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WO2022220233A1 - Dispositif de mesure de gaz pour la mesure d'un gaz dans des organes digestifs de bétail - Google Patents

Dispositif de mesure de gaz pour la mesure d'un gaz dans des organes digestifs de bétail Download PDF

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Publication number
WO2022220233A1
WO2022220233A1 PCT/JP2022/017568 JP2022017568W WO2022220233A1 WO 2022220233 A1 WO2022220233 A1 WO 2022220233A1 JP 2022017568 W JP2022017568 W JP 2022017568W WO 2022220233 A1 WO2022220233 A1 WO 2022220233A1
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WO
WIPO (PCT)
Prior art keywords
gas
livestock
measuring
measuring device
insertion site
Prior art date
Application number
PCT/JP2022/017568
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English (en)
Japanese (ja)
Inventor
希恵 山田
洋治 乾
一洋 河合
共生 楜澤
Original Assignee
株式会社トクヤマ
学校法人麻布獣医学園
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 株式会社トクヤマ, 学校法人麻布獣医学園 filed Critical 株式会社トクヤマ
Priority to JP2023514650A priority Critical patent/JPWO2022220233A1/ja
Publication of WO2022220233A1 publication Critical patent/WO2022220233A1/fr

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/497—Physical analysis of biological material of gaseous biological material, e.g. breath

Definitions

  • the present invention relates to a new gas measuring device for measuring gas in the digestive organs of livestock.
  • Ruminant animals such as cows have four stomachs, and the plants they eat are first digested in the first stomach (rumen). At that time, hydrogen and carbon dioxide are generated in the process of decomposition (fermentation) of carbohydrates such as plants by bacteria present in the rumen. From this hydrogen and carbon dioxide, archaebacteria called methanogens produce methane gas. The produced methane gas is released into the atmosphere as a burp. Methane gas is said to have a greenhouse effect more than 25 times that of carbon dioxide, and 20 to 30% of methane gas in the atmosphere is said to be caused by the belching of ruminants.
  • Patent Literature 1 discloses a method of measuring exhalation with only the cow's head as a closed space.
  • Patent Literature 2 discloses a method of creating an artificial rumen model to simulate digestion in an actual cow.
  • Patent Literature 3 discloses a method of wirelessly measuring methane gas and the like by installing a device having a gas sensor in the stomach of a cow.
  • the measurement method disclosed in Patent Document 1 is only a method for measuring exhaled breath, and in addition to gas such as methane gas generated in the lumen, gas from exhaled breath is also measured. There was a problem that the gas could not be measured accurately.
  • the measurement method disclosed in Patent Literature 2 is a model-based simulation to the last, and cannot be said to be an accurate value.
  • the measurement method disclosed in Patent Document 3 is actually a method of making a cow swallow a measurement device with a gas sensor for measurement. There was a problem that it could not be measured accurately.
  • the rumen is in a state where a mixture of feed such as plants and digestive juices (hereinafter also referred to as "contents") exists during digestion.
  • a mixture of feed such as plants and digestive juices (hereinafter also referred to as "contents") exists during digestion.
  • the measuring device is buried in the space, there is also the problem that the generated gas cannot be measured accurately.
  • the measuring device is indwelled in the lumen, it can only be removed surgically. Therefore, in order to grasp the state of gas production such as methane gas, a method for accurately measuring gas such as methane gas produced by digestion of ruminants has been demanded.
  • the gas-measuring device has gas-detection means and gas-collection means
  • the gas collection means is composed of a livestock body insertion site A having a gas suction site and a livestock extracorporeal site B connected to the gas detection means.
  • a flow path is formed,
  • the livestock body insertion site A is composed of a flexible tube having a shape-retaining function;
  • a gas measurement device characterized by:
  • it is preferable that a reinforcing member for securing a shape retaining function is arranged in the tube, and further, the gas measuring device preferably has gas suction means.
  • a measuring method for measuring the gas in the livestock's digestive tract by inserting the livestock body insertion site A into the livestock's digestive tract using the gas measuring device described above.
  • the method of inserting the livestock body insertion site A into the digestive tract of livestock is not particularly limited, but examples thereof include a method of insertion through the oral cavity of livestock and a method of insertion through a fistula installed in the digestive tract of livestock.
  • the preferred method is insertion through a fistula.
  • gas measuring device of the present invention it is possible to accurately measure gases such as methane gas produced by digestion of ruminants, and it can be usefully utilized for research on means for reducing methane gas emissions from ruminants. .
  • FIG. 1 is a typical diagram of the gas measuring device of the present invention
  • FIG. 1 is a typical diagram of a gas measuring device provided with a diluting device of the present invention
  • FIG. 1 is a typical diagram of the gas measuring device of the present invention.
  • the gas detection means 1 is not particularly limited, and may be composed of a gas detector 11 corresponding to the type of gas to be measured. A plurality may be provided. In that case, a plurality of detectors can be arranged in series or in parallel, and in the case of parallel arrangement, a switching valve or the like may be provided.
  • the gas collection means 2 connected to the gas detection means 1 is composed of a livestock insertion site A having a gas suction point 22 and a livestock extracorporeal site B. is formed. It is essential that the livestock body insertion site A is composed of a flexible tube having a shape-retaining function.
  • the livestock extracorporeal site B is configured by a pipe that forms a gas flow passage, and various devices may be installed in this pipe. For example, in FIG. 1, a trap 31 for separating gases and a drying device 32 are provided.
  • FIG. 2 is a typical diagram of a gas measuring device provided with the dilution device of the present invention.
  • a dilution device may be provided at the livestock extracorporeal site B.
  • the diluting device is not particularly limited as long as it can dilute the gas to be measured, but in FIG. It's like
  • a flow meter 33 is provided in the flow path connected to the diluent gas supply pump 34, and a flow meter 33 is provided in the other flow path extending from the livestock body insertion site A (drying device 32). It is That is, these flow meters 33 can control the amount of diluent gas supplied by the diluent gas supply pump 34 (dilution concentration).
  • the gas may be sucked by natural suction due to the pressure of the gas generated in the digestive organs such as the lumen, but a suction pump may be used to stably suck the gas.
  • a suction pump may be used to stably suck the gas.
  • gas detector 11 is connected to suction pump 35 .
  • a pump-equipped gas detector 11 is also known, and naturally such a gas detector 11 can also be preferably used.
  • the gas collecting means 2 is composed of the livestock body insertion site A and the livestock extracorporeal site B, as described above.
  • the gas collection means 2 also has a gas flow function (that is, a gas flow path) from the gas suction point 22 (the tip of the insertion site A) to the gas detection means 1 .
  • the gas circulating function is literally a function of circulating the gas to be detected in the gas collecting means 2 .
  • the length of the livestock extracorporeal site B is not particularly limited, and is preferably in the range of 10 to 500 cm, more preferably in the range of 10 to 300 cm, and even more preferably in the range of 10 to 200 cm.
  • the livestock body insertion site A must be composed of a flexible tube that has a shape-retaining function. That is, the shape retention function means that when this tube (the tip of which is the gas suction point 22) is inserted into the digestive organs of livestock, the gas flow passage formed in the tube is not crushed by the pressure of the contents. It means that the tube shape is maintained to some extent, and "flexible” means that it has flexibility and is bendable to the extent that the tube can be inserted into the digestive organs of domestic animals.
  • the tube having the above characteristics is not particularly limited, but considering that it is inserted into digestive organs such as the lumen, it is made of a material with excellent chemical resistance, such as silicone resin or soft olefin resin. is preferred.
  • the inner diameter of such a tube is not particularly limited as long as it can collect gas in the body (inside the digestive organs), but is preferably in the range of 1 to 8 mm, more preferably in the range of 2 to 6 mm.
  • a reinforcing member 23 can be inserted into the tube in order to ensure the shape retention function described above.
  • the reinforcing member is, for example, a bundle of a plurality of linear members (so-called wires, etc.) twisted, twisted or bundled, or may be a bundle of thin hollow tubes.
  • FIG. 3 shows a reinforcing member in the form of twisted multiple wires.
  • the material may be plastic, and the number of linear members may be selected from 2 to 8 depending on the inner diameter of the tube. That is, by inserting such a reinforcing member 23 into the tube, even if a large external force is applied to the tube inside the livestock body, the tube can be prevented from being crushed. Not only is a space that serves as a gas flow path secured between the tube and the outer surface of the tube, but the flexibility of the tube is also secured.
  • the length of the above-described livestock body insertion site A is not particularly limited, but is preferably in the range of 10 to 40 cm, more preferably in the range of 10 to 30 cm.
  • the insertion site A when the insertion site A is inserted into the gastrointestinal tract of livestock, particularly into the rumen of ruminants such as cattle, and gas is sucked, the insertion site A is inserted for the purpose of preventing the contents of the rumen from being aspirated.
  • a filter may be provided at the tip portion.
  • a float may be provided at the tip of the insertion site A for the purpose of preventing the tip of the insertion site A from being buried in the contents (such as undigested matter) existing in the livestock body.
  • a part of the livestock extracorporeal site B that is continuous with the livestock body insertion site A is also composed of a flexible tube having a shape-retaining function. It is preferable to consider the manipulation of the insertion site A in the body.
  • the length of the flexible tube portion having a shape-retaining function at the livestock extracorporeal site B is preferably in the range of 5 to 30 cm, more preferably in the range of 10 to 20 cm.
  • the gas detected in the present invention is not particularly limited, and examples include methane gas, carbon dioxide, oxygen, carbon monoxide, ammonia, volatile fatty acids, and the like.
  • gas in the gastrointestinal tract of livestock is measured using the gas measuring device described above. That is, the gas suction point 22 of the livestock body insertion site A is inserted into the rumen of the livestock's digestive organ, and the gas inside the digestive organ, particularly the rumen, is measured.
  • the insertion of the insertion site A into the digestive tract is not particularly limited, and it may be inserted through the oral cavity of livestock, but it is preferable to use a fistula in order to reliably aspirate the gas in the digestive tract. be.
  • a fistula 52 is installed in the lumen 51, and the Preferably, site A is inserted within lumen 51 .
  • the insertion site A (tube) is deformed as necessary so as to avoid the contents 53 in the lumen 51, and then the fistula 52 is inserted into the void space in the upper part of the lumen 51 where gas exists.
  • the insertion site A is inserted via By passing through the fistula 52, the position of the void in the upper part of the lumen 51 can be easily grasped, so that the tip of the insertion site A (gas suction point 22) can be positioned more accurately.
  • the lid of the fistula 52 is provided with a minimum hole through which the insertion site A can be passed, and the base of the insertion site A ( It is preferable that the end portion on the side of the extracorporeal site B) is fitted and fixed.
  • the content 53 increases and decreases during the process of digestion and feeding, and the void space in the upper part of the rumen 51 also increases and decreases. In that case, the position of the gas suction point 22 at the tip of the livestock body insertion site A may be adjusted appropriately.

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Food Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Sampling And Sample Adjustment (AREA)

Abstract

Le présent dispositif de mesure de gaz permettant de mesurer un gaz dans des organes digestifs de bétail comprend un moyen de détection de gaz (1) et un moyen de collecte de gaz (2), et est caractérisé en ce que : le moyen de collecte de gaz (2) est constitué d'une partie insertion dans le corps du bétail (A) doté d'un emplacement d'aspiration de gaz (22), et d'une partie hors du corps du bétail (B) dans le prolongement du moyen de collecte de gaz (2) ; un passage d'écoulement de gaz est formé depuis l'emplacement d'aspiration de gaz (22) jusqu'au moyen de détection de gaz (1) ; et la partie insertion dans le corps du bétail (A) est conçue à l'aide d'un tube flexible présentant une fonction de retenue de forme.
PCT/JP2022/017568 2021-04-16 2022-04-12 Dispositif de mesure de gaz pour la mesure d'un gaz dans des organes digestifs de bétail WO2022220233A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2023514650A JPWO2022220233A1 (fr) 2021-04-16 2022-04-12

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2021069897 2021-04-16
JP2021-069897 2021-04-16

Publications (1)

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WO2022220233A1 true WO2022220233A1 (fr) 2022-10-20

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WO (1) WO2022220233A1 (fr)

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08510948A (ja) * 1994-01-11 1996-11-19 フィリップス，マイケル 呼気収集装置
JP2005098817A (ja) * 2003-09-24 2005-04-14 Toshiba Plant Systems & Services Corp 家畜の呼気成分測定方法、測定装置および記録媒体
JP2007175149A (ja) * 2005-12-27 2007-07-12 Abilit Corp 呼気収集装置
JP2014525738A (ja) * 2011-07-01 2014-10-02 コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガニゼイション 哺乳動物の胃の中のガスを測定するためのシステム、方法、及び装置
KR20170036933A (ko) * 2015-09-24 2017-04-04 대구대학교 산학협력단 소의 반추위에서 생성되는 메탄가스 실시간 측정장치 및 방법

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08510948A (ja) * 1994-01-11 1996-11-19 フィリップス，マイケル 呼気収集装置
JP2005098817A (ja) * 2003-09-24 2005-04-14 Toshiba Plant Systems & Services Corp 家畜の呼気成分測定方法、測定装置および記録媒体
JP2007175149A (ja) * 2005-12-27 2007-07-12 Abilit Corp 呼気収集装置
JP2014525738A (ja) * 2011-07-01 2014-10-02 コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガニゼイション 哺乳動物の胃の中のガスを測定するためのシステム、方法、及び装置
KR20170036933A (ko) * 2015-09-24 2017-04-04 대구대학교 산학협력단 소의 반추위에서 생성되는 메탄가스 실시간 측정장치 및 방법

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
"Developing a simplified measurement technology for methane gas emitted by cattle (Argentina)", ALIC LIVESTOCK OVERSEAS INFORMATION, AGRICULTURE & LIVESTOCK INDUSTRIES CORPORATION, JAPAN, Japan, pages 1 - 2, XP009540832, Retrieved from the Internet <URL:https://lin.alic.go.jp/alic/week/2008/ar/ar20080822.htm> *

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