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WO2024221380A1 - Cassette de tissu et ensemble d'incorporation de tissu - Google Patents

Cassette de tissu et ensemble d'incorporation de tissu Download PDF

Info

Publication number
WO2024221380A1
WO2024221380A1 PCT/CN2023/091456 CN2023091456W WO2024221380A1 WO 2024221380 A1 WO2024221380 A1 WO 2024221380A1 CN 2023091456 W CN2023091456 W CN 2023091456W WO 2024221380 A1 WO2024221380 A1 WO 2024221380A1
Authority
WO
WIPO (PCT)
Prior art keywords
tissue
flexible member
cover
resilient members
cover body
Prior art date
Application number
PCT/CN2023/091456
Other languages
English (en)
Inventor
Bingbing Wang
Xufeng YU
Jinwen SUN
Chun JIN
Original Assignee
Leica Biosystems Nussloch Gmbh
Leica Microsystems Ltd. , Shanghai
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Leica Biosystems Nussloch Gmbh, Leica Microsystems Ltd. , Shanghai filed Critical Leica Biosystems Nussloch Gmbh
Priority to PCT/CN2023/091456 priority Critical patent/WO2024221380A1/fr
Publication of WO2024221380A1 publication Critical patent/WO2024221380A1/fr

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G01N1/312—Apparatus therefor for samples mounted on planar substrates
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/36—Embedding or analogous mounting of samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • G01N1/31—Apparatus therefor
    • G01N2001/315—Basket-type carriers for tissues
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/36—Embedding or analogous mounting of samples
    • G01N2001/366—Moulds; Demoulding

Definitions

  • the present disclosure relates to a field of histological tissue grossing, processing and embedding, and more particularly to a tissue cassette.
  • tissue cassettes are generally used to hold a tissue sample in position during grossing, process and embedding.
  • US 8623300B2 discloses a method of immobilizing one or more biopsy tissues against a support during processing and embedding by using open cell fully reticulated foam material.
  • the foam material may cause tissue sample loss, increase carryover in processing protocol and distract diagnosis analysis.
  • US 2018/0156701 A1 discloses a histological tissue support device which includes a tissue cassette, a frame, a lid and a microtome sectionable resilient structure.
  • the lid is used to compress the resilient structure against a tissue sample.
  • this solution needs a separate resilient structure to retain the tissue sample in place during processing and embedding, which increases difficulty of manufacturing and device cost.
  • Embodiments of the present disclosure seek to solve at least one of the problems existing in the related art to at least some extent.
  • the present disclosure proposes a tissue cassette, which may immobilize multiple tissue samples in the tissue cassette during grossing, processing and embedding.
  • the present disclosure also proposes a tissue embedding assembly including the above-mentioned tissue cassette.
  • the tissue cassette according to embodiments of a first aspect of the present disclosure includes a frame, a cover and a base.
  • the cover is detachably coupled to the frame.
  • the base is detachably coupled to the frame and has an accommodation space configured to contain one or more tissue samples.
  • the cover includes a cover body, a plurality of resilient members and a flexible member.
  • the plurality of resilient members is coupled between the cover body and the flexible member.
  • the flexible member is configured to contact the one or more tissue samples, and the plurality of resilient members is configured to generate a pressure on the one or more tissue samples to retain a position of the one or more tissue samples against the base.
  • the cover includes the plurality of resilient members coupled between the cover body and the flexible member.
  • the flexible member may contact the one or more tissue samples with the same or different heights, and the plurality of resilient members may adjust the pressure on the one or more tissue samples according to the height of the one or more tissue samples to immobilize the one or more tissue samples between the cover and the base.
  • loss or displacement of the one or more tissue samples during grossing, processing and embedding may be avoided without causing artifact on the one or more tissue samples due to flexibility of the flexible member.
  • the flexible member is made of a same material as a remaining part of the cover.
  • the flexible member, the cover body and the plurality of resilient members are made of the same material, and manufacturing of the tissue sample may be simplified.
  • the cover is made entirely of a same material by molding.
  • the cover body, the plurality of resilient members and the flexible member may be formed as an integral structure during single molding.
  • the flexible member is made of a material different from a remaining part of the cover. That is, a material of the flexible member is different from a material of the cover body and a material of the plurality of resilient members.
  • the flexible member may be made of a material with greater flexibility than the cover body and the plurality of resilient members; and the plurality of resilient members may be made of a material with greater resilience than the cover body and the flexible member.
  • the flexible member is fixed to the remaining part of the cover by insert molding or hot welding or gluing.
  • the cover body, the plurality of resilient members and the flexible member made of different materials may be firmly fixed together.
  • the flexible member is made of a two-dimensional porous material such as a mesh, a film or a nonwoven.
  • the two-dimensional porous material may provide sufficient flexibility and help ensure full infiltration of fluids used during processing and embedding procedures.
  • the flexible member is made of a three-dimensional porous material such as foam.
  • the three-dimensional porous material may further provide sufficient flexibility and help ensure full infiltration of fluids used during processing and embedding procedures.
  • the flexible member is a porous rectangular sheet.
  • the shape of the flexible member may facilitate increase of flexibility.
  • each of the resilient members has an L shape.
  • the resilient member may have a simple structure and be easy to fabricate.
  • each of the resilient members comprises a root portion, a horizontal portion and a vertical portion, the root portion is coupled to the cover body, the horizontal portion has a firs end coupled to the root portion and a second end coupled to the vertical portion, the vertical portion has an upper end coupled to the horizontal portion, and a lower end coupled to the flexible member.
  • the plurality of resilient members may be coupled to the flexible member in a uniformly distributed manner, to generate uniform pressure on the one or more tissue sample.
  • each of the resilient members has a snakelike shape.
  • the resilient member may also have other shapes, which is not limited by the present disclosure.
  • the cover body defines a plurality of through holes, and the plurality of resilient members is arranged in the plurality of through holes.
  • the cover body can stably support the resilient members and the flexible member and facilitate infiltration of processing reagents and embedding materials.
  • the cover body comprises an outer ring portion, an inner ring portion and a plurality of snakelike portions coupled between the outer ring portion and the inner ring portion, and the plurality of resilient members is coupled to the inner ring portion.
  • the snakelike portion is resilient and may affect resilience of the inner ring portion and the plurality of resilient members.
  • the cover body has a ring shape, and the plurality of resilient members is coupled between an inner surface of the cover body and a peripheral edge of the flexible member.
  • the cover has a simple structure and easy to fabricate.
  • the tissue embedding assembly includes a tissue cassette according to any one of above-described embodiments; and a mold including a recess configured to receive the base of the tissue cassette.
  • the cover includes the plurality of resilient members coupled between the cover body and the flexible member.
  • the flexible member may contact the one or more tissue samples with the same or different heights, and the plurality of resilient members may adjust the pressure on the one or more tissue samples according to the height of the one or more tissue samples to immobilize the one or more tissue samples between the cover and the base.
  • loss or displacement of the one or more tissue samples during embedding may be avoided without causing artifact on the one or more tissue samples due to flexibility of the flexible member.
  • FIG. 1 is an exploded view of a tissue cassette according to an embodiment of the present disclosure
  • FIG. 2 is a perspective view of a cover according to an embodiment of the present disclosure
  • FIG. 3 is a side view of a cover according to an embodiment of the present disclosure.
  • FIG. 4 is a sectional view taken along line A-A of FIG. 3;
  • FIG. 5 is a perspective view of a tissue cassette according to an embodiment of the present disclosure.
  • FIG. 6 is a top view of a tissue cassette according to an embodiment of the present disclosure.
  • FIG. 7 is a sectional view taken along line B-B of FIG. 6;
  • FIG. 8 is a sectional view taken along line C-C of FIG. 6;
  • FIG. 9 is a sectional view of a tissue cassette according to an embodiment of the present disclosure, in which a tissue sample is held between a cover and a base;
  • FIG. 10 is a perspective view of an embedding assembly according to an embodiment of the present disclosure.
  • FIG. 11 is a sectional view of a tissue embedding assembly according to an embodiment of the present disclosure, in which a tissue cassette is in a first position;
  • FIG. 12 is a sectional view of a tissue embedding assembly according to an embodiment of the present disclosure, in which a tissue cassette is in a second position;
  • FIG. 13 is a perspective view of a tissue embedding assembly according to an embodiment of the present disclosure.
  • FIG. 14 is another perspective view of a tissue embedding assembly according to an embodiment of the present disclosure.
  • FIG. 15 is a perspective view of a tissue embedding assembly according to an embodiment of the present disclosure.
  • FIG. 16 is another perspective view of a tissue embedding assembly according to an embodiment of the present disclosure.
  • FIG. 17 is a perspective view of a tissue embedding assembly according to an embodiment of the present disclosure.
  • FIG. 18 is another perspective view of a tissue embedding assembly according to an embodiment of the present disclosure.
  • FIG. 19 is a perspective view of a tissue embedding assembly according to an embodiment of the present disclosure.
  • FIG. 20 is another perspective view of a tissue embedding assembly according to an embodiment of the present disclosure.
  • relative terms such as “central” , “longitudinal” , “lateral” , “front” , “rear” , “right” , “left” , “inner” , “outer” , “lower” , “upper” , “horizontal” , “vertical” , “above” , “below” , “up” , “top” , “bottom” as well as derivative thereof (e.g., “horizontally” , “downwardly” , “upwardly” , etc. ) should be construed to refer to the orientation as then described or as shown in the drawings under discussion. These relative terms are for convenience of description and do not require that the present disclosure be constructed or operated in a particular orientation.
  • first and second are used herein for purposes of description and are not intended to indicate or imply relative importance or significance or to imply the number of indicated technical features.
  • the feature defined with “first” and “second” may comprise one or more of this feature.
  • “a plurality of” means two or more than two, unless specified otherwise.
  • the terms “mounted, ” “connected, ” “coupled, ” “fixed” and the like are used broadly, and may be, for example, fixed connections, detachable connections, or integral connections; may also be mechanical or electrical connections; may also be direct connections or indirect connections via intervening structures; may also be inner communications or interactions of two elements, which can be understood by those skilled in the art according to specific situations.
  • the orthogonal XYZ-axis is illustrated in order to facilitate the description and determine the directions.
  • the positive direction of the X-axis is the front direction and the negative direction of the X-axis is the rear direction (as an example of a length direction)
  • the positive direction of the Y-axis is the right direction and the negative direction of the Y-axis is the left direction (as an example of a width direction)
  • the positive direction of the Z-axis is the top direction and the negative direction of the Z-axis bottom direction (as an example of a height direction) .
  • a tissue cassette 100, 200, 300, 400, 500 and a tissue embedding assembly 1000 according to embodiments of the present disclosure will be described below with reference to FIGS. 1 to 20.
  • the tissue cassette 100, 200, 300, 400, 500 includes a frame 110, a cover 130, 230, 330, 430, 530 and a base 150.
  • the cover 130, 230, 330, 430, 530 is detachably coupled to the frame 110.
  • the base 150 is detachably coupled to the frame 110 and has an accommodation space configured to contain one or more tissue samples 900.
  • the cover 130, 230, 330, 430, 530 includes a cover body 131, 231, 331, 431, 531, a plurality of resilient members 132, 232, 332, 432, 532 and a flexible member 133, 233, 333, 433, 533.
  • the plurality of resilient members 132, 232, 332, 432, 532 is coupled between the cover body 131, 231, 331, 431, 531 and the flexible member 133, 233, 333, 433, 533.
  • the flexible member 133, 233, 333, 433, 533 is configured to contact the one or more tissue samples 900, and the plurality of resilient members 132, 232, 332, 432, 532 is configured to generate a pressure on the one or more tissue samples 900 to retain a position of the one or more tissue samples 900 against the base 150.
  • the cover 130, 230, 330, 430, 530 includes the plurality of resilient members 132, 232, 332, 432, 532 coupled between the cover body 131, 231, 331, 431, 531 and the flexible member 133, 233, 333, 433, 533.
  • the flexible member 133, 233, 333, 433, 533 may contact the one or more tissue samples 900 with the same or different heights, and the plurality of resilient members 132, 232, 332, 432, 532 may adjust the pressure on the one or more tissue samples 900 according to the height of the one or more tissue samples 900 to immobilize the one or more tissue samples 900 between the cover 130, 230, 330, 430, 530 and the base 150.
  • loss or displacement of the one or more tissue samples 900 during grossing, processing and embedding may be avoided without causing artifact on the one or more tissue samples 900 due to flexibility of the flexible member 133, 233, 333, 433, 533.
  • the flexible member 133, 233, 333, 433, 533 is made of a same material as a remaining part of the cover 130, 230, 330, 430, 530.
  • the flexible member 133, 233, 333, 433, 533, the cover body 131, 231, 331, 431, 531 and the plurality of resilient members 132, 232, 332, 432, 532 are made of the same material, and manufacturing of the tissue cassette 100, 200, 300, 400, 500 may be simplified.
  • the cover 130, 230, 330, 430, 530 is made entirely of a same material by molding.
  • the cover body 131, 231, 331, 431, 531, the plurality of resilient members 132, 232, 332, 432, 532 and the flexible member 133, 233, 333, 433, 533 may be formed as an integral structure during single molding.
  • the flexible member 133, 233, 333, 433, 533 is made of a material different from a remaining part of the cover 130, 230, 330, 430, 530. That is, a material of the flexible member 133, 233, 333, 433, 533 is different from a material of the cover body 131, 231, 331, 431, 531 and a material of the plurality of resilient members 132, 232, 332, 432, 532.
  • the flexible member 133, 233, 333, 433, 533 may be made of a material with greater flexibility than the cover body 131, 231, 331, 431, 531 and the plurality of resilient members 132, 232, 332, 432, 532; and the plurality of resilient members 132, 232, 332, 432, 532 may be made of a material with greater resilience than the cover body 131, 231, 331, 431, 531 and the flexible member 133, 233, 333, 433, 533.
  • the flexible member 133, 233, 333, 433, 533 is fixed to the remaining part of the cover 130, 230, 330, 430, 530 by insert molding or hot welding or gluing.
  • the cover body 131, 231, 331, 431, 531, the plurality of resilient members 132, 232, 332, 432, 532 and the flexible member 133, 233, 333, 433, 533 made of different materials may be firmly fixed together.
  • the flexible member 133, 233, 333, 433, 533 is made of a two-dimensional porous material such as a mesh, a film or a nonwoven.
  • the two-dimensional porous material may provide sufficient flexibility and help ensure full infiltration of fluids used during processing and embedding procedures.
  • the flexible member 133, 233, 333, 433, 533 is made of a three-dimensional porous material such as foam.
  • the three-dimensional porous material may further provide sufficient flexibility and help ensure full infiltration of fluids used during processing and embedding procedures.
  • the flexible member 133, 233, 333, 433, 533 is a porous rectangular sheet.
  • the shape of the flexible member 133, 233, 333, 433, 533 may facilitate increase of flexibility.
  • each of the resilient members 132, 232, 332, 532 has an L shape.
  • the resilient member 132, 232, 332, 532 may have a simple structure and be easy to fabricate.
  • each of the resilient members 132, 232, 332, 532 comprises a root portion 1321, a horizontal portion 1322 and a vertical portion 1323, the root portion 1321 is coupled to the cover body 131, 231, 331, 431, 531, the horizontal portion 1322 has a firs end coupled to the root portion 1321 and a second end coupled to the vertical portion 1323, the vertical portion 1323 has an upper end coupled to the horizontal portion 1322, and a lower end coupled to the flexible member 133, 233, 333, 433, 533.
  • the plurality of resilient members 132, 232, 332, 432, 532 may be coupled to the flexible member 133, 233, 333, 433, 533 in a uniformly distributed manner, to generate uniform pressure on the one or more tissue samples 900.
  • each of the resilient members 432 has a snakelike shape.
  • the resilient member 432 may also have other shapes, which is not limited by the present disclosure.
  • the cover body 131, 231, 531 defines a plurality of through holes 138, 238, 538, and the plurality of resilient members 132, 232, 532 is arranged in the plurality of through holes 138, 238, 538.
  • the cover body 131, 231, 531 can stably support the resilient members 132, 232, 532 and the flexible member 133, 233, 533 and facilitate infiltration of processing reagents and embedding materials.
  • the cover body 331 comprises an outer ring portion 334, an inner ring portion 335 and a plurality of snakelike portions 336 coupled between the outer ring portion 334 and the inner ring portion 335, and the plurality of resilient members 332 is coupled to the inner ring portion 335.
  • the snakelike portion 336 is resilient and may affect resilience of the inner ring portion 335 and the plurality of resilient members 332, 432.
  • the cover body 431 has a ring shape, and the plurality of resilient members 432 is coupled between an inner surface of the cover body 431 and a peripheral edge of the flexible member 433.
  • the cover 430 has a simple structure and easy to fabricate.
  • the tissue embedding assembly 1000 includes a tissue cassette 100, 200, 300, 400, 500 according to any one of above-described embodiments; and a mold 800 including a recess 802 configured to receive the base 150 of the tissue cassette 100, 200, 300, 400, 500.
  • the cover 130, 230, 330, 430, 530 includes the plurality of resilient members 132, 232, 332, 432, 532 coupled between the cover body 131, 231, 331, 431, 531 and the flexible member 133, 233, 333, 433, 533.
  • the flexible member 133, 233, 333, 433, 533 may contact the one or more tissue samples 900 with the same or different heights, and the plurality of resilient members 132, 232, 332, 432, 532 may adjust the pressure on the one or more tissue samples 900 according to the height of the one or more tissue samples 900 to immobilize the one or more tissue samples 900 between the cover 130, 230, 330, 430, 530 and the base 150.
  • loss or displacement of the one or more tissue samples 900 during embedding may be avoided without causing artifact on the one or more tissue samples 900 due to flexibility of the flexible member 133, 233, 333, 433, 533.
  • a tissue cassette 100 for a tissue embedding assembly 1000 will be described in detail below with reference to FIGS. 1 to 9.
  • FIG. 1 is an exploded view of a tissue cassette 100 according to an embodiment of the present disclosure.
  • the tissue cassette 100 includes a frame 110, a cover 130, and a base 150.
  • the cover 130 and the base 150 are detachably coupled to the frame 110.
  • the base 150 is configured to contain one or more tissue samples 900
  • the cover 130 is configured to immobilize the one or more tissue samples 900 against the base 150.
  • the tissue cassette 100 may contain and retain the position of the one or more tissue samples 900 in position during grossing, processing and embedding of the one or more tissue samples 900.
  • the frame 110 has a substantially rectangular shape.
  • the frame 110 includes a first sidewall 111, a second sidewall 112, a third sidewall 113, and a fourth sidewall 114.
  • the first sidewall 111, the second sidewall 112, the third sidewall 113 and the fourth sidewall 114 are coupled end to end in sequence to form a peripheral wall of the frame 110.
  • the first and third sidewalls 111, 113 are arranged oppositely in a front-rear direction (i.e., a length direction of the tissue cassette)
  • the second and fourth sidewalls 112, 114 are arranged oppositely in a left-right direction (i.e., a width direction of the tissue cassette) .
  • a top and a bottom of the frame 110 are open.
  • the first sidewall 111 includes an inclined face 115 on which information about a patient is printed, such as QR (quick response) codes, barcodes, character strings, etc.
  • QR quick response
  • the frame 110 is provided with six first protrusions 116 horizontally protruding inwards from inner sides of the first to fourth sidewalls 111, 112, 113, 114. Two of the first protrusions 116 are distributed on inner sides of the opposite first and third sidewalls 111, 113 of the frame 110, and the other four of the first protrusions 116 are distributed on inner sides of the opposite second and fourth sidewalls 112, 114 of the frame 110.
  • the frame 110 is further provided with eight second protrusions 117 protruding inwards from inner sides of the first to fourth sidewalls 111, 112, 113, 114 and located higher than the first protrusions 116.
  • Four of the eight second protrusions 117 are evenly distributed on the inner sides of the opposite first and third sidewalls 111, 113 of the frame 110, and the other four of the eight second protrusions 117 are evenly distributed on inner sides of the opposite second and fourth sidewalls 112, 114 of the frame 110.
  • the frame 110 is provided with eight third protrusions 118 that protrude inwards from inner sides of the second and fourth sidewalls 112, 114.
  • the third protrusions 118 are lower than the first protrusions 116 and adjacent to the bottom face of the frame 110.
  • the eight third protrusions 118 are evenly distributed on the inner sides of the second and fourth sidewalls 112, 114.
  • the frame 110 is provided with six fourth protrusions 119 that protrude inwards from inner sides of the first to fourth sidewalls 111, 112, 113, 114 of the frame 110.
  • the fourth protrusions 119 are located lower than the first protrusions 116 and slightly higher than the third protrusions 118.
  • Four of the fourth protrusions 119 are evenly distributed on the opposite first and third sidewalls 111, 113, and the other two of the fourth protrusions 119 are evenly distributed on the opposite second and fourth sidewalls 112, 114 of the frame 110.
  • the frame 110 further defines guide grooves in the inner sides of the first to fourth sidewalls 111, 112, 113, 114 extending vertically for guiding movement of the cover 130 within the frame 110.
  • the frame 110 may define eight first guide grooves 120 vertically extending in the inner sides of the first to fourth sidewalls 111, 112, 113, 114.
  • the second protrusions 117 of the frame 110 may be formed in the first guide grooves 120, respectively.
  • the frame 110 may further define eight second guide grooves 121 vertically extending in the inner sides of the second and fourth sidewalls 112, 114.
  • the third protrusions 118 of the frame 110 may be formed in the second guide grooves 121, respectively.
  • the frame 110 may further define six third guide grooves 122 vertically extending in the inner sides of the first through fourth sidewalls 111, 112, 113, 114.
  • the fourth protrusions 119 of the frame 110 may be formed in the third guide grooves 122, respectively.
  • the frame 110 further defines four conical grooves 123 in outer sides of the second and fourth sidewalls 112, 114.
  • the four conical grooves 123 are evenly distributed on the second and fourth sidewalls 112, 114.
  • the conical grooves 123 may facilitate lifting and transferring of the frame 110 or the tissue cassette 100 manually or automatically by using a tool or mechanism that may be engaged in the conical grooves 123.
  • the cover 130 includes a cover body 131, a plurality of resilient members 132 and a flexible member 133.
  • the cover body 131 is detachably coupled to the frame 110.
  • the flexible member 133 is configured to contact one or more tissue samples 900 contained in the base 150.
  • the plurality of resilient members 132 are coupled between the cover body 131 and the flexible member 133, and configured to generate a pressure on the one or more tissue samples 900.
  • the cover 130 may immobilize the one or more tissue samples 900 against the base 150.
  • the plurality of resilient members 132 may adjust the pressure on one or more tissue samples 900 according to a height of the one or more tissue samples 900. That is, the higher the height of the one or more tissue samples 900, the greater the pressure on the one or more tissue samples 900 generated by the plurality of resilient members 132.
  • the cover body 131 is a substantially rectangular plate, and includes a first side 134, a second side 135, a third side 136 and a fourth side 137.
  • the first side 134, the second side 135, the third side 136 and the fourth side 137 are coupled end to end in sequence.
  • the first and third sides 134, 136 are arranged oppositely in a front-rear direction (i.e., a length direction of the tissue cassette)
  • the second and fourth sides 135, 137 are arranged oppositely in a left-right direction (i.e., a width direction of the tissue cassette) .
  • the corners of the cover body 131 are filleted.
  • the cover body 131 is perforated and includes twenty rectangular through holes 138 extending through a top face and a bottom face of the cover body 131 and divided into five rows extending along the front-rear direction.
  • the cover 130 is provided with eight first tabs 139 horizontally protruding outwards from the first to fourth sides 134, 135, 136, 137 and the eight first tabs 139 are evenly distributed on the first to fourth sides 134, 135, 136, 137.
  • the eight first tabs 139 are located close to the bottom face of the cover body 131.
  • the first tabs 139 of the cover body 131 correspond in position to the second protrusions 117 of the frame 110.
  • the first tabs 139 of the cover body 131 may be elastically engaged with the four second protrusions 117 of the frame 110 from below, to limit upward movement of the cover body 131.
  • the first tabs 139 of the cover body 131 correspond in position to the first guide grooves 120 of the frame 110 and configured to be received in the first guide grooves 120 of the frame 110, respectively.
  • the first tabs 139 may be moved along the first guide grooves 120.
  • the cover body 131 is also provided with eight second tabs 140 horizontally protruding outwards from the second and fourth sides 135, 137.
  • the second tabs 140 are evenly distributed on the second and fourth sides 135, 137.
  • the second tabs 140 are located adjacent to the top face of the cover body 131. In other words, the level of the eight second tabs 140 is higher than the level of the eight first tabs 139.
  • the second tabs 140 of the cover body 131 correspond in position to the third protrusions 118 of the frame 110.
  • the second tabs 140 of the cover body 131 can be supported on the third protrusions 118 to limit downward movement of the cover body 131 within the frame 110.
  • the second tabs 140 of the cover body 131 correspond in position to the second guide grooves 121 of the frame 110 and configured to be received in the second guide grooves 121 of the frame 110, respectively.
  • the second tabs 140 of the cover body 131 may be moved along the second guide grooves 121.
  • the cover body 131 is further provided with six third tabs 141 horizontally protruding outwards from the first to fourth sides 134, 135, 136, 137.
  • Four of the third tabs 141 are evenly distributed on the first and third sides 134, 136, and the other two of third tabs 141 are evenly distributed on the second and fourth sides 135, 137.
  • the level of the six third tabs 141 is substantially the same as the level of the eight first tabs 139.
  • the third tabs 141 of the cover body 131 correspond in position to the fourth protrusions 119 of the frame 110.
  • the six third tabs 141 of the cover body 131 may be elastically engaged with the six fourth protrusions 119 of the frame 110 from below, respectively, to limit upward movement of the cover body 131.
  • the third tabs 141 of the cover body 131 correspond in position to the third guide grooves 122 of the frame 110 and configured to be received in the third guide grooves 122 of the frame 110, respectively.
  • the third tabs 141 of the cover body 131 may be moved along the third guide grooves 122.
  • the cover body 131 defines four avoidance notches 142 horizontally recessed from the second and fourth sides 135, 137, and vertically extending through the top face and the bottom face of the cover body 131.
  • the four avoidance notches 142 are evenly distributed on the second and fourth sides 135, 137.
  • the avoidance notches 142 of the cover body 131 correspond in position to the first protrusions 116 of the frame 110, and when the cover body 131 is moved vertically in the frame 110, the cover body 131 can pass through the first protrusions 116 by means of the avoidance notches 142 without hindrance.
  • the cover 130 includes twenty resilient members 132.
  • the twenty resilient members 132 are arranged in the twenty rectangular through holes 138 of the cover body 131, respectively.
  • Front ten resilient members 132 extend forwards from rear surfaces of front ten rectangular through holes 138, respectively.
  • Rear ten resilient members 132 extend rearwards from front surfaces of rear ten rectangular through holes 138, respectively.
  • the base 150 has a substantially rectangular box shape with an open top, and includes a first side plate 151, a second side plate 152, a third side plate 153, a fourth side plate 154 and a bottom plate 155.
  • the first to fourth side plates 151, 152, 153, 154 are coupled end to end in sequence to form a peripheral plate of the base 150, the first and third side plates 151, 153 are arranged oppositely in the front-rear direction, and the second and fourth side plates 152, 154 are arranged oppositely in the left-right direction.
  • the bottom plate 155 is coupled to bottom edges of the first to fourth side plates 151, 152, 153, 154.
  • the bottom plate 155 is perforated, and provided with a plurality of oblong through holes 156. Forty two oblong through holes 156 are arranged in the bottom plate 155 and divided into three rows extending in the front-rear direction.
  • the four side plates of the base 150 extend obliquely and outwardly from bottom to top. That is, the base 150 is tapered from top to bottom. Thus, the solidified paraffin block may be removed from the base 150 easily.
  • the base 150 is provided with a rim 157 horizontally protruding outwards from and surrounding the first through fourth side plates 151, 152 153, 154 adjacent to the top face of the base 150.
  • the base 150 also defines four elongated grooves 158 horizontally extending along outer sides of the first to fourth side plates 151, 152 153, 154, respectively.
  • the base 150 further defines six depressions 159 in a bottom face of the rim 157. Two of the depressions 159 are evenly distributed in the portions of the rim 157 on the first and third side plates 151, 153, and the other four of the depressions 159 are evenly distributed in the portions of the rim 157 on the second and fourth side plates 152, 154.
  • the depressions 159 of the base 150 also correspond in position to the first protrusions 116 of the frame 110, and when the base 150 is mounted in the frame 110, the first protrusions 116 fit in the depressions 159, respectively, to stably support the base 150 in the frame 110.
  • FIG. 2 is a perspective view of a cover 130 according to an embodiment of the present disclosure.
  • each of the twenty resilient members 132 has a substantially L shape and includes a root portion 1321, a horizontal portion 1322 and a vertical portion 1323.
  • the root portion 1321 is coupled to the cover body 131.
  • the horizontal portion 1322 has a firs end coupled to the root portion 1321 and a second end coupled to the vertical portion 1323.
  • the vertical portion 1323 has an upper end coupled to the horizontal portion 1322, and a lower end coupled to the flexible member 133.
  • a width of the horizontal portion 1322 and a width of the vertical portion 1323 are identical.
  • a width of the root portion 1321 is greater than the width of the horizontal portion 1322 or the vertical portion 1323, and the root portion 1321 is tapered from the cover body 131 to the horizontal portion 1322. Furthermore, the root portion 1321 obliquely extends downwards from the cover body 131 to the horizontal portion 1322.
  • the width and length of the L-shaped resilient member 132 are designed and sized to immobilize the one or more tissue samples 900 without creating artifact on the one or more tissue samples 900.
  • the flexible member 133 has a substantially rectangular sheet shape.
  • the flexible member 133 is patterned and includes five pattern units extending in the left-right direction.
  • Each of the pattern units has three rectangular portions 1331 and two first oblong ring portions 1332, the three rectangular portions 1331 and the two first oblong ring portions 1332 are arranged alternately, and each of the two first oblong ring portions 1332 is located between and coupled to two adjacent rectangular portions 1331.
  • the three rectangular portions 1331 in one pattern unit are coupled to the three rectangular portions 1331 in an adjacent pattern unit via three second oblong ring portions 1333, respectively.
  • the rectangular portions 1331, the first oblong ring portions 1332 and the second oblong ring portions 1333 may be designed and sized to ensure uniform pressure distribution.
  • the flexible member 133 may have appropriate flexibility to adapt for multiple tissue samples 900 with different heights, and facilitate infiltration of processing reagents and embedding materials.
  • the flexible member 133 is provided with a plurality of bumps 1334 on a bottom face of the flexible member 133.
  • the plurality of bumps 1334 is configured to contact the one or more tissue samples 900 to prevent horizontal displacement of the one or more tissue samples 900.
  • the plurality of bumps 1334 may also be designed and sized to ensure uniform pressure distribution.
  • FIG. 3 is a side view of a cover 130 according to an embodiment of the present disclosure.
  • FIG. 4 is a sectional view taken along line A-A of FIG. 3.
  • the twenty resilient members 132 are evenly distributed and coupled to the flexible member 133. Specifically, ten of the resilient members 132 are coupled to the left two pattern units, and the other ten of the resilient members 132 are coupled to the right two patter units. Thus, the arrangement of the twenty resilient members 132 may facilitate uniform pressure distribution.
  • FIG. 5 is a perspective view of a tissue cassette 100 according to an embodiment of the present disclosure.
  • FIG. 6 is a top view of a tissue cassette 100 according to an embodiment of the present disclosure.
  • FIG. 7 is a sectional view taken along line B-B of FIG. 6.
  • FIG. 8 is a sectional view taken along line C-C of FIG. 6.
  • the cover 130 and the base 150 are coupled to the frame 110.
  • the cover 130 is supported on the base 150, the first protrusions 116 of the frame 110 fit in the depressions 159 of base 150, and bottom faces of the second protrusions 117 of the frame 110 engage with the first tabs 139 of the cover 130.
  • the cover 130 and the base 150 are stably clamped between the first protrusions 116 and the second protrusions 117 of the frame 110, ensuring reliability of connection.
  • FIG. 9 is a sectional view of a tissue cassette 100 according to an embodiment of the present disclosure, in which a tissue sample 900 is held between a cover 130 and a base 150. As illustrated in FIG. 9, a tissue sample 900 is received in the base 150, and retained against the base 150 by deformation of the resilient members 132 and the flexible member 133 of the cover 130.
  • the tissue cassette 100 may have two positions. In a first position, the cover 130 and the base 150 are mounted in the frame 110, and the bottom face of the base 150 is substantially in the same level as the bottom face of the frame 110. That is, the cover 130 and the base 150 are located at an upper position relative to the frame 110. In a second position, the cover 130 is still mounted in the frame 110, the bottom face of the cover body 131 is substantially in the same level as the bottom face of the frame 110, and the base 150 is separated from the frame 110 and the cover 130 and located outside the frame 110 completely. That is, the cover 130 and the base 150 are located at a lower position relative to the frame 110, as illustrated in FIGS. 11 and 12.
  • the tissue cassette 100 can be converted from the first position to the second position by staging down the cover 130 and the base 150 via a tool or mechanism. After a tissue sample 900 is placed into the tissue cassette 100, the tissue cassette 100 may be kept in the first position until embedding of the tissue sample 900.
  • a tissue embedding assembly 1000 according to embodiments of the present disclosure will be described in detail below with reference to FIGS. 10-12.
  • FIG. 10 is a perspective view of a tissue embedding assembly 1000 according to an embodiment of the present disclosure.
  • FIG. 11 is a sectional view of a tissue embedding assembly 1000 according to an embodiment of the present disclosure, in which the tissue cassette 100 is in the first position.
  • FIG. 12 is a sectional view of a tissue embedding assembly 1000 according to an embodiment of the present disclosure, in which the tissue cassette 100 is in the second position.
  • the tissue embedding assembly 1000 includes a tissue cassette 100 according to any of the above described embodiments and a mold 800.
  • the mold 800 includes a mold body 801, a recess 802 recessed from a top face of the mold body 801, and a step face 803 formed surrounding an opening of the recess 802.
  • the recess 802 is configured to receive the base 150.
  • the step face 803 is configured to fit with the bottom face of the frame 110.
  • a plurality of elongated projections 804 are formed on a bottom wall of the recess 802, and four elongated grooves 805 are defined in four side walls of the recess 802, respectively.
  • the four elongated grooves 805 extend horizontally.
  • the plurality of elongated projections 804 correspond to the plurality of oblong through holes 156 of the base 150 in position and are configured to fit with the oblong through holes 156 of the base 150.
  • the elongated grooves 805 of the mold 800 correspond to the elongated grooves 158 of the base 150 in position and are configured to cooperate with the elongated grooves 158 of the base 150 to define four paraffin accommodating spaces.
  • tissue cassette 100 is mounted in the recess 802 of the mold 800, and the bottom face of the frame 110 fits with the step face 803.
  • the cover 130 and the base 150 are staged down via a tool or mechanism.
  • the cover 130 is in the lower position relative to the frame 110.
  • the base 150 is completely out of the frame 110 and separated from the frame 110 and the cover 130, and the base 150 is just fitted in the recess 802 of the mold 800.
  • the oblong through holes 156 in the bottom wall of the base 150 are fitted with the elongated projections 804 formed on the bottom wall of the recess 802.
  • the elongated grooves 158 in outer sides of the side walls of the base 150 and the elongated grooves 805 in the inner side walls of the recess 802 cooperate to define the four paraffin accommodating spaces.
  • the bottom face of the cover 130 may abut against the top face of the base 150 received in the recess 802. During this process, the multiple tissue samples 900 are maintained stably between the cover 130 and the base 150, without displacement, flipping, overlapping, etc.
  • Molten paraffin may be is dispensed into the mold 800 through the tissue cassette 100 from above, e.g., via through holes in the cover 130, until a level of the molten paraffin reaches a certain position of the frame 110, e.g., a position higher than the top face of the cover 130.
  • the mold 800 is then cooled to solidify the paraffin to obtain a solidified paraffin block.
  • the paraffin block with the frame 110 and the cover 130 may be detached from the mold 800 by a gripper.
  • Four latch claws of the gripper may fit in truncated conical grooves 123 in the frame 110. Then the gripper may move up to lift the frame 110 with the cover 130 and the solidified paraffin block, achieving separation of the paraffin block from the mold 800, with the base 150 retained in the mold 800.
  • the mold 800 may be heated, and the paraffin in the mold 800 is melted gradually.
  • the base 150 is detached form the mold 800.
  • the embedding of the multiple tissue samples 900 is completed.
  • FIGS. 13 and 14 are perspective views of a tissue cassette 200 according to another embodiment of the present disclosure.
  • the tissue cassette 200 has similar structure to the tissue cassette 100 except for a cover 230. Only a structure of the cover 230 is described in detail below, and remaining structures of the tissue cassette 200 may refer to the description of the tissue cassette 100.
  • the cover 230 includes a cover body 231, a plurality of resilient members 232 and a flexible member 233.
  • the flexible member 233 is configured to contact one or more tissue samples 900 contained in the base 150.
  • the plurality of resilient members 232 are coupled between the cover body 231 and the flexible member 233, and configured to generate a pressure on the one or more tissue samples 900.
  • the cover 230 may immobilize the one or more tissue samples 900 against the base 150.
  • the plurality of resilient members 232 may adjust the pressure on one or more tissue samples 900 according to a height of the one or more tissue samples 900. That is, the higher the height of the one or more tissue samples 900, the greater the pressure on the one or more tissue samples 900 generated by the plurality of resilient members 232.
  • the cover body 231 is a substantially rectangular plate.
  • the cover body 231 is perforated and includes twelve rectangular through holes 238 extending through a top face and a bottom face of the cover body and divided into three rows extending along the front-rear direction.
  • a size of the rectangular through holes 238 in a left row or in a right row is greater than a size of the rectangular through holes 238 in a middle row.
  • the cover 230 includes twelve resilient members 232.
  • the twelve resilient members 232 are arranged in the twelve rectangular through holes 238 of the cover body, respectively.
  • the four resilient members 232 in the middle row extend in the front-rear direction. Front two of the four resilient members 232 in the middle row extend forwards from rear surfaces of the rectangular through holes 238 in the middle row; and rear two of the four resilient members 232 in the middle row extend rearwards from front surfaces of the rectangular through holes 238 in the middle row.
  • the four resilient members 232 in the left row or in the right row extend diagonally relative the front-rear direction. Front two of the four resilient members 232 in the left row extend from rear right corners towards front left corners of the rectangular through holes 238 in the left row, and rear two of the four resilient members 232 in the left row extend from front right corners towards rear left corners of the rectangular through holes 238 in the left row. Front two of the four resilient members 232 in the right row extend from rear left corners towards front right corners of the rectangular through holes 238 in the right row, and rear two of the four resilient members 232 in the right row extend from front left corners towards rear right corners of the rectangular through holes 238 in the right row.
  • Each of the twelve resilient members 232 has a structure similar to the resilient members 132 of cover 130, and detailed structure of the twelve resilient members 232 may refer to the description of the resilient members 132 of cover 130.
  • the flexible member 233 has a substantially rectangular sheet shape.
  • the flexible member 233 is patterned and includes five first pattern units 2331 extending in the front-rear direction and four second pattern units 2332 extending in the front-rear direction.
  • Each of first pattern units 2331 has a plurality of hexagonal or pentagonal through holes elongated in the front-rear direction; and each of the second pattern units 2332 has a plurality of hexagonal or pentagonal through holes elongated in the left-right direction.
  • the five first pattern units 2331 and the four second pattern units 2332 are arranged alternately in the left-right direction.
  • the flexible member 133 is provided with a plurality of bumps 1334 on a bottom face of the flexible member 133 and around a peripheral edge of the bottom face of the flexible member 133.
  • the plurality of bumps 1334 is configured to contact the one or more tissue samples 900 to prevent horizontal displacement of the one or more tissue samples 900.
  • FIGS. 15 and 16 are perspective views of a tissue cassette 300 according to another embodiment of the present disclosure.
  • the tissue cassette 300 has similar structure to the tissue cassette 100 except for a cover 330. Only a structure of the cover 330 is described in detail below, and remaining structures of the tissue cassette 300 may refer to the description of the tissue cassette 100.
  • the cover 330 includes a cover body 331, a plurality of resilient members 332 and a flexible member 333.
  • the flexible member 333 is configured to contact one or more tissue samples 900 contained in the base 150.
  • the plurality of resilient members 332 are coupled between the cover body 331 and the flexible member 333, and configured to generate a pressure on the one or more tissue samples 900.
  • the cover 330 may immobilize the one or more tissue samples 900 against the base 150.
  • the plurality of resilient members 332 may adjust the pressure on one or more tissue samples 900 according to a height of the one or more tissue samples 900. That is, the higher the height of the one or more tissue samples 900, the greater the pressure on the one or more tissue samples 900 generated by the plurality of resilient members 332.
  • the cover body 331 is a substantially rectangular shape.
  • the cover body 331 includes an outer ring portion 334, an inner ring portion 335 and a plurality of snakelike portions 336 coupled between the outer ring portion 334 and the inner ring portion 335.
  • the cover 330 includes nine resilient members 332.
  • the nine resilient members 332 are coupled to the inner ring portion 335.
  • the nine resilient members 332 divide into three rows along the left-right direction.
  • the resilient members 332 in a front row extend forwards from a front outer surface of the inner ring portion 335.
  • the resilient members 332 in a rear row extend rearwards from a rear outer surface of the inner ring portion 335.
  • the resilient members 332 in a middle row extend rearwards from a rear surface of a cross beam 337 coupled to an inner surface of the inner ring portion 335.
  • the cover body 331 of the cover 330 in this embodiment is resilient, and the number of the resilient members 332 may be reduced while ensuring providing sufficient pressure on the one or more tissue samples 900.
  • Each of the nine resilient members 332 has a structure similar to the resilient members 132 of cover 130, and detailed structure of the nine resilient members 332 may refer to the description of the resilient members 132 of cover 130.
  • the flexible member 333 has a substantially rectangular sheet shape.
  • the flexible member 333 has a structure similar to the flexible member 133 of the cover 130, and detailed structure of the flexible member 333 may refer to description of the flexible member 133 of the cover 130.
  • FIGS. 17 and 18 are perspective views of a tissue cassette 400 according to another embodiment of the present disclosure.
  • the tissue cassette 400 has similar structure to the tissue cassette 100 except for a cover 430. Only a structure of the cover 430 is described in detail below, and remaining structures of the tissue cassette 400 may refer to the description of the tissue cassette 100.
  • the cover 430 includes a cover body 431, a plurality of resilient members 432 and a flexible member 433.
  • the flexible member 433 is configured to contact one or more tissue samples 900 contained in the base 150.
  • the plurality of resilient members 432 are coupled between the cover body 431 and the flexible member 433, and configured to generate a pressure on the one or more tissue samples 900.
  • the cover 430 may immobilize the one or more tissue samples 900 against the base 150.
  • the plurality of resilient members 432 may adjust the pressure on one or more tissue samples 900 according to a height of the one or more tissue samples 900. That is, the higher the height of the one or more tissue samples 900, the greater the pressure on the one or more tissue samples 900 generated by the plurality of resilient members 432.
  • the cover body 431 is a substantially ring shape, and a top and a bottom of the cover body 431 are open.
  • the cover 430 includes four resilient members 432.
  • Each of the four resilient members 432 has a snakelike shape. Front two of the four resilient members 432 are coupled between a front inner surface of the cover body 431 and a front end of the flexible member 433, and rear two of the four resilient members 432 are coupled between a rear inner surface of the cover body 431 and a rear end of the flexible member 433.
  • the flexible member 433 has a substantially rectangular sheet shape.
  • the flexible member 433 is patterned and includes five pattern units extending in the left-right direction.
  • the patter unit at the front end of the flexible member 433 includes three rectangular ring portions 4331 and two first oblong ring portions 4332, the three rectangular ring portions 4331 and the two first oblong ring portions 4332 are arranged alternately, and each of the two first oblong ring portions 4332 is located between and coupled to two adjacent rectangular ring portions 4331.
  • the patter unit at the rear end of the flexible member 433 includes three second oblong ring portions 4333 and a common bar 4334, and the three second oblong ring portions 4333 are coupled to the common bar 4334.
  • Each of the three middle pattern units has three 8-shaped portions 4335 and two first oblong ring portions 4332, the three 8-shaped portions 4335 and the two first oblong ring portions 4332 are arranged alternately, and each of the two first oblong ring portions 432 is located between and coupled to two adjacent 8-shaped portions 4335.
  • the three 8-shaped portions 4335 in one pattern unit are coupled to the 8-shaped portions 4335 in an adjacent pattern unit via three second oblong ring portions 4333, respectively.
  • the three rectangular ring portions 4331 of the pattern unit at the front end of the flexible member 433 is coupled to the three 8-shaped portions 4335 of an adjacent pattern unit via three second oblong ring portions 4333, respectively.
  • the three second oblong ring portions 4333 of the pattern unit at the rear end of the flexible member 433 is coupled to the three 8-shaped portions 4335 of an adjacent pattern unit, respectively.
  • the cover 430 in this embodiment has a simple structure and easy to manufacture.
  • FIGS. 19 and 20 are perspective views of a tissue cassette 500 according to another embodiment of the present disclosure.
  • the tissue cassette 500 has similar structure to the tissue cassette 100 except for a cover 530. Only a structure of the cover 530 is described in detail below, and remaining structures of the tissue cassette 500 may refer to the description of the tissue cassette 100.
  • the cover 530 includes a cover body 531, a plurality of resilient members 532, a ring member 534 and a flexible member 533.
  • the flexible member 533 is coupled to the ring member 534 and configured to contact one or more tissue samples 900 contained in the base 150.
  • the plurality of resilient members 532 are coupled between the cover body 531 and the ring member 534, and configured to generate a pressure on the one or more tissue samples 900.
  • the cover 530 may immobilize the one or more tissue samples 900 against the base 150.
  • the plurality of resilient members 532 may adjust the pressure on one or more tissue samples 900 according to a height of the one or more tissue samples 900. That is, the higher the height of the one or more tissue samples 900, the greater the pressure on the one or more tissue samples 900 generated by the plurality of resilient members 532.
  • the cover body 531 is a substantially rectangular plate.
  • the cover body 531 is perforated and includes twenty two rectangular through holes 538 extending through a top face and a bottom face of the cover body.
  • the rectangular through holes 538 divide into five rows along the left-right direction.
  • the rectangular through holes 538 in a first row and a last row are elongated in the left-right direction.
  • the rectangular through holes 538 in a second row and a fourth row are elongated in the front-rear direction.
  • the rectangular through holes 538 in a middle row are significantly small in size than the rectangular through holes 538 in other rows.
  • the cover 530 includes eight resilient members 532.
  • Each of the resilient members 532 has a substantially L shape.
  • Four of the resilient members 532 are arranged in the four rectangular through holes 538 in the first and last rows and extend in the left-right direction; the other four of the resilient members 532 are arranged in the four rectangular through holes 538 in the second and fourth rows which are adjacent to an edge of the cover body 531, and extend in the front-rear direction.
  • the ring member 534 is rectangular and coupled between the eight resilient members 532 and the flexible member 534.
  • the flexible member 533 is a substantially rectangular mesh sheet.
  • the cover 530 in this embodiment may provide sufficient pressure on the one or more tissue samples 900 with less resilient members 532.
  • tissue embedding assembly 100 tissue cassette 110 frame 111 first sidewall 112 second sidewall 113 third sidewall 114 fourth sidewall 115 inclined face 116 first protrusion 117 second protrusion 118 third protrusion 119 fourth protrusion 120 first guide groove 121 second guide groove 122 third guide groove 123 conical groove 130 cover 131 cover body 132 resilient member 1321 root portion 1322 horizontal portion 1323 vertical portion 133 flexible member 1331 rectangular portion 1332 first oblong ring portion 1333 second oblong ring portion 1334 bump 134 first side 135 second side 136 third side 137 fourth side 138 rectangular through hole 139 first tab 140 second tab 141 third tab 142 avoidance notch 150 base 151 first side plate 152 second side plate 153 third side plate 154 fourth side plate 155 bottom plate 156 oblong through hole 157 rim 158 elongated groove 159 depression 200 tissue cassette 230 cover 231 cover body 232 resilient member 233 flexible member 2331 first pattern unit 2332 second patter unit 238 rectangular through

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

La présente invention concerne une cassette de tissu qui comprend un cadre, un couvercle et une base. Le couvercle est couplé de manière amovible au cadre. La base est couplée de manière amovible au cadre et a un espace de réception conçu pour contenir un ou plusieurs échantillons de tissu. Le couvercle comprend un corps de couvercle, une pluralité d'éléments élastiques et un élément flexible. La pluralité d'éléments élastiques sont couplés entre le corps de couvercle et l'élément flexible. L'élément flexible est conçu pour entrer en contact avec le ou les échantillons de tissu, et la pluralité d'éléments élastiques sont conçus pour générer une pression sur le ou les échantillons de tissu pour retenir une position du ou des échantillons de tissu contre la base.
PCT/CN2023/091456 2023-04-28 2023-04-28 Cassette de tissu et ensemble d'incorporation de tissu WO2024221380A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PCT/CN2023/091456 WO2024221380A1 (fr) 2023-04-28 2023-04-28 Cassette de tissu et ensemble d'incorporation de tissu

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2023/091456 WO2024221380A1 (fr) 2023-04-28 2023-04-28 Cassette de tissu et ensemble d'incorporation de tissu

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Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050084425A1 (en) * 2002-09-26 2005-04-21 Biopath Automaction, L.L.C. Cassette for handling and holding tissue samples during processing, embedding and microtome procedures, and methods therefor
CN1898022A (zh) * 2003-10-17 2007-01-17 比欧帕斯自动化公司 在处理、包埋和切片程序中用于操作和保持组织样品的盒子及其方法
CN101583315A (zh) * 2006-12-12 2009-11-18 比欧帕斯自动化公司 具有可切片弹性多孔材料的活检载具
CN102292030A (zh) * 2009-01-22 2011-12-21 比欧帕斯自动化公司 用于定向组织样本的切片机切片活检载具
CN104044795A (zh) * 2013-03-15 2014-09-17 徕卡生物系统努斯洛克有限公司 具有偏置元件的组织盒
CN108351283A (zh) * 2015-08-04 2018-07-31 比欧帕斯自动化公司 可切片盒和具有组织固定可分离盖子的包埋框架，以及制备活检组织样品的方法
CN114728284A (zh) * 2019-11-12 2022-07-08 比欧帕斯自动化公司 可切片盒和有连接器的包埋架及制备活检组织样品的方法
WO2022261939A1 (fr) * 2021-06-18 2022-12-22 Leica Biosystems Nussloch Gmbh Cassette pour maintenir et orienter un échantillon de tissu

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050084425A1 (en) * 2002-09-26 2005-04-21 Biopath Automaction, L.L.C. Cassette for handling and holding tissue samples during processing, embedding and microtome procedures, and methods therefor
CN1898022A (zh) * 2003-10-17 2007-01-17 比欧帕斯自动化公司 在处理、包埋和切片程序中用于操作和保持组织样品的盒子及其方法
CN101583315A (zh) * 2006-12-12 2009-11-18 比欧帕斯自动化公司 具有可切片弹性多孔材料的活检载具
CN102292030A (zh) * 2009-01-22 2011-12-21 比欧帕斯自动化公司 用于定向组织样本的切片机切片活检载具
CN104044795A (zh) * 2013-03-15 2014-09-17 徕卡生物系统努斯洛克有限公司 具有偏置元件的组织盒
CN108351283A (zh) * 2015-08-04 2018-07-31 比欧帕斯自动化公司 可切片盒和具有组织固定可分离盖子的包埋框架，以及制备活检组织样品的方法
CN114728284A (zh) * 2019-11-12 2022-07-08 比欧帕斯自动化公司 可切片盒和有连接器的包埋架及制备活检组织样品的方法
WO2022261939A1 (fr) * 2021-06-18 2022-12-22 Leica Biosystems Nussloch Gmbh Cassette pour maintenir et orienter un échantillon de tissu

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