WO2023000408A1 - COMPOSITION COMPRISING β-NICOTINAMIDE MONONUCLEOTIDE AND ROXBURGH ROSE EXTRACT, AND APPLICATION THEREOF - Google Patents

Abstract

A composition comprising β-nicotinamide mononucleotide (NMN) and a roxburgh rose extract, and an application thereof, relating to the technical field of biomedicine. Provided are a composition comprising β-NMN and a roxburgh rose extract, and an application thereof. An active ingredient of the composition is composed of the following components in percentage by weight: β-NMN: 45-6%, and the remainder being the roxburgh rose extract. By compounding NMN with the roxburgh rose extract, the intestinal flora disorder is effectively improved, and the aging is delayed while reducing the amount of NMN.

Description

Composition comprising β-nicotinamide mononucleotide and Rosa roxburghii extract and application thereof technical field

The invention relates to a composition comprising β-nicotinamide mononucleotide and Rosa roxburghii extract and application thereof, belonging to the technical field of biomedicine.

Background technique

There are various and large numbers of microbial flora in the human intestinal tract. They interact to form a complex and relatively stable micro-ecosystem, and participate in many processes such as human immune regulation, biological antagonism, tumor suppression, lipid metabolism, and nutrient absorption. Human health plays a very important role. There are more than 1,000 types of microbial flora in the human gut, ten times the number of human cells. Under normal circumstances, the intestinal flora will maintain a relatively balanced state, but under the stimulation of some factors, it will lead to the imbalance of intestinal flora, which will lead to a variety of diseases, such as obesity, Alzheimer's disease, tumors, depression Wait. There are many factors that cause flora imbalance, including long-term and large-scale use of antibiotics, constipation, diet, living habits, environmental factors, and mental factors.

At present, the main way to improve the intestinal flora is to supplement probiotics. However, the live bacteria used in this method reach the intestinal tract through extreme environments such as gastric acid and bile after production, sale on the shelf, and consumption. Increase.

β-nicotinamide mononucleotide (abbreviated as NMN) is a naturally occurring bioactive nucleotide, which has important physiological functions for human cells, can be synthesized naturally in cells, and can also be derived from a variety of foods such as Western Orchids, cabbage, cucumbers, edamame, etc. Chinese patent CN111557949A discloses a composition of hydrogen-rich water and NMN. The results of this patent show that NMN, given to HFA mice at a dose of 22g/kg/day for 30 days, can improve intestinal bacteria to a certain extent. The role of the group. It can be seen that if NMN is used alone to improve the intestinal flora, the dosage is too large, resulting in high cost and affecting the promotion of use.

Rosa roxburghii is a natural medicinal and edible plant, which is recorded in "Compendium of Materia Medica" and "Dictionary of Chinese Medicine". Rosa roxburghii fruit is rich in nutrients such as flavonoids, polysaccharides, vitamins, superoxide dismutase (SOD) and organic acids, and has the functions of regulating immune function, delaying aging, anti-atherosclerosis and anti-tumor. Studies have shown that Rosa roxburghii polysaccharides can increase the abundance of beneficial bacteria and reduce the abundance of harmful bacteria in the intestinal tract of diabetic db/db mice to a certain extent, but have no significant effect on the diversity index and abundance of intestinal microorganisms (Wang et al. , Journal of Functional Foods, 2019, 52:408–417).

Contents of the invention

In view of the above defects, the technical problem to be solved by the present invention is to provide a composition, which can improve the intestinal flora and anti-aging ability through the combination of β-nicotinamide mononucleotide and Rosa roxburghii extract.

The composition of the present invention comprises β-nicotinamide mononucleotide and Rosa roxburghii extract, and its active ingredient is composed of the following components by weight percentage: β-nicotinamide mononucleotide is 45-65%, and the rest is Rosa roxburghii extract things.

In one embodiment of the present invention, the active ingredient of the composition comprising β-nicotinamide mononucleotide and Rosa roxburghii extract consists of the following components in weight percentage: β-nicotinamide mononucleotide 50～ 60%, and the rest is prickly pear extract.

In a specific embodiment of the present invention, the active ingredient of the composition comprising β-nicotinamide mononucleotide and Rosa roxburghii extract consists of the following components by weight percentage: β-nicotinamide mononucleotide 50% , and the rest is Rosa roxburghii extract.

In one embodiment of the present invention, the Rosa roxburghii extract described in the present invention is an aqueous extract.

In one embodiment of the present invention, the present invention comprises a composition comprising β-nicotinamide mononucleotide and Rosa roxburghii extract, and also includes food acceptable excipients.

In one embodiment of the present invention, the composition comprising β-nicotinamide mononucleotide and Rosa roxburghii extract is granule, tablet, capsule, pill, ointment, drink or biscuit.

The present invention also provides the application of the composition comprising β-nicotinamide mononucleotide and Rosa roxburghii extract in the preparation of health care products or medicines for improving intestinal flora disturbance.

The composition comprising β-nicotinamide mononucleotide and Rosa roxburghii extract can improve intestinal flora, increase the number of lactobacilli and bifidobacteria, and help to improve intestinal flora disorder.

The present invention also provides the application of the composition comprising β-nicotinamide mononucleotide and Rosa roxburghii extract in the preparation of anti-aging health products or medicines.

The composition of the invention comprising β-nicotinamide mononucleotide and roxburghii extract can prolong the lifespan of nematodes and delay aging.

Compared with the prior art, the present invention has the following beneficial effects:

The invention combines NMN and Rosa roxburghii extract, and the two synergize to significantly improve intestinal flora and prolong the lifespan of nematodes, effectively improve intestinal flora disorder and delay aging. In addition, the composition of the invention has low cost and is easy to popularize.

Description of drawings

Fig. 1 is the community composition analysis (mean value) figure in Test Example 2 of the present invention, wherein, A figure is the relative abundance of intestinal flora on the phylum level; B figure is the relative abundance of intestinal flora on the genus level.

Fig. 2 is an Alpha diversity analysis diagram of the mouse ileocecal flora in Test Example 2 of the present invention.

Fig. 3 is a PCoA analysis diagram of the Beta diversity of the mouse ileocecal flora in Test Example 2 of the present invention.

detailed description

The composition of the present invention comprises β-nicotinamide mononucleotide and Rosa roxburghii extract, and its active ingredient is composed of the following components by weight percentage: β-nicotinamide mononucleotide is 45-65%, and the rest is Rosa roxburghii extract thing.

The present invention achieves the purpose of mutual synergy through the specific ratio of β-nicotinamide mononucleotide and Rosa roxburghii extract, thereby reducing the amount of NMN while enhancing the ability of the composition to improve intestinal flora and anti-aging .

In one embodiment of the present invention, the active ingredient of the composition comprising β-nicotinamide mononucleotide and Rosa roxburghii extract consists of the following components in weight percentage: β-nicotinamide mononucleotide 50～ 60%, and the rest is prickly pear extract.

In a specific embodiment of the present invention, the active ingredient of the composition comprising β-nicotinamide mononucleotide and Rosa roxburghii extract consists of the following components by weight percentage: β-nicotinamide mononucleotide 50% , and the rest is Rosa roxburghii extract.

The Rosa roxburghii extract of the present invention is an aqueous extract. The Rosa roxburghii extract is rich in SOD, Rosa roxburghii polysaccharides, Rosa roxburghii polyphenols or vitamin C, and commercially available Rosa roxburghii extracts can be used.

The invention comprises the composition of β-nicotinamide mononucleotide and roxburghii extract, and also includes food acceptable auxiliary materials.

The dosage forms commonly used in this field can be prepared by adding auxiliary materials. In one embodiment of the present invention, the composition comprising β-nicotinamide mononucleotide and Rosa roxburghii extract is granule, tablet, capsule, pill, ointment, drink or biscuit.

The composition of the present invention comprising β-nicotinamide mononucleotide and Rosa roxburghii extract can be prepared by conventional methods. In one embodiment of the present invention, it can be prepared by the following method:

Mix β-nicotinamide mononucleotide, Rosa roxburghii extract and auxiliary materials uniformly to obtain the composition containing β-nicotinamide mononucleotide and Rosa roxburghii extract.

The composition comprising β-nicotinamide mononucleotide and Rosa roxburghii extract of the present invention can be used in the preparation of health products or medicines for improving intestinal flora disturbance.

The composition comprising β-nicotinamide mononucleotide and Rosa roxburghii extract can improve intestinal flora, increase the number of lactobacilli and bifidobacteria, and help to improve intestinal flora disorder.

The composition comprising β-nicotinamide mononucleotide and Rosa roxburghii extract of the present invention can be used in the preparation of anti-aging health care products or medicines.

The composition of the invention comprising β-nicotinamide mononucleotide and roxburghii extract can prolong the lifespan of nematodes and delay aging.

The specific implementation of the present invention will be further described below in conjunction with the examples, and the present invention is not limited to the scope of the examples. Rosa roxburghii extract in the examples was purchased from Xi'an Ruilin Biotechnology Co., Ltd., and β-nicotinamide mononucleotide was self-made.

Example 1

This embodiment provides a composition, the total mass of the composition is 50g, wherein the mass fraction of the main components of the composition is: 50% of β-nicotinamide mononucleotide, 50% of Rosa roxburghii extract, weighed in proportion Two ingredients, mix well.

Example 2

This embodiment provides a composition, the total mass of the composition is 50g, wherein the mass fraction of the main components of the composition is: 60% of β-nicotinamide mononucleotide, 40% of Rosa roxburghii extract, weighed in proportion Two ingredients, mix well.

Comparative example 1

This comparative example provides a composition, the total mass of the composition is 50g, and the mass fraction of the main components of the composition is: 10% of β-nicotinamide mononucleotide, 90% of Rosa roxburghii extract, weighed in proportion Two ingredients, mix well.

Comparative example 2

This comparative example provides a composition, the total mass of the composition is 50g, and the mass fraction of the main components of the composition is: 20% of β-nicotinamide mononucleotide, 80% of Rosa roxburghii extract, weighed in proportion Two ingredients, mix well.

Comparative example 3

This comparative example provides a composition, the total mass of the composition is 50g, wherein the mass fraction of the main components of the composition is: 30% of β-nicotinamide mononucleotide, 70% of Rosa roxburghii extract, weighed in proportion Two ingredients, mix well.

Comparative example 4

This comparative example provides a composition, the total mass of the composition is 50g, and the mass fraction of the main components of the composition is: 40% of β-nicotinamide mononucleotide, 60% of Rosa roxburghii extract, weighed in proportion Two ingredients, mix well.

Comparative example 5

This comparative example provides a composition, the total mass of the composition is 50g, wherein the mass fraction of the main components of the composition is: 70% of β-nicotinamide mononucleotide, 30% of Rosa roxburghii extract, weighed in proportion Two ingredients, mix well.

Comparative example 6

This comparative example provides a composition, the total mass of the composition is 50g, wherein the mass fraction of the main components of the composition is: 80% of β-nicotinamide mononucleotide, 20% of Rosa roxburghii extract, weighed in proportion Two ingredients, mix well.

Comparative example 7

This comparative example provides a composition, the total mass of the composition is 50g, wherein the mass fraction of the main components of the composition is: 90% of β-nicotinamide mononucleotide, 10% of Rosa roxburghii extract, weighed in proportion Two ingredients, mix well.

Test Example 1 Effect of composition on intestinal flora of human flora (HFA) mouse model

(1) Construction of HFA mouse model

Choose a healthy volunteer, female, 25 years old, who has not taken any antibiotics within 3 months. Collect fresh feces from volunteers for the first time in the morning, weigh the feces under aseptic and anaerobic conditions and add them to Wilkins-Chalgren anaerobic broth (feces: broth = 1g: 9mL), shake and mix to prepare The stool suspension is ready for use. Take 4-week-old KM mice, weighing 22±3g, half male and half male. Each KM mouse was intragastrically administered with 0.3 mL of the above-mentioned feces suspension, cultured for 21 days, and the HFA mouse model was obtained after colonization of human intestinal flora in the mice. HFA mice were bred in SPF animal room, fed with sterile mouse-based feed and sterile water, and had free access to food and water during the experiment.

(2) Effect of composition on intestinal flora of HFA model

The HFA mice were randomly divided into 10 groups, and a blank solvent control group, a β-nicotinamide mononucleotide group, a Rosa roxburghii extract group, and the composition groups of Examples 1-2 and Comparative Examples 1-7 were set up. The dosages were all 100mg/kg, the samples were dissolved in normal saline, and 0.3mL was administered to each mouse. During the experiment, the mice were given regular gavage every day, and the blank solvent control group was gavaged with the same volume of normal saline every day for 21 consecutive days. During the feeding period, the mice were free to eat and drink. During the experiment, the body weight changes of the mice in each group were recorded. After the mice were killed on the 22nd day, the contents of the cecum were collected aseptically, and the contents were placed in a sterile test tube. (g/V) was mixed with sterile physiological saline, and oscillated on a shaker for 15 minutes to obtain a suspension of intestinal contents. Take 6 test tubes containing 9 mL of sterilized physiological saline, and dilute the contents of the cecum in turn until the dilution factor is 10 ^-5 . The dilution of the sample was based on Bifidobacteria (10 ^-3 , 10 ^-4 , 10 ^-5 ), Lactobacillus (10 ^-3 , 10 ^-4 , 10 ^-5 ), Enterobacteriaceae (10 ^-2 , 10 ^-3 , 10 ^{- 4} ), enterococci (10 ^-2 , 10 ^-3 , 10 ^-4 ), take 0.2mL samples of different dilutions with a pipette on different selection media, spread and culture, each dilution Culture medium to do 3 parallel plates. The culture conditions and identification methods of different intestinal flora are shown in Table 1.

Table 1 Culture conditions and identification methods of intestinal flora

After the culture was completed, the conventional plate viable count method was used to count, and the average number of colonies α of the same dilution of various colonies was calculated, and the total number of colonies of samples with three dilutions of various colonies were calculated according to the formula, and the final result was expressed as colonies per gram of feces The logarithmic representation of the number. When counting, select a plate with 30-300 plate colonies.

Total number of colonies [l g (CFU/g)] = α × dilution factor / 0.1

The body weight changes of the mice before and after the experiment and the number of bacteria in the intestinal tract of the mice are shown in Table 2.

Table 2 Changes in body weight of mice before and after the experiment and the number of bacteria in the intestinal tract of mice (n=10)

Note: Compared with the solvent control group, *P<0.05, **P<0.01, ***P<0.001

It can be seen from Table 2 that, compared with the solvent control group, the body weight of the mice increased at the end of the experiment. Compared with solvent group, NMN group and embodiment 1 composition group, mouse body weight is obviously lower than solvent group, show that NMN and embodiment 1, the composition of embodiment 2 have obvious weight-reducing effect, and embodiment 1 and implementation The weight loss effect of the composition of Example 2 is better than that of NMN. In terms of improving the intestinal flora, compared with the solvent group, at 100mg/kg, NMN had no significant effect on the intestinal flora of HFA mice, while Rosa roxburghii extract could increase the number of Lactobacillus and Bifidobacteria, and reduce the number of Enterobacteriaceae and the number of enterococci. When NMN was combined with Rosa roxburghii extract, with the increase of the ratio of Rosa roxburghii extract, the number of Lactobacillus and Bifidobacteria in the intestinal tract first increased and then decreased, while the number of Enterobacteriaceae and Enterococcus in the intestinal tract decreased first After the increasing trend, especially the compositions of Example 1 and Example 2, compared with the solvent group, the difference in the increase in the number of lactobacilli and bifidobacteria is extremely significant. It can be seen that the combination of NMN and Rosa roxburghii extract has a synergistic effect, which has an unexpected improvement effect on the intestinal flora of HFA model mice.

Test Example 2 Reconstitution Effect of Composition on Antibiotic-Induced Intestinal Flora Disorder

According to Test Example 1, the composition of Example 1 has a significant improvement effect on the intestinal flora of HFA model mice. This test example will further investigate the reconstruction of the composition of Example 1 on the intestinal flora disorder induced by antibiotics effect.

(1) Establishment of a mouse intestinal flora dysbiosis model

Take 4-week-old KM mice, half male and half female, weighing 22±3g, and start the experiment after one week of adaptive feeding. 5 female mice and 5 male mice were randomly left as the normal control group (Nor group), and the rest were given lincomycin hydrochloride solution by intragastric administration, 12 mL/kg, twice a day for 7 consecutive days. Give an equal volume of normal saline. After 7 days, 5 female mice and 5 male mice with diarrhea were respectively selected and killed as the model group (Mod group). The feces from the ileocecum of the mice were collected under sterile conditions, and the feces were observed for 16S rRNA detection. .

(2) Grouping of mice and drug treatment

Randomly group the mice with diarrhea, 10 in each group, set up the natural recovery group (Con), β-nicotinamide mononucleotide group (NMN, 100mg/kg), and Rosa roxburghii extract group (CL, 100mg/kg) And embodiment 1 composition high-dose (200mg/kg) group [NMN+CL (H)] and low-dose group (100mg/kg) [NMN+CL (L)], dissolve composition with physiological saline, each fill Stomach 0.3mL. During the experiment, the mice were given regular gavage every day, and the natural recovery group was gavaged with the same volume of normal saline every day for 7 consecutive days. During the feeding period, the mice were free to eat and drink. During the experiment, the weight changes of the mice in each group were recorded. After the mice were killed on the 8th day, the feces from the ileocecum of each group were aseptically collected, and the feces properties were observed, and 16S rRNA was detected.

(3) High-throughput sequencing of intestinal microorganisms and analysis of their flora

Use a specific DNA extraction kit to extract total genomic DNA from mouse feces samples. After detecting DNA integrity and concentration, the 16S rDNA V4 region of the sample is amplified. The amplification primer sequence is 515F (5'-GTGYCAGCMGCCGCGGTAA-3 ') and 806R (5'-GGACTACHVGGGTWTCTAAT-3'). After amplification, the target fragments are detected by electrophoresis, and the qualified samples are collected from the target bands for recovery, and a library is constructed for high-throughput sequencing. The sequencing results were spliced and filtered to obtain high-quality target sequences, and bioinformatics analysis was performed, including community composition analysis, Alpha diversity analysis, Beta diversity analysis, etc. The results are shown in Figures 1 to 3.

It can be seen from A in Figure 1 that at the phylum level, Bacteroidetes, Firmicutes and Proteobacteria were the main phyla in the ileocecum of the normal group. After administration of lincomycin, the abundance of Bacteroidetes in the ileocecum of mice decreased sharply, and the abundance of Proteobacteria and Firmicutes increased significantly. The abundance of Bacteroidetes, Firmicutes and Proteobacteria recovered after feeding with NMN or Rosa roxburghii extract. After giving the composition of embodiment 1, low dosage group (100mg/kg) [NMN+CL (L) and high dosage group (200mg/kg) [NMN+CL (H) all can make Bacteroidetes, thick The abundance of Bicobacteria and Proteobacteria recovered significantly, the abundance of Bacteroidetes increased, and the abundance of Proteobacteria and Firmicutes decreased. It shows that the combination of NMN and Rosa roxburghii extract effectively increases the content of beneficial bacteria in the intestinal tract of mice with dysbiosis, inhibits harmful bacteria, and makes the composition of intestinal flora tend to normal levels.

It can be seen from B of Figure 1 that after treatment with lincomycin, at the genus level, Bacteroides, Parabacteroides, Ekmansia, Enterobacter and Lachnospira in the ileocecum of mice The abundance of Staphylococcus and Pseudomonas significantly increased. The abundance of Bacteroides and Ekmansia in the ileocecum was partially increased after administration of NMN and Rosa roxburghii extract. After giving the composition of embodiment 1, low dosage group (100mg/kg) [NMN+CL (L) and high dosage group (200mg/kg) [NMN+CL (H) all can make beneficial bacteria Bacteroides , Parabacteroides, Ekmania, and Lachnospira significantly increased, while the abundance of harmful bacteria such as Pseudomonas and Staphylococcus was significantly suppressed. It shows that the composition of NMN and Rosa roxburghii can effectively promote the beneficial bacteria in the intestinal tract of mice and inhibit the harmful bacteria in the intestinal tract.

Figure 2 shows the results of Alpha diversity analysis of mouse ileocecal flora. It can be seen from Figure 2 that after administration of lincomycin, the abundance and diversity of intestinal flora were significantly lower than those in normal mice. After administration of NMN and Rosa roxburghii extract, the abundance and diversity of the flora increased compared with the model group. After the composition of Example 1 is given, the low-dose group (100mg/kg) [NMN+CL (L) and the high-dose group (200mg/kg) [NMN+CL (H) can make the flora abundance and diversity Significantly improved sex.

Figure 3 is the Beta diversity analysis of the ileocecal flora of mice. It can be seen from Figure 3 that after lincomycin treatment, the flora structure is significantly different from that of the normal group, and the flora diversity is reduced. After administration of NMN and Rosa roxburghii extract, the diversity of flora increased. After the composition of Example 1 is given, the low-dose group (100mg/kg) [NMN+CL (L) and the high-dose group (200mg/kg) [NMN+CL (H) can significantly improve the diversity of flora .

In summary, the combination of NMN and Rosa roxburghii has a restoration and reconstruction effect on the intestinal flora of mice with antibiotic-induced flora dysbiosis.

Test Example 3 Composition anti-aging activity test

(1) Experimental materials

Experimental nematode strain: wild-type nematode strain C.elegans Bristol N2

Experimental strain: uracil-deficient Escherichia coli (OP50), which cannot synthesize uracil by itself, and the uracil required for its own growth needs to be taken from solid medium.

Experimental medicinal solution and preparation method: take NMN, roxburghii extract and the compositions of Example 1, Example 2 and Comparative Examples 1-7, add physiological saline to dissolve, and use a 0.22 μm water-based microporous filter membrane in an ultra-clean workbench Filter sterilize. Dispense into 1mL sterile EP tubes, seal with parafilm, and store in the refrigerator for later use. When in use, open the medicinal solution in an ultra-clean workbench, shake well, and dilute it with medium to a concentration of 100, 250 and 500 mg/mL.

(2) Experimental method of Caenorhabditis elegans

This experiment is divided into an experimental group and a control group. The synchronized Caenorhabditis elegans is cultured to the young adult stage in a biochemical incubator, and C. C. elegans to the NGM 6-well plate coated with OP50 bacteria, about 150 Caenorhabditis elegans per well, try to keep the size of the worm body consistent and the number of the experimental group and the control group should be consistent. In the experimental group, a layer of medicinal solution was spread on the NGM plate coated with OP50 bacteria, and in the control group, the culture medium was spread flat. At this time, the lifespan of the nematode was recorded on the first day, and Caenorhabditis elegans should be observed and transferred to the new plant every day for the first seven days. elegans in a clean NGM 6-well plate to ensure that the living conditions of C. elegans are good, the food is sufficient, and there is no contamination by bacteria. After the seventh day, it can be transferred every other day, because at this time, C. elegans is almost no longer able to reproduce. At this time, it is necessary to observe the growth of Caenorhabditis elegans every day and record the number of deaths of Caenorhabditis elegans per day, including normal death and abnormal death, and organize the data. The method of judging the death of Caenorhabditis elegans: 1. Gently touch the body of Caenorhabditis elegans with a platinum wire, and observe whether the Caenorhabditis elegans responds. 2. Observe under the microscope whether the pharynx of C. elegans twitches. The experimental results are shown in Table 3.

Table 3 The effect of the composition on the lifespan of Caenorhabditis elegans

Note: Compared with the solvent control group, *P<0.05, **P<0.01, ***P<0.001

It can be seen from Table 3 that both NMN and Rosa roxburghii extracts can prolong the lifespan of nematodes, but when NMN and Rosa roxburghii extracts are mixed according to a certain ratio, even if the doses of NMN and Rosa roxburghii extracts are reduced by half, the lifespan of nematodes can be extended The activities were significantly higher than the two alone.

In summary, the combination of NMN and Rosa roxburghii extract has synergistic effects, which can not only increase the abundance of beneficial bacteria, reduce the abundance of harmful bacteria, but also significantly increase the diversity index and abundance of intestinal microorganisms. It has an unexpected effect in improving the intestinal flora and delaying aging.

Claims (8)

1. The composition comprising β-nicotinamide mononucleotide and Rosa roxburghii extract is characterized in that its active ingredients are composed of the following components by weight percentage: β-nicotinamide mononucleotide is 45-65%, and the rest is thorn pear extract.
2. The composition comprising β-nicotinamide mononucleotide and Rosa roxburghii extract according to claim 1, wherein the active ingredient is composed of the following components by weight percentage: β-nicotinamide mononucleotide 50 ~60%, the rest is Rosa roxburghii extract.
3. The composition comprising β-nicotinamide mononucleotide and Rosa roxburghii extract according to claim 1, wherein the active ingredient is composed of the following components by weight percentage: β-nicotinamide mononucleotide 50 %, and the rest is Rosa roxburghii extract.
4. The composition comprising β-nicotinamide mononucleotide and Rosa roxburghii extract according to any one of claims 1 to 3, characterized in that: the Rosa roxburghii extract is a water extract.
5. The composition comprising β-nicotinamide mononucleotide and Rosa roxburghii extract according to any one of claims 1-3, characterized in that it also includes food acceptable auxiliary materials.
6. The composition comprising β-nicotinamide mononucleotide and Rosa roxburghii extract according to any one of claims 1 to 3, characterized in that: the composition comprising β-nicotinamide mononucleotide and Rosa roxburghii extract The composition is granule, tablet, capsule, pill, ointment, drink or biscuit.
7. Application of the composition comprising β-nicotinamide mononucleotide and Rosa roxburghii extract according to any one of claims 1 to 6 in the preparation of health products or medicines for improving intestinal flora disturbance.
8. Application of the composition comprising β-nicotinamide mononucleotide and Rosa roxburghii extract described in any one of claims 1 to 6 in the preparation of anti-aging health products or medicines.

Images