[go: up one dir, main page]

WO2024148321A1 - Dispositif microfluidique 3d et procédé de séparation de particules par taille à partir d'une suspension - Google Patents

Dispositif microfluidique 3d et procédé de séparation de particules par taille à partir d'une suspension Download PDF

Info

Publication number
WO2024148321A1
WO2024148321A1 PCT/US2024/010576 US2024010576W WO2024148321A1 WO 2024148321 A1 WO2024148321 A1 WO 2024148321A1 US 2024010576 W US2024010576 W US 2024010576W WO 2024148321 A1 WO2024148321 A1 WO 2024148321A1
Authority
WO
WIPO (PCT)
Prior art keywords
outlet
center
section
flow channel
particles
Prior art date
Application number
PCT/US2024/010576
Other languages
English (en)
Inventor
Guigen Zhang
Original Assignee
University Of Kentucky Research Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University Of Kentucky Research Foundation filed Critical University Of Kentucky Research Foundation
Publication of WO2024148321A1 publication Critical patent/WO2024148321A1/fr

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D21/00—Separation of suspended solid particles from liquids by sedimentation
    • B01D21/26—Separation of sediment aided by centrifugal force or centripetal force
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D2221/00—Applications of separation devices
    • B01D2221/10—Separation devices for use in medical, pharmaceutical or laboratory applications, e.g. separating amalgam from dental treatment residues
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0652—Sorting or classification of particles or molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/088—Channel loops

Definitions

  • This document relates to a new and improved three-dimensional (3D) microfluidic device and related method for separating particles by size from a suspension sample.
  • the 3D device and method advantageously provide stable alignment, focusing, fractionation and separation of conventional particles as well as bioparticles (cells and vesicles) with high precision and certainty.
  • inertial-based microfluidics have been researched for years for particle separation and fractionation, most of these inertial-focusing-based microfluidic channels take the design with a horizontal-oriented cross-section (i.e., a cross-section that is greater in width than height) To obtain a high flowrate, these channels sometime are made as wide as 500 pm or more to provide a large cross-section area. This horizontal orientation inadvertently introduces difficulties for inertial focusing to work, because in such wider channels, the focusing positions are difficult to reach, adding uncertainty in particle separation and fractionation applications. Although multiple inlets with differential flowrates have been utilized to address some of the problems, to date wide adaptation of microfluidic technologies has not occurred because these devices require real-time monitoring and tuning using a microscope to function properly.
  • This document relates to 3D microfluidic devices with a vertical orientation (i.e., a cross-section that is greater in height than width), an intermediate spiral section and a plurality of discrete outlets that function together to allow for sorting, fractionation and separation of conventional particles and biological particles, such as cells and vesicles, from a suspension.
  • the device and method also provide an unprecedented tunable gating and bracketing capability without requiring any real-time monitoring and tuning under a microscope. For example, when the device and method are used to isolate circulating tumor cells (CTCs) in blood samples, such bracketing capability will aid the distinction of CTCs from other white blood cells, as well as differentiation between vital and non-vital CTCs
  • a new and improved 3D microfluidic device comprising, consisting of or consisting essentially of a flow channel including (a) an inlet, (b) an inlet section, extending along a first tangent, (c) an intermediate spiral section, (d) an outlet section, extending along a second tangent, and (e) a 3D outlet system including a plurality of outlets .
  • the flow channel may be formed by four walls - a first sidewall, a second sidewall, a top wall and a bottom wall.
  • the outlet system may include four outlets, including an upper outlet bordering the top wall, a lower outlet bordering the bottom wall, and sandwiched in between, a center left outlet bordering the first sidewall, and a center right outlet bordering the second sidewall.
  • the cross-section areas of the upper and lower outlets typically are of the same size, and that of the center left and right outlets are of the same size, with the former a few times larger than the latter.
  • the flow channel may have a width of between about 1 pm and about 250 pm and an aspect ratio of channel cross sectional area (H/W) of from about 1.5 to about 4.5.
  • outlet 24 includes (a) two larger outlets 40 including an upper outlet 42 and a lower outlet 44, (b) a first or center left outlet 46 and (c) a second or center right outlet 48.
  • the upper outlet 42 borders the top wall 50 and the lower outlet 44 borders the bottom wall 52.
  • the method allows one to customize and tune the device 10 for the separation of different size particles through the center left outlet 46, the center right outlet 48, the upper outlet 42 and the lower outlet 44. Further, this is accomplished in a number of ways without requiring any real-time monitoring and tuning under a microscope. Those ways include, but are not necessarily limited to, one or more of the following:

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Fluid Mechanics (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

Un dispositif microfluidique comprend un canal d'écoulement comprenant (a) une entrée, (b) une section d'entrée, s'étendant le long d'une première tangente, (c) une section en spirale intermédiaire, (d) une section de sortie, s'étendant le long d'une seconde tangente, et (e) un système de sortie tridimensionnel comprenant une pluralité de sorties. Le canal d'écoulement comporte une section transversale rectangulaire ayant une hauteur H et une largeur W, où H > W. Le dispositif est utile dans un procédé d'alignement, de focalisation, de fractionnement et de séparation stables de particules classiques ainsi que de bioparticules (cellules et vésicules) à partir d'une suspension.
PCT/US2024/010576 2023-01-06 2024-01-05 Dispositif microfluidique 3d et procédé de séparation de particules par taille à partir d'une suspension WO2024148321A1 (fr)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US202363478720P 2023-01-06 2023-01-06
US202363478727P 2023-01-06 2023-01-06
US63/478,720 2023-01-06
US63/478,727 2023-01-06

Publications (1)

Publication Number Publication Date
WO2024148321A1 true WO2024148321A1 (fr) 2024-07-11

Family

ID=91804410

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2024/010576 WO2024148321A1 (fr) 2023-01-06 2024-01-05 Dispositif microfluidique 3d et procédé de séparation de particules par taille à partir d'une suspension

Country Status (1)

Country Link
WO (1) WO2024148321A1 (fr)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5842787A (en) * 1997-10-09 1998-12-01 Caliper Technologies Corporation Microfluidic systems incorporating varied channel dimensions
US20060087918A1 (en) * 2003-06-11 2006-04-27 Agency For Science, Technology And Research Micromixer apparatus and methods of using same
US20130130226A1 (en) * 2010-03-04 2013-05-23 Chwee Teck Lim Microfluidics Sorter For Cell Detection And Isolation
US20180185846A1 (en) * 2015-07-02 2018-07-05 Nanyang Technological University Leukocyte and microparticles fractionation using microfluidics

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5842787A (en) * 1997-10-09 1998-12-01 Caliper Technologies Corporation Microfluidic systems incorporating varied channel dimensions
US20060087918A1 (en) * 2003-06-11 2006-04-27 Agency For Science, Technology And Research Micromixer apparatus and methods of using same
US20130130226A1 (en) * 2010-03-04 2013-05-23 Chwee Teck Lim Microfluidics Sorter For Cell Detection And Isolation
US20180185846A1 (en) * 2015-07-02 2018-07-05 Nanyang Technological University Leukocyte and microparticles fractionation using microfluidics

Similar Documents

Publication Publication Date Title
US11498071B2 (en) Systems and methods for particle focusing in microchannels
AU2016354119B2 (en) Inertial droplet generation and particle encapsulation
EP0231432B1 (fr) Microfiltre à courant transversal
US9597692B2 (en) Micro-fluidic device for sorting particles, and methods for sorting particles
JP2007503597A (ja) 血液から赤血球および血小板をサイズに基づいて除去するための微少流体システム
EP1787729A1 (fr) appareil classeur de type cyclone, méthode de préparation de toner et toner
JP2009113035A (ja) 流体から中立浮力粒子を分離するシステムおよび方法
DE112011102770T5 (de) Mikrofluidische Einheit mit Hilfs- und Seitenkanälen
WO2017083391A1 (fr) Dispositifs microfluidiques pour tri cellulaire
DE102017204002A1 (de) Zentrifugo-pneumatisches schalten von flüssigkeit
WO2024148321A1 (fr) Dispositif microfluidique 3d et procédé de séparation de particules par taille à partir d'une suspension
AU2021383756A1 (en) Methods and systems for mechanoporation-based payload delivery into biological cells
WO2019057894A1 (fr) Procédé de mise en forme de corps déformables et dispositifs associés
JP2020534142A (ja) マイクロ流体システムにおける粒子選別
KR102263754B1 (ko) 비 뉴턴 유체와 미세 유체 채널을 이용한 바이오 샘플 분류방법 및 이를 이용한 미세 유체 칩
US12036553B2 (en) Method of microfluidic particle separation enhancement and the device thereof
EP1432490B1 (fr) Procede et dispositif de separation de suspensions
DE69518479T2 (de) Gasstrom-Klassierer und Verfahren zur Herstellung von Toner
Bayareh et al. Cancer cell separation using passive mechanisms: A review
DE102013207232A1 (de) Sedimentationsvorrichtung, insbesondere für Partikel, sowie Kartusche
CN111040938A (zh) 微流控芯片及分选方法
RU2782098C1 (ru) Способ улучшения разделения микрожидкостных частиц и устройство для способа
CN119771623A (zh) 基于离心微流控无堵塞多级微塑料颗粒分级分选装置及检测方法
WO2025064694A1 (fr) Appareil et procédé de commande d'écoulement de fluide
WO2024194145A1 (fr) Module fluidique, dispositif de traitement fluidique et procédé impliquant une égalisation de pression temporaire dans une chambre pneumatique

Legal Events

Date Code Title Description
121 Ep: the epo has been informed by wipo that ep was designated in this application

Ref document number: 24739027

Country of ref document: EP

Kind code of ref document: A1