WO2024250082A1 - Food supplement containing rosin resin acids - Google Patents

Abstract

The present invention patent relates to a food supplement that comprises a composition of resin acids extracted from the rosin of pines comprising more than 80% (p/p) of resin acids for use in preventing growth of pathogenic microorganisms in the digestive tract of animals, and in modulating the population of microorganisms for intestinal eubiosis, health and well-being of the animal, wherein the invention can be used as a food supplement for all species of livestock and pets. The present invention can be produced in three forms, i.e. basic liquid (pH between 8 and 9), acidic liquid (pH between 5 and 6) and acidic solid. To obtain the basic liquid form, 21% ground rosin, 2% KOH - potassium hydroxide and 77% demineralized water are used and the pine rosin must be ground to 3 mm; these components are mixed in a decantation tank, allowed to rest for a period of 24 hours, and heated until the total volume reduces by 30%. To obtain the acidic form, 50% ground rosin and 50% grain alcohol are used and the pine rosin must be ground to 3 mm; these components are mixed in a decantation tank and allowed to rest for a period of 24 hours. To obtain the solid form, 50% ground rosin and 50% ultrafine calcitic limestone are used and the pine rosin must be ground to 3 mm; the two components are mixed in a "Y"-type mixer.

Description

“FOOD SUPPLEMENT BASED ON ROSIN RESIN ACIDS”.

[001] The present Invention Patent refers to a food supplement comprising a composition of resin acids extracted from pine pitch comprising more than 80% (w/w) of resin acids for use in preventing the growth of pathogenic microorganisms, in fermentation processes in the alcohol and sugar production industry, in the gastrointestinal tract of animals, in the modulation of the population of microorganisms aiming at intestinal eubiosis, animal health and well-being, and can be used as a food supplement for all species of production and companion animals, as well as improving fermentation and yield in the production of sugar and alcohol.

BACKGROUND OF THE INVENTION

[002] The performance of production animals depends on gastrointestinal functions. Gastrointestinal homeostasis is a prerequisite for optimal performance of production animals.

[003] The multifunctional intestine needs to digest feed, absorb nutrients, host a balanced microbiota and maintain the epithelial barrier and immune functions. A balanced intestine supplies the tissues with energy and nutrients, but excludes the invasion and growth of pathogens, thus allowing the good performance of the animals.

[004] The intestinal ecosystem is constantly challenged in commercial animal production. Pathogenic microorganisms, fungal toxins (mycotoxins), antinutritional factors, overcrowding and other stressors disrupt the balance and require energy-consuming epithelial responses such as inflammation. Disturbances in the intestinal environment eventually lead to reduced profitability of animal production. The food industry calls for natural substances to support natural intestinal homeostasis. [005] Just like in animal production, the alcohol and sugar industry in Brazil is looking for substances such as resin acids, which are completely natural, to replace chemical antibiotics, widely used by the industry, but which generate residues, mainly in yeasts that are used in animal nutrition and can no longer contain residues of these antibiotics, to protect end consumers and the environment.

PRODUCTION OF ROSIN AND TURPENTINE FROM PINE RESIN GUM

[006] The vast majority of Brazilian forests where gum resin is extracted still originate from the time of tax incentives in the 1960s and 1970s. These trees were planted in a dense system, aiming only at producing raw material for the pulp and paper industries, making both the extraction of gum resin and the production of logs for the sawmill and laminating industries unfeasible. As they developed, thinning was carried out, leaving them with greater spacing, resulting in greater development of the trunk and crown. This made it possible to extract gum resin, from which turpentine and pitch are produced, which, despite being a secondary activity in the forest, took Brazil from being an importer to an exporter in the 1980s. Among the numerous species that exist in various parts of the world, only those of the genus Pinus are true producers of gum resin, although production varies within this genus, from species to species. The gum resin of Pinus elliottii var. elliottii produces on average 68% pitch, 17% turpentine and 5% solid impurities. Due to its composition, Pinus elliottii var. elliottii produces the best quality gum resin for the pitch and turpentine industries.

PINE TAR

[007] Pitch is obtained through three methods, two of which are destructive (stump pitch and tall oil pitch), and the third is “live” pitch, produced by extracting turpentine from gum, a resin extracted from live trees, the latter being the most widely used method in Brazil. Tree rosin, or dead rosin, is obtained by using the remaining base of the forest cut, together with the thick roots. Tall oil rosin is the result of the mandatory use of the residue (black liquor) from the industrialization of long-fiber cellulose. The extraction of gum resin, through the resin extraction method in live trees, is sustainable and is responsible for 64% of the world's rosin production, and this is called live rosin or colophony. The main application of rosin is for glues used in the manufacture of paper, paints and varnishes, adhesives, synthetic rubbers, with the remainder being applied in the cosmetics, food, pharmaceutical and other areas.

PURPOSE OF THE INVENTION

[008] The aim of the invention is to provide a new type of natural food supplement based on pitch, its composition being based on resin acids from pine pitch with a dual purpose in the intestinal tract of production animals and improving fermentation yield in the production of sugar and alcohol. They protect the epithelial barrier and support the growth of beneficial microbiota and eliminate pathogenic bacteria from the fermentation process of the sugar and alcohol industry.

[009] The effect of resin acids on the small intestinal epithelium of broiler chickens was discovered by the research group of Professors Filip Van Immerseel and Richard Ducatelle from Ghent University. Dietary resin acids reduced the collagen-degrading activity of matrix metalloproteinases (MMPs) in small intestinal tissue and reduced the infiltration of inflammatory T cells into the duodenum. During inflammation, MMPs degrade collagen and other extracellular matrix proteins. This leads to “leaky gut”: decreased barrier functions and loss of epithelial integrity. Dietary resin acids downregulated the activity and expression of a specific MMP associated with inflammation: matrilysin or MMP7. By By suppressing MMP7, resin acids in the diet support epithelial integrity and barrier functions. Reduced infiltration of inflammatory T cells into the duodenum is further evidence of the anti-inflammatory action of resin acids on intestinal homeostasis, inhibiting the loss of epithelial integrity associated with inflammation and favoring the growth of beneficial microorganisms. Gastrointestinal homeostasis is a prerequisite for optimal performance of production animals. The multitasking intestine needs to digest feed, absorb nutrients, host a balanced microbiota, and maintain the epithelial barrier and immune functions. A balanced intestine supplies tissues with energy and nutrients, but excludes the invasion and growth of pathogens, thus allowing good animal performance. The intestinal ecosystem is constantly challenged in commercial animal production. Pathogenic microorganisms, fungal toxins, antinutritional factors, overcrowding, and other stressors disturb the balance and require energy-consuming epithelial responses, such as inflammation. Disturbances in the intestinal environment eventually lead to reduced profitability in livestock farming. In the sugar and alcohol industry, the inclusion of 50 mg per liter of resin acids in the must completely inhibits the bacteria that cause losses in the fermentation process of up to 30%. Industries are asking for tools - natural substances - to support intestinal homeostasis.

DETAILED DESCRIPTION OF THE INVENTION

[0010] The present invention is based on the premise that a food supplement comprising the extraction of resin acids from pitch can be used to prevent pathogenic microorganisms in the digestive tract of animals, naturally increasing their productivity, reducing the use of antimicrobials and also the use of zinc oxide and copper sulfate, which are being banned in several countries due to their accumulation in meat, the environment and causing resistance of microorganisms to antibiotics. Resin acids are present in pine pitch. produced in Brazil, after extracting turpentine from resin collected from conifers.

[0011] It is known that resin acids have antimicrobial and modulatory properties of the gastrointestinal tract, ruminal fermentation and inactivation of mycotoxins present in the raw materials of feed used in Brazil.

[0012] The food supplement of the present invention comprises a composition based on resin acids from pine pitch that comprises more than 80% (w/w) of resin acids. In this context, the term resin acids should be understood as a reference to a composition that can be added to the feed and water to be provided to animals. Resin acids are insoluble in water and are extracted with organic solvents and mixed with vegetable oil (final product in the liquid version) or dried to form a yellowish powder (final product in the solid version). Both final products contain at least 80% resin acids and can be added to the feed and water of domestic animals. In this context, the term food supplement should be understood as a reference to a composition that can be added to water or food and used as such in animal nutrition.

[0013] The levels of resin acids vary, but include, in their greatest quantity, pimaric, communic, palustric, isopimaric, abietic, dihydroabietic and neoabietic acids. In the present invention, the extraction of resin acids from pitch, the composition of the resin acids of the supplement comprises at least one of the resin acids mentioned.

[0014] This supplement can be produced in three forms, namely the basic liquid form (Ph between 8 and 9), the acidic liquid form (Ph between 5 and 6) and the acidic solid form. To obtain the basic liquid form, 21% ground pitch is used; 2% KOH - potassium hydroxide and 77% demineralized water and the pine pitch must be ground to 3 mm; mixed in a settling tank and left to stand for a period of 24 hours. hours and heat until the total volume is reduced by 30%. To obtain the acid form, use 50% ground pitch and 50% cereal alcohol, and grind the pine pitch to 3 mm; mix in a settling tank and let stand for 24 hours. To obtain the solid form, use 50% ground pitch and 50% ultrafine calcitric limestone, and grind the pine pitch to 3 mm; mix the two components in a “Y” type mixer.

[0015] The basic liquid forms (pH between 8 and 9) and acidic forms (pH between 5 and 6) should be added to the animals' drinking water by diluting 1 liter of the product in 1000 liters of water, and can be provided ad libitum to monogastric and ruminant animals. The solid acidic form will be provided via feed, with between 200 and 300 g/t of feed for poultry; between 300 and 400 g/t of feed for pigs; and between 400 and 500 g/t of feed for cattle.

[0016] The basic liquid product will be mixed in the water supply box with acidic pH at a dosage of 1 liter for every thousand liters of water and the water with the product can be supplied normally to the animals via a drinker.

[0017] The acidic liquid product will be mixed in the water supply box of the production sheds where the pH of the water is basic at a dosage of 1 liter for every thousand liters of water and the water with the product can be supplied normally to the animals via a drinker.

[0018] The solid product in powder form will be mixed into the animal feed in the feed factory at a dose of 200 to 1000 g/t of feed and, in the sugar and alcohol industry, at a quantity of 50 mg per liter of must to improve sugar and alcohol productivity, eliminating bacteria that cause losses in the process.

ADDITIONAL CONSIDERATIONS

[0019] The present food supplement comprises a composition based on resin acids reaching more than 80% (w/w) of resin acids for use in preventing the growth of harmful bacteria and/or in preventing gastrointestinal disorders and that the basis of resin acids is pine pitch resulting from the extraction of turpentine. Added to this is the fact that it is the modulation of microorganisms in the animal digestive tract of monogastric and ruminant animals to improve food utilization.

[0020] It should also be noted that, in the present food supplement, the composition based on resin acids comprises at least one of the following resin acids: pimaric, communis, palustric, isopimaric, abietic, dihydroabietic and neoabietic and/or their derivatives. Furthermore, when absorbed into a carrier material or mixed with a carrier, it becomes possible to supply it via the animals' drinking water, and its concentration in the food or water must be between 0.001% and 1% of the dry weight of the total amount of food or water.

CONCLUSION

[0021] In conclusion, it can be stated that the present “FOOD SUPPLEMENT BASED ON ROSIN RESINIC ACIDS”, due to its set of technological innovations, meets all the conditions to apply for the granting of the Invention Patent.

Claims

1 ^a ) “FOOD SUPPLEMENT BASED ON ROSINIC ACIDS” characterized by being able to be produced in three forms, namely the basic liquid form (Ph between 8 and 9), the acidic liquid form (Ph between 5 and 6) and the acidic solid form. 2 ^a ) “FOOD SUPPLEMENT BASED ON ROSINIC ACIDS FROM ROSIN”, according to claim 1, characterized in that, to obtain the basic liquid form, 21% of ground rosin, 2% of KOH - potassium hydroxide and 77% of demineralized water are used and the pine rosin must be ground to 3 mm; mixed in a settling tank and left to stand for a period of 24 hours and heated until a reduction of 30% of the total volume. 3 ^a ) “FOOD SUPPLEMENT BASED ON ROSINIC ACIDS FROM ROSIN”, according to claim 1, characterized in that, to obtain the acid form, 50% ground rosin and 50% cereal alcohol are used and the pine rosin must be ground to 3 mm; mixed in a settling tank and left to stand for a period of 24 hours. 4 ^a ) “FOOD SUPPLEMENT BASED ON ROSINIC ACIDS FROM ROSIN”, according to claim 1, characterized in that, to obtain the solid form, 50% ground rosin and 50% ultrafine calcitric limestone are used and the pine rosin must be ground to 3 mm and the two components must be mixed in a “Y” type mixer.