WO2023283969A1 - Nucleic acid detection card box and assembly process therefor - Google Patents

Abstract

A nucleic acid detection card box, comprising a sampling tube (1) and a box body (2). The box body (2) comprises a box main body (22), and an upper cover plate (21) and a lower cover plate (23), which respectively cover an upper portion and a lower portion of the box main body, wherein several cavities containing substances used for nucleic acid extraction are provided in the box main body (22), and the sampling tube (1) is in communication with one of the cavities; and the upper cover plate (21) is provided with a corresponding feeding hole, the lower cover plate (23) and the box main body (22) are bonded to form fluid channels, which respectively communicate with the cavities, and the fluid channels selectively connect to each other. A fully closed-type card box structure with a delicate design is used; the cost is low; operation is extremely simple; extraction, amplification and detection can be integrated; and the structure is suitable for various environments, for example, as large as an emergency department of a tertiary hospital or as small as a community and township health center, and is particularly suitable for environments where conventional instruments are not suitable for working at present.

Description

A nucleic acid detection cartridge and its assembly process technical field

The invention relates to nucleic acid extraction technology, in particular to a nucleic acid detection cartridge and a corresponding assembly process.

Background technique

As a popular direction for the development of molecular diagnostics in the future, molecular diagnostic POCT combines the characteristics of compactness, flexibility, convenience, speed, and accuracy. It has more and wider application scenarios and fields than traditional molecular diagnostics. The reason for the layout of this field; however, to realize the true sense of "sample in, result out" molecular diagnostic POCT needs to have the following basic elements: the design of a fully enclosed cartridge, the integration of extraction + amplification + detection, operation Minimalist, low-cost, multiplex PCR technology, reagent room temperature technology, rapid extraction and purification technology, portable molecular diagnostic POCT instrument. At present, there are no molecular POCT products that truly realize the above basic elements on the market.

Contents of the invention

In order to solve the deficiencies in the existing technology, the present invention provides a nucleic acid detection cartridge and its assembly process, which is an exquisitely designed fully enclosed cartridge structure, low in cost, extremely simple in operation, and can realize the integration of extraction, amplification and detection change.

To achieve the above object, the present invention provides the following technical solutions:

A nucleic acid detection cartridge, comprising a sampling tube and a box body, the box body is provided with several cavities for containing materials used for nucleic acid extraction and several fluid channels communicated with the cavities, and the sampling tube is connected to one of the cavities body, the fluid channels can be selectively communicated with each other.

Further, the box body includes a box body and an upper cover plate covering the top of the box body, the upper cover plate is provided with a feed hole corresponding to the cavity, and the upper surface of the upper cover plate is covered with a top sealing film for sealing.

Preferably, the feeding hole corresponding to the cavity containing the volatile substance is configured with a sealing cork.

Further, the fluid channel is divided into a first-layer fluid channel and a second-layer fluid channel, and the lower end surface of the box body is provided with a plurality of first micro-pipes, and the first micro-pipes communicate with the cavity through holes;

The box body also includes a lower cover plate covering the bottom of the box body, the lower end surface of the lower cover plate is provided with a plurality of second micro-pipes, the lower end surface of the lower cover plate covers the bottom sealing film for sealing, The second micro-pipe cooperates with the bottom sealing film to form a second-layer fluid channel, and the first micro-pipe cooperates with the upper end surface of the lower cover to form a first-layer fluid channel.

Further, an amplification detection area is also set on the lower cover, the amplification detection area includes an amplification detection array and a detection micropipe communicating with the amplification detection array, and the upper end of the amplification detection area covers the detection area. The area sealing film is used for sealing.

Preferably, the second micropipe is provided with several fluid state cavities, and the box body is provided with fluid observation windows corresponding to the fluid state cavities.

Further, the nucleic acid detection cartridge further includes a rotation mechanism, and the rotation mechanism controls the selective communication between the fluid channels through rotation.

Specifically, the rotating mechanism includes a soft cushion and a rotating valve connected in sequence. The lower end surface of the soft cushion is provided with a number of positioning holes, and the positioning holes cooperate with a number of positioning columns arranged on the box body to fix the rotating valve. Mechanism, the cushion is connected to the fluid channel through a number of through holes, the lower end surface of the rotary valve is provided with a liquid flow channel and a gas flow channel, and the liquid flow channel can selectively communicate with the fluid through the through holes The gas channel is selectively connected to the fluid channel through the through hole.

Further, the rotating mechanism also includes a limit ring and a limit wall, the lower end surface of the limit ring is set as a bonding area and a smooth area, and the smooth area is pressed against the edge of the rotary valve for sealing. The bonding area is connected to the limiting wall, and the limiting ring cooperates with the limiting wall to form a rotation space.

Further, the box body is also provided with a gas inlet and outlet channel, the gas inlet and outlet channel can be selectively connected to the gas flow channel or cavity, the lower port of the gas inlet and outlet channel is covered with a waterproof and breathable film, the The gas inlet and outlet channels control the flow direction of substances used in nucleic acid extraction by providing gas power.

Further, the length of the box body is 50mm-70mm, and/or the width is 20mm-40mm, and/or the height is 15mm-30mm.

The present invention also provides a nucleic acid detection cartridge assembly process, comprising the following steps:

Step 1: The lower cover plate is bonded to the box body to form a fluid channel;

Step 2: The box body is bonded with the upper cover to form a complete cavity;

Step 3: Assemble the rotating mechanism, put the soft cushion into the limit wall, fix the rotating mechanism, ensure that the through holes on the soft cushion correspond to the corresponding through holes on the upper end surface of the box body, and then install the rotating valve above the cushion;

Step 4: adding liquid, adding substances used for nucleic acid extraction into the cavity one by one, and then sealing;

Step 5: Add primers to the amplification detection array and seal it.

Further, the assembly process of said step 1 includes:

First, the bottom sealing film is bonded to the lower cover to form a second layer of fluid channels;

Secondly, cover the waterproof and breathable membrane to the position of the gas inlet and outlet channel on the lower end surface of the box body;

Again, the lower cover plate is bonded to the box body to form the first layer of fluid channels.

The invention realizes the molecular diagnosis POCT in the true sense of "sample in, result out". It adopts the design of a fully enclosed cartridge with a compact structure and greatly reduces the cost; all the reagents used are pre-installed in the detection cartridge, no need Added separately; realize the integration of extraction + amplification + detection, and at the same time have low requirements for supporting instruments, and only need simple operation to realize the whole process of molecular diagnosis. The present invention also adopts multiple PCR technology, reagent room temperature technology, rapid extraction and purification technology, and can realize the whole-process extraction and detection of nucleic acid without a professional molecular biology laboratory. Departments, as small as community and township health centers, are especially suitable for environments where conventional instruments are not suitable for work.

Description of drawings

Fig. 1 is a schematic diagram of the overall structure of a nucleic acid detection cartridge of the present invention;

Fig. 2 is an exploded view from the upper perspective of a nucleic acid detection cartridge of the present invention;

Fig. 3 is an exploded view of a nucleic acid detection cartridge of the present invention from a lower perspective;

Fig. 4 is a schematic view of the front structure of the box body of the present invention;

Fig. 5 is a schematic diagram of the reverse structure of the box body of the present invention;

Fig. 6 is the bottom view of the box body of the present invention;

Figure 7 is a top view of the box body of the present invention;

Fig. 8 is a schematic view of the front structure of the upper cover plate of the present invention;

Fig. 9 is a schematic diagram of the reverse structure of the upper cover plate of the present invention;

Fig. 10 is a schematic view of the front structure of the lower cover plate of the present invention;

Fig. 11 is a schematic diagram of the reverse structure of the lower cover plate of the present invention;

Fig. 12 is a schematic view of the front structure of the rotary valve of the present invention;

Fig. 13 is a schematic diagram of the reverse structure of the rotary valve of the present invention;

Fig. 14 is a schematic view of the front structure of the cushion of the present invention;

Fig. 15 is a schematic diagram of the back structure of the cushion of the present invention;

Fig. 16 is a schematic view of the front structure of the limiting ring of the present invention;

Fig. 17 is a schematic diagram of the reverse structure of the limiting ring of the present invention;

Figure 18 is a schematic diagram of the use of the rotary valve in the initial position of the present invention;

Fig. 19 is a schematic diagram of the use of the rotary valve in the second step of the use method of the present invention;

In the figure, 1 sampling tube; 2 box body; 3 rotating mechanism; 21 upper cover plate; 22 box body; 23 lower cover plate; 221 first micro-pipe; 211 feeding hole; 214 Reagent chamber cover; 121 Amplification detection array; 122 Detection micro-pipe; 123 Detection area sealing film; 231 Second micro-pipe; Rotary valve; 33 cushion; 34 limit wall; 311 bonding area; 312 smooth area; 321 long groove; 322 short groove; 331 positioning hole; 332 positioning column; 101 magnetic bead cavity; 102 extraction cavity; Liquid chamber; 104 sample chamber; 105 cleaning one chamber; 106 cleaning two chambers; 107 elution chamber; 108 reagent chamber; 109 spare chamber; 1010 air hole A; 1011 air hole B; 1013 air hole C; .

detailed description

The following specific examples illustrate the implementation of the present invention. Those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. Obviously, the described embodiments are part of the present invention. , but not all examples. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

In order to make it easier for those skilled in the art to understand the present invention, the specific implementation manners of the present invention will be described below with reference to the accompanying drawings.

Referring to Figures 1 to 19, a nucleic acid detection cartridge is disclosed, which includes a sampling tube 1 and a box body 2. Inside the box body 2 are provided several cavities for containing substances used for nucleic acid extraction and several fluid channels communicating with the cavities , the sampling tube 1 is connected to one of the cavities, and several fluid channels can be selectively communicated with each other.

The nucleic acid detection cartridge of the present invention is very delicate in design, flexible in use, and greatly reduces various costs. As shown in Figure 1, the length range (L) of the box body 2 is 50mm-70mm, preferably 70mm; the width range ( W) is 20mm-40mm, preferably 33mm; height range (H) is 15mm-30mm, preferably 20mm.

Specifically, the box body 2 is a generally square overall structure, which is composed of several internal cavities, micro-pipes, via holes, etc., and at least a magnetic bead cavity 101, an extraction cavity 102, a waste liquid cavity 103, and a sample cavity are arranged inside. 104 , first cleaning chamber 105 , second cleaning chamber 106 , elution chamber 107 , reagent chamber 108 , spare chamber 109 , installation hole 1012 , wherein the sampling tube 1 is connected to the sample chamber 104 .

As shown in Figures 2 and 3, the box body 2 includes a box body 22 and an upper cover plate 21 covering the top of the box body 22, several cavities described above are arranged inside the box body 22, and the lower end surface of the box body 22 ( The reverse side) is provided with a number of first micro-pipes 221 of different sizes, and the first micro-pipes 221 communicate with the corresponding cavities sequentially through a number of via holes, so that each group of cavities and the corresponding first micro-pipes 221 are independent of each other. No interference, forming an independent channel;

Specifically, the upper cover plate 21 is a flat plate provided with a number of feeding holes 211 corresponding to the cavity, and the upper end surface of the upper cover plate 21 is covered with a top sealing film 212 for sealing. In a preferred embodiment, the feed hole 211 corresponding to the cavity containing the volatile liquid is configured with a sealing cork 213 before covering the top sealing film 212 to prevent the liquid from volatilizing, such as the magnetic bead cavity 101, the cleaning cavity 105 and The second chamber 106 is cleaned.

Further, the box body 2 also includes a lower cover plate 23 covering the lower side of the box body 22. The lower cover plate 23 is a flat plate, divided into two sides, the front side is upward, and an amplification detection area is provided, including the amplification detection area. The detection array 121 (through the upper and lower sides), the detection micropipe 122 communicated with the amplification detection array, and the upper end surface of the amplification detection area covers the detection area sealing film 123 for sealing. Downward is the reverse side, which is provided with a number of second micro-pipes 231 of different sizes, wherein the front and back sides pass through the second micro-pipes 231 through a number of via holes, and communicate with the detection micro-pipes 122, so that each group of second micro-pipes 231 They are independent of each other and do not interfere with each other, forming an independent channel. The lower end surface (reverse surface) of the lower cover plate 23 covers the bottom sealing film 232 for sealing the second micropipe 231 and the amplification detection area.

The fluid channel is divided into a first-layer fluid channel and a second-layer fluid channel, and the second micro-pipe 231 cooperates with the bottom sealing film 232 to form the second-layer fluid channel, while the upper end surface (front side) of the first micro-pipe 221 and the lower cover plate 23 ) cooperate to form a first-layer fluid channel, and the first-layer fluid channel and the second-layer fluid channel cooperate to form a channel that can communicate between cavities. In one embodiment, the second micropipe 231 is provided with several fluid state cavities 2311, and the box body 22 is provided with fluid observation windows 2312 corresponding to the fluid state cavities 2311, so as to dynamically observe the fluid state during the reaction.

Further, the nucleic acid detection cartridge also includes a rotating mechanism 3, which is composed of a limit ring 31, a rotary valve 32, a cushion 33, and a limit wall 34. The limit ring 31 and the limit wall 34 enclose a rotation space.

Specifically referring to Fig. 14 and Fig. 15, the soft pad 33 adopts a smooth and elastic material, such as elastomer materials such as styrene, styrene-isoprene-styrene block copolymer, polypropylene, thermoplastic polyurethane, etc. production. A plurality of positioning holes 331 are provided on the lower end surface, and the positioning holes 331 cooperate with a plurality of positioning columns 332 provided on the box body 22 to fix the rotating mechanism 3 . The soft pad 33 is also provided with a number of through holes that penetrate up and down, and the positions of the through holes are in one-to-one correspondence with the through holes on the corresponding positions of the box body 22, so as to realize the through holes.

Referring specifically to Fig. 12 and Fig. 13, the rotary valve 32 is a whole and is the only moving part in the rotary mechanism 3. The lower end surface of the rotary valve 32 is provided with two long grooves 321 and short grooves 322 of different lengths. The rotary valve 32 can be rotated so that the long groove 321 cooperates with the through hole on the cushion 33 to form a liquid channel, and the liquid channel can be selectively connected to each cavity; similarly, the rotary valve 32 can also be rotated to make the short The groove 322 cooperates with the through hole on the cushion 33 to form a gas channel, and the gas channel can also be selectively connected to each cavity.

Referring to Figure 16 and Figure 17 specifically, the lower end surface of the limiting ring 31 is set as a bonding area 311 and a smooth area 312, the bonding area 311 and the upper surface of the limiting wall 34 are completely fixed using a bonding process, and the smooth area 312 will be pressed down Rotating the valve 32 forces the lower surface of the rotating valve 32 to press down on the cushion 33 to deform and form a certain pre-tightening force to play a sealing role.

Further, the box body 22 is also provided with a gas inlet and outlet channel, including the air hole A1010, the air hole B1011 and the waste liquid chamber air outlet C1013, the air hole A1010 and the air hole B1011 run through the upper cover plate 21 and the top sealing film 212, and the waste liquid chamber air outlet C1013 is connected waste chamber. Through the rotation of the rotary valve 32, the air hole A1010 or the air hole B1011 can be connected to the gas flow channel, and the air hole A1010 or the air hole B1011 can also be connected to the extraction chamber or the reagent chamber; and gas outlet, so as to provide gas power to control the flow direction of the substances used for nucleic acid extraction in each chamber. The lower port of the gas inlet and outlet channel, that is, the position of the lower end surface of the corresponding box body 22 is covered with a waterproof and breathable membrane 1014, which is used to isolate the internal and external environment of the detection cartridge, and has the function of blocking liquid and ventilation, ensuring the integrity of the detection cartridge. Organic or harmful substances such as liquids and aerosols, as well as interfering substances in the external environment of the detection cartridge cannot enter and exit, avoiding pollution and interference.

The invention also discloses an assembly process of a nucleic acid detection cartridge, comprising the following steps:

Step 1: The bottom sealing film 232 is bonded to the lower cover plate 23 to form a second layer of fluid channels;

Step 2: Cover the waterproof and breathable membrane 1014 to the corresponding gas inlet and outlet channel positions on the lower end surface of the box body 22;

Step 3: The lower cover plate 23 is bonded to the lower end surface of the box body 22 to form a first-layer fluid channel;

Step 4: The box body 22 is bonded with the upper cover 21 to form a complete cavity;

Step 5: Assemble the rotating mechanism 3:

First soft pad 33 is packed in the spacer wall 34, and then the positioning column 332 on the box body 22 cooperates with the positioning hole 331 on the soft pad 33 to connect and fix the position, and is close to the bottom surface to ensure that the via hole on the soft pad 33 is in line with the bottom surface. The via holes on the upper end surface of the box body 22 in the limiting wall 34 correspond one by one; The bonding process is used to completely fix, and at the same time, the smooth area 312 on the lower end surface of the limit ring 31 presses down on the rotary valve 32, forcing the lower surface of the rotary valve 32 to press down on the cushion 33 to produce deformation, forming a certain pre-tightening force, and playing a sealing role;

Step 6: Add liquid, add the substances used for nucleic acid extraction one by one through the feeding hole 211 of the upper cover plate 21, seal the feeding hole 211 with a sealing plug 213 for the volatile liquid, and then use the top sealing film 212 to bond with the upper cover plate 21 sealing;

Step 7: Add reagents to the reagent chamber, and seal it with the reagent chamber cover 214;

Step 8: Add primers to the amplification detection array, and use the detection area sealing film 123 to bond and seal.

The bonding described in the above steps refers to the fixed bonding of two same or different materials into one, such as pressure-sensitive adhesives, photosensitive adhesives, heat-sensitive adhesives, double-sided tape, thermal bonding, ultrasonic bonding, etc. Bonding, laser bonding, bump-to-groove coupling, etc.

The hard material and film material used in the detection cartridge can be made of polymer materials such as polypropylene, cycloolefin copolymer, cycloolefin polymer, polymethyl methacrylate, polystyrene or polycarbonate.

For ease of understanding, the method of using the nucleic acid detection cartridge in the present invention is as follows:

Step 1: Rotate the rotary valve 32, the extraction chamber and the magnetic bead chamber are connected through the fluid channel, the gas outlet from the air hole A1010, the air intake from the air hole B1011, the liquid in the magnetic bead chamber enters the extraction chamber, and the external magnet absorbs the magnetic beads after mixing;

Step 2: Rotate the rotary valve 32, the extraction chamber and the waste liquid chamber are communicated through the fluid channel, the air intake through the air hole A1010, the air out through the waste liquid chamber outlet C1013, and the waste liquid in the extraction chamber is discharged into the waste liquid chamber;

Step 3: Rotate the rotary valve 32, the extraction chamber and the sample chamber are connected through the fluid channel, the gas outlet from the air hole A1010, the air intake from the air hole B1011, the sample in the sample chamber enters the extraction chamber, the sample is mixed with the magnetic beads, and the magnetic beads absorb the nucleic acid in the sample After that, the external magnet absorbs the magnetic beads;

Step 4: Repeat Step 2;

Step 5: Rotate the rotary valve 32, the extraction chamber and the first cleaning chamber are connected through the fluid channel, the gas outlet from the air hole A1010, the air intake from the air hole B1011, the cleaning solution in the first cleaning chamber enters the extraction chamber, and after the nucleic acid adsorbed by the magnetic beads is cleaned, External magnet absorbs magnetic beads;

Step 6: Repeat Step 2;

Step 7: Rotate the rotary valve 32, the extraction chamber and the second cleaning chamber are connected through the fluid channel, the gas outlet of the air hole A1010, the air intake of the air hole B1011, the cleaning liquid in the second cleaning chamber enters the extraction chamber, and after the nucleic acid adsorbed by the magnetic beads is cleaned, The external magnet absorbs the magnetic beads;

Step 8: Repeat step 2;

Step 9: Rotate the rotary valve 32, the extraction chamber and the elution chamber are connected through the fluid channel, the gas outlet of the air hole A1010, the air intake of the air hole B1011, the eluent in the elution chamber enters the extraction chamber, the eluent is mixed with the magnetic beads, and the After the target nucleic acid substance adsorbed by the magnetic beads is eluted, the external magnet absorbs the magnetic beads;

Step 10: Rotate the rotary valve 32, the extraction chamber and the reagent chamber are connected through the fluid channel, the air is fed into the air hole A1010, and the air is discharged from the air hole B1011, and the eluent mixed with the target nucleic acid substance enters the reagent chamber and mixes with the freeze-dried reagent in the reagent chamber ; After the mixing is completed, air is released from the air hole A1010 and air is taken in from the air hole B1011, the external magnet remains in the adsorption state, and the mixed reagent in the reagent chamber enters the extraction chamber;

Step 11: Rotate the rotary valve 32, the extraction chamber and the amplification detection array are connected through the fluid channel, the air is fed into the air hole A1010, and the air is discharged from the air hole B1011, and the mixed reagent solution in the extraction chamber enters the amplification detection array;

Step 12: Rotate the rotary valve 32 so that the amplification detection area is completely closed, and start nucleic acid amplification and detection.

Although the present invention has been described in detail with general descriptions and specific examples above, it is obvious to those skilled in the art that some modifications or improvements can be made on the basis of the present invention. Therefore, the modifications or improvements made on the basis of not departing from the spirit of the present invention all belong to the protection scope of the present invention. The embodiments of the present invention described above are not intended to limit the protection scope of the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principle of the present invention shall be included in the protection scope of the claims of the present invention.

Claims (13)

A nucleic acid detection cartridge, characterized in that it includes a sampling tube (1) and a box body (2), and the box body (2) is provided with several cavities for containing materials used for nucleic acid extraction and a cavity communicating with the cavities. A plurality of fluid channels, the sampling tube (1) communicates with one of the cavities, and the fluid channels can be selectively communicated with each other. A nucleic acid detection cartridge according to claim 1, characterized in that the box body (2) comprises a box body (22) and an upper cover (21) covering the box body (22), The upper cover plate (21) is provided with a feeding hole (211) corresponding to the cavity, and the upper end surface of the upper cover plate (21) is covered with a top sealing film (212) for sealing. The nucleic acid detection cartridge according to claim 2, characterized in that the feeding hole (211) corresponding to the cavity containing the volatile substances is equipped with a sealing cork (213). A nucleic acid detection cartridge according to claim 1, wherein the fluid channel is divided into a first layer of fluid channel and a second layer of fluid channel, and the lower end surface of the box body (22) is provided with several first A micropipe (221), the first micropipe (221) communicates with the cavity through a via hole; The box body (2) also includes a lower cover plate (23) covered below the box body (22), the lower end surface of the lower cover plate (23) is provided with some second micro-ducts (231), the The bottom surface of the lower cover plate (23) covers the bottom sealing film (232) for sealing, and the second micropipe (231) cooperates with the bottom sealing film (232) to form a second layer of fluid channels, and the first The micropipe (221) cooperates with the upper end surface of the lower cover plate (23) to form a first layer of fluid channels. A nucleic acid detection cartridge according to claim 4, wherein an amplification detection area is also set on the lower cover plate (23), and the amplification detection area includes an amplification detection array (121) and a The amplification detection array (121) communicates with the detection micropipe (122), and the upper end of the amplification detection area covers the detection area sealing film (123) for sealing. A nucleic acid detection cartridge according to claim 4, characterized in that, the second micropipe (231) is provided with several fluid state cavities (2311), and the cartridge body (22) is provided with The fluid observation window (2312) corresponding to the state chamber (2311). A nucleic acid detection cartridge according to claim 1, characterized in that, the nucleic acid detection cartridge further comprises a rotating mechanism (3), and the rotating mechanism (3) controls the selectable flow between the fluid channels through rotation. Connect sexually. A nucleic acid detection cartridge according to claim 7, characterized in that, the rotating mechanism (3) comprises a soft pad (33) and a rotary valve (32) connected in sequence, and the bottom of the soft pad (33) The end face is provided with several positioning holes (331), and the positioning holes (331) cooperate with several positioning columns (332) provided on the box body (2) to fix the rotating mechanism (3), and the soft pad (33 ) are respectively connected to the fluid channel through a plurality of via holes, the lower end surface of the rotary valve (32) is provided with a liquid flow channel and a gas flow channel, and the liquid flow channel can selectively communicate with the fluid channel through the via holes, The gas channel can selectively communicate with the fluid channel through the via hole. A nucleic acid detection cartridge according to claim 8, characterized in that, the rotating mechanism (3) further comprises a limit ring (31) and a limit wall (34), and the limit ring (31) The end face is set as a bonding area (311) and a smooth area (312), the smooth area (312) is pressed against the edge of the rotary valve for sealing, and the bonding area (311) is connected to the limiting wall ( 34), the limit ring (31) cooperates with the limit wall (34) to form a rotation space. A nucleic acid detection cartridge according to claim 8, characterized in that, the box body (2) is also provided with several gas inlet and outlet channels, and the gas inlet and outlet channels can be selectively connected to the gas flow channel Or the cavity, the lower port of the gas inlet and outlet channel is covered with a waterproof and breathable membrane (1014), and the gas inlet and outlet channel controls the flow direction of the substances used for nucleic acid extraction by providing gas power. A nucleic acid detection cartridge according to claim 1, characterized in that, the length range of the box body (2) is 50mm-70mm, and/or the width range is 20mm-40mm, and/or the height range is 15mm -30mm. A kind of assembly process of nucleic acid detection cartridge, it is characterized in that, described assembly process comprises the following steps: Step 1: The lower cover plate (23) is bonded to the box body (22) to form a fluid channel; Step 2: The box body (22) is bonded with the upper cover (21) to form a complete cavity; Step 3: Assemble the rotating mechanism (3), put the cushion (33) into the limiting wall (34) to fix the rotating mechanism (3), and ensure that the through hole on the cushion (33) is in line with the The through holes at the corresponding positions on the upper end surface of the box body (22) correspond one by one, and then the rotary valve (32) is installed above the soft pad (33); Step 4: adding liquid, adding substances used for nucleic acid extraction into the cavity one by one, and then sealing; Step 5: Add primers to the amplification detection array and seal it. The assembly process of a nucleic acid detection cartridge according to claim 12, wherein the assembly process in step 1 comprises: First, the bottom sealing film (232) is bonded to the lower cover plate (23) to form a second layer of fluid channels; Secondly, the waterproof gas-permeable membrane (1014) is covered to the position of the gas inlet and outlet channel on the lower end surface of the box body (22); Again, the lower cover plate (23) is bonded to the box body (22) to form the first layer of fluid channels.

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