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WO2022021377A1 - Pipette et procédé de pipetage - Google Patents

Pipette et procédé de pipetage Download PDF

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Publication number
WO2022021377A1
WO2022021377A1 PCT/CN2020/106331 CN2020106331W WO2022021377A1 WO 2022021377 A1 WO2022021377 A1 WO 2022021377A1 CN 2020106331 W CN2020106331 W CN 2020106331W WO 2022021377 A1 WO2022021377 A1 WO 2022021377A1
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WO
WIPO (PCT)
Prior art keywords
pipette
injection
plunger
inner diameter
slider
Prior art date
Application number
PCT/CN2020/106331
Other languages
English (en)
Chinese (zh)
Inventor
刘新志
苑宝龙
Original Assignee
杭州九洋生物科技有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 杭州九洋生物科技有限公司 filed Critical 杭州九洋生物科技有限公司
Priority to PCT/CN2020/106331 priority Critical patent/WO2022021377A1/fr
Publication of WO2022021377A1 publication Critical patent/WO2022021377A1/fr

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes

Definitions

  • the present invention relates to the technical field of pipettes, in particular to a pipette and a pipetting method.
  • the pipette of the present invention is the core technology unit in the biological sample automatic processing system, and can be applied to a pipetting workstation composed of a combination of multi-channel electric pipettes.
  • the gas-liquid mixing displacement pipette is composed of an electric industrial syringe pump, a syringe, a reversing valve, a voltage regulator, a catheter, a pipetting head, a sensor, and a pipetting actuator.
  • the rear end of the catheter is connected to the syringe of the electric industrial syringe pump through a voltage regulator, a reversing valve, a pressure sensor, etc., and the syringe and the catheter are filled with liquid by the action of the electric industrial syringe pump, forming a liquid column with the function of a syringe plunger
  • the stopper, the leading end of the catheter and its components form a docking with the exchangeable pipette tip, leaving only an air gap in the pipette tip, isolating the liquid plunger and the sample liquid.
  • the advantage of this method is that the electric reciprocating motion injection mechanism and the pressure sensor are separated, and only one thin tube enters the pipetting actuator, which greatly reduces the design pressure and load of the pipetting actuator assembly and meets the basic center distance of the target plate and vessel. Require. At the same time, the distance between the reciprocating motion axis of the pipetting actuator and the vertical line of the fulcrum of the mechanism can be minimized, and the harmful overturning moment and support generated when the pipetting actuator is frequently pressed down with the pipetting head along its axis downwards is reduced. Part fatigue deformation. And because the isolation air gap between the liquid plunger and the sample liquid is small, the pipetting accuracy is high.
  • the disadvantage is that there is a gas-liquid pipeline pressure system, many components, complicated debugging and high maintenance costs. And when the room temperature is low, it is easy to leak liquid.
  • the gas displacement pipette is composed of a motor, a syringe made of metal or other materials, a sensor, and a pipetting actuator.
  • the reciprocating motion of the solid material plunger is carried out by the motor through the coupling or other connecting parts to drive the screw or timing belt. That is to say, the components such as the electric reciprocating injection mechanism and the pressure sensor are assembled with the pipetting actuator, and there is no need for complicated pressure pipelines and pump systems.
  • the isolation air gap between the solid plunger and the sample liquid depends on the result of structural design concessions. The smaller the isolation air gap, the smaller the pipetting uncertainty range, and vice versa.
  • the present invention provides a pipette, which belongs to a gas displacement type pipette, and the pipette can not only satisfy the center distance between the motion axes of adjacent injection chambers as the basic center distance of the target plate vessel, It will not waste the utilization rate of the injection cavity. It can not only be applied to conventional target plate utensils, but also can be applied to ultra-small target plate utensils, which greatly improves the preparation efficiency of target plate samples, and has high economical practicability and feasibility. Extensibility, thereby at least partially solving the above-mentioned defects and deficiencies in the prior art.
  • the present invention provides a pipette.
  • the pipette includes a housing, a driving device, 6 injection parts and a transmission device.
  • the housing has an inner cavity.
  • the driving device is provided outside the casing.
  • the injection parts are arranged through the housing, wherein the 6 injection parts are arranged in a 2 ⁇ 3 matrix.
  • At least a part of the transmission device is provided in the inner cavity, the transmission device is connected to each of the 6 injection parts and the driving device, so that the 6 injection parts are sucked synchronously. liquid or drain.
  • each of the injection components includes a syringe, a plunger, a seal, and a pipette tip.
  • the injection tube is disposed through the housing, an injection cavity is formed inside the injection tube, and the lower end of the injection tube has a convex and concave portion.
  • the plunger is arranged in the injection cavity, and the upper end of the plunger is connected with the transmission device so as to be able to move at least between a maximum liquid suction position and a maximum liquid discharge position.
  • the seal is provided outside the plunger and on the upper part of the injection cavity for sealing the injection cavity.
  • the pipette tip is fitted at the convex and concave portion through an interference fit.
  • the driving device includes a motor, the motor has a shaft extension, and the transmission device includes a motion pair, a screw pair and a slider.
  • the motion pair is connected with the housing, and the motion pair includes a driving synchronous pulley, a synchronous belt and a follow-up synchronous pulley, wherein the driving synchronous pulley is fixedly connected to the shaft extension.
  • the screw pair is arranged in the inner cavity, and the screw pair includes a screw rod that is fixedly connected with the follow-up synchronous pulley and an anti-backlash nut that can move up and down along the screw rod.
  • the sliding block is arranged in the inner cavity, the sliding block is fixedly connected with the anti-backlash nut and can slide up and down along the screw rod, and the lower end of the sliding block is connected to the upper end of the plunger.
  • the plunger includes a plunger body and a step portion disposed on the upper end of the plunger body, and the lower end of the slider is provided with a notch, the notch includes a first inner diameter portion and a notch located at the upper end of the plunger body.
  • an adjusting top wire and a first elastic member are arranged in the first inner diameter portion, the first elastic member abuts the stepped portion above the stepped portion, and the upper end of the adjusting top wire is connected to the above-mentioned stepped portion.
  • the sliding block is connected, and the lower end of the adjusting top wire abuts the upper end of the first elastic member.
  • the transmission device further includes a guide rail pair, and the guide rail pair includes an optical axis and a linear bearing.
  • the optical axis is fixedly arranged in the inner cavity.
  • the linear bearing is arranged outside the optical axis and can slide up and down along the optical axis, and the linear bearing is also arranged in the slider. The slider can slide along the guide rail pair.
  • the pipette further includes a positioning mechanism, and the positioning mechanism includes a positioning sheet and a reference position sensor.
  • the positioning piece is arranged on the slider.
  • the reference position sensor is provided on the casing.
  • the pipette further includes a pipette head withdrawal mechanism, and the pipette head withdrawal mechanism includes a top rod, a sliding pressure block and a second elastic member.
  • the top rod is vertically arranged in the inner cavity and can slide vertically relative to the housing.
  • the sliding pressing block is arranged outside the injection tube and can slide along the injection tube, and the sliding pressing block is located under the ejector rod.
  • the second elastic member is disposed outside the top rod, the upper end of the second elastic member is connected to the housing, and the lower end of the second elastic member is connected to the sliding pressure block.
  • the center-to-center distance between two adjacent injection parts is 9 mm.
  • the present invention also provides a pipetting method using any of the above-mentioned pipettes.
  • the pipette and the pipetting method of the present invention by setting a reasonable working mode, the pipette can be configured on pipetting workstations for different purposes, not only can be applied to conventional target plate utensils, but also can be applied to ultra-small Target plate utensils, thereby greatly improving the preparation efficiency of target plate samples without wasting the utilization rate of the injection cavity.
  • FIG. 1 is a front view of a pipette according to an embodiment of the present invention, wherein the cover plate of the housing is removed in order to clearly show the internal structure of the pipette;
  • Figure 2 is a cross-sectional view of the pipette shown in Figure 1 along line A-A in Figure 1;
  • Figure 3 is a schematic bottom view of the pipette shown in Figure 1;
  • Fig. 4 is the process schematic diagram of using the pipette according to the present invention to carry out the pipetting operation on the target plate vessel one;
  • Fig. 5 is the process schematic diagram of using the pipette according to the present invention to carry out the pipetting operation on the target plate vessel two;
  • FIG. 6 is a schematic diagram of the process of pipetting on the target plate vessel three using the pipette according to the present invention.
  • FIG. 1 is a front view of a pipette according to an embodiment of the present invention, wherein the cover plate of the housing is removed in order to clearly show the internal structure of the pipette;
  • FIG. 2 is shown in FIG. 1 A cross-sectional view of the pipette along the line AA in FIG. 1;
  • FIG. 3 is a schematic bottom view of the pipette shown in FIG. 1;
  • 5 is a schematic diagram of the process of using the pipette according to the present invention to carry out the pipetting operation on the target plate vessel two;
  • FIG. 6 is the use of the pipette according to the present invention. Schematic diagram of the operation process.
  • the pipette 100 according to an embodiment of the present invention will be described in detail below with reference to FIGS. 1 to 6 .
  • the illustrated pipette 100 includes a housing 110 , a driving device 120 provided outside the housing 110 , six injection parts 130 provided through the housing 110 , and a transmission device 140 .
  • the injection part 130 and the transmission device 140 constitute the actuator of the pipette 100 .
  • the housing 110 has an inner cavity 115 , and the inner cavity 115 can form an accommodation space for accommodating at least a part of the transmission device 140 of the pipette 100 and components such as the injection part 130 .
  • the housing 110 includes an upper end block 2 , a lower end block 13 , and a frame 6 disposed between the upper end block 2 and the lower end block 13 .
  • the upper end block 2 and the lower end block 13 can be connected to the frame 6 by welding connection or threaded fastener connection, and together with the frame 6 form an inner cavity 115 having an opening on the front side.
  • the housing 110 further includes a cover plate 24 covering the front side opening of the inner cavity 115, and the cover plate 24 can be detachably connected to the upper end block 2 and the lower end block 3 by means of threaded fasteners, so as to facilitate pipetting
  • the cover plate 24 can be detachably connected to the upper end block 2 and the lower end block 3 by means of threaded fasteners, so as to facilitate pipetting
  • At least a part of the transmission 140 of the device 100 , the injection part 130 and other components are arranged in the inner cavity 115 .
  • the housing 110 may be made of a higher strength metal material (eg, aluminum, copper, steel, etc.) in order to provide the mechanical strength required to support various components inside the housing 110 .
  • a higher strength metal material eg, aluminum, copper, steel, etc.
  • the housing 110 is made of aluminum alloy with lower density.
  • the driving device 120 is disposed outside the casing and is fixedly connected with the casing 110 . Specifically, in this embodiment, the driving device 120 is fixedly connected to the upper end block 2 of the housing 110 .
  • the drive device 120 can provide energy to the injection part 130 during operation of the pipette 100 .
  • the pipette 110 further includes six injection parts 130 , and the injection parts 130 are disposed through the housing 110 .
  • the injection part 130 is vertically disposed through the lower end block 13 of the housing 110 . Liquid can be drawn into the injection part 130 or discharged from the injection part 130 .
  • the six injection parts 130 are arranged in a 2 ⁇ 3 matrix.
  • the pipette 100 of the present invention can be configured on pipetting workstations for different purposes, not only can be applied to conventional target plate utensils, but also can be applied to ultra-small target plate Therefore, the preparation efficiency of the target plate sample is greatly improved without wasting the utilization rate of the injection cavity.
  • the operation process of pipetting using the pipette 100 of the present invention will be described in more detail later with reference to FIGS. 4 to 6 .
  • the transmission device 140 is connected to each of the 6 injection parts 130 and the driving device 120 so that the 6 injection parts 130 perform aspiration or discharge synchronously.
  • the transmission 140 is disposed in the inner cavity 115 of the housing 110 so as to be connected to each of the six injection members 130 in the inner cavity 115 of the housing 110, and
  • the transmission device 140 is connected with the driving device 120 , so that the driving device 120 can drive the six injection parts 130 through the transmission device 140 to perform liquid suction or discharge synchronously, which ensures the consistency of the movements of the six injection parts 130 .
  • each of the injection components 130 includes a syringe 14 , a plunger 17 , a seal 26 , and a pipetting head 16 .
  • the injection tube 14 is arranged through the housing 110 and is fixedly arranged relative to the housing 110 .
  • An injection cavity 131 is formed inside the injection tube 14, and the lower end of the injection tube 14 has a convex and concave portion.
  • the injection tube 14 is provided through the lower end block 13 of the housing 110 .
  • the plunger 17 is arranged in the injection cavity 131, and the upper end of the plunger 17 is connected with the transmission device 140 so as to be able to move at least between the maximum liquid suction position and the maximum liquid discharge position.
  • the “maximum suction position” referred to here refers to the position where the maximum volume is suctioned in the injection part 130 (specifically, the pipette head 16 with the maximum volume or volume of the injection part 130 which will be described in detail below).
  • the position of the plunger 17 when the liquid is located is the position where the lower end of the plunger 17 is located at the uppermost end of the injection cavity 131 .
  • the “maximum discharge position” refers to the position at which the plunger 17 is located when the liquid is completely discharged from the injection part 130 .
  • the sealing member 26 is provided outside the plunger 17 and on the upper part of the injection cavity 131 for sealing the injection cavity 131 .
  • the pipetting head 16 is assembled at the convex and concave portion of the lower end of the syringe 14 by interference fit, so as to realize static sealing. Wherein, the pipette heads 16 with different ranges can be assembled at the convex and concave portion of the lower end of the injection tube 14 according to requirements.
  • the pipette tip 16 may be a pipette tip with a standardized volume of 10 microliters to 1000 microliters.
  • the pipetting head 16 is a disposable item, usually made of plastic, which is a consumable item and is discarded after one liquid treatment is completed.
  • the syringe 14 can be inserted into the pipette head 16 by moving axially downward together with the housing 100 , so that the disposable pipette heads 16 of different ranges can be inserted with the convex and concave structure of the syringe 14 .
  • the pipette head 16 is immersed in the liquid to be suctioned, the plunger 17 is driven by the transmission device 140 to move upward toward the maximum suction position, and the liquid is sucked into the pipette head 16; during the discharge process, the plunger 17 Driven by the transmission device 140 to move downward toward the maximum discharge position, the liquid is discharged from the pipetting head 16 . It should be noted that, during the liquid suction or discharge process, the liquid does not enter the injection cavity 131, but only stays in the pipetting head 16, thereby avoiding cross-contamination of the liquid.
  • the size of the range of each liquid treatment depends on the volume of the pipetting head 16 used, and the size of the range of liquid treatment depends on the volume of the plunger 17 or the maximum suction position of the plunger 17, wherein,
  • the maximum pipetting position of the plunger 17 can be configured such that during pipetting, the liquid just fills the pipetting head 16 of maximum volume or volume without entering the injection chamber 131 .
  • the injection part 130 further includes a sealing adjustment nut 12 to press the sealing member 26 against the upper part of the injection cavity 131 .
  • the sealing member 26 is an O-shaped sealing ring, and is sandwiched between the injection tube 14 and the sealing adjustment nut 12.
  • the O-shaped sealing ring 26 and the sealing adjustment nut 12 can seal the injection tube 14 and the column.
  • the plug 17 is connected in a sliding and sealing manner to achieve sliding sealing.
  • the transmission device 140 drives the plunger 17 to move upward along the axial direction of the plunger 17, so that the liquid is sucked into the pipette head 16, and during the liquid discharge process, the transmission device 140 drives the plunger 17 along the plunger The axial direction of 17 moves downwards so that the liquid is expelled from the pipetting head 16 .
  • the driving device 120 includes a motor 1 having a shaft extension 121
  • the transmission device 140 includes a motion pair 141 , a screw pair 142 and a slider 10 .
  • the motion pair 141 is connected to the housing 110 , and the motion pair 141 includes a driving synchronous pulley 3 , a synchronous belt 4 and a follow-up synchronous pulley 5 , wherein the driving synchronous pulley 3 is connected to the motor 1 .
  • Shaft extension 121 As shown in FIG. 1 to FIG. 2 , the motion pair 141 is connected to the housing 110 , and the motion pair 141 includes a driving synchronous pulley 3 , a synchronous belt 4 and a follow-up synchronous pulley 5 , wherein the driving synchronous pulley 3 is connected to the motor 1 .
  • Shaft extension 121 is provided to the housing 110 , and the motion pair 141 includes a driving synchronous pulley 3 , a synchronous belt 4 and a follow-up synchronous pulley 5 , wherein the driving synchronous pulley 3 is connected to the motor 1 .
  • the motion pair 141 is connected to the upper end block 2 of the housing 110, and the driving synchronous pulley 3 is fixedly connected to the shaft extension 121 of the motor 1, so as to be able to move together with the shaft extension 121 of the motor 1, and
  • the inner teeth of the synchronous belt 4 can mesh with the outer teeth of the driving synchronous pulley 3 and the follower synchronous pulley 5, so that when the shaft extension 121 of the motor 1 drives the driving synchronous pulley 3 to rotate, the driving synchronous pulley 3 can pass through the synchronization.
  • the belt 4 drives the follow-up synchronous pulley 5 to rotate together.
  • the motor 1 is further provided with an encoder (not shown), so as to control the rotational speed, forward and reverse rotation and other motion parameters of the motor 1 .
  • the screw pair 142 is arranged in the inner cavity 115 , and the screw pair 142 includes a screw 8 fixedly connected with the follower synchronous pulley 5 and an anti-backlash nut 20 that can move up and down along the screw 8 .
  • the anti-backlash nut 20 is threadedly connected with the screw rod 8 , so that the anti-backlash nut 20 can move upward or downward in the axial direction of the screw rod 8 .
  • the screw pair 142 further includes an upper bearing 23 and a lower bearing 25 arranged outside the screw 8, wherein the upper bearing 23 is also fixedly arranged in the upper end block 2 of the housing 110, and the lower bearing 25 is fixedly arranged in the housing 110 in the lower end block 13.
  • the upper bearing 23 , the lower bearing 25 and the screw rod 8 are coaxially arranged, so that the screw rod 8 maintains a vertical state during the rotation process, thereby driving the anti-backlash nut 20 to move axially upward or downward.
  • the sliding block 10 is arranged in the inner cavity 115 , the sliding block 10 is fixedly connected with the anti-backlash nut 20 and can slide up and down along the screw 8 , and the lower end of the sliding block 10 is connected to the upper end of the plunger 17 .
  • the screw 8 can drive the anti-backlash nut 20 to move axially upward or downward
  • the anti-backlash nut 20 is fixedly connected with the slider 10
  • the lower end of the slider 10 is connected to the upper end of the plunger 17, it can be seen that,
  • the follow-up synchronous pulley 5 drives the screw 8 to rotate, the slider 10 can slide up and down along the screw 8 to drive the plunger 17 to move up and down, and then perform liquid suction or discharge.
  • the plunger 17 includes a plunger body and a stepped portion 171 provided at an upper end of the plunger body.
  • the stepped portion 171 is connected to the plunger body and protrudes from the plunger body.
  • the lower end of the slider 10 is provided with a slot 28 .
  • the slot 28 includes a first inner diameter portion and a second inner diameter portion located at a lower end of the first inner diameter portion. The inner diameter of the first inner diameter portion is greater than or equal to the outer diameter of the stepped portion 171 of the plunger 17 .
  • the inner diameter of the second inner diameter portion is smaller than the inner diameter of the first inner diameter portion to form an abutment surface in the notch 28 , and the inner diameter of the second inner diameter portion is equal to the outer diameter of the plunger body, and the stepped portion 171 of the plunger 17 can abut against The upper part of the resting surface is fitted with the resting surface. In this way, the plunger 17 will not have any displacement in the horizontal direction relative to the slider 10, which is beneficial to the accuracy of the injection part 130 during the pipetting process.
  • the abutment surface in the slot 28A is perpendicular to the longitudinal axis of the plunger 17 .
  • a first elastic member 18 and an adjusting top wire 19 are also arranged in the first inner diameter portion of the slot 28 of the slider 10 .
  • the first elastic member 18 is disposed above the stepped portion 171 of the plunger 17 and abuts against the stepped portion 171 of the plunger 17 .
  • the adjusting top wire 19 is arranged above the first elastic member 18 .
  • the upper end of the adjusting top wire 19 is connected to the slider 10 , and the lower end of the adjusting top wire 19 abuts against the upper end of the first elastic member 18 .
  • the first elastic member 18 In the working state of the pipette 100, the first elastic member 18 is in a compressed state or a natural extension state. In this way, the plunger 17 does not have any displacement in the vertical direction relative to the slider 10 .
  • the transmission device 140 further includes a guide rail pair 145 , and the guide rail pair 145 includes the optical axis 7 and a linear bearing (not shown).
  • the optical axis 7 is fixedly arranged in the inner cavity 115 .
  • the guide rail pair 145 includes two optical axes 7, which are arranged side by side at intervals on both sides of the screw 8 and are fixedly arranged between the upper end block 2 and the lower end block 13 through the slider 10 During this time, the longitudinal axis of the optical axis 7 is parallel to the longitudinal axis of the screw 8 .
  • the linear bearing is arranged outside the optical axis 7 and can slide up and down along the optical axis 7 , and the linear bearing is also arranged in the slider 10 , wherein the slider 10 can slide along the guide rail pair 145 .
  • the optical axis 7 is used as the slide rail of the slider 10 to guide the slider 10 to slide up and down along the optical axis 7, so that when the driving device 120 drives the screw 8 to rotate, the anti-backlash nut 20 drives the slider 10 to slide along the optical axis 7.
  • the optical axis 7 slides up and down, that is, the slider 10 can slide along the guide rail pair 145 .
  • the pipette 100 further includes a positioning mechanism 150.
  • the positioning mechanism 150 includes a positioning piece 21 and a reference position sensor 22.
  • the positioning piece 21 is provided on the slider 10, and the reference position sensor 22 is provided on the housing. 110 on.
  • the positioning piece 21 is arranged on the lower part of the slider 10
  • the reference position sensor 22 is arranged on the upper surface of the lower end block 13 of the housing 110 , and the positioning piece 21 and the reference position sensor 22 are vertically opposite to each other. so that when the positioning piece 21 moves downward with the slider 10, the reference position sensor 22 can detect the position of the slider 10 by detecting the position of the positioning piece 21, and then the pipetting head 16 can be judged by the position of the slider 10.
  • the slider 10 drives the plunger 17 to move downward.
  • the positioning piece 21 on the slider 10 detects the initial position signal, it stops running and records this position as the initial position (reset).
  • the reference position sensor 22 can also be connected to the encoder of the motor 1 to feed back the real-time position of the slider 10 to the encoder.
  • the reference position sensor 22 may be a photoelectric sensor.
  • the pipette 100 further includes a pipette head withdrawal mechanism 160
  • the pipette head withdrawal mechanism 160 includes a top rod 11 , a sliding pressing block 15 and a second elastic member 27 .
  • the ejector rod 11 is vertically disposed in the inner cavity 115 and can slide vertically relative to the housing 110 .
  • the sliding pressure block 15 is disposed outside the injection tube 14 and can slide along the injection pipe 14 , and the sliding pressure block 15 is located below the ejector rod 11 .
  • the second elastic member 27 is disposed outside the top rod 11 , the upper end of the second elastic member 27 is connected to the housing 110 , and the lower end of the second elastic member 27 is connected to the sliding pressure block 15 .
  • the top rod 11 is vertically disposed in the inner cavity 115 through the lower end block 13 of the housing 110 , the top rod 11 is located under the slider 10 and is fixedly connected to the sliding pressure block 15 .
  • the second elastic member 27 is in a natural extension state.
  • the slider 10 can go down by a few millimeters.
  • the slider 10 moves down and pushes the ejector rod 11 and the sliding pressure block 15.
  • the upper portion of the pipetting head 16 disposed outside the syringe 14 applies pressure so that the pipetting head 16 falls from the syringe 14 .
  • the sliding pressing block 15 is reset under the elastic force of the second elastic member 27 .
  • the sliding pressure block 15 of the pipette head exit mechanism 160 is directly sleeved outside the injection tube 14 , and the ejector rod 11 and the sliding pressure block 15 are driven by the slider 10 to move downward, that is, The pipette head 16 can be withdrawn, and the driving device 120 of the plunger 17 is directly used as the driving device of the sliding pressure block 15.
  • the structure is simple and compact, and no complicated pipette head withdrawal mechanism and driving electromagnetic components are required, thereby reducing the cost.
  • the center-to-center distance between two adjacent injection parts 130 is 9 mm.
  • the six injection parts 130 are arranged in a 2 ⁇ 3 matrix, so that the pipette 100 of the present invention can be configured on the pipetting workstations of different purposes under the automatic structure.
  • the target plate utensils with a distance of 9mm can also be applied to various target plate utensils with a center distance of 4.5mm and 2.25mm, and in the process of sample preparation, the channel utilization rate is not wasted.
  • the working modes of the pipette 100 according to the present invention on target plates of different sizes are described.
  • Figures 4 to 6 show target plate vessels that have been standardized on the market today.
  • the target plate vessel 200 is shown in FIG. 4 , wherein the target plate vessel 200 includes 96 accommodating parts 201 for accommodating samples, and the 96 accommodating parts 201 are arranged and distributed in an 8 ⁇ 12 matrix, and the The center-to-center distance between two adjacent accommodating portions 201 is 9 mm.
  • the pipette 100 first completes the preparation of the sample on the six accommodating parts 201 located at the position 0, and then moves the injection part 130 downward, so that the injection part 130 is located at the position 0.
  • Above the 6 accommodating parts 201 at site 1 and complete the preparation of the sample similarly, make the injection parts 130 respectively located above the sites 2, 3, . . .
  • samples prepared by the component 130 are evenly distributed on each accommodating portion 201 of the target plate vessel 200 , and are only distributed in the accommodating portion of the target plate vessel 200 , not outside the target plate vessel 200 .
  • samples can be prepared in 6 accommodating parts 201 at the same time, which significantly improves the efficiency of sample preparation; on the other hand, the prepared samples can be evenly distributed in the in the accommodating portion 201 , and will not fall on other areas outside the target plate vessel 200 .
  • the target plate vessel 300 is shown in FIG. 5 , wherein the target plate vessel 300 includes 96 accommodating parts 301 for accommodating samples, and the 96 accommodating parts 301 are arranged and distributed in a matrix of 8 ⁇ 12, and the The center-to-center distance between two adjacent accommodating portions 301 is 4.5 mm.
  • the pipette 100 first completes the preparation of the sample on the 6 receptacles 301 located at the site 0, and then moves the injection part 130 to the 6 receptacles 301 located at the site 1. Above the accommodating parts 301 and complete the preparation of the sample, similarly, the injection part 130 is located above the sites 2, 3, .
  • the target plate vessel 400 is shown in FIG. 6 , wherein the target plate vessel 400 includes 96 accommodating portions 401 for accommodating samples, and the 96 accommodating portions 401 are arranged and distributed in an 8 ⁇ 12 matrix, and the The center-to-center distance between two adjacent accommodating portions 401 is 2.25 mm.
  • the pipette 100 first completes the preparation of the sample on the 6 receptacles 401 located at the site 0, and then moves the injection part 130 to the 6 receptacles 401 located at the site 1. Above the accommodating parts 401 and complete the preparation of the sample, similarly, the injection part 130 is located above the positions 2, 3, .
  • pipette 100 of the present invention is also applicable to any number of stacked target plates, such as two, three or more target plates.
  • the pipette 100 of the present invention can be configured on pipetting workstations for different purposes, and can be applied not only to conventional target plate vessels, but also to ultra-small target plate vessels, so that injections are not wasted.
  • the preparation efficiency of the target plate sample is greatly improved, and it has high economical practicability and scalability.
  • the motor 1 When the liquid transfer starts, the motor 1 is put into operation in a timely manner, and the drive synchronous pulley 3 drives the follow-up synchronous pulley 5 to rotate through the synchronous belt 4, thereby driving the screw 8 to rotate, and the screw 8 drives the slider 10 along the optical axis through the anti-backlash nut 20.
  • the slider 10 can drive the plunger 17 to move up and down in the injection tube 14, so as to perform liquid suction or discharge.
  • the plunger 17 moves upward, and the liquid is sucked into the pipetting head 16 , and when the plunger 17 moves downward, the liquid is discharged from the pipetting head 16 .
  • the liquid does not enter the injection cavity 131, but only stays in the pipetting head 16, thereby avoiding cross-contamination of the liquid.
  • a target plate vessel 300 is used to prepare a sample.
  • the target plate vessel 300 includes 96 accommodating portions for accommodating samples, and the 96 accommodating portions 301 are arranged and distributed in an 8 ⁇ 12 matrix. And the center-to-center distance between two adjacent accommodating portions 301 is 4.5 mm.
  • the pipette 100 first completes the preparation of the sample on the 6 receptacles 301 located at the site 0, and then moves the injection part 130 to the 6 receptacles 301 located at the site 1.
  • the injection part 130 is located above the sites 2, 3, .
  • Distributed on each accommodating portion 301 of the target plate vessel 300 and only distributed in the accommodating portion of the target plate vessel 300 , and not distributed outside the target plate vessel 300 .

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  • Chemical & Material Sciences (AREA)
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  • Sampling And Sample Adjustment (AREA)

Abstract

L'invention concerne une pipette (100) et un procédé de pipetage. La pipette (100) comprend un boîtier (110), un dispositif d'entraînement (120), six constituants d'injection (130) et un dispositif de transmission (140). Le boîtier (110) présente une cavité interne (115), et le dispositif d'entraînement (120) est disposé à l'extérieur du boîtier. Les constituants d'injection (130) sont disposés à travers le boîtier (110), les six constituants d'injection (130) étant disposés selon une forme matricielle de 2×3. Au moins une partie du dispositif de transmission (140) est disposée dans la cavité interne (115). Le dispositif de transmission (140) est relié à chacun des six constituants d'injection (130) et au dispositif d'entraînement (120), de telle sorte que les six constituants d'injection (130) prennent de manière synchrone un liquide ou un liquide de décharge. Selon la pipette (100) et le procédé de pipetage, par réglage d'un mode de fonctionnement raisonnable, la pipette (100) peut être disposée sur des postes de travail de pipetage ayant différentes utilisations, et la pipette (100) est non seulement applicable à des récipients de plaques cibles classiques, mais également applicable à des récipients à plaque cible ultra-petites, de telle sorte que l'efficacité de préparation pour des échantillons de plaque cibles est considérablement améliorée sans abaisser le taux d'utilisation d'une cavité d'injection.
PCT/CN2020/106331 2020-07-31 2020-07-31 Pipette et procédé de pipetage WO2022021377A1 (fr)

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CN115055217A (zh) * 2022-07-04 2022-09-16 广州微远医疗器械有限公司 一种用于移液泵脱吸头的装置
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