 70.12 0.7674 dimethylbutyrate Technical + Abamectin T11 Compound of Formula I, i.e. 3- 20% + 0.1% Mesityl-2-oxo-1- oxaspiro[4.4]non-3-en-4-yl) 3,3- 53.02 55.24 0.9599 dimethylbutyrate Technical + Abamectin T12 Compound of Formula I, i.e. 3- 30% + 6% Mesityl-2-oxo-1- oxaspiro[4.4]non-3-en-4-yl) 3,3- 69.55 72.82 0.9552 dimethylbutyrate Technical + Abamectin T13 Hexythiazox + Abamectin 10% + 10% 60.89 61.85 0.9846 T14 Hexythiazox + Abamectin 2.2% + 1.15% 42.78 43.39 0.9861 T15 Hexythiazox + Abamectin 7% + 0.1% 21.52 30.86 0.6973 T16 Hexythiazox + Abamectin 0.1% + 6% 36.22 46.80 0.7739 T17 Compound of Formula I, i.e. 3- 0.1% Mesityl-2-oxo-1- 8.40 - - oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical T18 Compound of Formula I, i.e. 3- 15% 52.76 - - Mesityl-2-oxo-1- oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical T19 Compound of Formula I, i.e. 3- 20% Mesityl-2-oxo-1- 50.92 - - oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical T20 Compound of Formula I, i.e. 3- 30% Mesityl-2-oxo-1- 59.58 oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical T21 Hexythiazox 10% 30.45 - - T22 Hexythiazox 2.2% 10.50 - - T23 Hexythiazox 7% 27.03 - - T24 Hexythiazox 0.1% 3.94 - - T25 Abamectin 10% 45.14 - - T26 Abamectin 1.15% 36.75 - - T27 Abamectin 0.1% 5.25 - - T28 Abamectin 6% 44.62 - - T29 Untreated Control (Water Spray) - - - - It is clearly evident from the data shown in above Table 1 for per cent Mites control in Tea crop that the ternary combination composition of the present invention comprising Compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3-dimethylbutyrate Technical, Hexythiazox and Abamectin T1 to T4 showed high synergy. The binary combination of two insecticides Compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical + Hexythiazox (T5 to T8), Compound of Formula I, i.e.3-Mesityl-2- oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3-dimethylbutyrate Technical + Abamectin (T9 to T12) and Hexythiazox + Abamectin (T13 to T16) are compatible but non-synergistic for the control of mites as compared to ternary combination of Compound of Formula I, i.e. 3-Mesityl-2-oxo-1- oxaspiro[4.4]non-3-en-4-yl) 3,3-dimethylbutyrate Technical, Hexythiazox and Abamectin. The binary combinations of two insecticides are thus less effective to control mites as compared to ternary combination of three insecticides Compound of Formula I, i.e. 3-Mesityl-2-oxo-1- oxaspiro[4.4]non-3-en-4-yl) 3,3-dimethylbutyrate Technical + Hexythiazox + Abamectin (T1 to T4). Among four treatments of ternary combination of three insecticides Compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3-dimethylbutyrate Technical + Hexythiazox + Abamectin, T3 with ratio 15% + 2.2% + 1.15% showing highest synergy with optimum control of mites. Experiment for Bio-efficacy evaluation of combination product of present invention: For the ternary combination based on Compound of Formula I, i.e. 3-Mesityl-2-oxo-1- oxaspiro[4.4]non-3-en-4-yl) 3,3-dimethylbutyrate Technical, Hexythiazox and Abamectin evaluation under field conditions, the basic test composition as Compound of Formula I, i.e. 3- Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3-dimethylbutyrate Technical 22.90% SC, Hexythiazox 05.45 % w/w EC and Abamectin 1.9% EC available in the market. For ternary combination, Compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical 15% + Hexythiazox 2.2% + Abamectin 1.15% SC was used. The solo formulations of Compound of Formula I, i.e.3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical 22.90 % SC, Hexythiazox 05.45 % w/w EC and Abamectin 1.9% EC along with binary combination formulation Fenazquin 10% + Bifenthrin 4% EC and solo formulation Propargite 57 % EC available in the market were used for comparison. Example 2: Evaluation of the combination product of the present invention on Tea The synergistic composition of the present invention Compound of Formula I, i.e. 3-Mesityl-2- oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3-dimethylbutyrate Technical 15% + Hexythiazox 2.2% + Abamectin 1.15% SC along with binary combinations and solo formulation were evaluated on Tea crop variety Teen Ali-17. The experiment was conducted at a Khowai Chabagan Shramik Samabaya Samity Ltd., Paharmura, Khowai, Tripura following Randomized Block Design (RBD) with three replications and maintaining a distance of 60 cm x 100 cm between plants and rows. The application of treatments was started with the insect incidence over economic threshold level (ETL) incidence on the crop in experimental plots and repeat application was done after 15 days. The observations were recorded for the following objectives. Objectives: 1. To determine the efficacy of synergistic ternary composition of present invention against Mites in Tea crop and its impact on leaf yield increase. 2. To observe phytotoxicity, if any on Tea. 3. Economics of treatments based on Cost: Benefit Ratio Treatment details: For Bio-efficacy evaluation T1 - Compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical 15% + Hexythiazox 2.2% + Abamectin 1.15% SC @ 500 ml/ha T2 - Compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical 15% + Hexythiazox 2.2% + Abamectin 1.15% SC @ 600 ml/ha T3 - Compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical 15% + Hexythiazox 2.2% + Abamectin 1.15% SC @ 700 ml/ha T4 - Compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical 22.90 % SC @ 400 ml/ha T5 - Hexythiazox 05.45 % w/w EC @ 500 ml/ha T6 - Abamectin 1.9% EC @ 750 ml/ha T7 - Fenazquin 10% + Bifenthrin 4% EC @ 1000 ml/ha T8 - Propargite 57% EC @ 1250 g/ha T9 – Untreated control For phyto-toxicity evaluation T1 - Compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical 15% + Hexythiazox 2.2% + Abamectin 1.15% SC @ 500 ml/ha T2 - Compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical 15% + Hexythiazox 2.2% + Abamectin 1.15% SC @ 600 ml/ha T3 - Compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical 15% + Hexythiazox 2.2% + Abamectin 1.15% SC @ 700 ml/ha T4 - Compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical 15% + Hexythiazox 2.2% + Abamectin 1.15% SC @ 1000 ml/ha T5 - Compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical 15% + Hexythiazox 2.2% + Abamectin 1.15% SC @ 1200 ml/ha T6 - Untreated control Methodology: All the treatments were imposed by high volume knapsack sprayer fitted with hollow cone nozzle on mite incidence over economic threshold level on Tea plants. During the study period, two sprays were given at 15 days interval. Observation on number of mites were taken at seven and fourteen days after the application on ten plants per treatment per replication and mean is presented after each spray. Corrected % reduction (Observed Control) over control was worked out. The crop yield was recorded at each harvest and cumulative dried Tea yield has been expressed as kg/ha. Based on treatment application cost, market price of produce and net profit, the Cost: Benefit Ratio was calculated for the economics of ternary combination treatments. The observations for phyto-toxicity symptoms on Tea crop at 1, 3, 7 and 10 days after each spray were also recorded. The data were subjected to statistically analysis of variance. Results are presented in Tables 2 to 4. Results: The results for mite’s incidence on Tea crop recorded during the experimental period and per cent reduction over untreated control are summarized in Table 2. The data on yield is presented in Table 3. The observations recorded for phytotoxicity symptom is presented in Table 4. Table 2: Field bio-efficacy evaluation of synergistic composition against Mites in Tea crop Mites Per cent Population reduction S. A.I. dose Formulation /Plant Treatment No. (g/ha) dose (ml/ha) After After After After I II I II spray spray spray spray Compound of Formula 75 + 11 + 2.30 1.53 T1 500 69.13 83.27 I, i.e.3-Mesityl-2-oxo- 5.75 (1.67) (1.43) 1-oxaspiro[4.4]non-3- en-4-yl) 3,3- dimethylbutyrate Technical 15% + Hexythiazox 2.2% + Abamectin 1.15% SC Compound of Formula I, i.e.3-Mesityl-2-oxo- 1-oxaspiro[4.4]non-3- en-4-yl) 3,3- 90 + 13.2 + 1.13 0.77 600 84.79 91.64 dimethylbutyrate 6.9 (1.28) (1.13) Technical 15% + Hexythiazox 2.2% + Abamectin 1.15% SC Compound of Formula I, i.e.3-Mesityl-2-oxo- 1-oxaspiro[4.4]non-3- en-4-yl) 3,3- 105 + 15.4 1.05 0.65 700 85.91 92.91 dimethylbutyrate + 8.05 (1.24) (1.07) Technical 15% + Hexythiazox 2.2% + Abamectin 1.15% SC Compound of Formula I, i.e.3-Mesityl-2-oxo- 1-oxaspiro[4.4]non-3- 3.38 2.83 96 400 54.59 69.09 en-4-yl) 3,3- (1.97) (1.83) dimethylbutyrate Technical 22.90 % SC Hexythiazox 05.45 % 5.63 4.67 25 500 24.38 49.09 w/w EC (2.48) (2.27) Abamectin 1.9% EC 14 750 3.87 3.58 48.10 60.91 39 (2.09) (2.02) Fenazquin 10% + 6.00 5.13 T7 100 + 40 1000 19.46 44.00 Bifenthrin 4% EC (2.55) (2.27) 4.32 3.95 T8 Propargite 57% EC 612 1250 42.06 56.91 (2.19) (2.11) 7.45 9.17 T9 Untreated control - - - - (2.82) (3.11) S Em ± - - 0.250 0.414 - - CD (P=0.05) - - 0.757 1.252 - - C.V. - - 11.107 19.989 - - Figures in parentheses are sq root transformed values NS – Non significant Table 3: Field bio-efficacy evaluation of synergistic composition on dried Tea yield and Cost: Benefit Ratio in Tea Formulatio Yield Per cent S. A.I. dose n dose (kg/ha) increase in C:B Treatment No. (g/ ha) (ml/ha) yield over Ratio control Compound of Formula I, i.e.3-Mesityl-2-oxo- 1-oxaspiro[4.4]non-3- en-4-yl) 3,3- 75 + 11 + T1 500 6184 37.48 1:0.557 dimethylbutyrate 5.75 Technical 15% + Hexythiazox 2.2% + Abamectin 1.15% SC Compound of Formula 90 + 13.2 T2 600 6772 50.56 1:0.677 I, i.e.3-Mesityl-2-oxo- + 6.9 1-oxaspiro[4.4]non-3- en-4-yl) 3,3- dimethylbutyrate Technical 15% + Hexythiazox 2.2% + Abamectin 1.15% SC Compound of Formula I, i.e.3-Mesityl-2-oxo- 1-oxaspiro[4.4]non-3- 105 + en-4-yl) 3,3- 15.4 + 700 6812 51.45 1:0.659 dimethylbutyrate 8.05 Technical 15% + Hexythiazox 2.2% + Abamectin 1.15% SC Compound of Formula I, i.e.3-Mesityl-2-oxo- 1-oxaspiro[4.4]non-3- 96 400 5714 27.03 1:0.432 en-4-yl) 3,3- dimethylbutyrate Technical 22.90 % SC Hexythiazox 05.45 % 25 500 4961 10.29 1:0.315 w/w EC Abamectin 1.9% EC 14 750 5430 20.72 1:0.261 Fenazquin 10% + 100 + 40 1000 4781 6.29 1:0.264 Bifenthrin 4% EC Propargite 57% EC 612 1250 5110 13.61 1:0.321 Untreated control - - 4498 - 1:0.236 S Em ± - - 53.015 - - CD (P=0.05) 160.30 - - - - 8 C.V. - - 1.644 - - Table 4: Phytotoxicity evaluation of synergistic composition on Tea plants Formu- Phytotoxicity parameters observed* S. A.I. dose lation (mean data recorded at 1, 3, 7 and 10 days after Treatment No. (g/ ha) dose each spray) (ml/ha) L W N V E H Compound of Formula I, i.e.3-Mesityl-2-oxo- 1-oxaspiro[4.4]non-3- en-4-yl) 3,3- 75 + 11 + T1 500 0 0 0 0 0 0 dimethylbutyrate 5.75 Technical 15% + Hexythiazox 2.2% + Abamectin 1.15% SC Compound of Formula I, i.e.3-Mesityl-2-oxo- 1-oxaspiro[4.4]non-3- en-4-yl) 3,3- 90 + 13.2 T2 600 0 0 0 0 0 0 dimethylbutyrate + 6.9 Technical 15% + Hexythiazox 2.2% + Abamectin 1.15% SC Compound of Formula I, i.e.3-Mesityl-2-oxo- 1-oxaspiro[4.4]non-3- 105 + en-4-yl) 3,3- T3 15.4 + 700 0 0 0 0 0 0 dimethylbutyrate 8.05 Technical 15% + Hexythiazox 2.2% + Abamectin 1.15% SC Compound of Formula I, i.e.3-Mesityl-2-oxo- 1-oxaspiro[4.4]non-3- en-4-yl) 3,3- 150 + 22

T4 1000 0 0 0 0 0 0 dimethylbutyrate + 11.5 Technical 15% + Hexythiazox 2.2% + Abamectin 1.15% SC Compound of Formula I, i.e.3-Mesityl-2-oxo- 1-oxaspiro[4.4]non-3- 180 + en-4-yl) 3,3- T5 26.4 + 1200 0 0 0 0 0 0 dimethylbutyrate 13.8 Technical 15% + Hexythiazox 2.2% + Abamectin 1.15% SC T6 Untreated control -- -- 0 0 0 0 0 0 L – Leaf injury on tips/ surface, W – Wilting, N – Necrosis, V – Vein clearing, E – Epinasty, H – Hyponasty *Based on 0-10 scale where: 0=0%, 1=1-10%, 2=11-20%, 3=21-30%, 4=31-40%, 5=41-50%, 6=51- 60%, 7=61-70%, 8=71-80%, 9=81-90%,10=91-100% From the example, it evident that the synergistic composition of the present invention is better in protecting Tea crop from mites (Table 2) in comparison to registered solo formulations available in market and other market standard products comprising Compound of Formula I, i.e.3-Mesityl- 2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3-dimethylbutyrate Technical 22.90 % SC, Abamectin 1.9% EC, Hexythiazox 05.45 % w/w EC, Fenazquin 10% + Bifenthrin 4% EC and Propargite 57% EC at mentioned dosage levels. The yield of Green tea leaves also improved in the ternary combination of Compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3-dimethylbutyrate Technical 15% + Hexythiazox 2.2% + Abamectin 1.15% SC as compared to binary and solo components (Table 3). Reasons of higher crop yield may be due to better control of target insects. The Cost: Benefit Ratio revealed that ternary combination @ 600 ml/ha was most economical with higher Cost: Benefit Ratio followed by 700 ml/ha and 500 ml/ha dose formulation as compared to other binary and solo treatments (Table 3). The disclosed combination Compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3- en-4-yl) 3,3-dimethylbutyrate Technical 15% + Hexythiazox 2.2% + Abamectin 1.15% SC @ all the doses further showed no phytotoxicity in the Tea crop after 1, 3, 7 and 10 days after each spray (Table 4). It is evident from the above tables of examples that the combination composition of the present invention i.e. Compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate Technical 15% + Hexythiazox 2.2% + Abamectin 1.15% SC resulted in efficient control of mites with higher green leaf yield as compared to the reference standard products (binary compositions and solo products). The Cost: Benefit Ratio also showed that ternary combination Compound of Formula I, i.e.3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3-dimethylbutyrate Technical + Hexythiazox + Abamectin in different formulations types was most economical with higher Cost: Benefit Ratio as compared to other binary and solo products available in market. From the above investigations, it will be observed that numerous modifications and variations can be effectuated without departing from the true spirit and scope of the novel concepts of the present invention. It is to be understood that no limitations with respect to the specific embodiments illustrated is intended or should be inferred. It should be understood that all such modifications and improvements have been deleted herein for the sake of conciseness and readability but are properly within the scope of the following claims. Dated this 27^th day of May, 2024 Dr. Shilpa Arora IN/PA-1238 Patent Agent for the Applicant To The Controller of Patents The Patent Office, At New Delhi

Claims

I CLAIM [CLAIM 1] A synergistic pesticidal composition for pest control of plants, crops comprising: a) compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate or its agrochemically acceptable salts, esters or derivatives; b) Hexythiazox or its agrochemically acceptable salts, esters or derivatives; and c) Abamectin or their agrochemically acceptable salts, esters or derivatives. [CLAIM 2] The pesticidal composition as claimed in claim 1, comprising: a) compound of Formula I, i.e. 3-Mesityl-2-oxo-1-oxaspiro[4.4]non-3-en-4-yl) 3,3- dimethylbutyrate or its agrochemically acceptable salts, esters or derivatives , in the range of 0.1 to 30% by weight of the composition; b) Hexythiazox or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1 to 10% by weight of the composition; and c) Abamectin or its agrochemically acceptable salts, esters or its derivatives, in the range of 0.1 to 10% by weight of the composition. [CLAIM 3] The pesticidal composition as claimed in claim 1 to 2, which further comprises one or more agrochemically acceptable excipients. [CLAIM 4] The pesticidal composition as claimed in claim 3 wherein the agrochemically acceptable excipients comprise of dispersing agent, wetting agent, defoamer or antifoaming agent, rheology modifier, anti-freezing agent, adjuvants, fillers, emulsifying agent, anti-bacterial agents, thickeners/thickening agents, colouring agents, solvents or mixtures thereof. [CLAIM 5] The pesticidal composition as claimed in claim 4, wherein the dispersing agent is selected from the group comprising, polyarylphenyl ether phosphate, tristyryl phenol ethylated, acrylic copolymer, ethoxylated tristryl phenol Sulphate, naphthalene sulfonic acid, sodium salt condensate with formaldehyde, ethoxylated oleyl cetyl alcohol, polyalkelene glycol ether, ethoxylated fatty alcohol, Sodium polymethyl methacrylatnae, sodium polyacrylate, sodium lignosulphonate, calcium lignosulphonate, Sodium naphthalene formaldehyde condensate ,alkylphenol polyoxyethylene ether, polycarboxylate, fatty alcohol polyoxyethylene ether, alcohol polyglycol ether, sodium polymethyl methacrylate, Polymethyl methacrylate-polyethylene glycol graft copolymer, Sodium alkyl naphthalene sulfonate formaldehyde condensate, Ethanol 2,2,2 -nitrilotris with alpha-(2,4,6-tris(1-phenylethyl)phenyl)- omega-hydroxypoly(oxy-1,2-ethanediyl) phosphate, Alkyl phenol Polyethanoxy Ether, 2-2- oxydiethanol, Dihydrogen mono oxide or mixtures thereof and present in the range of 2.0-8.0 % by weight of the composition. [CLAIM 6] The pesticidal composition as claimed in claim 4, wherein the wetting agent is selected form the group comprising tristyrylphenol ethoxylate non-ionic emulsifier, mixture of non-ionic surfactants, alkoxylated alcohol, block copolymer, alcohol polyglycol ether, alkyl olefin sulphates, dioctyl sulpho succinate, alkyl naphthalene sulphate, alkyl phenol ethoxylates, tri- styrenated phenol ethoxylate, EO/PO block co-polymers, sodium lauryl sulphate, sodium ligno sulphates, Ethanol 2,2,2 -nitrilotris with alpha-(2,4,6-tris(1-phenylethyl)phenyl)- omega- hydroxypoly(oxy-1,2-ethanediyl) phosphate, Polyethylene Polypropylene glycol mono butyl ether , Alkyl phenol Polyethanoxy Ether, 2-2- oxydiethanol, Dihydrogen mono oxide or mixtures thereof and present in the range of 2.0-8.0 % by weight of the total composition. [CLAIM 7] The pesticidal composition as claimed in claim 4, wherein the defoamer or antifoaming agent is selected from the group comprising silicon emulsion based anti-foam agents, Siloxane polyalkyleneoxide, Polydimethylsiloxane, trisiloxane ethoxylates, silicone oil, silicone compound, C10～C20 saturated fat acid compounds or C8～C10 aliphatic alcohols compound, vegetable oil based antifoam, tallow based fatty acids, polyalkyleneoxide modified polydimethylsiloxane and mixtures thereof and present in the range of 0.5-2.0% by weight of the composition. [CLAIM 8] The synergistic composition as claimed in claim 4, wherein the anti-freezing agent is selected from the group comprising glycol, propylene glycol, mono ethylene glycol, Glycerol, glycerine, diethylene glycol, sorbitol, urea, mannitol, polyethylene glycol or mixtures thereof and present in the range of 2.0-12 % by weight of the composition. [CLAIM 9] The pesticidal composition as claimed in claim 4, wherein the antimicrobial or anti-bacterial agent is selected from the group comprising 1,2- benzisothiazolin-3-one, formaldehyde, sodium benzoate/Sodium o-phenylphenate, 5-chloro-2-methyl-4-isothiazolin-3-one & 2- methyl-4- isothiazolin-3-one, potassium sorbate or mixtures thereof and present in the range of 0.1-2.0 % by weight of the composition. [CLAIM 10] The pesticidal composition as claimed in claim 4, wherein the thickener/thickening agent is selected from the group comprising polysaccharides, carboxymethyl cellulose, bentonite clay, aluminium magnesium silicate, hydroxy propyl cellulose, xanthan gum, gum arabic, gaur gum, carbomer, chitosan, precipitated silica or mixtures thereof and present in the range of 0.1-2.5 % by weight of the composition. [CLAIM 11] The pesticidal composition as claimed in claim 4, wherein the solvent is selected from the group comprising heavy aromatic hydrocarbon, N-methyl pyrollidone(NMP), Di-methyl sulfoxide(DMSO), N- alcohol, alkyl amide, vegetable oil, mineral oil, aromatic solvents, water or mixtures thereof and present in the range of 0.1-20 % by weight of the composition. [CLAIM 12] The pesticidal composition as claimed in claim 4, wherein the emulsifier is selected from the group consisting of a nonionic surfactant of ethoxylated fatty acid type, such as ethoxylated fatty acid mono-, di- or triglycerides or ethoxylated dialkyl sulfosuccinate, Tri-styrenated phenol ethoxylate ,blend of Calcium Alkyl benzene sulphonate and Tri-styrenated phenol ethxylate an alkylaryl sulfonate, a polyoxyethylene fatty acid ester, a polyoxyethylene sorbitol fatty acid ester, a polyoxyethylene castor oil and a polyoxyethylene hydrogenated castor oil., ethoxylated fatty alcohol type or a block copolymer comprising one or more alkylene oxide block type or mixtures thereof and present in the range of 0.1-25 % by weight of the composition. [CLAIM 13] The pesticidal composition as claimed in claim 1-12, wherein the use of said composition promotes plant health, growth and increases plant, crop yield in the field. [CLAIM 14] The pesticidal composition as claimed in claim 1-13, provide one shot application of novel and effective synergistic pesticidal composition for killing/ controlling highly resistant mites, whitefly and thrips at lower application rates. [CLAIM 15] The pesticidal composition as claimed in any one of the claims 1-14, wherein the composition is in form of suspension concentrate (SC), Suspo-emulsion (SE) or Micro-emulsion (ME) formulation. [CLAIM 16] A kit-of-parts comprising a plurality of components, wherein said plurality of components comprises: a) compound of Formula I i.e. 3-Mesityl-2-oxo-1-oxaspiro [4.4] non-3-en-4-yl) 3,3- dimethylbutyrate or its agrochemically acceptable salts, esters or derivatives; b) Hexythiazox or its agrochemically acceptable salts, esters or derivatives; c) Abamectin or their agrochemically acceptable salts, esters or derivatives. Dated this 27^th day of May, 2024 Dr. Shilpa Arora IN/PA-1238 Patent Agent for the Applicant To The Controller of Patents