WO2019078070A1

WO2019078070A1 - Laminate

Info

Publication number: WO2019078070A1
Application number: PCT/JP2018/037824
Authority: WO
Inventors: 裕介塚村; 大井　亮; 川端　裕介
Original assignee: 東レ株式会社
Priority date: 2017-10-20
Filing date: 2018-10-10
Publication date: 2019-04-25
Also published as: JPWO2019078070A1; TW201922472A

Abstract

In view of this prior art background, the present invention provides a laminate that is suitable for a biosensor which reduces the amount of use of a liquid sample, while having a simplified production process. A laminate which sequentially comprises a cover material B, a resin layer X, a base material A, a resin layer Y and a cover material C in this order, and which is characterized in that: the laminate internally has a cavity that is provided with a feed port; and the cavity is configured from a specific surface.

Description

Laminate

The present invention relates to a laminate, and in particular, examines and determines specific components contained in biological fluids such as blood, urine, saliva, and interstitial fluid, in particular, in various trace amounts of liquid samples. The present invention relates to a laminate suitable for a biosensor used in The laminate of the present invention is provided with a cavity into which the liquid sample is introduced, so that the amount of liquid sample used can be reduced and the manufacturing process can be simplified.

Biosensors are used to examine and quantify specific components in various trace amounts of liquid samples, for example, specific components contained in biological fluids such as blood, urine, saliva, and interstitial fluid. Although various configurations of biosensors have been proposed, any configuration has a cavity into which a liquid sample is introduced. This cavity consists of two members generally called a spacer and a cover, and a base material A (hereinafter sometimes referred to as a spacer base material) used for the spacer and a cover material B or a cover material C (hereinafter, cover material B) Alternatively, each of the cover materials C or both may be collectively referred to as a cover material.) May be formed by bonding through a resin layer.
In biosensors, the reduction of the amount of biological fluid used as a liquid sample, such as blood, urine, saliva, and interstitial fluid, is emphasized as one method to reduce the burden on patients when using the sensor. It has been attempted by reducing the volume of the cavity.
For example, a configuration is known in which adhesive layers are provided on both surfaces of a spacer substrate, one surface is bonded with a hydrophilic cover, and the other surface is bonded with a substrate on which an electrode is formed (see Patent Document 1). ). However, in the present method, not only the thickness of the spacer base material but also the thickness of the adhesive layer formed on both sides increase the volume of the cavity, so that the burden on the patient is large.

Japanese Patent Application Laid-Open No. 2002-214187

SUMMARY OF THE INVENTION In view of the background of the prior art, the present invention is to provide a laminate suitable for a biosensor in which the amount of liquid sample used is reduced and the manufacturing process is simplified.

The present invention adopts the following means in order to solve the problems.
(1) A laminate having a cover material B, a resin layer X, a base material A, a resin layer Y and a cover material C in this order, which has a cavity having an inlet in the laminate, and the cavity is A laminate comprising the following (i) surface, (ii) surface and (iii) surface.
(I) One surface composed of resin layer X (1) One surface composed of resin layer Y (iii) Three surfaces composed only of base material A (2) Cover material B, resin layer X, base material A, a laminate having a resin layer Y and a cover material C in this order, which has a cavity having an inlet in the laminate, and the cavity has the following surface (iv), the surface of (v) And a laminate comprising the surface of (vi).
(Iv) One surface composed of resin layer X (3) Three surfaces composed only of base material A and resin layer Y (vi) One surface composed of cover material C (3) The resin layer X or resin The laminated body as described in (1) or (2) whose water contact angle of the surface of the at least one resin layer of layer Y is less than 15 degrees.
(4) The adhesive strength according to JIS Z 0237: 2009 between the base material A and the resin layer Y according to any one of (1) to (3), wherein the adhesive strength at a peel angle of 90 ° is 2.5 N / 10 mm or more. Stacks.
(5) The laminate according to any one of (1) to (4), wherein a surfactant is contained in the resin layer X and / or the resin layer Y.
(6) A group in which the resin layer X and / or the resin layer Y is made of polyester resin, (meth) acrylic resin, polyolefin resin, ethylene-vinyl acetate copolymer resin, polyamide resin, chloroprene resin, aramid resin and acrylic urethane resin The laminate according to any one of (1) to (5), which contains at least one resin selected from the group consisting of
(7) The laminate according to (5) or (6), wherein the surfactant is a nonionic surfactant having a polyalkylene glycol skeleton.

According to the present invention, in view of the background of the prior art, the thickness of the spacer is reduced by arranging the resin layer, which has been conventionally arranged as a spacer, as a cover, which is required when introducing the liquid sample. It is possible to reduce the liquid sample. Also, in the conventional configuration shown in FIG. 6, a layer provided with hydrophilicity was provided alone, but by providing hydrophilicity to the resin layer, the layer can simultaneously carry hydrophilicity and adhesiveness. Since integration is possible, the manufacturing process can be simplified.
Furthermore, since the present invention is configured to have the resin layer on both sides of the base material A, the contact area of the liquid sample and the resin layer is increased compared to the prior art. Therefore, particularly when the resin layer is hydrophilic, this can improve the introduction speed of the liquid sample.

FIG. 1 is a diagram showing the configuration of the

laminates

1 and 2 in the first and second embodiments. FIG. 2 is a perspective view of the laminate in FIG. FIG. 3 is a diagram showing a configuration corresponding to the stacks 3 to 18 in Examples 3 to 18. FIG. 4 is a perspective view of the laminate in FIG. FIG. 5 is a conceptual view of a cavity. FIG. 6 is a diagram showing a conventional configuration (a diagram showing the configuration of the stacked body 19 in Comparative Example 1).

The present invention will be described in detail below along with preferred embodiments. The present invention relates to a laminate suitable for a biosensor, which is a laminate having a cover material B, a resin layer X, a base material A, a resin layer Y and a cover material C in this order, in the laminate It is a laminate having a cavity provided with an inlet, wherein the cavity is composed of a specific surface.

In the present invention, the surface constituting the cavity is constituted only by (i) one surface constituted by the resin layer X, (ii) one surface constituted by the resin layer Y, and (iii) the base material A. The aspect composed of three faces is referred to as Invention 1 and the face constituting the cavity is composed only of (iv) one face composed of the resin layer X, (v) the base material A and the resin layer Y An aspect configured of three faces and (vi) one face composed of the cover material C is referred to as invention 2 and the invention 1 and the invention 2 are collectively referred to as the invention.
A biosensor is a device that converts a biological reaction into an electrical signal using an enzyme, a microorganism, or the like. In addition, the biosensor has a cavity and has a structure similar to a laminate or a laminate that sucks a liquid sample from the inlet of the cavity, and is used by being inserted into the measuring instrument main body. Because of their shape, biosensors are also sometimes referred to as sensor chips or chips. By combining and using the biosensor and the measuring instrument body, it becomes possible to quantify the substance to be measured contained in the liquid sample introduced into the cavity. The practical use of sensors using enzymes is in progress, and is being developed and used particularly for medical and health management applications.

The thickness of the base material A used in the present invention is not particularly limited, but from the viewpoint of improving the assembly workability of the laminate, it is preferable to have flexibility because it is preferable to have 500 μm or less. From a viewpoint, 10 micrometers or more are preferable. Furthermore, when a plastic film is used as the substrate A, the thickness of the substrate A is preferably 20 μm to 300 μm, more preferably 30 μm to 250 μm, from the viewpoint of ease of film processing and handling. Moreover, since the thickness of the substrate A is one parameter that defines the volume of the cavity into which the liquid sample is introduced, it is more preferable to be thinner than 200 μm from the viewpoint of reducing the amount of the liquid sample used.

The cavity refers to one provided by completely penetrating at least the base material A in the thickness direction, and is represented by reference numeral 4 in FIGS. The cavity may not only penetrate the base material A in the thickness direction, but also penetrate the resin layer Y in the thickness direction. For example, a cavity penetrating the base material A and the resin layer Y in the thickness direction is represented by reference numeral 4 in FIGS. 3 and 4. The cavity in the present invention, as shown in FIG. 5, is provided with an inlet (reference numeral 41) for introducing a liquid sample, and the cavity 4 is composed of a plurality of faces. As shown in FIGS. 2, 4 and 5, the cavity 4 has an opening on the inlet side, and a liquid sample can be introduced into the cavity 4 from the inlet. Moreover, the opposite side of the inlet of a cavity does not become an opening part, but becomes a surface which comprises a cavity. As a result, it is possible to prevent the liquid sample introduced from the introduction port from overflowing from the opposite side of the introduction port, and further, to the liquid sample at the tip of the cavity (the far end of the cavity, that is, the position far from the introduction port of the cavity). A reagent layer or the like for detecting the composition of 1 and 3 are cross-sectional views of the laminated body cut in the thickness direction between the inlet and the opposite side of the inlet (that is, the surface opposite to the inlet) and viewed from the inlet side. .

Moreover, it is preferable that the base material A has moderate processability in order to form a cavity. In addition, for example, a groove provided by scraping the surface of the base material A (that is, a groove which does not penetrate the base material A in the thickness direction) is not included in the cavity. The method of forming the cavity is not particularly limited, but it is possible to perform physical processing by cutting using scissors or a cutter knife, or by punching using a mold, pinnacle type, Thomson type, engraving type, etc. Alternatively, a known energy beam method represented by laser processing may be used. The cavity may be provided for a configuration in which three layers of the base material A, the resin layer and the silicone resin film described later are laminated, and the cavity may be provided from any surface of the base material A side or the silicone resin film side. It is also good.

There are no particular limitations on the form of the substrate A, cover material B, and cover material C used in the present invention, but for example, plastic films, synthetic paper, paper or composite sheets subjected to surface treatment are preferable, and among them dimensional stability Plastic films are preferred from the viewpoint of durability and the like.
The material of the plastic film is polyester, polyolefin, polyamide, polyesteramide, polyether, polyimide, polyamideimide, polystyrene, polycarbonate, poly-p-phenylene sulfide, polyether ester, polyvinyl chloride, poly (meth) acrylic ester Can be mentioned. Further, these copolymers, blends and further cross-linked compounds can also be used.
Furthermore, among the above-mentioned plastic films, polyester, for example, polyethylene terephthalate, polyethylene 2,6-naphthalate, polyethylene α, β-bis (2-chlorophenoxy)

ethane

4,4′-dicarboxylate, polybutylene terephthalate, etc. A film is preferable, and among them, a film made of polyethylene terephthalate is particularly preferably used in consideration of mechanical properties, quality such as workability, economy and the like comprehensively.

The base material A used in the present invention is preferably a single layer, but for example, a layer having a thickness of 1 μm or less for the purpose of improving the adhesion strength with the adjacent resin layer X and / or resin layer Y It is good also as a laminated structure which has an easily bonding layer) in surface layer.
Between the cover material B and the resin layer X adjacent thereto and / or between the cover material C and the resin layer Y adjacent thereto, a layer different from the resin layers X and Y may be provided as necessary. . By further performing surface treatment such as corona discharge treatment or primer treatment on the surface of different layers, the resin layers X and Y to be laminated on different layers can be formed more firmly. Besides, carbon or gold, between the cover material B and the resin layer X adjacent thereto and / or between the cover material C and the resin layer Y adjacent thereto, in order to function as an electrode material used for a biosensor The electrode layer made of a noble metal such as platinum or palladium may be provided by means of various printing methods such as screen printing or sputtering evaporation method. It is preferable to provide a measurement electrode, a counter electrode, and a detection electrode in the electrode layer provided between the cover material B and the resin layer X adjacent thereto and / or between the cover material C and the resin layer Y adjacent thereto. . When the laminate of the present invention is used as a biosensor, a reagent layer containing an enzyme that specifically reacts with a specific component in a liquid sample, a mediator, and the like may be formed on the electrode layer.

The laminate of the present invention is suitably used as a biosensor. Therefore, a biosensor having the laminate of the present invention has a reagent layer and an electrode layer in addition to the laminate.
Taking a blood glucose level measurement as an example, a method of using a biosensor is shown. After the biosensor with the above configuration is inserted into the measuring instrument body, when blood is brought into contact with the opening of the cavity of the biosensor, blood is drawn into the interior of the cavity and blood is contained in the reagent layer disposed at the tip of the cavity React with the enzyme to generate a current. This current corresponds to the blood glucose level in blood, is transmitted to the measuring instrument body via the mediator and the electrode layer, and is converted to the blood glucose level by the arithmetic device incorporated in the measuring instrument body.

The laminate of the present invention is a laminate having the cover material B and the cover material C on each side of the base material A via the resin layers X and Y, and the cover material C and the base material A are cavities. May be configured.
Moreover, as shown in FIG. 1, the cover film 5 can be comprised by two layers, cover material B21 and resin layer X31, or cover material C22 and resin layer Y32. The laminate 6 of FIG. 1 is a laminate 6 having the cover material B21 and the cover material C22 on the respective surfaces of the base material A1 via the resin layer X31 and the resin layer Y32, and the resin layer X31 together with the base material A1. The resin layer Y 32 constitutes the surface of the cavity 4.
The surfaces 7a to 10b of the cavity into which the liquid sample is introduced are preferably hydrophilic. Hydrophilicity is a measure representing the spread of water when water is dropped on the surface. When hydrophilicity is high, in addition to normal capillary action, the effect of surface tension reduction of the liquid sample is synergistic, and the introduction time is Can be shortened. Therefore, in the laminate structure, the resin layer (for example, the resin layer represented by reference numeral 7a in FIGS. 1 and 3 or 7b in FIG. 1 or 10a or 10b in FIG. High hydrophilicity can be imparted by the following method. Moreover, since the introduction rate to the cavity of a liquid sample improves, so that the area ratio of the surface which has hydrophilicity is large among five surfaces which comprise a cavity, resin which has the hydrophilicity of the front of both surfaces of the base material A earlier More preferably, it is composed of layers. That is, it is preferable that all or part of at least one surface of the cavity be formed of the resin layer X or the resin layer Y. Here, “all or a part of at least one surface of the cavity is formed of the resin layer X or the resin layer Y” means, for example, of five surfaces of the cavity as shown by reference numeral 7a in FIG. When at least one surface is formed of a resin layer (a mode in which all of at least one surface of the cavity is formed of a resin layer X or a resin layer Y), for example, as shown by

reference numerals

10a and 10b in FIG. Such a case where at least one surface of the cavity is formed of the base material A and the resin layer Y (a mode in which a part of at least one surface of the cavity is formed of the resin layer X or the resin layer Y) .

The cavity in the present invention is provided with the inlet for introducing the liquid sample as described above, and the surface constituting the cavity is (i) one surface constituted of the resin layer X, (ii) the resin layer Y Or (iii) only one surface composed of the resin layer X, (v) only the substrate A and the resin layer Y And (vi) one surface composed of a cover material C. When the cavity is composed of a plurality of types of surfaces, adjusting the composition of each surface makes it easy to adjust the hydrophilicity arbitrarily. In addition, the surface that constitutes the cavity does not have to be a flat surface. For example, a curved surface that is curved or a surface that has irregularities is also included in the surface that constitutes the cavity.
Here, in the “one side composed of the resin layer X” in (i) and (iv), as shown by reference numeral 7 a in FIGS. It says to be done. Similarly, “one surface composed of the resin layer Y” in (ii) means that one surface constituting the cavity is composed of the resin layer Y, as indicated by reference numeral 7 b in FIG. 1.

The “three sides composed only of the base material A” in (iii) means that the three sides constituting the cavity consist only of the base material A, as shown by

reference numerals

8a and 8b in FIG. . At this time, for example, the surface constituted by the base material A, the resin layer X and the resin layer Y as indicated by

reference numerals

12a and 12b in FIG. I assume.
The “three faces consisting only of the base material A and the resin layer Y” in (v) means that the three faces constituting the cavity are the base material A and the resin layer Y, as indicated by

reference numerals

10a and 10b in FIG. It consists of only. At this time, for example, in a configuration including another layer between the base material A and the resin layer Y, the three surfaces constituting the cavity include the other layer in addition to the base material A and the resin layer Y. Therefore, it shall not correspond to "3 sides comprised only by the base material A and the resin layer Y."

The “one surface made of the cover material C” in (vi) means that one surface of the cavity is made of the cover material C, as indicated by reference numeral 9 in FIG.
The cavity processes, for example, the configuration of one layer of the base material A or the configuration in which three layers of the base material A, the resin layer X or the resin layer Y, and a silicone resin film described later are laminated in the vertical direction Preferably, it has a rectangular parallelepiped shape to provide it. The width of the cavity is preferably such that the liquid sample is introduced stably and rapidly at capillary action. The width of the cavity depends on the surface tension of the liquid sample and the thickness of the substrate A, but is preferably smaller than 50 mm in order to obtain the effect of capillary action. In addition, the width of the cavity is preferably larger than 0.1 mm because a width capable of introducing a liquid sample is required. The length of the cavity is not particularly limited, but 3 mm or more and 20 mm or less is preferable. If it is shorter than 3 mm, the variation at the time of inspection may increase. If it is longer than 20 mm, not only the introduction speed of the liquid sample will not be stable, but also the volume may be large, so the amount of liquid sample required may be large. The length in the planar direction of the cavity is not particularly limited, but is preferably 10 mm or less, more preferably 7 mm or less, in order to reduce the amount of liquid sample used.

The resin layers X and Y provided on both sides of the substrate A having a cavity are mainly used to bond the surfaces of the two sheets of the substrate A and the cover material B, or the substrate A and the cover material C, respectively. Be The resin layers X and Y used in the present invention are not limited in composition, curing method, solid content, and the like, but preferably exhibit sufficient adhesive strength to the base material A and the cover material B. The resin layers X and Y provided on both sides of the base material A may be the same or different. That is, the resin layers X and Y provided on both sides of the substrate A may have the same composition, curing method, solid content, etc., or the resin layers X and Y may have different compositions. As the composition of the resin layers X and Y, for example, an adhesive or a hot melt adhesive may be used as the component.

The adhesive refers to a coating that adheres an adherend by applying pressure, while the hot melt adhesive refers to a coating that adheres an adherend by heating. Both adhesives and hot melt adhesives are adhesives that are mainly solid and semi-solid at normal temperature and thermoplastic resin that becomes liquid at high temperature, and are in the form of a solution diluted as a coating liquid with water or an organic solvent. May be The term "main component" as used herein refers to a component that occupies 80% by mass or more, based on 100% by mass of the entire pressure-sensitive adhesive or hot melt adhesive, and can be the component that most significantly affects the properties of the hot melt adhesive.
The thermoplastic resins that can be used as the main component of the pressure-sensitive adhesive or hot melt adhesive include polyester resin, (meth) acrylic resin, polyolefin resin, ethylene-vinyl acetate copolymer resin, polyamide resin, chloroprene resin, aramid resin and acrylic urethane It is preferable to include at least one resin selected from the group consisting of resins. In particular, since the hot melt adhesive melts at a low temperature as compared with a thermosetting resin, the heat influence on other members used in the laminate can be suppressed to a small extent. In addition, because of cooling and solidification, bonding can be performed in a short time, and the workability can be improved.

For the hot melt adhesive, for example, the base material A and the cover material B are disposed via a resin layer (hot melt adhesive), a heat sealer is used, sealing temperature 120 ° C., sealing time 1.0 sec, sealing pressure 0 The base material A and the cover material B are adhered under the condition of 2 MPa. For the laminate of the present invention, the adhesive strength between the base material A and the resin layer Y is evaluated three times according to JIS Z 0237: 2009, peeling speed 50 mm / min, 90 ° peeling, and the average value is 2 It is preferable to show an adhesive strength of not less than 5 N / 10 mm, and more preferable to show an adhesive strength of not less than 5 N / 10 mm. If the adhesive strength is less than 2.5 N / 10 mm, stable bonding may not be possible, and peeling of the laminate from the end may occur due to the handling of the laminate.
The resin layer X and / or the resin layer Y used in the present invention comprises polyester resin, (meth) acrylic resin, polyolefin resin, ethylene-vinyl acetate copolymer resin, polyamide resin, chloroprene resin, aramid resin and acrylic urethane resin It is preferred to contain at least one resin selected from the group. Furthermore, in the case of imparting hydrophilicity to the resin layer X and / or the resin layer Y, it is preferable to contain a surfactant. Although there is no limitation on the surfactant to be used, it is preferable to contain a nonionic surfactant having a polyalkylene glycol skeleton (hereinafter, nonionic surfactant having a polyalkylene glycol skeleton is simply used as a surfactant). Sometimes called an agent).

First, as the hot melt adhesive constituting the laminate, it is possible to use a polyester resin or a (meth) acrylic resin as a hot melt adhesive among the above-mentioned groups, as a base material A, a cover material B and a cover material C It is preferable from the viewpoints of adhesive strength of the above and heat resistance. Furthermore, it is particularly preferable to use a polyester resin having a melting point of 40 ° C. or more and 150 ° C. or less or a (meth) acrylic resin as a hot melt adhesive. If the melting point is less than 40 ° C., the hot melt adhesive may flow during heat bonding, and the adhesive may flow to a portion where adhesion is not desired, and may be adhered. If it is greater than 150 ° C., If high temperature is not applied during thermal bonding, bonding may not be possible, resulting in reduced work efficiency or damage to the substrates A, cover material B, and cover material C, which are adherends, due to heat. You may be The hot melt adhesive preferably has an aromatic skeleton because the cohesion of the resin is improved and the adhesive strength of the resin layer is improved. In addition, only one type of resin described above may be used as the hot melt adhesive, or a plurality of resins may be used.

Furthermore, it is preferable that the resin layer X and / or the resin layer Y in the present invention contain a nonionic surfactant having a polyalkylene glycol skeleton together with the above-described hot melt adhesive. The nonionic surfactant having a polyalkylene glycol skeleton used in the present invention has a polyalkylene glycol skeleton as a hydrophilic substituent and a skeleton described later as a hydrophobic substituent. Here, as the nonionic surfactant having a polyalkylene glycol skeleton, for example, polyoxyethylene-alkyl sulfate-sodium salt such as polyoxyethylene lauryl-sulfate sodium salt, polyoxyethylene-lauryl ether, Polyoxyethylene-alkyl ethers such as polyoxyethylene-cetyl ether, polyoxyethylene-oleyl ether, polyoxyethylene-stearyl ether, polyoxyethylene-2-ethylhexyl ether, polyoxyethylene-isodecyl ether, etc.
Polyoxyethylene-alkyl esters such as polyoxyethylene-monolaurate, polyoxyethylene-monostearate, polyoxyethylene-monooleate, polyoxyethylene sorbitan-monolaurate, polyoxyethylene sorbitan-monostearate, polyoxy Sorbitan esters such as ethylene sorbitan-monooleate, polyoxyethylene sorbitan-monooleate, ethylene oxide addition type,
Monoglycerides / ethylene oxide addition type such as polyoxyethylene / coco fatty acid glyceryl,
Triglycerides / ethylene oxide addition type such as polyoxyethylene-hardened castor oil,
Alkylenes such as polyoxyethylene-laurylamine, polyoxyethylene-alkyl (coco) amine, polyoxyethylene-stearylamine, polyoxyethylene-oleylamine, polyoxyethylene-tallow alkylamine, polyoxyethylene alkyl-propylenediamine Polyetheramine-type polyoxyethylene-monomethyl ether, polyoxyethylene-dimethyl ether, polyoxyethylene-glyceryl ether, polyoxyethylene · α, ω-bis-3-aminopropyl-ether, polyethylene glycol-polypropylene glycol-polyethylene glycol ( Surfactants such as block copolymers), but nonionic surfactants having a polyalkylene glycol backbone that can be used in the present invention are It is not this limitation.

Among the nonionic surfactants having a polyalkylene glycol backbone, compounds having an alkyl substituent as a hydrophobic substituent are more preferable. As a concrete example marketed,
Pionin D-1004, Pionin D-1007, Pionin D-1706-N, Pionin D-1715-N, Pionin D-1105, Pionin D-1110, Pionin D-1103-D, Pionin D-1105-D, Pionin D -1103-S, Pionin D-1105-S, Pionin D-1107-S, Pionin D-1109-S, Pionin D-1004, Pionin D-1004, Pionin D-1004,
Newkalgen D-1203, Neucalgen D-1205, Neucalgen D-1208, Pyonin D-1305-Z, Pionin D-1323-Z, Pionin D-1803, Pionin D-1402, Pionin D-1420, Pionin D- 1504, Pionin D-1508, Pionin D-1518, Pionin D-1106 DIR, Pionin D-1110 DIR, Pionin D-1107 SP3, Pionin D-1301-P, Pionin D-1305-P (all manufactured by Takemoto Yushi Co., Ltd.),
Emulgen 102 Kg, Emulgen 103, Emulgen 105, Emulgen 106, Emulgen 108, Emulgen 109 P, Emulgen 120 P, Emulgen 123 P, Emulgen 130 K, Emulgen 147, Emulgen 150, Emulgen 210 P, Emulgen 220 P, Emulgen 306 P, Emulgen 350 P , Emulgen 404, Emulgen 408, Emulgen 409, Emulgen 430, Emulgen 705, Emulgen 707, Emulgen 709, Emulgen 1108, Emulgen 1118 S-70, Emulgen 1135 S-70, Emulgen 1150 S-60, Emulgen 2020 G-HA, Emulgen 2025 G (As described above, manufactured by Kao Corporation), and the like. When the hydrophobic substituent is an alkyl group, it is easy to manufacture and obtain a surfactant having a different carbon number of the alkyl group, so that there is an advantage that the HLB value described later can be arbitrarily changed.

Among the compounds represented by the nonionic surfactant having a polyalkylene glycol skeleton, pionine is a commercially available example of an allylphenyl ether type compound having an arylphenyl group containing an aromatic group as a hydrophobic substituent. D-6112, Neucalgen C-150, Neucalgen C-173, Neucalgen C-200, Neucalgen C-314, Neucalgen CP-50, Neucalgen CP-80, Neucalgen CP-120, Neucalgen CP- 15-200, Pionin D-6512, Pionin D-6414, DTD-51, Pionin D-6315, Neucalgen E-200, Pionin D-7240 (all manufactured by Takemoto Yushi Co., Ltd.), and the like. When the hydrophobic substituent is an allylphenyl group, for example, when a hot melt adhesive having an aromatic group is used in combination, the cohesive strength of the resin layer is improved by the stacking of the hot melt adhesive and the aromatic ring of the surfactant. It is preferable because the adhesive strength is improved. In addition, when the hydrophobic substituent is an allylphenyl group, it is easy to manufacture and obtain a surfactant in which the repeating number of the allylphenyl group is arbitrarily adjusted, so that the HLB value described later can be arbitrarily changed. is there.

Among the compounds represented by nonionic surfactants having the above polyalkylene glycol skeleton, specific examples of commercially available sorbitan fatty acid derivatives include Leodol TW-L120, Leodol TW-L106, Leodol TW-P120, and Leodol TW -S120 V, Reodor TW-S106 V, Reodor TW-S-320 V, Reodor TW-O 120 V, Reodor TW-O 106 V, Reodor TW-O 320 V, Reodor TW-IS 399 C, Reodor Super TW-L 120 (all manufactured by Kao Corporation), Pionin D-941, Pionin D-945, Pionin D-945T (all manufactured by Takemoto Yushi Co., Ltd.) and the like can be mentioned.

The number average molecular weight (Mn) of the nonionic surfactant having the polyalkylene glycol skeleton constituting the laminate is preferably 500 to 20,000, more preferably 2,000 to 20,000, particularly preferably Is 5,000 to 18,000. That is, when the number average molecular weight is 500 or more, the surfactant component adheres to the transport roll or slit blade surface or cross section during the transport process, the printing process, the slit process or the like when rolling the laminate. Can be well prevented, and as a result, the cleaning frequency of each process is significantly reduced, which is preferable. It is preferable that the number average molecular weight is 20,000 or less, since the surfactant can be easily dispersed uniformly in the resin layer.

The melting point or freezing point of the surfactant contained in the resin layer X or the resin layer Y is not particularly limited, but is preferably 30 ° C. or less. The melting point or freezing point is more preferably 25 ° C. or less, still more preferably 20 ° C. or less. If the melting point or the freezing point is higher than 30 ° C., the surfactant is likely to bleed out of the resin layer, and the adhesive strength of the hot melt adhesive may vary, which may result in a decrease in adhesive strength. Moreover, when preparing the coating liquid which mixed the hot melt adhesive and surfactant as melting | fusing point or freezing point is 30 degrees C or less, since the heating-melting operation | work of surfactant becomes unnecessary, it is preferable from a viewpoint of workability | operativity .

In the resin layer X and / or the resin layer Y in the present invention, the amount of surfactant is 0.1 to 100 parts by mass of the resin contained in the resin layer X and the resin layer Y for each of the resin layer X and the resin layer Y. The amount is preferably 20 parts by mass, more preferably 0.1 to 12 parts by mass, and still more preferably 1 to 10 parts by mass. If the amount of surfactant is less than 0.1 parts by mass, the hydrophilicity of the resin layer may not be sufficiently obtained, and the introduction of the liquid sample may not be sufficiently exhibited. Moreover, the effect which prevents that the main component contained in a resin layer adheres in a laminated body conveyance process, a slit process, etc. may not fully be exhibited.
When the amount of surfactant is more than 20 parts by mass, it is difficult to obtain sufficient compatibility with the hot melt adhesive, and the amount of surfactant which bleeds out on the surface of the resin layer increases, and the adhesiveness of the hot melt adhesive is impaired. The desired adhesive strength may not be obtained. If the amount of the surfactant is larger than 20 parts by mass, the surfactant component may be attached in the laminate conveyance step or the slit step due to bleeding out of the surfactant, which may cause contamination of the processing step. In addition, if the amount of surfactant is larger than 20 parts by mass, the compounding ratio of the hot melt adhesive is lowered, the strength of the resin layer is lowered, and stable film formation becomes difficult, and the interface with the hot melt adhesive It may cause a decrease in the storage stability of the paint mixed with the activator. It is preferable that the amount of the surfactant is 0.1 to 20 parts by mass because a resin layer in which the above-mentioned disadvantages are solved can be obtained.

As a hot melt adhesive, for example, Aron melt PES-120L, PES-140H, PES-111EE, PES310S30, PES375S40, PPET 1008, PPET 1025, PPET 2102, PPET 1303 S (all manufactured by Toagosei Co., Ltd.), Y-167, H-930 -S, H-180S (above, manufactured by Tanaka Chemical Co., Ltd.), Nichigo Polyester (R) SP-154, SP-165, SP-170, SP-176, SP-180, SP-182, SP-185 ( Above, manufactured by Nippon Synthetic Chemical Co., Ltd.), Byron (R) 200, 240, 300, 550, BX1001 (above, manufactured by Toyobo Co., Ltd.), Polysol (R) SE-1720, SE-4210 E, SE-6210, SE- 6210L (above, Showa Denko shares Company, Ltd.) and the like.

The water contact angle of the surface of at least one of the resin layer X and the resin layer Y in the present invention is preferably less than 15 degrees. The contact angle to water being less than 15 degrees is preferable because high-speed and stable introduction of a liquid sample can be sufficiently realized. The water contact angle of the surface of at least one of the resin layer X and the resin layer Y is preferably less than 15 degrees, and the water contact angle of the surfaces of both resin layers is more preferably less than 15 degrees.

The surfactant used in the present invention is preferably a surfactant having an HLB value of 8 to 15. The use of a surfactant having an HLB value of 8 to 15 is preferable because hydrophilicity can be imparted to the resin layer while maintaining compatibility with the hot melt adhesive.
The HLB (HyDrophile-Lipophile Balance) value is a hydrophilic-lipophilic balance, and is a value calculated by the Griffin method (all revisions new to surfactant introduction p128) obtained from the following formula (1).
HLB value of surfactant = (number average molecular weight of hydrophilic group portion / number average molecular weight of surfactant) × 20 Formula (1)
When the HLB value of the surfactant is more than 15, it is difficult to obtain sufficient compatibility between the surfactant and the main component of the resin layer, and the amount of the surfactant which bleeds out on the surface of the resin layer increases and the hot melt adhesive Adhesion may be lost and desired adhesion strength may not be obtained. On the other hand, when the HLB value of the surfactant is less than 8, the hydrophilicity of the resin layer may not be sufficiently obtained. It is preferable that the HLB value of the surfactant is 8 to 15, since compatibility with the hot melt adhesive and hydrophilicity of the resin layer can be achieved at the same time.

The adhesion amount of the resin layer X and the resin layer Y is preferably 1 to 50 g / m ² on one side, and more preferably 2 to 30 g / m ² . If the adhesion amount is less than 1 g / m ^{2, the} adhesion layer may come off, pinholes may occur, or desired adhesion strength may not be obtained due to abrasion during processing. As a result, variations in adhesive strength may occur. When the adhesion amount is more than 50 g / m ^2, the effect of preventing the hot melt adhesive component from adhering in the laminate conveyance step, the slit step or the like may be reduced.

Next, means for producing the laminate of the present invention will be described below. The resin layer X and the resin layer Y can be formed, for example, by applying a coating solution containing the components constituting the resin layer to the cover material B and the cover material C to form a coating film. In the case of imparting hydrophilicity, such a coating liquid can be obtained, for example, by mixing a hot melt adhesive and a surfactant, or by heat-melting it.
The coating method of the coating solution is not particularly limited, but methods such as gravure coating, reverse coating, kiss coating, die coating, and bar coating can be used. Although the concentration of the coating solution, the drying condition of the coating film, and the cooling condition of the coating film are not particularly limited, it is desirable that the drying conditions of the coating film be performed in the range which does not adversely affect various characteristics of the substrate.

The resin layer X and the resin layer Y of the present invention can also be formed, for example, by previously forming a film-like material with the above-mentioned coating solution and bonding it to the cover material B or the cover material C. In the case of bonding, for example, a method of applying a coating solution to a release film such as a silicone resin film and transferring it to a cover material is adopted (hereinafter, a two-layer structure of a cover material and a resin layer is a cover film Sometimes called).
Next, although the base material A having a through hole penetrating in the thickness direction is combined with the resin layer X and the resin layer Y previously formed, and heat treatment is performed, the method of heat treatment is not particularly limited. A press or heat sealer (heating roller) can also be used. Then, the laminated body of this invention can be obtained by bonding the cover material B or the cover material C separately prepared also to the back surface of the above-mentioned base material A by heat processing. When the through holes penetrating in the thickness direction are simultaneously provided in the resin layer X and the resin layer Y and the base material A, the coating liquid is formed not on the cover material B or the cover material C but on the base material A A cover film is bonded to the surface on the A side and another cover material is bonded to the resin layer side by heat treatment to obtain a laminate.

EXAMPLES Hereinafter, the present invention will be specifically described by way of examples, but the present invention is not limited by these examples. In addition, in the Example, the evaluation method of the characteristic of a test piece is as follows.

[Contact angle of the surface of the resin layer to water]
The contact angle of the resin layer to water is 5 seconds after dropping of pure water (2.0 μl) onto the surface of the resin layer using DM-400s (manufactured by Kyowa Interface Science Co., Ltd.). The water contact angle of the surface of the resin layer was measured five times, and the average value was taken as the result.

[Adhesive strength of resin layer]
For the laminate, the cover material provided with the resin layer and the base material A are evaluated for adhesive strength three times under the conditions of peeling angle 90 ° and peeling speed 50 mm / min according to JIS Z0237: 2009, and the average value As the result.

[Number average molecular weight of resin]
The molecular weight of polystyrene standard was computed on condition of the following using GPC. Apparatus: HLC-8220GPC (manufactured by Tosoh Corp.), column: TSKgel GMHXL 2 (manufactured by Tosoh Corp.), column temperature: 40 ° C., eluent: tetrahydrofuran 1.00 ml / min, detector: RI.

[Attached amount of resin layer]
The mass of the substrate 100 cm ² coated with the coating solution is measured (X), and then the mass of the substrate 100 cm ² before application is measured (Y), (X−Y) × 100, the adhesion amount (g / m) ² ) calculated.

Qualitative and quantitative determination of surfactant contained in resin layer
Qualitative and quantitative determination of the surfactant contained in the resin layer can be performed by analysis using the LC / MS / MS according to the following procedure. LC / MS / MS is a mass spectrometric technique applicable to non-volatile compounds such as surfactants which are difficult to analyze by LC / MS (gas chromatography mass spectrometer).

<Solution of surfactant contained in resin layer>
(1) Strip the resin layer from the cover film, and weigh 0.04 g of the resin layer into a 25 mL measuring flask.
(2) 1 mL of HFIP (1,1,1,1,3,3,3-hexafluoro-2-propanol) / chloroform (1/1) is added to a volumetric flask to dissolve the resin layer.
(3) After adding 2 mL of chloroform, acetonitrile is gradually added to insolubilize the hot melt adhesive component.
(4) Add acetonitrile to make the volume 25 mL, and dilute the prepared solution 100 times with acetonitrile.
(5) The filtrate obtained by filtering the prepared 100-fold diluted solution with a PTFE disc filter (0.45 μm) is used as a measurement solution.

<Characteristics of surfactant contained in resin layer>
(6) The solution obtained in step (5) is subjected to LC / MS / MS, and the retention time and peak area at which the surfactant-derived peak is detected are confirmed from the chromatogram.
(7) The formula weight of the surfactant-derived positive ion is confirmed by performing MS analysis on the surfactant-derived peak.

<Preparation of standard solution and preparation of calibration curve>
(8) Weigh the standard surfactant preparation (0.01 g) obtained in the procedures (6) and (7) into a 10 mL volumetric flask, dissolve it in methanol, and adjust to 10 mL with the standard solution. Do.
(9) Separate the standard solution and dilute each with methanol to obtain a total of four standard solutions of any concentration.
(10) Each of the standard solutions obtained in the procedure (9) is subjected to LC / MS / MS to confirm the peak area of the chromatogram for each concentration.
(11) A calibration curve is obtained by linear approximation of the relationship between the solution concentration and the peak area.

<Quantitation of surfactant contained in resin layer>
(12) The peak area obtained in step (6) is substituted into the equation of the calibration curve obtained in step (11), and the concentration of surfactant contained in the resin layer is calculated to calculate the content of surfactant Ask for

The procedures (1) to (7) and (11) and (12) are carried out with n = 2, and the average value is taken as the result.
This analysis is performed using LC system: LC-20A (made by Shimadzu Corporation), MS system: API4000 (made by AB SCIEX Co., Ltd.), column: Inertsil ODS-3 (2.1 × 150 mm, 5 μm) (GL Sciences Inc.) Column temperature: 50 ° C., flow rate: 0.25 mL / min, injection amount: 1 μL, ionization method: APCI (atmospheric pressure chemical ionization method), detection: positive ion detection, measurement mode: SRM (Selected reAction monitoring) Conduct under the conditions of).

Example 1
A 20% by mass solution of a polyester-based adhesive resin having a melting point of 100 ° C. and a number average molecular weight of 22,000 dissolved in toluene is prepared as a resin constituting the hot melt adhesive, and a nonionic surfactant having a polyalkylene glycol skeleton [Kao Co., Ltd. Leodol TW-L106 (HLB value: 13.3)] was prepared, and the coating solution was prepared so that the solid content conversion ratio would be 100 parts by mass / 4 parts by mass. This coating solution is applied to a silicone resin coated surface of a silicone resin film by a comma coater, and dried at 120 ° C. for 30 seconds to form a resin layer 1a with an adhesion amount of 45 g / m ² on one side, and then Toray Co., Ltd. Transfer to a company-made polyethylene terephthalate (PET) film "Lumirror" (registered trademark) (type 100S28), and peeling off the silicone resin film to obtain a cover film 1A. The coating thickness of the resin layer 1a was 20 μm.

Next, a through hole penetrating in the thickness direction of 10 mm in width by laser processing (a through hole penetrating in the thickness direction of 10 mm in width by laser processing has a shape like the substrate A indicated by reference numeral 1 in FIG. The resin layer side of the cover film 1A is aligned with the Toray Industries Co., Ltd. polyethylene terephthalate (PET) film “Lumirror” (registered trademark) (type 100E20) provided with the part processed in It stuck on the conditions. The 100E 20 side of the obtained sample and the resin layer side of another cover film 1A prepared separately were combined, and laminated again using the heat sealer under the conditions described above, to obtain a laminate 1 of the present invention. The laminate 1 has a configuration corresponding to FIGS. 1 and 2, and the laminate 1 has the configuration of the aspect of the present invention 1.

(Example 2)
In Example 1, prepare a 20% by mass solution in which a polyester adhesive resin having a melting point of 100 ° C. and a number average molecular weight of 22,000 is dissolved in toluene as a resin constituting the hot melt adhesive in one piece of the resin layer 1a. The laminated body 2 of the present invention was obtained in the same manner except that the resin layer 2b obtained in the above was used. The laminate 2 has a configuration corresponding to FIGS. 1 and 2, and the laminate 2 has the configuration of the aspect of the present invention 1.

(Example 3)
A resin layer 2b is provided on a silicone resin film, and a polyethylene terephthalate (PET) film "Lumirror" (registered trademark) (type 100E20) manufactured by Toray Industries, Inc. is bonded to the surface of the resin layer 2b, and a punching method using a Thomson type And a through hole penetrating in the thickness direction of 10 mm in width. Then, the spacer film 3 was obtained by peeling a silicone type resin film. After bonding the resin layer 2b surface of the obtained spacer film 3 to a polyethylene terephthalate (PET) film "Lumirror" (registered trademark) (type 100S28) manufactured by Toray Industries, Inc. using a heat sealer under the conditions of Example 1 A laminate 3 of the present invention was obtained by bonding the surface of the cover film 1A separately prepared, and the spacer base of the spacer film 3, using a heat sealer under the conditions of Example 1. The laminate 3 has a configuration corresponding to FIGS. 3 and 4, and the laminate 3 has the configuration of the embodiment of the present invention 2.

(Example 4)
In Example 3, using a magnetron sputtering apparatus on the bonding surface side of Toray Industries, Ltd. polyethylene terephthalate (PET) film "Lumirror" (registered trademark) (type 188E20), a palladium layer with a thickness of 10 nm was used as a film base A laminate 4 of the present invention was obtained in the same manner except that the cover material 4 formed on one surface was provided on the resin layer 2b surface of the spacer film 3. In addition, as a target of magnetron sputtering, palladium with a purity of 99.9% by mass (manufactured by Tanaka Kikinzoku Kogyo Co., Ltd.) was used, and argon was used as a sputtering gas. The laminate 4 has a configuration corresponding to FIGS. 3 and 4, and the laminate 4 has the configuration of the aspect of the present invention 2.

(Example 5)
In Example 4, a nonionic surfactant having a polyalkylene glycol skeleton [Leodor TW-L106 (HLB value: 13.3) manufactured by Kao Corp.) is a ratio of the solid content conversion ratio of the hot melt adhesive of 8 parts by mass. A laminate 5 of the present invention was obtained in the same manner except that the coating liquid was prepared as described above. The laminate 5 has a configuration corresponding to FIGS. 3 and 4, and the laminate 5 has the configuration of the aspect of the present invention 2.

(Example 6)
In Example 4, a nonionic surfactant having a polyalkylene glycol skeleton [Pionin D-1508 (HLB value: 11.4) manufactured by Takemoto Yushi Co., Ltd.] in a proportion of 4 parts by mass in terms of solid content conversion of hot melt adhesive The laminate 6 of the present invention was obtained in the same manner as described above except that the coating liquid was prepared. The laminate 6 has a configuration corresponding to FIGS. 3 and 4, and the laminate 6 has the configuration of the embodiment of the present invention 2.

(Example 7)
In Example 4, a nonionic surfactant having a polyalkylene glycol skeleton [Pionin D-1508 (HLB value: 11.4), manufactured by Takemoto Yushi Co., Ltd.] was added to a proportion of 3 parts by mass of the solid content of the hot melt adhesive. The laminate 7 of the present invention was obtained in the same manner except that the coating liquid was prepared to be The laminate 7 has a configuration corresponding to FIGS. 3 and 4, and the laminate 7 has the configuration of the aspect of the present invention 2.

(Example 8)
In Example 4, a nonionic surfactant having a polyalkylene glycol skeleton [Pionin D-1508 (HLB value: 11.4) manufactured by Takemoto Yushi Co., Ltd.] in a proportion of 20 parts by mass in terms of the solid content conversion of the hot melt adhesive The laminate 8 of the present invention was obtained in the same manner except that the coating liquid was prepared to be The laminate 8 has a configuration corresponding to FIGS. 3 and 4, and the laminate 8 has the configuration of the aspect of the present invention 2.

(Example 9)
In Example 4, a nonionic surfactant having a polyalkylene glycol skeleton [Pionin D-1105-S (HLB value: 10.5) manufactured by Takemoto Oil & Fats Co., Ltd.] was converted to a solid content ratio of 4 parts by mass of the hot melt adhesive. A laminate 9 of the present invention was obtained in the same manner except that the coating liquid was prepared to have a ratio. The laminate 9 has a configuration corresponding to FIGS. 3 and 4, and the laminate 9 has the configuration of the aspect of the present invention 2.

(Example 10)
In Example 4, a nonionic surfactant having a polyalkylene glycol skeleton [Pionin D-1105-S (HLB value: 10.5) manufactured by Takemoto Oil & Fats Co., Ltd.] was converted to a solid content ratio of 8 parts by mass of the hot melt adhesive. A laminate 10 of the present invention was obtained in the same manner except that the coating liquid was prepared to have a ratio. The laminate 10 has a configuration corresponding to FIGS. 3 and 4, and the laminate 10 has the configuration of the aspect of the second invention.

(Example 11)
In Example 4, a nonionic surfactant having a polyalkylene glycol skeleton (Emulgen 103 (HLB value: 8.1) manufactured by Kao Corp.) is made to have a ratio of 4 parts by mass of the solid content conversion ratio of the hot melt adhesive A laminate 11 of the present invention was obtained in the same manner except that the coating solution was prepared. The laminate 11 has a configuration corresponding to FIGS. 3 and 4, and the laminate 11 has the configuration of the aspect of the present invention 2.

(Example 12)
In Example 4, a nonionic surfactant having a polyalkylene glycol skeleton [Emulgen 103 (HLB value: 8.1) manufactured by Kao Corp.] is made to have a ratio of the solid content conversion ratio of the hot melt adhesive of 8 parts by mass. A laminate 12 of the present invention was obtained in the same manner except that a coating solution was prepared. The laminate 12 has a configuration corresponding to FIGS. 3 and 4, and the laminate 12 has the configuration of the aspect of the present invention 2.

(Example 13)
In Example 4, the proportion of the nonionic surfactant having a polyalkylene glycol skeleton [Nucargen D-1205 (HLB value: 10.5) manufactured by Takemoto Yushi Co., Ltd.] to the solid content conversion ratio of the hot melt adhesive is 4 parts by mass The laminate 13 of the present invention was obtained in the same manner except that the coating liquid was prepared to become The laminate 13 has a configuration corresponding to FIGS. 3 and 4, and the laminate 13 has the configuration of the aspect of the present invention 2.

(Example 14)
In Example 4, the ratio of the solid content conversion ratio of the hot melt adhesive to 8 parts by mass of a nonionic surfactant having a polyalkylene glycol skeleton [Nucargen D-1205 (HLB value: 10.5) manufactured by Takemoto Yushi Co., Ltd.] The laminate 14 of the present invention was obtained in the same manner except that the coating liquid was prepared to be The laminate 14 has a configuration corresponding to FIGS. 3 and 4, and the laminate 14 has the configuration of the aspect of the present invention 2.

(Example 15)
In Example 4, the nonionic surfactant having a polyalkylene glycol skeleton [DTD-51 (HLB value: 13.7), manufactured by Takemoto Yushi Co., Ltd.] is a ratio of 4 parts by mass of the solid content conversion ratio of the hot melt adhesive A laminate 15 of the present invention was obtained in the same manner as described above except that the coating solution was prepared. The laminate 15 has a configuration corresponding to FIGS. 3 and 4, and the laminate 15 has the configuration of the aspect of the present invention 2.

(Example 16)
In Example 4, the nonionic surfactant having a polyalkylene glycol skeleton [DTD-51 (HLB value: 13.7) manufactured by Takemoto Oil & Fats Co., Ltd.] is a ratio of the solid content conversion ratio of the hot melt adhesive of 8 parts by mass. A laminate 16 of the present invention was obtained in the same manner except that the coating liquid was prepared as described above. The laminate 16 has a configuration corresponding to FIGS. 3 and 4, and the laminate 16 has the configuration of the aspect of the second invention.

(Example 17)
In Example 4, a nonionic surfactant having a polyalkylene glycol skeleton [Pionin D-1007 (HLB value: 14.1), manufactured by Takemoto Yushi Co., Ltd.] in a proportion of 4 parts by mass of the solid content conversion ratio of the hot melt adhesive The laminate 17 of the present invention was obtained in the same manner except that the coating solution was prepared to be The laminate 17 has a configuration corresponding to FIGS. 3 and 4, and the laminate 17 has the configuration of the aspect of the second invention.

(Example 18)
In Example 4, a nonionic surfactant having a polyalkylene glycol skeleton [Pionin D-1007 (HLB value: 14.1), manufactured by Takemoto Oil & Fats Co., Ltd.] in a ratio of 8 parts by mass in terms of solid content conversion of hot melt adhesive The laminate 18 of the present invention was obtained in the same manner except that the coating liquid was prepared to be The laminate 18 has a configuration corresponding to FIGS. 3 and 4, and the laminate 18 has the configuration of the aspect of the present invention 2.

(Comparative example 1)
Cationic surfactant (Toho Chemical Industry Co., Ltd. Anstex C-200X) per 100 parts by mass of polyester resin onto polyethylene terephthalate (PET) film "Lumirror" (registered trademark) (type 100S28) manufactured by Toray Industries, Inc. Was prepared in a proportion of 5 parts by mass, and was coated by a gravure coater to obtain a cover film 19A having the hydrophilic coating layer 11.
Next, a polyester adhesive resin is applied to the silicone resin coated surface of the silicone resin film with a comma coater to form a hot melt resin layer, and then a polyethylene terephthalate (PET) film "Lumirror" manufactured by Toray Industries, Inc. (registration Transferred to trademark (type 100E20). A hot melt resin layer was provided on the silicone resin coated surface of the silicone resin film by the same procedure, and the hot melt resin layer was transferred to the other surface of the previous 100E20. Furthermore, the spacer film 19B was obtained by providing the through-hole penetrated in the thickness direction.
Next, a palladium layer was formed on one side of a polyethylene terephthalate (PET) film "Lumirror" (registered trademark) (type 188E20) manufactured by Toray Industries, Inc. using a magnetron sputtering apparatus.
The hydrophilic coated surface of the cover film 19A and the hot melt resin layer surface of the spacer film 19B were bonded using a heat sealer under the conditions described above. The other hot-melt resin layer surface of the obtained sample and the palladium surface described above were combined and bonded again using the heat sealer under the above-described conditions, to obtain a laminate 19 of the present invention. The laminated body 19 is a structure which has the hydrophilic-coat layer 11 corresponding to FIG. 6, and does not have the structure of the laminated body prescribed | regulated by

Claim

1 and 2. FIG. FIG. 6 is a cross-sectional view of the laminate cut in the thickness direction between the inlet and the opposite side of the inlet (that is, the surface opposite to the inlet) and viewed from the inlet side.
When the thickness of each of the base material A, the resin layer X and the resin layer Y is constant, Examples 1 to 18 corresponding to FIG. 3 keep the height of the cavity low compared to Comparative Example 1 corresponding to FIG. Therefore, the amount of liquid sample used can be reduced. Further, as compared with Comparative Example 1, in Examples 1 to 18, since the hydrophilic coat layer is not provided, the manufacturing process is simplified.

According to the present invention, it is possible to provide a laminate suitable for a biosensor in which the amount of liquid sample used is reduced and the manufacturing process is simplified.

Although the invention has been described in detail and with reference to specific embodiments, it will be apparent to those skilled in the art that various changes and modifications can be made without departing from the spirit and scope of the invention.
This application is based on Japanese Patent Application (Japanese Patent Application No. 2017-203166) filed on October 20, 2017, the contents of which are incorporated herein by reference.

1: Substrate A (Spacer substrate)
21: Cover material B
22: Cover material C
31: Resin layer X
32: Resin layer Y
4: Cavity 41: Inlet 5: Cover film 6: Laminate 7a: Surface 7b composed of resin layer X: Surface 8a composed of resin layer Y: Surface 9 composed only of base material A: 9

Surfaces

10a and 10b composed of the cover material C: Surfaces composed only of the base material A and the resin layer Y: Hydrophilic coated layers 12a and 12b: faces composed of the substrate A, resin layer X and resin layer Y

Claims

It is a laminate having a cover material B, a resin layer X, a base material A, a resin layer Y and a cover material C in this order, and the laminate has a cavity having an inlet port, A laminate comprising the surface of i), the surface of (ii) and the surface of (iii).
(I) One side composed of the resin layer X (ii) One side composed of the resin layer Y (iii) Three sides composed only of the base material A
It is a laminate having a cover material B, a resin layer X, a base material A, a resin layer Y and a cover material C in this order, and the laminate has a cavity having an inlet port, A laminate comprising the surface of iv), the surface of (v) and the surface of (vi).
(Iv) One Surface Composed of Resin Layer X (V) Three Surface Composed Only of Substrate A and Resin Layer Y (Vi) One Surface Constructed of Cover Material C
The laminated body according to claim 1 or 2, wherein a water contact angle of a surface of at least one of the resin layer X and the resin layer Y is less than 15 degrees.
The laminate according to any one of claims 1 to 3, wherein the adhesive strength between the base material A and the resin layer Y in accordance with JIS Z0237: 2009 is at least 2.5 N / 10 mm. .
The laminate according to any one of claims 1 to 4, wherein the resin layer X and / or the resin layer Y contains a surfactant.
The resin layer X and / or the resin layer Y is selected from the group consisting of polyester resin, (meth) acrylic resin, polyolefin resin, ethylene-vinyl acetate copolymer resin, polyamide resin, chloroprene resin, aramid resin and acrylic urethane resin The laminate according to any one of claims 1 to 5, which contains at least one resin.
The laminate according to claim 5 or 6, wherein the surfactant is a nonionic surfactant having a polyalkylene glycol skeleton.